{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:56:44Z","timestamp":1790776604029,"version":"4.1.0"},"reference-count":45,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2007,2,1]],"date-time":"2007-02-01T00:00:00Z","timestamp":1170288000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"published-print":{"date-parts":[[2007,2]]},"DOI":"10.1007\/s00253-006-0763-1","type":"journal-article","created":{"date-parts":[[2007,1,10]],"date-time":"2007-01-10T11:36:38Z","timestamp":1168428998000},"page":"282-289","source":"Crossref","is-referenced-by-count":34,"title":["Thymidyl biosynthesis enzymes as antibiotic targets"],"prefix":"10.1007","volume":"74","author":[{"given":"Anatoly","family":"Chernyshev","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd","family":"Fleischmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amnon","family":"Kohen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2007,2,1]]},"reference":[{"key":"763_CR1","doi-asserted-by":"crossref","first-page":"10295","DOI":"10.1021\/bi0490439","volume":"43","author":"N Agrawal","year":"2004","unstructured":"Agrawal N, Lesley SA, Kuhn P, Kohen A (2004) Mechanistic studies of a flavin-dependent thymidylate synthase. Biochemistry 43:10295\u201310301","journal-title":"Biochemistry"},{"key":"763_CR2","doi-asserted-by":"crossref","first-page":"18314","DOI":"10.1074\/jbc.M400009200","volume":"279","author":"CE Atreya","year":"2004","unstructured":"Atreya CE, Anderson KS (2004) Kinetic characterization of bifunctional thymidylate synthase-dihydrofolate reductase (TS-DHFR) from Cryptosporidium hominis: a paradigm shift for ts activity and channeling behavior. J Biol Chem 279:18314\u201318322","journal-title":"J Biol Chem"},{"key":"763_CR3","doi-asserted-by":"crossref","first-page":"14092","DOI":"10.1074\/jbc.M212690200","volume":"278","author":"CE Atreya","year":"2003","unstructured":"Atreya CE, Johnson EF, Irwin JJ, Dow A, Massimine KM, Coppens I, Stempliuk V, Beverley S, Joiner KA, Shoichet BK, Anderson KS (2003) A molecular docking strategy identifies Eosin B as a non-active site inhibitor of protozoal bifunctional thymidylate synthase-dihydrofolate reductase. J Biol Chem 278:14092\u201314100","journal-title":"J Biol Chem"},{"key":"763_CR4","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1021\/ja973210t","volume":"120","author":"JE Barrett","year":"1998","unstructured":"Barrett JE, Maltby DA, Santi DV, Schultz PG (1998) Trapping of the C5 methylene intermediate in thymidylate synthase. J Am Chem Soc 120:449\u2013450","journal-title":"J Am Chem Soc"},{"key":"763_CR5","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1146\/annurev.bi.64.070195.003445","volume":"64","author":"CW Carreras","year":"1995","unstructured":"Carreras CW, Santi DV (1995) The catalytic mechanism and structure of thymidylate synthase. Annu Rev Biochem 64:721\u2013762","journal-title":"Annu Rev Biochem"},{"key":"763_CR6","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1073\/pnas.90.2.517","volume":"90","author":"E Chu","year":"1993","unstructured":"Chu E, Voeller D, Koeller DM, Drake JC, Takimoto CH, Maley GF, Maley F, Allegra CJ (1993) Identification of an RNA binding site for human thymidylate synthase. Proc Natl Acad Sci USA 90:517\u2013521","journal-title":"Proc Natl Acad Sci USA"},{"key":"763_CR7","first-page":"621","volume":"26","author":"PV Danenberg","year":"1999","unstructured":"Danenberg PV, Malli H, Swenson S (1999) Thymidylate synthase inhibitors. Semin Oncol 26:621\u2013631","journal-title":"Semin Oncol"},{"key":"763_CR8","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1073\/pnas.72.2.528","volume":"72","author":"AS Delk","year":"1975","unstructured":"Delk AS, Rabinowitz JC (1975) Biosynthesis of ribosylthymine in the transfer RNA of Streptococcus faecalis: a folate-dependent methylation not involving S-adenosylmethionine. Proc Natl Acad Sci USA 72:528\u2013530","journal-title":"Proc Natl Acad Sci