{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T03:05:02Z","timestamp":1781665502151,"version":"3.54.5"},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2010,5,23]],"date-time":"2010-05-23T00:00:00Z","timestamp":1274572800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"published-print":{"date-parts":[[2010,8]]},"DOI":"10.1007\/s00253-010-2662-8","type":"journal-article","created":{"date-parts":[[2010,5,22]],"date-time":"2010-05-22T10:26:30Z","timestamp":1274523990000},"page":"1773-1782","source":"Crossref","is-referenced-by-count":44,"title":["Engineering of a fungal \u03b2-galactosidase to remove product inhibition by galactose"],"prefix":"10.1007","volume":"87","author":[{"given":"Xuejun","family":"Hu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvain","family":"Robin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shane","family":"O\u2019Connell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Walsh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Gerard","family":"Wall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2010,5,23]]},"reference":[{"key":"2662_CR1","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/j.jmb.2004.05.028","volume":"340","author":"JD Bendtsen","year":"2004","unstructured":"Bendtsen JD, Nielsen H, von Heijne G, Brunak S (2004) Improved prediction of signal peptides: signalP 3.0. J Mol Biol 340:783\u2013795","journal-title":"J Mol Biol"},{"key":"2662_CR2","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1093\/nar\/gkg095","volume":"31","author":"B Boeckmann","year":"2003","unstructured":"Boeckmann B, Bairoch A, Apweiler R, Blatter MC, Estreicher A, Gasteiger E, Martin MJ, Michoud K, O'Donovan C, Phan I, Pilbout S, Schneider M (2003) The SWISS-PROT protein knowledgebase and its supplement TrEMBL in 2003. Nucleic Acids Res 31:365\u2013370","journal-title":"Nucleic Acids Res"},{"key":"2662_CR3","first-page":"36","volume":"202","author":"J Gaska","year":"1990","unstructured":"Gaska J (1990) Treatment of lactose intolerance. Am Drug 202:36\u201343","journal-title":"Am Drug"},{"key":"2662_CR4","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1007\/978-1-4615-7050-9_6","volume-title":"Fundamentals of dairy chemistry","author":"V Holsinger","year":"1988","unstructured":"Holsinger V (1988) Lactose. In: Wong P (ed) Fundamentals of dairy chemistry. Van Nostrand Reinhold, NY, pp 279\u2013341"},{"key":"2662_CR5","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/0378-1119(89)90359-4","volume":"77","author":"RM Horton","year":"1989","unstructured":"Horton RM, Hunt HD, Ho SN, Pullen JK, Pease LR (1989) Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77:61\u201368","journal-title":"Gene"},{"key":"2662_CR6","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jbiotec.2005.05.018","volume":"120","author":"X Hu","year":"2005","unstructured":"Hu X, O\u2019Dwyer R, Wall JG (2005) Cloning, expression and characterisation of a single-chain Fv antibody fragment against domoic acid in Escherichia coli. J Biotechnol 120:38\u201345","journal-title":"J Biotechnol"},{"key":"2662_CR7","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.pep.2006.08.009","volume":"52","author":"X Hu","year":"2007","unstructured":"Hu X, O\u2019Hara L, White S, Magner E, Kane M, Wall JG (2007) Optimisation of production of a domoic acid-binding scFv antibody fragment in Escherichia coli using molecular chaperones and functional immobilisation on a mesoporous silicate support. Protein Expr Purif 52:194\u2013201","journal-title":"Protein Expr Purif"},{"key":"2662_CR8","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/S0141-0229(02)00107-2","volume":"31","author":"E Jurado","year":"2002","unstructured":"Jurado E, Camacho F, Luzon G, Vicaria JM (2002) A new kinetic model proposed for enzymatic hydrolysis of lactose by a \u03b2-galactosidase from Kluyveromyces fragilis. Enzyme Microb Technol 