{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T04:11:05Z","timestamp":1789618265101,"version":"build-2803163510"},"reference-count":62,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T00:00:00Z","timestamp":1669939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T00:00:00Z","timestamp":1669939200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Immunogenetics"],"published-print":{"date-parts":[[2023,4]]},"DOI":"10.1007\/s00251-022-01282-5","type":"journal-article","created":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T12:03:35Z","timestamp":1669982615000},"page":"99-114","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Bioinformatics analysis of structural protein to approach a vaccine candidate against Vibrio cholerae infection"],"prefix":"10.1007","volume":"75","author":[{"given":"Elijah Kolawole","family":"Oladipo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olawumi Elizabeth","family":"Akindiya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glory Jesudara","family":"Oluwasanya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gideon Mayowa","family":"Akanbi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seun Elijah","family":"Olufemi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Adewole","family":"Adediran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Favour Oluwadara","family":"Bamigboye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasidat Oyindamola","family":"Aremu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kehinde Temitope","family":"Kolapo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerry Ayobami","family":"Oluwasegun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hezekiah Oluwajoba","family":"Awobiyi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther Moradeyo","family":"Jimah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boluwatife Ayobami","family":"Irewolede","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth Oluwatoyin","family":"Folakanmi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Odunola Abimbola","family":"Olubodun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel Adebowale","family":"Akintibubo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Foluso Daniel","family":"Odunlami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taiwo Ooreoluwa","family":"Ojo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omodamola Paulina","family":"Akinro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oluwaseun Samuel","family":"Hezikiah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adenike Titilayo","family":"Olayinka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace Asegunloluwa","family":"Abiala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akindele Felix","family":"Idowu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James Akinwunmi","family":"Ogunniran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary Omotoyinbo","family":"Ikuomola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hadijat Motunrayo","family":"Adegoke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Usman Abiodun","family":"Idowu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oluwaseyi Paul","family":"Olaniyan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olutoyin Omolara","family":"Bamigboye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunday Babatunde","family":"Akinde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Musa Oladayo","family":"Babalola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,12,2]]},"reference":[{"issue":"10","key":"1282_CR1","doi-asserted-by":"publisher","first-page":"3793","DOI":"10.1080\/07391102.2020.1770127","volume":"39","author":"K Abraham Peele","year":"2021","unstructured":"Abraham Peele K, Srihansa T, Krupanidhi S, Ayyagari VS, Venkateswarulu TC (2021) Design of multi-epitope vaccine candidate against SARS-CoV-2: a in-silico study. J Biomol Struct