USA"},{"key":"763_CR9","doi-asserted-by":"crossref","first-page":"7649","DOI":"10.1016\/S0021-9258(17)32901-0","volume":"251","author":"AS Delk","year":"1976","unstructured":"Delk AS, Romeo JM, Nagle DP Jr, Rabinowitz JC (1976) Biosynthesis of ribothymidine in the transfer RNA of Streptococcus faecalis and Bacillus subtilis. A methylation of RNA involving 5,10-methylenetetrahydrofolate. J Biol Chem 251:7649\u20137656","journal-title":"J Biol Chem"},{"key":"763_CR10","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/0006-291X(79)90858-1","volume":"86","author":"AS Delk","year":"1979","unstructured":"Delk AS, Nagle DP Jr, Rabinowitz JC, Straub KM (1979) The methylenetetrahydrofolate-mediated biosynthesis of ribothymidine in the transfer-RNA of Streptococcus faecalis: incorporation of hydrogen from solvent into the methyl moiety. Biochem Biophys Res Commun 86:244\u2013251","journal-title":"Biochem Biophys Res Commun"},{"key":"763_CR11","doi-asserted-by":"crossref","first-page":"4387","DOI":"10.1016\/S0021-9258(19)85498-4","volume":"255","author":"AS Delk","year":"1980","unstructured":"Delk AS, Nagle DP Jr, Rabinowitz JC (1980) Methylenetetrahydrofolate-dependent biosynthesis of ribothymidine in transfer RNA of Streptococcus faecalis. Evidence for reduction of the 1-carbon unit by FADH2. J Biol Chem 255:4387\u20134390","journal-title":"J Biol Chem"},{"key":"763_CR12","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1021\/ja0432214","volume":"127","author":"SG Gattis","year":"2005","unstructured":"Gattis SG, Palfey BA (2005) Direct observation of the participation of flavin in product formation by thyX-encoded thymidylate synthase. J Am Chem Soc 127:832\u2013833","journal-title":"J Am Chem Soc"},{"key":"763_CR13","doi-asserted-by":"crossref","first-page":"54340","DOI":"10.1074\/jbc.M409121200","volume":"279","author":"S Graziani","year":"2004","unstructured":"Graziani S et al. (2004) Functional analysis of FAD-dependent thymidylate synthase ThyX from paramecium bursaria chlorella virus-1. J Biol Chem 279:54340\u201354347","journal-title":"J Biol Chem"},{"issue":"33","key":"763_CR14","doi-asserted-by":"crossref","first-page":"24048","DOI":"10.1074\/jbc.M600745200","volume":"281","author":"S Graziani","year":"2006","unstructured":"Graziani S, Bernauer J, Skouloubris S, Graille M, Zhou CZ, Marchand C, Decottignies P, van Tilbeurgh H, Myllykallio H, Liebl U (2006) Catalytic mechanism and structure of viral flavin-dependent thymidylate synthase ThyX. J Biol Chem 281(33):24048\u201324057","journal-title":"J Biol Chem"},{"key":"763_CR15","doi-asserted-by":"crossref","first-page":"5456","DOI":"10.1074\/jbc.M412415200","volume":"280","author":"J Griffin","year":"2005","unstructured":"Griffin J, Roshick C, Iliffe-Lee E, McClarty G (2005) Catalytic mechanism of Chlamydia trachomatis flavin-dependent thymidylate synthase. J Biol Chem 280:5456\u20135467","journal-title":"J Biol Chem"},{"key":"763_CR16","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1096\/fasebj.4.6.2180768","volume":"4","author":"KM Ivanetich","year":"1990","unstructured":"Ivanetich KM, Santi DV (1990) Bifunctional thymidylate synthase-dihydrofolate reductase in protozoa. Faseb J 4:1591\u20131597","journal-title":"Faseb J"},{"key":"763_CR17","doi-asserted-by":"crossref","first-page":"43126","DOI":"10.1074\/jbc.M206523200","volume":"277","author":"EF Johnson","year":"2002","unstructured":"Johnson EF, Hinz W, Atreya CE, Maley F, Anderson KS (2002) Mechanistic characterization of Toxoplasma gondii thymidylate synthase (TS-DHFR)-dihydrofolate reductase. Evidence for a TS intermediate and TS half-sites reactivity. J Biol Chem 277:43126\u201343136","journal-title":"J Biol Chem"},{"key":"763_CR18","doi-asserted-by":"crossref","first-page":"656","DOI":"10.7164\/antibiotics.36.656","volume":"36","author":"F Kanai","year":"1983","unstructured":"Kanai F, Sawa T, Hamada M, Naganawa H, Takeuchi T, Umezawa H (1983) Vanoxonin, a new inhibitor of thymidylate synthetase. J Antibiot (Tokyo) 