{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T21:19:04Z","timestamp":1768684744382,"version":"3.49.0"},"reference-count":52,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2012,2,16]],"date-time":"2012-02-16T00:00:00Z","timestamp":1329350400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"published-print":{"date-parts":[[2012,3]]},"DOI":"10.1007\/s00253-012-3930-6","type":"journal-article","created":{"date-parts":[[2012,2,15]],"date-time":"2012-02-15T04:19:21Z","timestamp":1329279561000},"page":"2291-2300","source":"Crossref","is-referenced-by-count":32,"title":["Strategies to overexpress enterotoxigenic Escherichia coli (ETEC) colonization factors for the construction of oral whole-cell inactivated ETEC vaccine candidates"],"prefix":"10.1007","volume":"93","author":[{"given":"Joshua","family":"Tobias","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann-Mari","family":"Svennerholm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2012,2,16]]},"reference":[{"key":"3930_CR1","doi-asserted-by":"crossref","first-page":"7190","DOI":"10.1128\/IAI.72.12.7190-7201.2004","volume":"72","author":"RP Anantha","year":"2004","unstructured":"Anantha RP, McVeigh AL, Lee LH, Agnew MK, Cassels FJ, Scott DA, Whittam S, Savarino SJ (2004) Evolutionary and functional relationships of colonization factor antigen i and other class 5 adhesive fimbriae of enterotoxigenic Escherichia coli. Infect Immun 72:7190\u20137201","journal-title":"Infect Immun"},{"key":"3930_CR2","doi-asserted-by":"crossref","first-page":"2127","DOI":"10.1128\/IAI.65.6.2127-2135.1997","volume":"65","author":"JR Butterton","year":"1997","unstructured":"Butterton JR, Ryan ET, Acheson DWK, Calderwood SB (1997) Coexpression of the B subunit of Shiga toxin 1 and EaeA from enterohemorrhagic Escherichia coli in Vibrio cholerae vaccine strains. Infect Immun 65:2127\u20132135","journal-title":"Infect Immun"},{"key":"3930_CR3","doi-asserted-by":"crossref","first-page":"874","DOI":"10.1128\/IAI.58.4.874-878.1990","volume":"58","author":"J Caron","year":"1990","unstructured":"Caron J, Scott JR (1990) A rns-like regulatory gene for colonization factor antigen I (CFA\/I) that controls expression of CFA\/I pilin. Infect Immun 58:874\u2013878","journal-title":"Infect Immun"},{"key":"3930_CR4","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1073\/pnas.86.3.963","volume":"86","author":"J Caron","year":"1989","unstructured":"Caron J, Coffield LM, Scott JR (1989) A plasmid-encoded regulatory gene, rns, required for expression of the CS1 and CS2 adhesins of enterotoxigenic Escherichia coli. Proc Natl Acad Sci U S A 86:963\u2013967","journal-title":"Proc Natl Acad Sci U S A"},{"key":"3930_CR5","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1007\/BF01569828","volume":"15","author":"FJ Cassels","year":"1995","unstructured":"Cassels FJ, Wolf MK (1995) Colonization factors of diarrheagenic E. coli and their intestinal receptors. J Ind Microbiol 15:214\u2013226","journal-title":"J Ind Microbiol"},{"key":"3930_CR6","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/0378-1097(91)90499-Z","volume":"67","author":"LA Haan de","year":"1991","unstructured":"de Haan LA, Willshaw GA, van der Zeijst BA, Gaastra W (1991) The nucleotide sequence of a regulatory gene present on a plasmid in an enterotoxigenic Escherichia coli strain of serotype O167:H5. FEMS Microbiol Lett 67:341\u2013346","journal-title":"FEMS Microbiol Lett"},{"key":"3930_CR7","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1128\/jb.184.4.1065-1077.2002","volume":"184","author":"TG Duthy","year":"2002","unstructured":"Duthy TG, Staendner LH, Manning PA, Heuzenbroeder MW (2002) Identification and characterization of assembly proteins of CS5 pili from enterotoxigenic Escherichia coli. J Bacteriol 184:1065\u20131077","journal-title":"J