Dyn 39(10):3793\u20133801","journal-title":"J Biomol Struct Dyn"},{"issue":"1","key":"1282_CR2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s40794-021-00147-1","volume":"7","author":"KM Adam","year":"2021","unstructured":"Adam KM (2021) Immunoinformatics approach for multi-epitope vaccine design against structural proteins and ORF1a polyprotein of severe acute respiratory syndrome coronavirus-2 (SARS-CoV- 2). Tropical Diseases, Travel Medicine and Vaccines 7(1):1\u201313","journal-title":"Tropical Diseases, Travel Medicine and Vaccines"},{"issue":"1\u20134","key":"1282_CR3","first-page":"14","volume":"85","author":"W Adero","year":"2021","unstructured":"Adero W, Nyanjom S, Mwirigi MK, Yattinder B (2021) In-silico epitope prediction of small ruminant morbillivirus proteins as potential vaccine candidates. East African Agricultural and Forestry Journal 85(1\u20134):14\u201314","journal-title":"East African Agricultural and Forestry Journal"},{"key":"1282_CR4","doi-asserted-by":"crossref","unstructured":"Adhikari UK, Tayebi M, Rahman MM (2018) Immunoinformatics approach for epitope-based peptide vaccine design and active site prediction against polyprotein of emerging oropouche virus.\u00a0J Immunol Res\u00a02018","DOI":"10.1155\/2018\/6718083"},{"issue":"6","key":"1282_CR5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003832","volume":"9","author":"M Ali","year":"2015","unstructured":"Ali M, Nelson AR, Lopez AL, Sack D (2015) PLoS Negl Trop Dis 9(6):e0003832. https:\/\/doi.org\/10.1371\/journal.pntd.0003832","journal-title":"PLoS Negl Trop Dis"},{"key":"1282_CR6","doi-asserted-by":"crossref","unstructured":"Banerjee S, Majumder K, Gutierrez GJ, Gupta D, Mittal B (2020) Immuno-informatics approach for multi-epitope vaccine designing against SARS-CoV-2.\u00a0BioRxiv","DOI":"10.1101\/2020.07.23.218529"},{"key":"1282_CR7","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1186\/1471-2105-7-153","volume":"7","author":"HH Bui","year":"2006","unstructured":"Bui HH, Sidney J, Dinh K, Southwood S, Newman MJ, Sette A (2006) Predicting population coverage of T-cell epitope-based diagnostics and vaccines. BMC Bioinformatics 7:153. https:\/\/doi.org\/10.1186\/1471-2105-7-153","journal-title":"BMC Bioinformatics"},{"key":"1282_CR8","doi-asserted-by":"publisher","unstructured":"CDC (2020) Cholera: Vibrio cholerae infection. Centers for Disease Control and Prevention. Cole Supe\u00b4rieure de Biotechnologie de Strasbourg, Boulevard Se\u00b4bastien Brandt, 67400 Illkirch, France. Biologicals (2001) 29:209\u2013213.\u00a0https:\/\/doi.org\/10.1006\/biol.2001.0308, http:\/\/www.idealibrary.com","DOI":"10.1006\/biol.2001.0308"},{"issue":"3","key":"1282_CR9","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1007\/s00726-006-0485-9","volume":"33","author":"J Chen","year":"2007","unstructured":"Chen J, Liu H, Yang J, Chou KC (2007) Prediction of linear B-cell epitopes using amino acid pair antigenicity scale. Amino Acids 33(3):423\u2013428. https:\/\/doi.org\/10.1007\/s00726-006-0485-9","journal-title":"Amino Acids"},{"key":"1282_CR10","doi-asserted-by":"crossref","unstructured":"Coscolla M, Copin R, Sutherland J, Gehre F, de Jong B, Owolabi O, Gagneux SM (2015) Tuberculosis T cell epitope analysis reveals paucity of antigenic variation and identifies rare variable TB antigens.