{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T05:48:55Z","timestamp":1784958535040,"version":"3.55.0"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:00:00Z","timestamp":1768780800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:00:00Z","timestamp":1768780800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur Biophys J"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1007\/s00249-025-01812-5","type":"journal-article","created":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T06:46:30Z","timestamp":1768805190000},"page":"55-62","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Achieving femtomolar affinities in structure-based drug design"],"prefix":"10.1007","volume":"55","author":[{"given":"Asta","family":"Zubrien\u0117","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maija","family":"Kurtenoka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vaida","family":"Paketuryt\u0117-Latv\u0117","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janis","family":"Leitans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Manakova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mantas","family":"\u017dvirblis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andris","family":"Kazaks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladislava","family":"Eimonta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaspars","family":"Tars","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saulius","family":"Gra\u017eulis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vytautas","family":"Petrauskas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jurgita","family":"Matulien\u0117","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginija","family":"Dudutien\u0117","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirill","family":"Shubin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6178-6276","authenticated-orcid":false,"given":"Daumantas","family":"Matulis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,1,19]]},"reference":[{"key":"1812_CR6","doi-asserted-by":"publisher","first-page":"112547","DOI":"10.1016\/j.jinorgbio.2024.112547","volume":"256","author":"D Baronas","year":"2024","unstructured":"Baronas D, Kna\u0161ien\u0117 B, Mickevi\u010di\u016bt\u0117 A, Jachno J, Naujalis E, Zubrien\u0117 A, Matulis D (2024) Inhibitor binding to Metal-Substituted metalloenzyme: sulfonamide affinity for carbonic anhydrase IX. J Inorg Biochem 256:112547. https:\/\/doi.org\/10.1016\/j.jinorgbio.2024.112547","journal-title":"J Inorg Biochem"},{"key":"1812_CR31","doi-asserted-by":"publisher","unstructured":"Copeland RA (2013) Tight binding Inhibition. Evaluation of enzyme inhibitors in drug discovery. John Wiley & Sons, Ltd, pp 245\u2013285. https:\/\/doi.org\/10.1002\/9781118540398.ch7.","DOI":"10.1002\/9781118540398.ch7"},{"issue":"1","key":"1812_CR8","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1021\/acs.chemrev.4c00278","volume":"125","author":"K D\u2019Ambrosio","year":"2025","unstructured":"D\u2019Ambrosio K, Di Fiore A, Alterio V, Langella E, Monti SM, Supuran CT, De Simone G (2025) Multiple binding modes of inhibitors to human carbonic anhydrases: an update on the design of Isoform-Specific modulators of activity. Chem Rev 125(1):150\u2013222. https:\/\/doi.org\/10.1021\/acs.chemrev.4c00278","journal-title":"Chem Rev"},{"issue":"2","key":"1812_CR12","doi-asserted-by":"publisher","first-page":"404","DOI":"10.1016\/j.bbapap.2009.07.027","volume":"1804","author":"G De Simone","year":"2010","unstructured":"De Simone G, Supuran CT, Carbonic Anhydrase IX (2010) Biochemical and crystallographic characterization of a novel antitumor target. Biochim Biophys Acta BBA - Proteins Proteom 1804(2):404\u2013409. https:\/\/doi.org\/10.1016\/j.bbapap.2009.07.027","journal-title":"Biochim