Bacteriol"},{"key":"3930_CR8","doi-asserted-by":"crossref","first-page":"4354","DOI":"10.1016\/j.vaccine.2006.02.052","volume":"24","author":"D Favre","year":"2006","unstructured":"Favre D, L\u00fcdi S, Stoffel M, Frey J, Horn MP, Dietrich G, Spreng S, Viret JF (2006) Expression of enterotoxigenic Escherichia coli colonization factors in Vibrio cholerae. Vaccine 24:4354\u20134368","journal-title":"Vaccine"},{"key":"3930_CR9","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1006\/mpat.2000.0436","volume":"30","author":"A Frisk","year":"2001","unstructured":"Frisk A, Lebens M, Johansson C, Ahmed H, Svensson L, Ahlman K, Lagerg\u00e5rd T (2001) The role of different protein components from the Haemophilus ducreyi cytolethal distending toxin in the generation of cell toxicity. Microb Pathog 30:313\u2013324","journal-title":"Microb Pathog"},{"key":"3930_CR10","doi-asserted-by":"crossref","first-page":"48549","DOI":"10.1128\/IAI.63.12.4849-4856.1995","volume":"63","author":"BJ Froehlich","year":"1995","unstructured":"Froehlich BJ, Karakashian A, Sakellaris H, Scott JR (1995) Genes for CS2 pili of enterotoxigenic Escherichia coli and their interchangeability with those for CS1 pili. Infect Immun 63:48549\u201348556","journal-title":"Infect Immun"},{"key":"3930_CR11","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/0966-842X(96)10068-8","volume":"4","author":"W Gaastra","year":"1996","unstructured":"Gaastra W, Svennerholm AM (1996) Colonization factors of human enterotoxigenic Escherichia coli (ETEC). Trends Microbiol 4:444\u2013452","journal-title":"Trends Microbiol"},{"key":"3930_CR12","doi-asserted-by":"crossref","first-page":"2853","DOI":"10.1128\/IAI.69.5.2853-2857.2001","volume":"69","author":"ER Hall","year":"2001","unstructured":"Hall ER, Wierzba TF, \u00c5hr\u00e9n C, Rao MR, Bassily S, Francis W, Girgis FY, Safwat M, Lee YJ, Svennerholm A-M, Clemens JD, Savarino SJ (2001) Induction of systemic antifimbria and antitoxin antibody responses in Egyptian children and adults by an oral, killed enterotoxigenic Escherichia coli plus cholera toxin B subunit vaccine. Infect Immun 69:2853\u20132857","journal-title":"Infect Immun"},{"key":"3930_CR13","doi-asserted-by":"crossref","first-page":"6167","DOI":"10.1016\/j.vaccine.2011.06.084","volume":"29","author":"SD Isidean","year":"2011","unstructured":"Isidean SD, Riddle MS, Savarino SJ, Porter CK (2011) A systematic review of ETEC epidemiology focusing on colonization factor and toxin expression. Vaccine 29:6167\u20136178","journal-title":"Vaccine"},{"key":"3930_CR14","doi-asserted-by":"crossref","first-page":"3488","DOI":"10.1128\/IAI.02006-05","volume":"74","author":"L Jansson","year":"2006","unstructured":"Jansson L, Tobias J, Lebens M, Svennerholm AM, Teneberg S (2006) Glycosphingolipid binding specificity of colonization factor antigen I from enterotoxigenic Escherichia coli. Infect Immun 74:3488\u20133497","journal-title":"Infect Immun"},{"key":"3930_CR15","doi-asserted-by":"crossref","first-page":"e.4487","DOI":"10.1371\/journal.pone.0004487","volume":"4","author":"L Jansson","year":"2009","unstructured":"Jansson L, Tobias J, Jarefj\u00e4ll C, Lebens M, Svennerholm AM, Teneberg S (2009) Sulfatide recognition by colonization factor antigen CS6 from enterotoxigenic Escherichia coli. PlosOne 4:e.4487","journal-title":"PlosOne"},{"key":"3930_CR16","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1016\/0300-9084(94)90029-9","volume":"76","author":"BJ Jordi","year":"1994","unstructured":"Jordi BJ, van der Zeijst BA, Gaastra W (1994) Regions of the CFA\/I promoter involved in the activation by the transcriptional activator CfaD and repression by the histone-like protein H-NS. Biochimie 76:1052\u20131054","journal-title":"Biochimie"},{"key":"3930_CR17","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1128\/IAI.23.3.729-736.1979","volume":"23","author":"MM Levine","year":"1979","unstructured":"Levine MM, Nalin