\u00a0Cell Host Microbe\u00a018(5):538\u2013548","DOI":"10.1016\/j.chom.2015.10.008"},{"issue":"5","key":"1282_CR11","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1586\/14760584.2014.907528","volume":"13","author":"AK Dey","year":"2014","unstructured":"Dey AK, Malyala P, Singh M (2014) Physicochemical and functional characterization of vaccine antigens and adjuvants. Expert Rev Vaccines 13(5):671\u2013685","journal-title":"Expert Rev Vaccines"},{"key":"1282_CR12","doi-asserted-by":"publisher","first-page":"1784","DOI":"10.3389\/fimmu.2020.01784","volume":"11","author":"R Dong","year":"2020","unstructured":"Dong R, Chu Z, Yu F, Zha Y (2020) Contriving multi-epitope subunit of vaccine for COVID-19: immunoinformatics approaches. Front Immunol 11:1784","journal-title":"Front Immunol"},{"issue":"1","key":"1282_CR13","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1471-2105-8-4","volume":"8","author":"IA Doytchinova","year":"2007","unstructured":"Doytchinova IA, Flower DR (2007) VaxiJen: a server for prediction of protective antigens, tumour antigens and subunit vaccines. BMC Bioinformatics 8(1):1\u20137","journal-title":"BMC Bioinformatics"},{"key":"1282_CR14","unstructured":"Dunkin MA (2021) Cholera. WebMD. https:\/\/www.webmd.com\/a-to-z-guides\/cholera-faq"},{"key":"1282_CR15","doi-asserted-by":"publisher","DOI":"10.1016\/j.imu.2021.100764","volume":"26","author":"LO Ezediuno","year":"2021","unstructured":"Ezediuno LO, Onile OS, Oladipo EK, Majolagbe ON, Jimah EM, Senbadejo TY (2021) Designing multi-epitope subunit vaccine for ocular trachoma infection using Chlamydia trachomatis polymorphic membrane proteins G. Informatics in Medicine Unlocked 26:100764","journal-title":"Informatics in Medicine Unlocked"},{"issue":"3","key":"1282_CR16","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1007\/s10989-015-9461-0","volume":"21","author":"T Farhadi","year":"2015","unstructured":"Farhadi T, Nezafat N, Ghasemi Y, Karimi Z, Hemmati S, Erfani N (2015) Designing of complex multi-epitope peptide vaccine based on omps of Klebsiella pneumoniae: an in-silico approach. Int J Pept Res Ther 21(3):325\u2013341","journal-title":"Int J Pept Res Ther"},{"key":"1282_CR17","doi-asserted-by":"publisher","unstructured":"Flower DR, Macdonald IK, Ramakrishnan K, Davies MN, Doytchinova IA (2010) Computer aided selection of candidate vaccine antigens.\u00a0Immunome Research\u00a06(Suppl 2):S1. https:\/\/doi.org\/10.1186\/1745-7580-6-S2-S1","DOI":"10.1186\/1745-7580-6-S2-S1"},{"key":"1282_CR18","doi-asserted-by":"crossref","unstructured":"Gasteiger E, Hoogland C, Gattiker A, Duvaud S, Wilkins MR, Appel RD, Bairoch A (2005) In John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press pp.571\u2013607 Full text\u00a0- Copyright Humana Press","DOI":"10.1385\/1-59259-890-0:571"},{"issue":"3","key":"1282_CR19","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s10989-022-10406-0","volume":"28","author":"M Ghandadi","year":"2022","unstructured":"Ghandadi M (2022) An Immunoinformatic strategy to develop new Mycobacterium tuberculosis multi-epitope vaccine. Int J Pept Res Ther 28(3):1\u201314","journal-title":"Int J Pept Res Ther"},{"issue":"1645","key":"1282_CR20","doi-asserted-by":"publisher","first-page":"20130433","DOI":"10.1098\/rstb.2013.0433","volume":"369","author":"B Greenwood","year":"2014","unstructured":"Greenwood B (2014) The contribution of vaccination to global health: past, present and future. Philosophical Transactions of the Royal Society B: Biological Sciences 369(1645):20130433","journal-title":"Philosophical Transactions of the Royal Society B: Biological Sciences"},{"key":"1282_CR21","doi-asserted-by":"publisher","unstructured":"Grote A, Hiller K, Scheer M, M\u00fcnch R, N\u00f6rtemann B, Hempel DC, Jahn D (2005) JCat: a novel tool to adapt codon usage of a target gene to its potential expression host.\u00a0Nucleic Acids Res 33(Web Server issue):W526\u2013W531. https:\/\/doi.org\/10.1093\/nar\/gki376","DOI":"10.1093\/nar\/gki376"},{"key":"1282_CR22","doi-asserted-by":"publisher","unstructured":"Gupta S, Kapoor P, Chaudhary K, Gautam A, Kumar R, Open Source Drug Discovery Consortium and Raghava GP (2013) In silico approach for predicting toxicity of peptides and proteins. PLoS One 8(9):e73957. https:\/\/doi.org\/10.1371\/journal.pone.0073957","DOI":"10.1371\/journal.pone.0073957"},{"key":"1282_CR23","doi-asserted-by":"crossref","unstructured":"Habib PT (2020) Learning from COVID-19 Pandemic: In Silico Vaccine and Cloning Design Against Nipah Virus by Studying and Analyzing the Whole Nipah Virus Proteome","DOI":"10.21203\/rs.3.rs-269666\/v1"},{"key":"1282_CR24","doi-asserted-by":"crossref","unstructured":"Helen HM, Shoubai C (2020) Advances in Vaccines. Current Application of Pharmaceutical Biotechnology 171:155\u2013188.