{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T21:41:23Z","timestamp":1789422083252,"version":"build-2803163510"},"reference-count":36,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2013,12,28]],"date-time":"2013-12-28T00:00:00Z","timestamp":1388188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2013,12,28]],"date-time":"2013-12-28T00:00:00Z","timestamp":1388188800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"published-print":{"date-parts":[[2014,4]]},"DOI":"10.1007\/s00253-013-5408-6","type":"journal-article","created":{"date-parts":[[2013,12,26]],"date-time":"2013-12-26T22:13:53Z","timestamp":1388096033000},"page":"3495-3507","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":79,"title":["Immunological features and efficacy of a multi-epitope vaccine CTB-UE against H. pylori in BALB\/c mice model"],"prefix":"10.1007","volume":"98","author":[{"given":"Le","family":"Guo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Runting","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunmei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobo","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonghong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangguo","family":"Duan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuankui","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Xi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingying","family":"Xing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,12,28]]},"reference":[{"issue":"6603","key":"5408_CR1","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1038\/383787a0","volume":"383","author":"AK Abbas","year":"1996","unstructured":"Abbas AK, Murphy KM, Sher A (1996) Functional diversity of helper T lymphocytes. Nature 383(6603):787\u2013793","journal-title":"Nature"},{"issue":"1","key":"5408_CR2","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.bbrc.2004.06.118","volume":"321","author":"AP Areas","year":"2004","unstructured":"Areas AP, Oliveira ML, Miyaji EN, Leite LC, Aires KA, Dias WO, Ho PL (2004) Expression and characterization of cholera toxin B-pneumococcal surface adhesin A fusion protein in Escherichia coli: ability of CTB-PsaA to induce humoral immune response in mice. Biochem Biophys Res Commun 321(1):192\u2013196","journal-title":"Biochem Biophys Res Commun"},{"key":"5408_CR3","doi-asserted-by":"crossref","first-page":"28","DOI":"10.2174\/1875035400902010028","volume":"2","author":"RE Begue","year":"2009","unstructured":"Begue RE, Moll A (2009) Immunogenicity of recombinant Helicobacter pylori urease B administered by various routes and with different adjuvants. Open Vaccine J 2:28\u201332","journal-title":"Open Vaccine J"},{"issue":"1","key":"5408_CR4","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1111\/j.1365-2036.2005.02521.x","volume":"22","author":"EA Cameron","year":"2005","unstructured":"Cameron EA, Bell GD (2005) Long-term follow-up of Helicobacter pylori eradication therapy in Vietnam: reinfection and clinical outcome. Aliment Pharmacol Ther 22(1):76\u201377","journal-title":"Aliment Pharmacol Ther"},{"issue":"8","key":"5408_CR5","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1086\/444425","volume":"192","author":"B Corthesy","year":"2005","unstructured":"Corthesy B, Boris S, Isler P, Grangette C, Mercenier A (2005) Oral immunization of mice with lactic acid bacteria producing Helicobacter pylori urease B subunit partially protects against challenge with Helicobacter felis. J Infect Dis 192(8):1441\u20131449","journal-title":"J Infect Dis"},{"issue":"3","key":"5408_CR6","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1111\/j.1523-5378.2011.00839.x","volume":"16","author":"ES