DR, Hoover DL, Bergquist EJ, Hornick BB, Young CR (1979) Immunity to enterotoxigenic Escherichia coli. Infect Immun 23:729\u2013736","journal-title":"Infect Immun"},{"key":"3930_CR18","doi-asserted-by":"crossref","unstructured":"Li YF, Poole S, Rasulova F, McVeigh AL, Savarino SJ, Xia D (2007) A receptor-binding site as revealed by the crystal structure of CfaE, the colonization factor antigen I fimbrial adhesin of enterotoxigenic Escherichia coli. J Biol Chem 282:23970\u201323980","DOI":"10.1074\/jbc.M700921200"},{"key":"3930_CR19","doi-asserted-by":"crossref","first-page":"10793","DOI":"10.1073\/pnas.0812843106","volume":"106","author":"YF Li","year":"2009","unstructured":"Li YF, Poole S, Nishio K, Jang K, Rasulova F, McVeigh A, Savarino SJ, Xia D, Bullitt E (2009) Structure of CFA\/I fimbriae from enterotoxigenic Escherichia coli. Proc Natl Acad Sci U S A 106:10793\u201310798","journal-title":"Proc Natl Acad Sci U S A"},{"key":"3930_CR20","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.1128\/JCM.26.10.1967-1972.1988","volume":"26","author":"Y L\u00f3pez-Vidal","year":"1988","unstructured":"L\u00f3pez-Vidal Y, Klemm P, Svennerholm AM (1988) Monoclonal antibodies against different epitopes on colonization factor antigen I of enterotoxin-producing Escherichia coli. J Clin Microbiol 26:1967\u20131972","journal-title":"J Clin Microbiol"},{"key":"3930_CR21","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1093\/infdis\/162.2.442","volume":"162","author":"Y L\u00f3pez-Vidal","year":"1990","unstructured":"L\u00f3pez-Vidal Y, Calva JJ, Trujillo A, Ponce de Le\u00f3n A, Ramos A, Svennerholm AM, Ruiz-Palacios GM (1990) Enterotoxins and adhesins of enterotoxigenic Escherichia coli: are they risk factors for acute diarrhea in the community? J Infect Dis 162:442\u2013447","journal-title":"J Infect Dis"},{"key":"3930_CR22","unstructured":"Lundgren A, Leach S, Jertborn M, Kaim J, Wiklund G, Adamsson J, Eklund L, Bourgeois L, Walker R, Tobias J, Holmgren J, Svennerholm AM (2011) Clinical trial of an inactivated whole cell ETEC prototype vaccine. The 6th international conference on vaccines for enteric diseases. Cannes, France"},{"key":"3930_CR23","doi-asserted-by":"crossref","first-page":"1974","DOI":"10.1128\/IAI.56.8.1974-1980.1988","volume":"56","author":"MM McConnell","year":"1988","unstructured":"McConnell MM, Thomas LV, Willshaw GA, Smith HR, Rowe B (1988) Genetic control and properties of coli surface antigens of colonization factor antigen IV (PCF8775) of enterotoxigenic Escherichia coli. Infect Immun 56:1974\u20131980","journal-title":"Infect Immun"},{"key":"3930_CR24","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1093\/infdis\/153.6.1108","volume":"153","author":"RL Owen","year":"1986","unstructured":"Owen RL, Pierce NF, Apple RT, Cray WC Jr (1986) M cell transport of Vibrio cholerae from the intestinal lumen into Peyer's patches: a mechanism for antigen sampling and for microbial transepithelial migration. J Infect Dis 153:1108\u20131118","journal-title":"J Infect Dis"},{"key":"3930_CR25","doi-asserted-by":"crossref","first-page":"3594","DOI":"10.1128\/IAI.58.11.3594-3600.1990","volume":"58","author":"J Perez-Casal","year":"1990","unstructured":"Perez-Casal J, Swartley JS, Scott JR (1990) Gene encoding the major subunit of CS1 pili of human enterotoxigenic Escherichia coli. Infect Immun 58:3594\u20133600","journal-title":"Infect Immun"},{"key":"3930_CR26","doi-asserted-by":"crossref","first-page":"1372","DOI":"10.1111\/j.1365-2958.2007.05612.x","volume":"63","author":"ST Poole","year":"2007","unstructured":"Poole ST, McVeigh AL, Anantha RP, Lee LH, Akay YM, Pontzer EA, Scott DA, Bullitt E, Savarino SJ (2007) Donor strand complementation governs intersubunit interaction of fimbriae of the alternate chaperone pathway. Mol Microbiol 63:1372\u20131384","journal-title":"Mol