{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:30:43Z","timestamp":1790775043494,"version":"4.1.0"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2012,5,18]],"date-time":"2012-05-18T00:00:00Z","timestamp":1337299200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"published-print":{"date-parts":[[2013,3]]},"DOI":"10.1007\/s00253-012-4071-7","type":"journal-article","created":{"date-parts":[[2012,5,17]],"date-time":"2012-05-17T15:58:23Z","timestamp":1337270303000},"page":"1987-1995","source":"Crossref","is-referenced-by-count":83,"title":["Heterologous expression of the C-terminal antigenic domain of the malaria vaccine candidate Pfs48\/45 in the green algae Chlamydomonas reinhardtii"],"prefix":"10.1007","volume":"97","author":[{"given":"Carla S.","family":"Jones","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiffany","family":"Luong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Hannon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miller","family":"Tran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James A.","family":"Gregory","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhouxin","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven P.","family":"Briggs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen P.","family":"Mayfield","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2012,5,18]]},"reference":[{"key":"4071_CR1","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1007\/s00438-005-0055-y","volume":"274","author":"D Barnes","year":"2005","unstructured":"Barnes D, Franklin S, Schultz J, Henry R, Brown E, Coragliotti A, Mayfield SP (2005) Contribution of the 5\u2019 and 3\u2019-untranslated regions of the plastid mRNAs to expression of Chlamydomonas reinhardtii chloroplast genes. Mol Gen Genom 274:625\u2013636. doi: 10.1007\/s00438-005-0055-y","journal-title":"Mol. Gen. Genom"},{"key":"4071_CR2","doi-asserted-by":"crossref","unstructured":"Berrang-Ford I, MacLean JD, Gyorkos TW, Ford JD, Ogden NH (2009) Climate change and malaria in Canada: a systems approach. Interdiscip Perspect Infect Dis 1\u201313. doi: 10.1155\/2009\/385487","DOI":"10.1155\/2009\/385487"},{"key":"4071_CR3","doi-asserted-by":"crossref","first-page":"e14114","DOI":"10.1371\/journal.pone.0014114","volume":"5","author":"T Bousema","year":"2010","unstructured":"Bousema T, Roeffen W, Meijerink H, Mwerinde H, Mwakalinga S, van Gemert GJ, van de Vegte-Bolmer M, Mosha F, Targett G, Riley EM, Sauerwein R, Drakeley C (2010) The dynamics of naturally acquired immune responses to Plasmodium falciparum sexual stage antigens Pfs230 & Pfs48\/45 in a low endemic area in Tanzania. PLoS One 5:e14114. doi: 10.1371\/journal.pone.0014114","journal-title":"PLoS One"},{"key":"4071_CR4","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1111\/j.1365-3024.1990.tb00990.x","volume":"12","author":"R Carter","year":"1990","unstructured":"Carter R, Graves PM, Keister DB, Quakyi IA (1990) Properties of epitopes of Pfs 48\/45, a target of transmission blocking monoclonal antibodies, on gametes of different isolates of Plasmodium falciparum. Parasite Immunol 12:587\u2013603. doi: 10.1111\/j.1365-3024.1990.tb00990.x","journal-title":"Parasite Immunol"},{"key":"4071_CR5","doi-asserted-by":"crossref","first-page":"e6352","DOI":"10.1371\/journal.pone.0006352","volume":"4","author":"DR Chowdhury","year":"2009","unstructured":"Chowdhury DR, Angov E, Kariuki T, Kumar N (2009) A potent malaria transmission blocking vaccine based on codon harmonized full length Pfs48\/45 expressed in Escherichia coli. PLoS One 4:e6352. doi: 10.1371\/journal.pone.0006352","journal-title":"PLoS One"},{"key":"4071_CR6","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1111\/j.1467-7652.2009.00479.x","volume":"8","author":"A Davoodi-Semiromi","year":"2010","unstructured":"Davoodi-Semiromi A, Schreiber M, Nalapalli S, Verma D, Singh ND, Chakrabarti D, Daniell H (2010) Chloroplast-derived vaccine antigens confer dual