DeLyria","year":"2011","unstructured":"DeLyria ES, Nedrud JG, Ernst PB, Alam MS, Redline RW, Ding H, Czinn SJ, Xu J, Blanchard TG (2011) Vaccine-induced immunity against Helicobacter pylori in the absence of IL-17A. Helicobacter 16(3):169\u2013178","journal-title":"Helicobacter"},{"issue":"1","key":"5408_CR7","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1053\/j.gastro.2008.09.017","volume":"136","author":"ES DeLyria","year":"2009","unstructured":"DeLyria ES, Redline RW, Blanchard TG (2009) Vaccination of mice against H. pylori induces a strong Th-17 response and immunity that is neutrophil dependent. Gastroenterology 136(1):247\u2013256","journal-title":"Gastroenterology"},{"issue":"12","key":"5408_CR8","doi-asserted-by":"crossref","first-page":"2277","DOI":"10.1084\/jem.188.12.2277","volume":"188","author":"TH Ermak","year":"1998","unstructured":"Ermak TH, Giannasca PJ, Nichols R, Myers GA, Nedrud J, Weltzin R, Lee CK, Kleanthous H, Monath TP (1998) Immunization of mice with urease vaccine affords protection against Helicobacter pylori infection in the absence of antibodies and is mediated by MHC class II-restricted responses. J Exp Med 188(12):2277\u20132288","journal-title":"J Exp Med"},{"issue":"4","key":"5408_CR9","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1128\/IAI.66.4.1349-1355.1998","volume":"66","author":"RL Ferrero","year":"1998","unstructured":"Ferrero RL, Thiberge JM, Huerre M, Labigne A (1998) Immune responses of specific-pathogen-free mice to chronic Helicobacter pylori (strain SS1) infection. Infect Immun 66(4):1349\u20131355","journal-title":"Infect Immun"},{"issue":"1","key":"5408_CR10","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/S0016-5085(97)70094-5","volume":"113","author":"RL Ferrero","year":"1997","unstructured":"Ferrero RL, Thiberge JM, Labigne A (1997) Local immunoglobulin G antibodies in the stomach may contribute to immunity against Helicobacter infection in mice. Gastroenterology 113(1):185\u2013194","journal-title":"Gastroenterology"},{"issue":"4","key":"5408_CR11","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1002\/bit.20060","volume":"86","author":"R Fujii","year":"2004","unstructured":"Fujii R, Morihara F, Oku T, Hifumi E, Uda T (2004) Epitope mapping and features of the epitope for monoclonal antibodies inhibiting enzymatic activity of Helicobacter pylori urease. Biotechnol Bioeng 86(4):434\u2013444","journal-title":"Biotechnol Bioeng"},{"issue":"5","key":"5408_CR12","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/S0264-410X(97)00247-8","volume":"16","author":"OG Gomez-Duarte","year":"1998","unstructured":"Gomez-Duarte OG, Lucas B, Yan ZX, Panthel K, Haas R, Meyer TF (1998) Protection of mice against gastric colonization by Helicobacter pylori by single oral dose immunization with attenuated Salmonella typhimurium producing urease subunits A and B. Vaccine 16(5):460\u2013471","journal-title":"Vaccine"},{"issue":"5","key":"5408_CR13","doi-asserted-by":"crossref","first-page":"1937","DOI":"10.1007\/s00253-011-3726-0","volume":"93","author":"L Guo","year":"2012a","unstructured":"Guo L, Li X, Tang F, He Y, Xing Y, Deng X, Xi T (2012a) Immunological features and the ability of inhibitory effects on enzymatic activity of an epitope vaccine composed of cholera toxin B subunit and B cell epitope from Helicobacter pylori urease A subunit. Appl Microbiol Biotechnol 93(5):1937\u20131945","journal-title":"Appl Microbiol Biotechnol"},{"issue":"6","key":"5408_CR14","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1007\/s00253-012-4122-0","volume":"95","author":"L