31:300\u2013309","journal-title":"Enzyme Microb Technol"},{"key":"2662_CR9","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1093\/protein\/gzj015","volume":"19","author":"Y Kai","year":"2006","unstructured":"Kai Y, Kashiwagi T, Ishikawa K, Ziyatdinov MK, Redkina EI, Kiriukhin MY, Gusyatiner MM, Kobayashi S, Takagi H, Suzuki E (2006) Engineering of Escherichia coli L-serine O-acetyltransferase on the basis of crystal structure: desensitization to feedback inhibition by L-cysteine. Protein Eng Des Sel 19:163\u2013167","journal-title":"Protein Eng Des Sel"},{"key":"2662_CR10","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1016\/S1046-5928(02)00036-0","volume":"25","author":"N Koganesawa","year":"2002","unstructured":"Koganesawa N, Aizava T, Shimojo H, Miura K, Ohnishi A, Demura M, Hayakawa Y, Nitta K, Kawano K (2002) Expression and purification of a small cytokine growth-blocking peptide from armyworm Pseudaletia separata by an optimized fermentation method using the methylotrophic yeast Pichia pastoris. Protein Expr Purif 25:416\u2013425","journal-title":"Protein Expr Purif"},{"key":"2662_CR11","doi-asserted-by":"crossref","first-page":"91","DOI":"10.2174\/138920310790274617","volume":"11","author":"NE Labrou","year":"2010","unstructured":"Labrou NE (2010) Random mutagenesis methods for in vitro directed enzyme evolution. Curr Protein Pept Sci 11:91\u2013100","journal-title":"Curr Protein Pept Sci"},{"key":"2662_CR12","doi-asserted-by":"crossref","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","volume":"23","author":"MA Larkin","year":"2007","unstructured":"Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) ClustalW2 and ClustalX version 2. Bioinformatics 23:2947\u20132948","journal-title":"Bioinformatics"},{"key":"2662_CR13","doi-asserted-by":"crossref","first-page":"2022","DOI":"10.1007\/BF01297079","volume":"38","author":"MY Lin","year":"1993","unstructured":"Lin MY, Dipalma JA, Martini MC, Gross CJ, Harlander SK, Savaiano DA (1993) Comparative effects of exogenous lactase (\u03b2-galactosidase) preparations on in vivo lactose digestion. Dig Dis Sci 38:2022\u20132027","journal-title":"Dig Dis Sci"},{"key":"2662_CR14","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1002\/yea.1208","volume":"22","author":"S Macauley-Patrick","year":"2005","unstructured":"Macauley-Patrick S, Fazenda ML, McNeil B, Harvey LM (2005) Heterologous protein production using the Pichia pastoris expression system. Yeast 22:249\u2013270","journal-title":"Yeast"},{"key":"2662_CR15","first-page":"1291","volume-title":"Encyclopedia of bioprocess technology, fermentation, biocatalysis and bioseparation","author":"T Nakayama","year":"1999","unstructured":"Nakayama T, Amachi T (1999) \u03b2-Galactosidase. In: Flinckinger MC, Drew SW (eds) Encyclopedia of bioprocess technology, fermentation, biocatalysis and bioseparation, 1st edn. Wiley, NY, pp 1291\u20131305","edition":"1"},{"key":"2662_CR16","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1385\/ABAB:134:2:179","volume":"134","author":"S O\u2019Connell","year":"2006","unstructured":"O\u2019Connell S, Walsh G (2006) Physicochemical characteristics of commercial lactases relevant to their application in the alleviation of lactose intolerance. Appl Biochem Biotechnol 134:179\u2013191","journal-title":"Appl Biochem Biotechnol"},{"key":"2662_CR17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s12010-007-9206-4","volume":"141","author":"S O\u2019Connell","year":"2007","unstructured":"O\u2019Connell S, Walsh G (2007) Purification and properties of a \u03b2-galactosidase with potential application as a digestive supplement. Appl Biochem Biotechnol 141:1\u201314","journal-title":"Appl Biochem Biotechnol"},{"key":"2662_CR18","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1007\/s00253-009-2270-7","volume":"86","author":"S O\u2019Connell","year":"2010","unstructured":"O\u2019Connell S, Walsh G (2010) A novel