Microbiol"},{"key":"3930_CR27","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1128\/CMR.18.3.465-483.2005","volume":"18","author":"F Qadri","year":"2005","unstructured":"Qadri F, Svennerholm AM, Faruque ASG, Sack RB (2005) Enterotoxigenic Escherichia coli (ETEC): epidemiology, microbiology, and clinical features in developing countries. Clin Microbiol Rev 18:465\u2013483","journal-title":"Clin Microbiol Rev"},{"key":"3930_CR28","doi-asserted-by":"crossref","first-page":"1726","DOI":"10.1016\/j.vaccine.2005.08.110","volume":"24","author":"F Qadri","year":"2006","unstructured":"Qadri F, Ahmed T, Ahmed F, Begum YA, Sack DA, Svennerholm AM (2006) Reduced doses of oral killed enterotoxigenic Escherichia coli plus cholera toxin B subunit vaccine is safe and immunogenic in Bangladeshi infants 6\u201317\u00a0months of age: dosing studies in different age groups. Vaccine 24:1726\u20131733","journal-title":"Vaccine"},{"key":"3930_CR29","doi-asserted-by":"crossref","first-page":"3961","DOI":"10.1128\/IAI.00459-07","volume":"75","author":"F Qadri","year":"2007","unstructured":"Qadri F, Saha A, Ahmed T, Al Tarique A, Begum YA, Svennerholm AM (2007) Disease burden due to enterotoxigenic Escherichia coli in the first 2\u00a0years of life in an urban community in Bangladesh. Infect Immun 75:3961\u20133968","journal-title":"Infect Immun"},{"key":"3930_CR30","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1128\/IAI.73.1.258-267.2005","volume":"73","author":"RT Ranallo","year":"2005","unstructured":"Ranallo RT, Fonseka CP, Cassels F, Srinivasan J, Venkatesan MM (2005) Construction and characterization of bivalent Shigella flexneri 2a vaccine strains SC608(pCFAI) and SC608(pCFAI\/LTB) that express antigens from enterotoxigenic Escherichia coli. Infect Immun 73:258\u2013267","journal-title":"Infect Immun"},{"key":"3930_CR31","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1006\/mpat.1996.0040","volume":"21","author":"A Rudin","year":"1996","unstructured":"Rudin A, Olbe L, Svennerholm AM (1996) Monoclonal antibodies against fimbrial subunits of colonization factor antigen I (CFA\/I) inhibit binding to human enterocytes and protect against enterotoxigenic Escherichia coli expressing heterologous colonization factors. Microb Pathog 21:35\u201345","journal-title":"Microb Pathog"},{"key":"3930_CR32","doi-asserted-by":"crossref","first-page":"4392","DOI":"10.1016\/j.vaccine.2007.03.034","volume":"25","author":"DA Sack","year":"2007","unstructured":"Sack DA, Shimko J, Torres O, Bourgeois AL, Francia DS, Gustafsson B, K\u00e4rnell A, Nyquist I, Svennerholm AM (2007) Randomised, double-blind, safety and efficacy of a killed oral vaccine for enterotoxigenic Escherichia coli diarrhoea of travellers to Guatemala and Mexico. Vaccine 25:4392\u20134400","journal-title":"Vaccine"},{"key":"3930_CR33","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1046\/j.1365-2567.2002.01413.x","volume":"106","author":"H Sadeghi","year":"2002","unstructured":"Sadeghi H, Bregenholt S, Wegmann D, Petersen JS, Holmgren J, Lebens M (2002) Genetic fusion of human insulin B-chain to the B-subunit of cholera toxin enhances in vitro antigen presentation and induction of bystander suppression in vivo. Immunology 106:237\u2013245","journal-title":"Immunology"},{"key":"3930_CR34","doi-asserted-by":"crossref","first-page":"1694","DOI":"10.1128\/JB.181.5.1694-1697.1999","volume":"181","author":"H Sakellaris","year":"1999","unstructured":"Sakellaris H, Penumalli VR, Scott JR (1999) The level of expression of the minor pilin subunit, CooD, determines the number of CS1 pili assembled on the cell surface of Escherichia coli. J Bacteriol 181:1694\u20131697","journal-title":"J Bacteriol"},{"key":"3930_CR35","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/j.coi.2005.06.007","volume":"17","author":"J S\u00e1nchez","year":"2005","unstructured":"S\u00e1nchez J, Holmgren J (2005) Virulence