Biophys Acta BBA - Proteins Proteom"},{"issue":"22","key":"1812_CR23","doi-asserted-by":"publisher","first-page":"9435","DOI":"10.1021\/jm501003k","volume":"57","author":"V Dudutien\u0117","year":"2014","unstructured":"Dudutien\u0117 V, Matulien\u0117 J, Smirnov A, Timm DD, Zubrien\u0117 A, Baranauskien\u0117 L, Mork\u016bnaite V, Smirnovien\u0117 J, Michailovien\u0117 V, Juozapaitien\u0117 V, Mickevi\u010di\u016bt\u0117 A, Kazokait\u0117 J, Bak\u0161yt\u0117 S, Kasiliauskait\u0117 A, Jachno J, Revuckien\u0117 J, Ki\u0161onait\u0117 M, Pilipuityt\u0117 V, Ivanauskait\u0117 E, Milinavi\u010di\u016bt\u0117 G, Smirnovas V, Petrikait\u0117 V, Kairys V, Petrauskas V, Norvai\u0161as P, Ling\u0117 D, Gibie\u017ea P, Capkauskait\u0117 E, Zak\u0161auskas A, Kazlauskas E, Manakova E, Gra\u017eulis S, Ladbury JE, Matulis D (2014) Discovery and characterization of novel selective inhibitors of carbonic anhydrase IX. J Med Chem 57(22):9435\u20139446. https:\/\/doi.org\/10.1021\/jm501003k","journal-title":"J Med Chem"},{"key":"1812_CR1","doi-asserted-by":"publisher","unstructured":"Huggins DJ, Sherman W, Tidor B (2012) Rational Approaches to Improving Selectivity in Drug Design. J. Med. Chem. 55 (4):1424\u20131444. https:\/\/doi.org\/10.1021\/jm2010332","DOI":"10.1021\/jm2010332"},{"issue":"0","key":"1812_CR5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/17460441.2024.2349169","volume":"0","author":"V Kairys","year":"2024","unstructured":"Kairys V, Baranauskiene L, Kazlauskiene M, Zubrien\u0117 A, Petrauskas V, Matulis D, Kazlauskas E (2024) Recent advances in computational and experimental Protein-Ligand affinity determination techniques. Expert Opin Drug Discov 0(0):1\u201322. https:\/\/doi.org\/10.1080\/17460441.2024.2349169","journal-title":"Expert Opin Drug Discov"},{"key":"1812_CR20","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1016\/j.ymeth.2014.10.034","volume":"76","author":"G Krainer","year":"2015","unstructured":"Krainer G, Keller S (2015) Single-Experiment displacement assay for quantifying High-Affinity binding by isothermal Titration calorimetry. Methods 76:116\u2013123. https:\/\/doi.org\/10.1016\/j.ymeth.2014.10.034","journal-title":"Methods"},{"issue":"3","key":"1812_CR7","doi-asserted-by":"publisher","first-page":"946","DOI":"10.1021\/cr050262p","volume":"108","author":"VM Krishnamurthy","year":"2008","unstructured":"Krishnamurthy VM, Kaufman GK, Urbach AR, Gitlin I, Gudiksen KL, Weibel DB, Whitesides GM (2008) Carbonic anhydrase as a model for biophysical and Physical-Organic studies of proteins and Protein\u2009\u2013\u2009Ligand binding. Chem Rev 108(3):946\u20131051. https:\/\/doi.org\/10.1021\/cr050262p","journal-title":"Chem Rev"},{"key":"1812_CR2","doi-asserted-by":"publisher","unstructured":"Kuntz ID, Chen K, Sharp KA, Kollman PA (1999) The Maximal Affinity of Ligands. Proc. Natl. Acad. Sci. 96(18):9997\u201310002. https:\/\/doi.org\/10.1073\/pnas.96.18.9997","DOI":"10.1073\/pnas.96.18.9997"},{"key":"1812_CR16","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.ab.2016.10.005","volume":"515","author":"V Linkuvien\u0117","year":"2016","unstructured":"Linkuvien\u0117 V, Krainer G, Chen W-Y, Matulis D (2016a) Isothermal Titration calorimetry for drug design: precision of the enthalpy and binding constant measurements and comparison of the instruments. Anal Biochem 515:61\u201364. https:\/\/doi.org\/10.1016\/j.ab.2016.10.005","journal-title":"Anal Biochem"},{"issue":"4","key":"1812_CR17","doi-asserted-by":"publisher","first-page":"708","DOI":"10.1016\/j.bbagen.2016.01.007","volume":"1860","author":"V Linkuvien\u0117","year":"2016","unstructured":"Linkuvien\u0117 V, Matulien\u0117 J, Juozapaitien\u0117 V, Michailovien\u0117 V, Jachno J, Matulis D (2016b) Intrinsic thermodynamics