\u00a0https:\/\/www.cdc.gov\/cholera\/infection-sources.html","DOI":"10.1007\/10_2019_107"},{"issue":"2","key":"1282_CR25","doi-asserted-by":"publisher","first-page":"64","DOI":"10.3390\/tropicalmed6020064","volume":"6","author":"J Holmgren","year":"2021","unstructured":"Holmgren J (2021) An update on cholera immunity and current and future cholera vaccines. Tropical Medicine and Infectious Disease 6(2):64","journal-title":"Tropical Medicine and Infectious Disease"},{"issue":"2","key":"1282_CR26","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1006\/jmbi.1999.3091","volume":"292","author":"DT Jones","year":"1999","unstructured":"Jones DT (1999) Protein secondary structure prediction based on position-specific scoring matrices. J Mol Biol 292(2):195\u2013202. https:\/\/doi.org\/10.1006\/jmbi.1999.3091","journal-title":"J Mol Biol"},{"key":"1282_CR27","doi-asserted-by":"crossref","unstructured":"Jyotisha SS, Qureshi IA (2022) Multi-epitope vaccine against SARS-CoV-2 applying immunoinformatics and molecular dynamics simulation approaches. J Biomol Struct Dyn 40(7):2917\u20132933","DOI":"10.1080\/07391102.2020.1844060"},{"issue":"1","key":"1282_CR28","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-019-49354-z","volume":"9","author":"M Khan","year":"2019","unstructured":"Khan M, Khan S, Ali A, Akbar H, Sayaf AM, Khan A, Wei DQ (2019) Immunoinformatics approaches to explore Helicobacter Pylori proteome (Virulence Factors) to design B and T cell multi-epitope subunit vaccine. Sci Rep 9(1):1\u201313","journal-title":"Sci Rep"},{"issue":"2","key":"1282_CR29","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1038\/nprot.2016.169","volume":"12","author":"D Kozakov","year":"2017","unstructured":"Kozakov D, Hall DR, Xia B, Porter KA, Padhorny D, Yueh C, Vajda S (2017) The ClusPro web server for protein\u2013protein docking. Nat Protoc 12(2):255\u2013278","journal-title":"Nat Protoc"},{"issue":"1","key":"1282_CR30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1471-2105-8-424","volume":"8","author":"MV Larsen","year":"2007","unstructured":"Larsen MV, Lundegaard C, Lamberth K, Buus S, Lund O, Nielsen M (2007) Large-scale validation of methods for cytotoxic T-lymphocyte epitope prediction. BMC Bioinformatics 8(1):1\u201312","journal-title":"BMC Bioinformatics"},{"issue":"4","key":"1282_CR31","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1038\/s41577-021-00583-2","volume":"22","author":"EC Lavelle","year":"2022","unstructured":"Lavelle EC, Ward RW (2022) Mucosal vaccines\u2014fortifying the frontiers. Nat Rev Immunol 22(4):236\u2013250","journal-title":"Nat Rev Immunol"},{"issue":"11","key":"1282_CR32","doi-asserted-by":"publisher","first-page":"5499","DOI":"10.4049\/jimmunol.168.11.5499","volume":"168","author":"B Livingston","year":"2002","unstructured":"Livingston B, Crimi C, Newman M, Higashimoto Y, Appella E, Sidney J, Sette A (2002) A rational strategy to design multiepitope immunogens based on multiple Th lymphocyte epitopes. J Immunol 168(11):5499\u20135506","journal-title":"J Immunol"},{"key":"1282_CR33","first-page":"3","volume":"2","author":"RR Mar\u00eda","year":"2017","unstructured":"Mar\u00eda RR, Arturo CJ, Alicia JA, Paulina MG, Gerardo AO (2017) The impact of bioinformatics on vaccine design and development. Vaccines 2:3\u20136","journal-title":"Vaccines"},{"issue":"8","key":"1282_CR34","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1093\/protein\/14.8.529","volume":"14","author":"T Nagamune","year":"2001","unstructured":"Nagamune T (2001) Design of the linkers which effectively