acid-stable, acid-active \u03b2-galactosidase potentially suited to the alleviation of lactose intolerance. Appl Microbiol Biotechnol 86:517\u2013524","journal-title":"Appl Microbiol Biotechnol"},{"key":"2662_CR19","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1271\/bbb.65.1149","volume":"65","author":"Y Ogawa-Miyata","year":"2001","unstructured":"Ogawa-Miyata Y, Kojima H, Sano K (2001) Mutation analysis of the feedback inhibition site of aspartokinase III of Escherichia coli K-12 and its use in L-threonine production. Biosci Biotechnol Biochem 65:1149\u20131154","journal-title":"Biosci Biotechnol Biochem"},{"key":"2662_CR20","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.1093\/bioinformatics\/bti203","volume":"21","author":"G Pollastri","year":"2005","unstructured":"Pollastri G, McLysaght A (2005) Porter: a new, accurate server for protein secondary structure prediction. Bioinformatics 21:1719\u20131720","journal-title":"Bioinformatics"},{"key":"2662_CR21","first-page":"566","volume":"89","author":"F Ramirez","year":"1994","unstructured":"Ramirez F, Lee K, Graham D (1994) All lactase preparations are not the same: results of a prospective, randomized, placebo-controlled trial. Am J Gastroenterol 89:566\u2013570","journal-title":"Am J Gastroenterol"},{"key":"2662_CR22","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1111\/j.1365-2672.1994.tb03435.x","volume":"77","author":"I Rasouli","year":"1994","unstructured":"Rasouli I, Kulkarni PR (1994) Enhancement of \u03b2-galactosidase productivity of Aspergillus niger NCIM-616. J Appl Bacteriol 77:359\u2013361","journal-title":"J Appl Bacteriol"},{"key":"2662_CR23","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1016\/j.jmb.2004.09.012","volume":"343","author":"AL Rojas","year":"2004","unstructured":"Rojas AL, Nagem RA, Neustroev KN, Arand M, Adamska M, Eneyskaya EV, Kulminskaya AA, Garratt RC, Golubev AM, Polikarpov I (2004) Crystal structures of \u03b2-galactosidase from Penicillium sp. and its complex with galactose. J Mol Biol 343:1281\u20131292","journal-title":"J Mol Biol"},{"key":"2662_CR24","volume-title":"Molecular cloning. A laboratory manual","author":"J Sambrook","year":"2001","unstructured":"Sambrook J, Russell DW (2001) Molecular cloning. A laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, New York","edition":"3"},{"key":"2662_CR25","first-page":"533","volume":"11","author":"S Sanders","year":"1992","unstructured":"Sanders S, Tolman K, Reitberg D (1992) Effect of a single dose of lactase on symptoms and expired hydrogen after lactose challenge in lactose-intolerant subjects. Clin Pharm 11:533\u2013538","journal-title":"Clin Pharm"},{"key":"2662_CR26","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/S0014-5793(99)00679-1","volume":"452","author":"H Takagi","year":"1999","unstructured":"Takagi H, Kobayashi C, Kobayashi S, Nakamori S (1999) PCR random mutagenesis into Escherichia coli serine acetyltransferase: isolation of the mutant enzymes that cause overproduction of L-cysteine and L-cystine due to the desensitization to feedback inhibition. FEBS Lett 452:323\u2013327","journal-title":"FEBS Lett"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-010-2662-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00253-010-2662-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-010-2662-8","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,24]],"date-time":"2019-05-24T15:17:36Z","timestamp":1558711056000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00253-010-2662-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,5,23]]},"references-count":26,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2010,8]]}},"alternative-id":["2662"],"URL":"https:\/\/doi.org\/10.1007\/s00253-010-2662-8","relation":{},"ISSN":["0175-7598","1432-0614"],"issn-type":[{"value":"0175-7598","type":"print"},{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,5,23]]}}}