of inhibitor binding to human carbonic anhydrase IX. Biochim Biophys Acta BBA - Gen Subj 1860(4):708\u2013718. https:\/\/doi.org\/10.1016\/j.bbagen.2016.01.007","journal-title":"Biochim Biophys Acta BBA - Gen Subj"},{"key":"1812_CR15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1017\/S0033583518000082","volume":"51","author":"V Linkuvien\u0117","year":"2018","unstructured":"Linkuvien\u0117 V, Zubrien\u0117 A, Manakova E, Petrauskas V, Baranauskien\u0117 L, Zak\u0161auskas A, Smirnov A, Gra\u017eulis S, Ladbury JE, Matulis D, Thermodynamic (2018a) Kinetic, and structural parameterization of human carbonic anhydrase interactions toward enhanced inhibitor design. Q Rev Biophys 51:1\u201348. https:\/\/doi.org\/10.1017\/S0033583518000082","journal-title":"Q Rev Biophys"},{"issue":"6","key":"1812_CR21","doi-asserted-by":"publisher","first-page":"2292","DOI":"10.1021\/acs.jmedchem.7b01408","volume":"61","author":"V Linkuvien\u0117","year":"2018","unstructured":"Linkuvien\u0117 V, Talibov VO, Danielson UH, Matulis D (2018b) Introduction of intrinsic kinetics of Protein\u2013Ligand interactions and their implications for drug design. J Med Chem 61(6):2292\u20132302. https:\/\/doi.org\/10.1021\/acs.jmedchem.7b01408","journal-title":"J Med Chem"},{"issue":"1","key":"1812_CR25","doi-asserted-by":"publisher","first-page":"17644","DOI":"10.1038\/s41598-022-22436-1","volume":"12","author":"J Matulien\u0117","year":"2022","unstructured":"Matulien\u0117 J, \u017dvinys G, Petrauskas V, Kvietkauskait\u0117 A, Zak\u0161auskas A, Shubin K, Zubrien\u0117 A, Baranauskien\u0117 L, Ka\u010denauskait\u0117 L, Kopanchuk S, Veiksina S, Paketuryt\u0117-Latv\u0117 V, Smirnovien\u0117 J, Juozapaitien\u0117 V, Mickevi\u010di\u016bt\u0117 A, Michailovien\u0117 V, Jachno J, Stravinskien\u0117 D, Sli\u017eien\u0117 A, Petro\u0161i\u016bt\u0117 A, Becker HM, Kazokait\u0117-Adomaitien\u0117 J, Yaromina A, \u010capkauskait\u0117 E, Rinken A, Dudutien\u0117 V, Dubois LJ, Matulis D (2022) Picomolar fluorescent probes for compound affinity determination to carbonic anhydrase IX expressed in live cancer cells. Sci Rep 12(1):17644. https:\/\/doi.org\/10.1038\/s41598-022-22436-1","journal-title":"Sci Rep"},{"issue":"22","key":"1812_CR27","doi-asserted-by":"publisher","first-page":"16319","DOI":"10.1021\/acs.jmedchem.1c00975","volume":"64","author":"DB McConnell","year":"2021","unstructured":"McConnell DB (2021) Biotin\u2019s lessons in drug design. J Med Chem 64(22):16319\u201316327. https:\/\/doi.org\/10.1021\/acs.jmedchem.1c00975","journal-title":"J Med Chem"},{"key":"1812_CR28","doi-asserted-by":"publisher","unstructured":"McDonald PC, Chia S, Bedard PL, Chu Q, Lyle M, Tang L, Singh M, Zhang Z, Supuran CT, Renouf DJ, Dedhar SA (2020) Phase 1 study of SLC-0111, a novel inhibitor of carbonic anhydrase IX, in patients with advanced solid tumors. Am J Clin Oncol. https:\/\/doi.org\/10.1097\/COC.0000000000000691","DOI":"10.1097\/COC.0000000000000691"},{"key":"1812_CR13","doi-asserted-by":"publisher","unstructured":"Mickevi\u010di\u016bt\u0117 A, Juozapaitien\u0117 V, Michailovien\u0117 V, Jachno J, Matulien\u0117 J, Matulis D (2019) Recombinant production of 12 catalytically active human CA isoforms. In: Matulis D (ed) Carbonic anhydrase as drug target: thermodynamics and structure of inhibitor binding. Springer International Publishing: Cham, pp 15\u201337. https:\/\/doi.org\/10.1007\/978-3-030-12780-0_2.","DOI":"10.1007\/978-3-030-12780-0_2"},{"issue":"4","key":"1812_CR9","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1002\/ijc.2910380406","volume":"38","author":"E Oosterwijk","year":"1986","unstructured":"Oosterwijk E, Ruiter DJ, Hoedemaeker PJ, Pauwels EK, Jonas U, Zwartendijk J, Warnaar SO (1986) Monoclonal antibody G 250 recognizes a determinant