separate domains of a bifunctional fusion protein. Protein Eng Des Sel 14(8):529\u2013532. https:\/\/doi.org\/10.1093\/protein\/14.8.529","journal-title":"Protein Eng Des Sel"},{"key":"1282_CR35","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.compbiolchem.2016.04.006","volume":"62","author":"N Nezafat","year":"2016","unstructured":"Nezafat N, Karimi Z, Eslami M, Mohkam M, Zandian S, Ghasemi Y (2016) Designing an efficient multi-epitope peptide vaccine against Vibrio cholerae via combined immunoinformatics and protein interaction-based approaches. Comput Biol Chem 62:82\u201395","journal-title":"Comput Biol Chem"},{"key":"1282_CR36","doi-asserted-by":"publisher","unstructured":"Nielsen M, Lund O, Buus S, Lundegaard C (2010) MHC class II epitope predictive algorithms. Immunology 130(3):319\u201328.\u00a0https:\/\/doi.org\/10.1111\/j.1365-2567.2010.03268.x. Epub 2010 Apr 12. PMID: 20408898; PMCID: PMC2913211","DOI":"10.1111\/j.1365-2567.2010.03268.x"},{"key":"1282_CR37","doi-asserted-by":"crossref","unstructured":"Nielsen M, Andreatta M, Peters B, Buus S (2020) Immunoinformatics: predicting peptide\u2013MHC binding","DOI":"10.1146\/annurev-biodatasci-021920-100259"},{"key":"1282_CR38","unstructured":"NORD (2021) Rare disease https:\/\/rarediseases.org\/rare-diseases\/cholera\/"},{"key":"1282_CR39","doi-asserted-by":"publisher","unstructured":"Oladipo EK, Jimah EM, Irewolede BA, Folakanmi EO, Olubodun OA, Adediran DA, Akintibubo SA, Odunlami FD, Olufemi SE, Ojo TO, Akinro OP, Hezekiah OS, Olayinka AT, Abiala GA, Idowu AF, Ogunniran JA, Ikuomola MO, Adegoke HM, Idowu UA, Akindiya OE, Adelusi TI (2022) Immunoinformatics design of multi-epitope peptide for the diagnosis of\u00a0Schistosoma haematobium\u00a0infection.\u00a0J Biomol Struct Dyn\u00a01\u20138. Advance online publication. https:\/\/doi.org\/10.1080\/07391102.2022.2111358","DOI":"10.1080\/07391102.2022.2111358"},{"key":"1282_CR40","doi-asserted-by":"publisher","first-page":"13","DOI":"10.2147\/ITT.S241064","volume":"9","author":"AN Oli","year":"2020","unstructured":"Oli AN, Obialor WO, Ifeanyichukwu MO, Odimegwu DC, Okoyeh JN, Emechebe GO, Ibeanu GC (2020) Immunoinformatics and vaccine development: an overview. ImmunoTargets and Therapy 9:13","journal-title":"ImmunoTargets and Therapy"},{"issue":"6","key":"1282_CR41","doi-asserted-by":"publisher","first-page":"1034","DOI":"10.1016\/j.drudis.2020.03.006","volume":"25","author":"S Parvizpour","year":"2020","unstructured":"Parvizpour S, Pourseif MM, Razmara J, Rafi MA, Omidi Y (2020) Epitope-based vaccine design: a comprehensive overview of bioinformatics approaches. Drug Discov Today 25(6):1034\u20131042","journal-title":"Drug Discov Today"},{"key":"1282_CR42","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1016\/j.coviro.2016.03.003","volume":"17","author":"GA Poland","year":"2016","unstructured":"Poland GA, Whitaker JA, Poland CM, Ovsyannikova IG, Kennedy RB (2016) Vaccinology in the third millennium: scientific and social challenges. Curr Opin Virol 17:116\u2013125. https:\/\/doi.org\/10.1016\/j.coviro.2016.03.003","journal-title":"Curr Opin Virol"},{"issue":"4","key":"1282_CR43","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009862","volume":"5","author":"N Rapin","year":"2010","unstructured":"Rapin N, Lund O, Bernaschi M, Castiglione F (2010) Computational immunology meets bioinformatics: the use of prediction tools for molecular binding in the simulation of the immune system. PLoS ONE 5(4):e9862","journal-title":"PLoS ONE"},{"issue":"1","key":"1282_CR44","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1002\/prot.21078","volume":"65","author":"S Saha","year":"2006","unstructured":"Saha S, Raghava GP (2006) Prediction of continuous B-cell epitopes in an antigen using recurrent neural network. Proteins 65(1):40\u201348. https:\/\/doi.org\/10.1002\/prot.21078","journal-title":"Proteins"},{"key":"1282_CR45","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmgm.2019.107477","volume":"94","author":"R