factors, pathogenesis and vaccine protection in cholera and ETEC diarrhea. Curr Opin Immunol 17:388\u2013398","journal-title":"Curr Opin Immunol"},{"key":"3930_CR36","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1097\/00006454-200204000-00012","volume":"21","author":"SJ Savarino","year":"2002","unstructured":"Savarino SJ, Hall ER, Bassily S, Wierzba TF, Youssef FG, Peruski LF Jr, Abu-Elyazeed R, Rao M, Francis WM, El Mohamady H, Safwat M, Naficy AB, Svennerholm AM, Jertborn M, Lee YJ, Clemens JD, Pride Study Group (2002) Introductory evaluation of an oral, killed whole cell enterotoxigenic Escherichia coli plus cholera toxin B subunit vaccine in Egyptian infants. Pediatr Infect Dis J 21:322\u2013330","journal-title":"Pediatr Infect Dis J"},{"key":"3930_CR37","first-page":"188","volume":"133","author":"AM Svennerholm","year":"2011","unstructured":"Svennerholm AM (2011) From cholera to enterotoxigenic Escherichia coli (ETEC) vaccine development. Indian J Med Res 133:188\u2013196","journal-title":"Indian J Med Res"},{"key":"3930_CR38","first-page":"714","volume-title":"New generation vaccines","author":"A-M Svennerholm","year":"2010","unstructured":"Svennerholm A-M, Glenn G (2010) Vaccines against enterotoxigenic Escherichia coli. (ETEC). In: Levine MM et al (eds) New generation vaccines, 4th edn. Informa Healthcare, USA, pp 714\u2013722","edition":"4"},{"key":"3930_CR39","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1586\/14760584.7.6.795","volume":"7","author":"AM Svennerholm","year":"2008","unstructured":"Svennerholm AM, Tobias J (2008) Vaccines against enterotoxigenic Escherichia coli. Expert Rev Vaccines 7:795\u2013804","journal-title":"Expert Rev Vaccines"},{"key":"3930_CR40","doi-asserted-by":"crossref","first-page":"5373","DOI":"10.1016\/j.vaccine.2008.07.091","volume":"26","author":"J Tobias","year":"2008","unstructured":"Tobias J, Lebens M, K\u00e4llg\u00e5rd S, Nicklasso M, Svennerholm AM (2008a) Role of different genes in the CS6 operon for surface expression of enterotoxigenic Escherichia coli colonization factor CS6. Vaccine 26:5373\u20135380","journal-title":"Vaccine"},{"key":"3930_CR41","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1016\/j.vaccine.2007.12.009","volume":"26","author":"J Tobias","year":"2008","unstructured":"Tobias J, Lebens M, B\u00f6lin I, Wiklund G, Svennerholm AM (2008b) Construction of non-toxic Escherichia coli and Vibrio cholerae strains expressing high and immunogenic levels of enterotoxigenic E. coli colonization factor I fimbriae. Vaccine 26:743\u2013752","journal-title":"Vaccine"},{"key":"3930_CR42","doi-asserted-by":"crossref","first-page":"6977","DOI":"10.1016\/j.vaccine.2010.08.047","volume":"28","author":"J Tobias","year":"2010","unstructured":"Tobias J, Holmgren J, Hellman M, Nygren E, Lebens M, Svennerholm AM (2010a) Over-expression of major colonization factors of enterotoxigenic Escherichia coli, alone or together, on non-toxigenic E. coli bacteria. Vaccine 28:6977\u20136984","journal-title":"Vaccine"},{"key":"3930_CR43","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1007\/s00253-010-2577-4","volume":"87","author":"J Tobias","year":"2010","unstructured":"Tobias J, Holmgren J, Svennerholm AM, Lebens M (2010b) Construction and expression of immunogenic hybrid enterotoxigenic Escherichia coli CFA\/I and CS2 colonization fimbriae for use in vaccines. Appl Microbiol Biotechnol 87:1355\u20131365","journal-title":"Appl Microbiol Biotechnol"},{"key":"3930_CR44","doi-asserted-by":"crossref","first-page":"8863","DOI":"10.1016\/j.vaccine.2011.09.096","volume":"29","author":"J Tobias","year":"2011","unstructured":"Tobias J, Svennerholm AM, Carlin NIA, Lebens M, Holmgren J (2011) Construction of a non-toxigenic Escherichia coli oral vaccine strain expressing large amounts of CS6 and inducing strong intestinal and serum anti-CS6 antibody responses in