36:656\u2013660","journal-title":"J Antibiot (Tokyo)"},{"key":"763_CR19","doi-asserted-by":"crossref","first-page":"31","DOI":"10.7164\/antibiotics.38.31","volume":"38","author":"F Kanai","year":"1985","unstructured":"Kanai F, Isshiki K, Umezawa Y, Morishima H, Naganawa H, Takita T, Takeuchi T, Umezawa H (1985a) Vanoxonin, a new inhibitor of thymidylate synthetase. II. Structure determination and total synthesis. J Antibiot (Tokyo) 38:31\u201338","journal-title":"J Antibiot (Tokyo)"},{"key":"763_CR20","doi-asserted-by":"crossref","first-page":"39","DOI":"10.7164\/antibiotics.38.39","volume":"38","author":"F Kanai","year":"1985","unstructured":"Kanai F, Kaneko T, Morishima H, Isshiki K, Takita T, Takeuchi T, Umezawa H (1985b) Vanoxonin, a new inhibitor of thymidylate synthetase. III. Inhibition of thymidylate synthetase by vanoxonin-vanadium complex. J Antibiot (Tokyo) 38:39\u201350","journal-title":"J Antibiot (Tokyo)"},{"key":"763_CR21","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1042\/BJ20050608","volume":"393","author":"A Kanai","year":"2006","unstructured":"Kanai A, Sato A, Imoto J, Tomita M (2006) Archaeal Pyrococcus furiosus thymidylate synthase 1 is an RNA-binding protein. Biochem J 393:373\u2013379","journal-title":"Biochem J"},{"key":"763_CR22","doi-asserted-by":"crossref","first-page":"2441","DOI":"10.1021\/bi00008a006","volume":"34","author":"JT Kealey","year":"1995","unstructured":"Kealey JT, Santi DV (1995) Stereochemistry of tRNA(m5U54)-methyltransferase catalysis: 19F NMR spectroscopy of an enzyme-FUraRNA covalent complex. Biochemistry 34:2441\u20132446","journal-title":"Biochemistry"},{"key":"763_CR23","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1016\/0300-9084(94)90042-6","volume":"76","author":"JT Kealey","year":"1994","unstructured":"Kealey JT, Gu X, Santi DV (1994) Enzymatic mechanism of tRNA (m5U54)methyltransferase. Biochimie 76:1133\u20131142","journal-title":"Biochimie"},{"key":"763_CR24","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1002\/prot.10202","volume":"49","author":"P Kuhn","year":"2002","unstructured":"Kuhn P, Lesley SA, Mathews II, Canaves JM, Brinen LS, Dai X, Deacon AM, Elsliger MA, Eshaghi S, Floyd R, Godzik A, Grittini C, Grzechnik SK, Guda C, Hodgson KO, Jaroszewski L, Karlak C, Klock HE, Koesema E, Kovarik JM, Kreusch AT, McMullan D, McPhillips TM, Miller MA, Miller M, Morse A, Mon K, Ouyang J, Robb A, Rodrigues K, Selby TL, Spraggon G, Stevens RC, Taylor SS, Van den Bedem H, Velasquez J, Vincent J, Wang X, West B, Wolf G, Wooley J, Wilson IA (2002) Crystal structure of thy1, a thymidylate synthase complementing protein from thermotoga maritima at 2.25\u00a0\u00c5 resolution. Protein Struc Funct Genet 49:142\u2013145","journal-title":"Protein Struc Funct Genet"},{"key":"763_CR25","doi-asserted-by":"crossref","first-page":"7252","DOI":"10.1073\/pnas.0401365101","volume":"101","author":"D Leduc","year":"2004","unstructured":"Leduc D, Graziani S, Lipowski G, Marchand C, Le Mar\u00e9chal P, Liebl U, Myllykallio H (2004a) Functional evidence for active site location of tetrameric thymidylate synthase X at the interphase of three monomers. Proc Natl Acad Sci USA 101:7252\u20137257","journal-title":"Proc Natl Acad Sci USA"},{"key":"763_CR26","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1042\/bst0320231","volume":"32","author":"D Leduc","year":"2004","unstructured":"Leduc D, Graziani S, Meslet-Cladiere L, Sodolescu A, Liebl U, Myllykallio H (2004b) Two distinct pathways for thymidylate (dTMP) synthesis in (hyper)thermophilic Bacteria and Archaea. Biochem Soc Trans 32:231\u2013235","journal-title":"Biochem Soc Trans"},{"key":"763_CR27","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.str.2004.02.009","volume":"12","author":"TT Lee","year":"2004","unstructured":"Lee TT, Agarwalla S, Stroud RM (2004) Crystal structure of RumA, an iron\u2013sulfur cluster containing E. coli ribosomal RNA 5-methyluridine methyltransferase. Structure 