Sajjad","year":"2020","unstructured":"Sajjad R, Ahmad S, Azam SS (2020) In silico screening of antigenic B-cell derived T-cell epitopes and designing of a multi-epitope peptide vaccine for Acinetobacter nosocomialis. J Mol Graph Model 94:107477","journal-title":"J Mol Graph Model"},{"issue":"1","key":"1282_CR46","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-021-91997-4","volume":"11","author":"S Sanami","year":"2021","unstructured":"Sanami S, Azadegan-Dehkordi F, Rafieian-Kopaei M, Salehi M, Ghasemi-Dehnoo M, Mahooti M, Bagheri N (2021) Design of a multi-epitope vaccine against cervical cancer using immunoinformatics approaches. Sci Rep 11(1):1\u201315","journal-title":"Sci Rep"},{"issue":"1","key":"1282_CR47","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s43141-022-00379-4","volume":"20","author":"M Shahab","year":"2022","unstructured":"Shahab M, Hayat C, Sikandar R, Zheng G, Akter S (2022) In silico designing of a multi-epitope vaccine against Burkholderia pseudomallei: reverse vaccinology and immunoinformatics. J Genet Eng Biotechnol 20(1):1\u201312","journal-title":"J Genet Eng Biotechnol"},{"key":"1282_CR48","doi-asserted-by":"publisher","unstructured":"Sharma N, Patiyal S, Dhall A, Pande A, Arora C, Raghava GPS (2021) AlgPred 2.0: an improved method for predicting allergenic proteins and mapping of IgE epitopes. Brief Bioinform 22(4):bbaa294.\u00a0https:\/\/doi.org\/10.1093\/bib\/bbaa294. PMID: 33201237","DOI":"10.1093\/bib\/bbaa294"},{"issue":"12","key":"1282_CR49","doi-asserted-by":"publisher","first-page":"405","DOI":"10.6026\/97320630013405","volume":"13","author":"C Somboonwit","year":"2017","unstructured":"Somboonwit C, Menezes LJ, Holt DA, Sinnott JT, Shapshak P (2017) Current views and challenges on clinical cholera. Bioinformation 13(12):405","journal-title":"Bioinformation"},{"key":"1282_CR50","doi-asserted-by":"publisher","unstructured":"Stratmann T (2015) Cholera Toxin Subunit B as Adjuvant--An Accelerator in Protective Immunity and a Break in Autoimmunity. Vaccines (Basel) 3(3):579\u201396.\u00a0https:\/\/doi.org\/10.3390\/vaccines3030579. PMID: 26350596; PMCID: PMC4586468","DOI":"10.3390\/vaccines3030579"},{"issue":"6","key":"1282_CR51","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1007\/s00251-010-0441-4","volume":"62","author":"T Stranzl","year":"2010","unstructured":"Stranzl T, Larsen MV, Lundegaard C, Nielsen M (2010) NetCTLpan: pan-specific MHC class I pathway epitope predictions. Immunogenetics 62(6):357\u2013368. https:\/\/doi.org\/10.1007\/s00251-010-0441-4","journal-title":"Immunogenetics"},{"key":"1282_CR52","doi-asserted-by":"crossref","unstructured":"ul Qamar MT, Ahmad S, Fatima I, Ahmad F, Shahid F, Naz A, Chen LL (2021) Designing multi-epitope vaccine against Staphylococcus aureus by employing subtractive proteomics, reverse vaccinology and immuno-informatics approaches.\u00a0Comput Biol Med\u00a0132:104389","DOI":"10.1016\/j.compbiomed.2021.104389"},{"key":"1282_CR53","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1016\/j.molimm.2018.11.005","volume":"104","author":"M Validi","year":"2018","unstructured":"Validi M, Karkhah A, Prajapati VK, Nouri HR (2018) Immuno-informatics based approaches to design a novel multi epitope-based vaccine for immune response reinforcement against Leptospirosis. Mol Immunol 104:128\u2013138","journal-title":"Mol Immunol"},{"issue":"D1","key":"1282_CR54","doi-asserted-by":"publisher","first-page":"D339","DOI":"10.1093\/nar\/gky1006","volume":"47","author":"Randi Vita","year":"2019","unstructured":"Vita R, Mahajan S, Overton JA, Dhanda SK, Martini S, Cantrell JR, Wheeler DK, Sette A, Peters B (2019) The immune epitope database (IEDB): 2018 update. Nucleic Acids Res 47(D1):D339\u2013D343. https:\/\/doi.org\/10.1093\/nar\/gky1006","journal-title":"Nucleic Acids Research"},{"key":"1282_CR55","doi-asserted-by":"crossref","unstructured":"Vita R, Overton JA, Greenbaum JA et al\u00a0(2015) The immune epitope database (IEDB) 3.0. Nucleic 449 acids research. 