mice. Vaccine 29:8863\u20138869","journal-title":"Vaccine"},{"key":"3930_CR45","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1651-2227.1999.tb14319.x","volume":"88","author":"T Vesikari","year":"1999","unstructured":"Vesikari T (1999) Rotavirus vaccine studies in Europe. Acta Paediat Suppl 88:9\u201313","journal-title":"Acta Paediat Suppl"},{"key":"3930_CR46","doi-asserted-by":"crossref","first-page":"5190","DOI":"10.1128\/IAI.61.12.5190-5197.1993","volume":"61","author":"GI Viboud","year":"1993","unstructured":"Viboud GI, Binsztein N, Svennerholm AM (1993) A new fimbrial putative colonization factor, PCFO20, in human enterotoxigenic Escherichia coli. Infect Immun 61:5190\u20135197","journal-title":"Infect Immun"},{"key":"3930_CR47","doi-asserted-by":"crossref","first-page":"2545","DOI":"10.1016\/j.vaccine.2006.12.028","volume":"25","author":"RI Walker","year":"2007","unstructured":"Walker RI, Steel D, Aguado T (2007) Analysis of strategies to successfully vaccinate infants in developing countries against enterotoxigenic E. coli (ETEC) disease. Vaccine 25:2545\u20132566","journal-title":"Vaccine"},{"key":"3930_CR48","first-page":"370","volume":"22","author":"C Wenner\u00e5s","year":"2004","unstructured":"Wenner\u00e5s C, Erling V (2004) Prevalence of enterotoxigenic Escherichia coli-associated diarrhoea and carrier state in the developing world. J Health Popul Nutr 22:370\u2013382","journal-title":"J Health Popul Nutr"},{"key":"3930_CR49","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1128\/CMR.10.4.569","volume":"10","author":"MK Wolf","year":"1997","unstructured":"Wolf MK (1997) Occurrence, distribution, and associations of O and H serogroups, colonization factor antigens, and toxins of enterotoxigenic Escherichia coli. Clin Microbiol Rev 10:569\u2013584","journal-title":"Clin Microbiol Rev"},{"key":"3930_CR50","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1111\/j.1574-6968.1997.tb10263.x","volume":"148","author":"MK Wolf","year":"1997","unstructured":"Wolf MK, de Haan LA, Cassels FJ, Willshaw GA, Warren R, Boedeker EC, Gasstra W (1997) The CS6 colonization factor of human enterotoxigenic Escherichia coli contains two heterologous major subunits. FEMS Microbiol Lett 148:35\u201342","journal-title":"FEMS Microbiol Lett"},{"key":"3930_CR51","first-page":"124","volume":"98","author":"B Yakhchali","year":"1997","unstructured":"Yakhchali B, Maning PA (1997) Epitope analysis of the CS3 fimbrial subunit of human enterotoxigenic Escherichia coli and the construction of novel CS3::ST and CS3::LT-B immunogens. Behring Inst Mitt 98:124\u2013134","journal-title":"Behring Inst Mitt"},{"key":"3930_CR52","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1111\/j.1469-0691.2007.01908.x","volume":"14","author":"V Ziethlow","year":"2008","unstructured":"Ziethlow V, Favre D, Viret JF, Frey J, Stoffel MH (2008) Assessment by electron-microscopy of recombinant Vibrio cholerae and Salmonella vaccine strains expressing enterotoxigenic Escherichia coli-specific surface antigens. Clin Microbiol Infect 14:282\u2013286","journal-title":"Clin Microbiol Infect"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-012-3930-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00253-012-3930-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-012-3930-6","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,2]],"date-time":"2020-07-02T12:29:24Z","timestamp":1593692964000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00253-012-3930-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2,16]]},"references-count":52,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2012,3]]}},"alternative-id":["3930"],"URL":"https:\/\/doi.org\/10.1007\/s00253-012-3930-6","relation":{},"ISSN":["0175-7598","1432-0614"],"issn-type":[{"value":"0175-7598","type":"print"},{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,2,16]]}}}