immunity against cholera and malaria by oral or injectable delivery. Plant Biotech J 8:223\u2013242. doi: 10.1111\/j.1467-7652.2009.00479.x","journal-title":"Plant Biotech J"},{"key":"4071_CR7","doi-asserted-by":"crossref","first-page":"711","DOI":"10.4269\/ajtmh.1992.46.711","volume":"46","author":"PM Graves","year":"1992","unstructured":"Graves PM, Doubrovsky A, Sattabongkot J, Battistutta D (1992) Human antibody responses to epitopes on the Plasmodium falciparum gametocyte antigen Pfs 48\/45 and their relationship to infectivity of gametocyte carriers. AmJTrop Med Hyg 46:711\u2013719","journal-title":"AmJTrop Med Hyg"},{"key":"4071_CR8","doi-asserted-by":"crossref","first-page":"1600","DOI":"10.1111\/j.1469-0691.2011.03612.x","volume":"17","author":"BM Greenwood","year":"2011","unstructured":"Greenwood BM, Targett GAT (2011) Malaria vaccines and the new malaria agenda. Clin Microbiol Infect 17:1600\u20131607. doi: 10.1111\/j.1469-0691.2011.03612.x","journal-title":"Clin Microbiol Infect"},{"key":"4071_CR9","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.1172\/JCI33996","volume":"4","author":"BM Greenwood","year":"2008","unstructured":"Greenwood BM, Fidock DA, Kyle DE, Kappe SHI, Alonso PL, Collins FH, Duffy PE (2008) Malaria: progress, perils and prospects for eradication. J Clin Invest 4:1266\u20131276. doi: 10.1172\/JCI33996","journal-title":"J Clin Invest"},{"key":"4071_CR10","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/0166-6851(93)90158-T","volume":"61","author":"CHM Kocken","year":"1993","unstructured":"Kocken CHM, Jansen J, Kaan AM, Beckers PJA, Ponnudurai T, Kaslow DC, Konings RNH, Schoenmakers JGG (1993) Cloning and expression of the gene coding for the transmission blocking target antigen Pfs48\/45 of Plasmodium falciparum. Mol Biochem Parasitol 61:59\u201368. doi: 10.1016\/0166-6851(93)90158-T","journal-title":"Mol Biochem Parasitol"},{"key":"4071_CR11","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1111\/j.1467-7652.2007.00249.x","volume":"5","author":"AL Manuell","year":"2007","unstructured":"Manuell AL, Beligni MV, Elder JH, Siefker DT, Tran M, Weber A, McDonald TL, Mayfield SP (2007) Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast. Plant Biotech J 5:402\u2013412. doi: 10.1111\/j.1467-7652.2007.00249.x","journal-title":"Plant Biotech J"},{"key":"4071_CR12","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1073\/pnas.0237108100","volume":"100","author":"SP Mayfield","year":"2003","unstructured":"Mayfield SP, Franklin SE, Lerner RA (2003) Expression and assembly of a fully active antibody in algae. Proc Nat Acad Sci 100:438\u2013442. doi: 10.1073\/pnas.0237108100","journal-title":"Proc Nat Acad Sci"},{"key":"4071_CR13","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1006\/expr.1998.4315","volume":"90","author":"RLB Milek","year":"1998","unstructured":"Milek RLB, DeVries AAF, Roeffen WFG, Stunnenberg H, Rottier PJM, Konings NH (1998) Plasmodium falciparum: heterologous synthesis of the transmission-blocking vaccine candidate Pfs48\/45 in recombinant Vaccinia virus-infected cells. Exper Parasitol 90:165\u2013174. doi: 10.1006\/expr.1998.4315","journal-title":"Exper Parasitol"},{"key":"4071_CR14","doi-asserted-by":"crossref","first-page":"1402","DOI":"10.1016\/S0264-410X(99)00392-8","volume":"18","author":"RLB Milek","year":"2000","unstructured":"Milek RLB, Stunnenberg HG, Konings RNH (2000) Assembly and expression of a synthetic gene encoding the antigen Pfs48\/45 of the human malaria parasite Plasmodium falciparum in yeast. Vaccine 18:1402\u20131411. doi: 10.1016\/S0264-410X(99)00392-8","journal-title":"Vaccine"},{"key":"4071_CR15","doi-asserted-by":"crossref","first-page":"4957","DOI":"10.1128\/IAI.05288-11","volume":"79","author":"AL Ou\u00e9draogo","year":"2011","unstructured":"Ou\u00e9draogo AL, Roeffen W, Luty AJF, de Vlas SJ, Nebie I, Edith IS, Cuzin-Ouattara N, Teleen K, Tiono K, Tiono AB, Sirima SB, Verhave JP, Bousema T, Sauerwein