12:397\u2013407","journal-title":"Structure"},{"key":"763_CR28","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/j.cell.2004.12.037","volume":"120","author":"TT Lee","year":"2005","unstructured":"Lee TT, Agarwalla S, Stroud RM (2005) A unique RNA Fold in the RumA-RNA-cofactor ternary complex contributes to substrate selectivity and enzymatic function. Cell 120:599\u2013611","journal-title":"Cell"},{"key":"763_CR29","doi-asserted-by":"crossref","first-page":"11664","DOI":"10.1073\/pnas.142413399","volume":"99","author":"SA Lesley","year":"2002","unstructured":"Lesley SA, Kuhn P, Godzik A, Deacon AM, Mathews I, Kreusch A, Spraggon G, Klock HE, McMullan D, Shin T, Vincent J, Robb A, Brinen LS, Miller MD, McPhillips TM, Miller MA, Scheibe D, Canaves JM, Guda C, Jaroszewski L, Selby TL, Elsliger M-A, Wooley J, Taylor SS, Hodgson KO, Wilson IA, Schultz PG, Stevens RC (2002) Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline. Proc Natl Acad Sci USA 99:11664\u201311669","journal-title":"Proc Natl Acad Sci USA"},{"key":"763_CR30","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/S0925-4439(02)00080-7","volume":"1587","author":"J Liu","year":"2002","unstructured":"Liu J, Schmitz JC, Lin X, Tai N, Yan W, Farrell M, Bailly M, Chen T, Chu E (2002) Thymidylate synthase as a translational regulator of cellular gene expression. Biochim Biophys Acta 1587:174\u2013182","journal-title":"Biochim Biophys Acta"},{"key":"763_CR31","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1039\/b517881a","volume":"16","author":"A Mason","year":"2006","unstructured":"Mason A, Agrawal N, Washington MT, Lesley SA, Kohen A (2006) A lag-phase in the reduction of flavin dependent thymidylate synthase (FDTS) revealed a mechanistic missing link. Chem Commun 16:1781\u20131783","journal-title":"Chem Commun (Camb.)"},{"key":"763_CR32","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1016\/S0969-2126(03)00097-2","volume":"11","author":"II Mathews","year":"2003","unstructured":"Mathews II, Deacon AM, Canaves JM, McMullan D, Lesley SA, Agarwalla S, Kuhn P (2003) Functional analysis of substrate and cofactor complex structures of a thymidylate synthase-complementing protein. Structure 11:677\u2013690","journal-title":"Structure"},{"key":"763_CR33","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.tibs.2006.03.003","volume":"31","author":"A Mattevi","year":"2006","unstructured":"Mattevi A (2006) To be or not to be an oxidase: challenging the oxygen reactivity of flavoenzymes. Trends Biochem Sci 31:276\u2013283","journal-title":"Trends Biochem Sci"},{"key":"763_CR34","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1126\/science.1073910","volume":"297","author":"AG Murzin","year":"2002","unstructured":"Murzin AG (2002) Biochemistry. DNA building block reinvented. Science 297:61\u201362","journal-title":"Science"},{"key":"763_CR35","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1126\/science.1072113","volume":"297","author":"H Myllykallio","year":"2002","unstructured":"Myllykallio H, Lipowski G, Leduc D, Filee J, Forterre P, Liebl U (2002) An alternative flavin-dependent mechanism of thymidylate synthesis. Science 297:105\u2013107","journal-title":"Science"},{"key":"763_CR36","doi-asserted-by":"crossref","first-page":"3995","DOI":"10.1073\/pnas.89.9.3995","volume":"89","author":"BC Persson","year":"1992","unstructured":"Persson BC, Grustafsson C, Berg DE, Bjork GR (1992) The gene for a tRNA modifying enzyme, m5U54-methyltransferase, is essential for viability in Escherichia coli. Proc Natl Acad Sci USA 89:3995\u20133998","journal-title":"Proc Natl Acad Sci USA"},{"key":"763_CR37","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/S1096-7176(03)00008-9","volume":"5","author":"P Pharkya","year":"2003","unstructured":"Pharkya P, Nikolaev EV, Maranas CD (2003) Review of the BRENDA Database. Metab Eng 5:71\u201373","journal-title":"Metab Eng"},{"key":"763_CR38","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1016\/S0006-291X(74)80419-5","volume":"61","author":"JM