Guo","year":"2012b","unstructured":"Guo L, Liu K, Xu G, Li X, Tu J, Tang F, Xing Y, Xi T (2012b) Prophylactic and therapeutic efficacy of the epitope vaccine CTB-UA against Helicobacter pylori infection in a BALB\/c mice model. Appl Microbiol Biotechnol 95(6):1437\u20131444","journal-title":"Appl Microbiol Biotechnol"},{"issue":"6","key":"5408_CR15","doi-asserted-by":"crossref","first-page":"2367","DOI":"10.1007\/s00253-012-4486-1","volume":"97","author":"L Guo","year":"2013","unstructured":"Guo L, Liu K, Zhao W, Li X, Li T, Tang F, Zhang R, Wu W, Xi T (2013) Immunological features and efficacy of the reconstructed epitope vaccine CtUBE against Helicobacter pylori infection in BALB\/c mice model. Appl Microbiol Biotechnol 97(6):2367\u20132378","journal-title":"Appl Microbiol Biotechnol"},{"key":"5408_CR16","doi-asserted-by":"crossref","unstructured":"Guy B, Hessler C, Fourage S, Haensler J, Vialon-Lafay E, Rokbi B, Millet MJ (1998) Systemic immunization with urease protects mice against Helicobacter pylori infection. Vaccine 16(8):850\u2013856","DOI":"10.1016\/S0264-410X(97)00258-2"},{"issue":"1","key":"5408_CR17","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.imlet.2005.10.016","volume":"103","author":"E Hifumi","year":"2006","unstructured":"Hifumi E, Yamada Y, Uda T (2006) A catalytic antibody heavy chain HpU-2 degrading its epitope peptide and H. pylori urease. Immunol Lett 103(1):68\u201374","journal-title":"Immunol Lett"},{"issue":"5","key":"5408_CR18","doi-asserted-by":"crossref","first-page":"183","DOI":"10.7883\/yoken.52.183","volume":"52","author":"Y Hirai","year":"1999","unstructured":"Hirai Y, Hayashi S, Shimomura H, Oguma K, Yokota K (1999) Association of Helicobacter pylori with gastroduodenal diseases. Jpn J Infect Dis 52(5):183\u2013197","journal-title":"Jpn J Infect Dis"},{"issue":"11","key":"5408_CR19","doi-asserted-by":"crossref","first-page":"6597","DOI":"10.1128\/IAI.69.11.6597-6603.2001","volume":"69","author":"K Hirota","year":"2001","unstructured":"Hirota K, Nagata K, Norose Y, Futagami S, Nakagawa Y, Senpuku H, Kobayashi M, Takahashi H (2001) Identification of an antigenic epitope in Helicobacter pylori urease that induces neutralizing antibody production. Infect Immun 69(11):6597\u20136603","journal-title":"Infect Immun"},{"issue":"1","key":"5408_CR20","first-page":"80","volume":"21","author":"MK Khan","year":"2012","unstructured":"Khan MK, Bemana M (2012) Association of Helicobacter pylori infection and gastric carcinoma. Mymensingh Med J 21(1):80\u201384","journal-title":"Mymensingh Med J"},{"issue":"12","key":"5408_CR21","doi-asserted-by":"crossref","first-page":"1884","DOI":"10.1016\/j.bbagen.2006.07.015","volume":"1760","author":"J Kovacs-Nolan","year":"2006","unstructured":"Kovacs-Nolan J, Mine Y (2006) Tandem copies of a human rotavirus VP8 epitope can induce specific neutralizing antibodies in BALB\/c mice. Biochim Biophys Acta 1760(12):1884\u20131893","journal-title":"Biochim Biophys Acta"},{"issue":"1","key":"5408_CR22","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1093\/infdis\/172.1.161","volume":"172","author":"CK Lee","year":"1995","unstructured":"Lee CK, Weltzin R, Thomas WD Jr, Kleanthous H, Ermak TH, Soman G, Hill JE, Ackerman SK, Monath TP (1995) Oral immunization with recombinant Helicobacter pylori urease induces secretory IgA antibodies and protects mice from challenge with Helicobacter felis. J Infect Dis 172(1):161\u2013172","journal-title":"J Infect Dis"},{"issue":"3","key":"5408_CR23","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1016\/j.vaccine.2004.05.028","volume":"23","author":"W