present in Renal-Cell carcinoma and absent from normal kidney. Int J Cancer 38(4):489\u2013494. https:\/\/doi.org\/10.1002\/ijc.2910380406","journal-title":"Int J Cancer"},{"issue":"10","key":"1812_CR10","first-page":"2877","volume":"9","author":"J Pastorek","year":"1994","unstructured":"Pastorek J, Pastorekov\u00e1 S, Callebaut I, Mornon JP, Zeln\u00edk V, Opavsk\u00fd R, Zat\u2019ovicov\u00e1 M, Liao S, Portetelle D, Stanbridge EJ (1994) Cloning and characterization of MN, a human Tumor-Associated protein with a domain homologous to carbonic anhydrase and a putative Helix-Loop-Helix DNA binding segment. Oncogene 9(10):2877\u20132888","journal-title":"Oncogene"},{"key":"1812_CR19","doi-asserted-by":"publisher","first-page":"117417","DOI":"10.1016\/j.trac.2023.117417","volume":"170","author":"V Petrauskas","year":"2024","unstructured":"Petrauskas V, Kazlauskas E, Gedgaudas M, Baranauskien\u0117 L, Zubrien\u0117 A, Matulis D (2024) Thermal shift assay for Protein\u2013Ligand dissociation constant determination. TrAC Trends Anal Chem 170:117417. https:\/\/doi.org\/10.1016\/j.trac.2023.117417","journal-title":"TrAC Trends Anal Chem"},{"issue":"7","key":"1812_CR26","doi-asserted-by":"publisher","first-page":"1610","DOI":"10.1111\/bph.17429","volume":"182","author":"A Petrosiute","year":"2025","unstructured":"Petrosiute A, Zak\u0161auskas A, Lu\u010di\u016bnait\u0117 A, Petrauskas V, Baranauskien\u0117 L, Kvietkauskait\u0117 A, \u0160\u010derbavi\u010dien\u0117 A, Tamo\u0161i\u016bnait\u0117 M, Musvicait\u0117 J, Jank\u016bnait\u0117 A, \u017dvinys G, Stan\u010daitis L, \u010capkauskait\u0117 E, Mickevi\u010di\u016bt\u0117 A, Juozapaitien\u0117 V, Dudutien\u0117 V, Zubrien\u0117 A, Grincevi\u010dien\u0117 \u0160, Bukelskien\u0117 V, Schi\u00f6th HB, Matulien\u0117 J, Matulis D (2025) Carbonic anhydrase IX Inhibition as a path to treat neuroblastoma. Br J Pharmacol 182(7):1610\u20131629. https:\/\/doi.org\/10.1111\/bph.17429","journal-title":"Br J Pharmacol"},{"issue":"1","key":"1812_CR11","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1016\/S0002-9440(10)65569-1","volume":"153","author":"J Saarnio","year":"1998","unstructured":"Saarnio J, Parkkila S, Parkkila A-K, Haukipuro K, Pastorekov\u00e1 S, Pastorek J, Kairaluoma MI, Karttunen TJ (1998) Immunohistochemical study of colorectal tumors for expression of a novel transmembrane carbonic Anhydrase, MN\/CA IX, with potential value as a marker of cell proliferation. Am J Pathol 153(1):279\u2013285. https:\/\/doi.org\/10.1016\/S0002-9440(10)65569-1","journal-title":"Am J Pathol"},{"issue":"2","key":"1812_CR22","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1006\/abio.1999.4402","volume":"277","author":"BW Sigurskjold","year":"2000","unstructured":"Sigurskjold BW (2000) Exact analysis of competition ligand binding by displacement isothermal Titration calorimetry. Anal Biochem 277(2):260\u2013266. https:\/\/doi.org\/10.1006\/abio.1999.4402","journal-title":"Anal Biochem"},{"key":"1812_CR30","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.ab.2017.01.022","volume":"522","author":"J Smirnovien\u0117","year":"2017","unstructured":"Smirnovien\u0117 J, Smirnovas V, Matulis D (2017) Picomolar inhibitors of carbonic anhydrase: importance of Inhibition and binding assays. Anal Biochem 522:61\u201372. https:\/\/doi.org\/10.1016\/j.ab.2017.01.022","journal-title":"Anal Biochem"},{"issue":"2","key":"1812_CR3","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1021\/jm800498e","volume":"52","author":"AJT Smith","year":"2009","unstructured":"Smith AJT, Zhang X, Leach AG, Houk KN (2009) Beyond picomolar affinities: quantitative aspects of noncovalent and covalent binding of drugs to proteins. J Med Chem 52(2):225\u2013233. https:\/\/doi.org\/10.1021\/jm800498e","journal-title":"J