R (2011) Naturally acquired immune responses to Plasmodium falciparum sexual stage antigens Pfs48\/45 and Pfs230 in an area of seasonal transmission. Infect Immun 79:4957\u20134964. doi: 10.1128\/IAI.05288-11","journal-title":"Infect Immun"},{"key":"4071_CR16","doi-asserted-by":"crossref","first-page":"17148","DOI":"10.1074\/jbc.M700948200","volume":"282","author":"N Outchkourov","year":"2007","unstructured":"Outchkourov N, Vermunt A, Jansen J, Kaan A, Roeffen W, Teelen K, Lasonder E, Braks A, van de Vegte-Bolmer M, Qiu LY, Sauerwein R, Stunnenberg HG (2007) Epitope analysis of the malaria surface antigen Pfs48\/45 identifies a subdomain that elicits transmission blocking antibodies. J Biol Chem 282:17148\u201317156. doi: 10.1074\/jbc.M700948200","journal-title":"J Biol Chem"},{"key":"4071_CR17","doi-asserted-by":"crossref","first-page":"4301","DOI":"10.1073\/pnas.0800459105","volume":"105","author":"NS Outchkourov","year":"2008","unstructured":"Outchkourov NS, Roeffen W, Kaan A, Jansen J, Luty A, Schuiffel D, van Gemert GJ, van de Vegte-Bolmer M, Sauerwein RW, Stunnenberg HG (2008) Correctly folded Pfs48\/45 protein of Plasmodium falciparum elicits malaria transmission-blocking immunity in mice. Proc Nat Acad Sci 105:4301\u20134305. doi: 10.1073\/pnas.0800459105","journal-title":"Proc Nat Acad Sci"},{"key":"4071_CR18","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1111\/j.1467-7652.2010.00503.x","volume":"8","author":"BA Rasala","year":"2010","unstructured":"Rasala BA, Muto M, Lee PA, Jager M, Cardoso RMF, Behnke CA, Kirk P, Hokanson CA, Crea R, Mendez M, Mayfield SP (2010) Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii. Plant Biotech J 8:719\u2013733. doi: 10.1111\/j.1467-7652.2010.00503.x","journal-title":"Plant Biotech J"},{"key":"4071_CR19","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1111\/j.1467-7652.2011.00620.x","volume":"9","author":"BA Rasala","year":"2011","unstructured":"Rasala BA, Muto M, Sullivan J, Mayfield SP (2011) Improved heterologous expression in the chloroplast of Chlamydomonas reinhardtii through promoter and 5\u2019 untranslated region optimization. Plant Biotech J 9:674\u2013683. doi: 10.1111\/j.1467-7652.2011.00620.x","journal-title":"Plant Biotech J"},{"key":"4071_CR20","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1084\/jem.158.3.976","volume":"158","author":"J Rener","year":"1983","unstructured":"Rener J, Graves PM, Carter R, Williams JL, Burkot TR (1983) Target antigens of transmission-blocking immunity on gametes of Plasmodium falciparum. J Exp Med 158:976\u2013981. doi: 10.1084\/jem.158.3.976","journal-title":"J Exp Med"},{"key":"4071_CR21","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1097\/QCO.0b013e3282a95e12","volume":"20","author":"A Saul","year":"2007","unstructured":"Saul A (2007) Mosquito stage, transmission blocking vaccines for malaria. Curr Opin Infect Dis 20:476\u2013481. doi: 10.1097\/QCO.0b013e3282a95e12","journal-title":"Curr Opin Infect Dis"},{"key":"4071_CR22","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/1475-2875-11-11","volume":"11","author":"L Schwartz","year":"2012","unstructured":"Schwartz L, Brown GV, Genton B, Moorthy VS (2012) A review of malaria vaccine clinical projects based on the WHO rainbow table. Malaria J 11:11. doi: 10.1186\/1475-2875-11-11","journal-title":"Malaria J"},{"key":"4071_CR23","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.molbiopara.2009.03.007","volume":"166","author":"CJ Sutherland","year":"2009","unstructured":"Sutherland CJ (2009) Surface antigens of Plasmodium falciparum gametocytes\u2014a new class of transmission-blocking vaccine targets? Mol Biochem Parasitol 166:93\u201398. doi: 10.1016\/j.molbiopara.2009.03.007","journal-title":"Mol Biochem Parasitol"},{"key":"4071_CR24","doi-asserted-by":"crossref","first-page":"S10","DOI":"10.1186\/1475-2875-7-S1-S10","volume":"7","author":"GA Targett","year":"2008","unstructured":"Targett GA, Greenwood B (2008) Malaria