43:D405-D412","DOI":"10.1093\/nar\/gku938"},{"issue":"1","key":"1282_CR56","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1128\/iai.62.1.72-78.1994","volume":"62","author":"MK Waldor","year":"1994","unstructured":"Waldor MK, Mekalanos JJ (1994) ToxR regulates virulence gene expression in non-O1 strains of Vibrio cholerae that cause epidemic cholera. Infect Immun 62(1):72\u201378","journal-title":"Infect Immun"},{"issue":"1","key":"1282_CR57","doi-asserted-by":"publisher","first-page":"386","DOI":"10.1080\/10717544.2022.2030430","volume":"29","author":"L Wang","year":"2022","unstructured":"Wang L, Geng J, Chen L, Guo X, Wang T, Fang Y, Belingon B, Jiao Wu, Li M, Zhan Y, Shang W, Wan Y, Feng X, Xianghui Li Hu, Wang (2022) Improved transfer efficiency of supercharged 36 + GFP protein mediate nucleic acid delivery. Drug Deliv 29(1):386\u2013398","journal-title":"Drug Deliv"},{"key":"1282_CR58","doi-asserted-by":"publisher","first-page":"292","DOI":"10.3389\/fimmu.2017.00292","volume":"8","author":"M Wieczorek","year":"2017","unstructured":"Wieczorek M, Abualrous ET, Sticht J, \u00c1lvaro-Benito M, Stolzenberg S, No\u00e9 F, Freund C (2017) Major histocompatibility complex (MHC) class I and MHC class II proteins: conformational plasticity in antigen presentation. Front Immunol 8:292","journal-title":"Front Immunol"},{"key":"1282_CR59","unstructured":"World Health Organization (2022) Disease Outbreak News; Cholera \u2013 Malawi. Available at: https:\/\/www.who.int\/emergencies\/disease-outbreak-news\/item\/2022-DON419"},{"issue":"1","key":"1282_CR60","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1002\/0471250953.bi0508s52","volume":"52","author":"J Yang","year":"2015","unstructured":"Yang J, Zhang Y (2015) Protein structure and function prediction using I-TASSER. Curr Protoc Bioinformatics 52(1):5\u20138","journal-title":"Curr Protoc Bioinformatics"},{"key":"1282_CR61","first-page":"298","volume":"10","author":"N Zaharieva","year":"2017","unstructured":"Zaharieva N, Dimitrov I, Flower D, Doytchinova I (2017) Immunogenicity prediction by VaxiJen: a ten-year overview. J Proteomics Bioinformatics 10:298\u2013310","journal-title":"J Proteomics Bioinformatics"},{"issue":"10","key":"1282_CR62","doi-asserted-by":"publisher","first-page":"2326","DOI":"10.1038\/s41596-022-00728-0","volume":"17","author":"Xiaogen Zhou","year":"2022","unstructured":"Zhou X, Zheng W, Li Y, Pearce R, Zhang C, Bell, EW, Zhang G, Zhang Y (2022) I-TASSER-MTD: a deep-learning-based platform for multi-domain protein structure and function prediction. Nat Protoc 17(10):2326\u20132353. https:\/\/doi.org\/10.1038\/s41596-022-00728-0","journal-title":"Nature Protocols"}],"container-title":["Immunogenetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00251-022-01282-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00251-022-01282-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00251-022-01282-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T13:48:35Z","timestamp":1744206515000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00251-022-01282-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,2]]},"references-count":62,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,4]]}},"alternative-id":["1282"],"URL":"https:\/\/doi.org\/10.1007\/s00251-022-01282-5","relation":{},"ISSN":["0093-7711","1432-1211"],"issn-type":[{"value":"0093-7711","type":"print"},{"value":"1432-1211","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,2]]},"assertion":[{"value":"25 August 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 October 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 December 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing of interest"}}]}}