Romeo","year":"1974","unstructured":"Romeo JM, Delk AS, Rabinowitz JC (1974) The occurrence of a transmethylation reaction not involving S-adenosylmethionine in the formation of ribothymidine in Bacillus subtilis transfer-RNA. Biochem Biophys Res Commun 61:1256\u20131261","journal-title":"Biochem Biophys Res Commun"},{"key":"763_CR39","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1016\/j.jmb.2005.07.071","volume":"352","author":"P Sampathkumar","year":"2005","unstructured":"Sampathkumar P, Turley S, Ulmer JE, Rhie HG, Sibley CH, Hol WGJ (2005) Structure of the Mycobacterium tuberculosis flavin dependent thymidylate synthase (MtbThyX) at 2.0 resolution. J Mol Biol 352:1091\u20131104","journal-title":"J Mol Biol"},{"key":"763_CR40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jmb.2006.04.061","volume":"360","author":"P Sampathkumar","year":"2006","unstructured":"Sampathkumar P, Turley S, Sibley CH, Hol WG (2006) NADP(+) expels both the co-factor and a substrate analog from the Mycobacterium tuberculosis ThyX active site: opportunities for anti-bacterial drug design. J Mol Biol 360:1\u20136","journal-title":"J Mol Biol"},{"key":"763_CR41","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1126\/science.8451640","volume":"259","author":"BK Shoichet","year":"1993","unstructured":"Shoichet BK, Stroud RM, Santi DV, Kuntz ID, Perry KM (1993) Structure-based discovery of inhibitors of thymidylate synthase. Science 259:1445\u20131450","journal-title":"Science"},{"key":"763_CR42","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.1021\/bi9815896","volume":"38","author":"TJ Stout","year":"1999","unstructured":"Stout TJ, Tondi D, Rinaldi M, Barlocco D, Pecorari P, Santi DV, Kuntz ID, Stroud RM, Shoichet BK, Costi MP (1999) Structure-based design of inhibitors specific for bacterial thymidylate synthase. Biochemistry 38:1607\u20131617","journal-title":"Biochemistry"},{"key":"763_CR43","doi-asserted-by":"crossref","first-page":"2521","DOI":"10.2741\/1413","volume":"9","author":"N Tai","year":"2004","unstructured":"Tai N, Schmitz JC, Liu J, Lin X, Bailly M, Chen TM, Chu E (2004) Translational autoregulation of thymidylate synthase and dihydrofolate reductase. Front Biosci 9:2521\u20132526","journal-title":"Front Biosci"},{"key":"763_CR44","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/S1074-5521(99)80077-5","volume":"6","author":"D Tondi","year":"1999","unstructured":"Tondi D, Slomczynska U, Costi MP, Watterson DM, Ghelli S, Shoichet BK (1999) Structure-based discovery and in-parallel optimization of novel competitive inhibitors of thymidylate synthase. Chem Biol 6:319\u2013331","journal-title":"Chem Biol"},{"key":"763_CR45","doi-asserted-by":"crossref","first-page":"3955","DOI":"10.1093\/nar\/gki703","volume":"33","author":"J Urbonavicius","year":"2005","unstructured":"Urbonavicius J, Skouloubris S, Myllykallio H, Grosjean H (2005) Identification of a novel gene encoding a flavin-dependent tRNA: m5U methyltransferase in bacteria-evolutionary implications. Nucleic Acids Res 33:3955\u20133964","journal-title":"Nucleic Acids Res"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-006-0763-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00253-006-0763-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-006-0763-1","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-006-0763-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,7]],"date-time":"2021-08-07T12:45:20Z","timestamp":1628340320000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00253-006-0763-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,2]]},"references-count":45,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2007,2]]}},"alternative-id":["763"],"URL":"https:\/\/doi.org\/10.1007\/s00253-006-0763-1","relation":{},"ISSN":["0175-7598","1432-0614"],"issn-type":[{"value":"0175-7598","type":"print"},{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,2]]}}}