Med Chem"},{"issue":"8","key":"1812_CR4","doi-asserted-by":"publisher","first-page":"2098","DOI":"10.1021\/ci200612f","volume":"52","author":"RD Smith","year":"2012","unstructured":"Smith RD, Engdahl AL, Dunbar JB, Carlson HA (2012) Biophysical limits of Protein\u2013Ligand binding. J Chem Inf Model 52(8):2098\u20132106. https:\/\/doi.org\/10.1021\/ci200612f","journal-title":"J Chem Inf Model"},{"issue":"5","key":"1812_CR29","doi-asserted-by":"publisher","first-page":"2083","DOI":"10.1021\/acs.jmedchem.5b01723","volume":"59","author":"VO Talibov","year":"2016","unstructured":"Talibov VO, Linkuvien\u0117 V, Matulis D, Danielson UH (2016) Kinetically selective inhibitors of human carbonic anhydrase isozymes I, II, VII, IX, XII, and XIII. J Med Chem 59(5):2083\u20132093. https:\/\/doi.org\/10.1021\/acs.jmedchem.5b01723","journal-title":"J Med Chem"},{"key":"1812_CR24","doi-asserted-by":"publisher","unstructured":"Va\u0161kevi\u010dius A, Baronas D, Leitans J, Kvietkauskait\u0117 A, Ruk\u0161\u0117nait\u0117 A, Manakova E, Toleikis Z, Kaupinis A, Kazaks A, Gedgaudas M, Mickevi\u010di\u016bt\u0117 A, Juozapaitien\u0117 V, Schi\u00f6th HB, Jaudzems K, Valius M, Tars K, Gra\u017eulis S, Meyer-Almes F-J, Matulien\u0117 J, Zubrien\u0117 A, Dudutien\u0117 V, Matulis D (2024) Targeted Anticancer Pre-Vinylsulfone Covalent Inhibitors of Carbonic Anhydrase IX. eLife 13:RP101401. https:\/\/doi.org\/10.7554\/eLife.101401","DOI":"10.7554\/eLife.101401"},{"key":"1812_CR14","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.5c01142","author":"A Va\u0161kevi\u010dius","year":"2025","unstructured":"Va\u0161kevi\u010dius A, \u017dvirblis M, Kurtenoka M, Leitans J, Manakova E, Paketuryt\u0117-Latv\u0117 V, Kvietkauskait\u0117 A, Kazaks A, Eimonta V, \u010cerepenkait\u0117 K, Kazokait\u0117-Adomaitien\u0117 J, Mickevi\u010diu\u0305t\u0117 A, Juozapaitien\u0117 V, Tars K, Gra\u017eulis S, Matulien\u0117 J, Dudutien\u0117 V, Shubin K, Matulis D, Zubrien\u0117 A (2025) Di-Meta-Substituted fluorinated benzenesulfonamides as potent and selective anticancer inhibitors of carbonic anhydrase IX and XII. J Med Chem. https:\/\/doi.org\/10.1021\/acs.jmedchem.5c01142","journal-title":"J Med Chem"},{"key":"1812_CR18","doi-asserted-by":"publisher","unstructured":"Zubrien\u0117 A, Matulis D (2019) Observed Versus Intrinsic Thermodynamics of Inhibitor Binding to Carbonic Anhydrases. In: Matulis D (ed) Carbonic Anhydrase as Drug Target: Thermodynamics and Structure of Inhibitor Binding, Springer International Publishing: Cham, pp 107\u2013123. https:\/\/doi.org\/10.1007\/978-3-030-12780-0_8","DOI":"10.1007\/978-3-030-12780-0_8"}],"container-title":["European Biophysics Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00249-025-01812-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00249-025-01812-5","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00249-025-01812-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T18:03:05Z","timestamp":1771869785000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00249-025-01812-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,19]]},"references-count":31,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["1812"],"URL":"https:\/\/doi.org\/10.1007\/s00249-025-01812-5","relation":{},"ISSN":["0175-7571","1432-1017"],"issn-type":[{"value":"0175-7571","type":"print"},{"value":"1432-1017","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,19]]},"assertion":[{"value":"2 October 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 December 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 January 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Authors declare that they have patent applications pending and patents on CA inhibitors.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}