vaccines and their potential role in the elimination of malaria. Malaria J 7:S10. doi: 10.1186\/1475-2875-7-S1-S10","journal-title":"Malaria J"},{"key":"4071_CR25","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/0165-2478(90)90095-8","volume":"25","author":"GAT Targett","year":"1990","unstructured":"Targett GAT, Harte PG, Eida S, Rogers NC, Ong CSL (1990) Plasmodium falciparum sexual stage antigens: immunogenicity and cell-mediated responses. Immunol Let 25:77\u201382. doi: 10.1016\/0165-2478(90)90095-8","journal-title":"Immunol Let"},{"key":"4071_CR26","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1038\/nrmicro1712","volume":"5","author":"SM Todryk","year":"2007","unstructured":"Todryk SM, Hill AVS (2007) Malaria vaccines: the stage we are at. Nat Rev Microbiol 5:487\u2013489. doi: 10.1038\/nrmicro1712","journal-title":"Nat Rev Microbiol"},{"key":"4071_CR27","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1002\/bit.22446","volume":"104","author":"M Tran","year":"2009","unstructured":"Tran M, Zhou B, Pettersson PL, Gonzalez MJ, Mayfield SP (2009) Synthesis and assembly of a full-length human monoclonal antibody in algal chloroplasts. Biotech Bioeng 104:663\u2013673. doi: 10.1002\/bit.22446","journal-title":"Biotech Bioeng"},{"key":"4071_CR28","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/S0092-8674(01)00199-4","volume":"104","author":"MR Dijk Van","year":"2001","unstructured":"Van Dijk MR, Janse CJ, Thompson J, Waters AP, Braks JAM, Dodemont HJ, Stunnenberg HG, van Gemert GJ, Sauerwein RW, Eling W (2001) A central role for Pfs48\/45 in malaria parasite male gamete fertility. Cell 104:153\u2013164. doi: 10.1016\/S0092-8674(01)00199-4","journal-title":"Cell"},{"key":"4071_CR29","doi-asserted-by":"crossref","first-page":"e1000853","DOI":"10.1371\/journal.ppat.1000853","volume":"6","author":"MR Dijk Van","year":"2010","unstructured":"Van Dijk MR, van Schaijk BCL, Khan SM, van Dooren MW, Ramesar J, Kaczanowski S, van Gemert GJ, Kroeze H, Stunnenberg HG, Eling WM, Sauerwein RW, Waters AP, Janse CJ (2010) Three members of the 6-Cys protein family of Plasmodium play a role in gamete fertility. PLOS Pathogens 6:e1000853. doi: 10.1371\/journal.ppat.1000853","journal-title":"PLOS Pathogens"},{"key":"4071_CR30","doi-asserted-by":"crossref","first-page":"1460","DOI":"10.1084\/jem.162.5.1460","volume":"162","author":"AN Vermeulen","year":"1985","unstructured":"Vermeulen AN, Ponnudurai T, Beckers PJA, Verhave JP, Smits MA, Meuwissen JHE (1985) Sequential expression of antigens on sexual stages of Plasmodium falciparum accessible to transmission-blocking antibodies in the mosquito. J Exp Med 162:1460\u20131476. doi: 10.1084\/jem.162.5.1460","journal-title":"J Exp Med"},{"key":"4071_CR31","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1111\/j.1467-7652.2009.00447.x","volume":"7","author":"DE Webster","year":"2009","unstructured":"Webster DE, Wang L, Mulcair M, Ma C, Santi L, Mason HS, Wesselingh SL, Coppel RL (2009) Production and characterization of an orally immunogenic Plasmodium antigen in plants using a virus-based expression system. Plant Biotech J 7:846\u2013855. doi: 10.1111\/j.1467-7652.2009.00447.x","journal-title":"Plant Biotech J"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-012-4071-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00253-012-4071-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-012-4071-7","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T05:49:10Z","timestamp":1743140950000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00253-012-4071-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,5,18]]},"references-count":31,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2013,3]]}},"alternative-id":["4071"],"URL":"https:\/\/doi.org\/10.1007\/s00253-012-4071-7","relation":{},"ISSN":["0175-7598","1432-0614"],"issn-type":[{"value":"0175-7598","type":"print"},{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,5,18]]}}}