Liu","year":"2004","unstructured":"Liu W, Peng Z, Liu Z, Lu Y, Ding J, Chen YH (2004) High epitope density in a single recombinant protein molecule of the extracellular domain of influenza A virus M2 protein significantly enhances protective immunity. Vaccine 23(3):366\u2013371","journal-title":"Vaccine"},{"issue":"3","key":"5408_CR24","doi-asserted-by":"crossref","first-page":"1714","DOI":"10.1128\/IAI.69.3.1714-1721.2001","volume":"69","author":"B Lucas","year":"2001","unstructured":"Lucas B, Bumann D, Walduck A, Koesling J, Develioglu L, Meyer TF, Aebischer T (2001) Adoptive transfer of CD4+ T cells specific for subunit A of Helicobacter pylori urease reduces H. pylori stomach colonization in mice in the absence of interleukin-4 (IL-4)\/IL-13 receptor signaling. Infect Immun 69(3):1714\u20131721","journal-title":"Infect Immun"},{"issue":"1","key":"5408_CR25","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1136\/gutjnl-2012-302254","volume":"62","author":"F Megraud","year":"2013","unstructured":"Megraud F, Coenen S, Versporten A, Kist M, Lopez-Brea M, Hirschl AM, Andersen LP, Goossens H, Glupczynski Y (2013) Helicobacter pylori resistance to antibiotics in Europe and its relationship to antibiotic consumption. Gut 62(1):34\u201342","journal-title":"Gut"},{"issue":"1","key":"5408_CR26","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1111\/j.1574-6968.2002.tb11416.x","volume":"216","author":"CA Menezes","year":"2002","unstructured":"Menezes CA, Amianti J, Harayama HS, Koga PC, Trabulsi LR, Piazza RM (2002) Inhibition of Escherichia coli heat-labile enterotoxin by neoglycoprotein and anti-lectin antibodies which mimic GM1 receptor. FEMS Microbiol Lett 216(1):67\u201370","journal-title":"FEMS Microbiol Lett"},{"issue":"6","key":"5408_CR27","doi-asserted-by":"crossref","first-page":"1848","DOI":"10.1016\/S0016-5085(97)70004-0","volume":"113","author":"M Mohammadi","year":"1997","unstructured":"Mohammadi M, Nedrud J, Redline R, Lycke N, Czinn SJ (1997) Murine CD4 T-cell response to Helicobacter infection: TH1 cells enhance gastritis and TH2 cells reduce bacterial load. Gastroenterology 113(6):1848\u20131857","journal-title":"Gastroenterology"},{"issue":"11","key":"5408_CR28","doi-asserted-by":"crossref","first-page":"4826","DOI":"10.1128\/iai.60.11.4826-4831.1992","volume":"60","author":"K Nagata","year":"1992","unstructured":"Nagata K, Mizuta T, Tonokatu Y, Fukuda Y, Okamura H, Hayashi T, Shimoyama T, Tamura T (1992) Monoclonal antibodies against the native urease of Helicobacter pylori: synergistic inhibition of urease activity by monoclonal antibody combinations. Infect Immun 60(11):4826\u20134831","journal-title":"Infect Immun"},{"issue":"14","key":"5408_CR29","doi-asserted-by":"crossref","first-page":"2591","DOI":"10.1016\/j.vaccine.2006.12.026","volume":"25","author":"J Nystrom","year":"2007","unstructured":"Nystrom J, Svennerholm AM (2007) Oral immunization with HpaA affords therapeutic protective immunity against H. pylori that is reflected by specific mucosal immune responses. Vaccine 25(14):2591\u20132598","journal-title":"Vaccine"},{"issue":"32","key":"5408_CR30","doi-asserted-by":"crossref","first-page":"5220","DOI":"10.1016\/j.vaccine.2010.05.067","volume":"28","author":"Y Qiu","year":"2010","unstructured":"Qiu Y, Wang YC, Tao HX, Zhan DW, Yuan SL, Wang P, Wang LC, Han XP, Li CS, Li JK, Liu CJ (2010) Identification of B-cell epitopes in urease B subunit of Helicobacter pylori bound by neutralizing antibodies. Vaccine 28(32):5220\u20135227","journal-title":"Vaccine"},{"issue":"11","key":"5408_CR31","doi-asserted-by":"crossref","first-page":"7085","DOI":"10.4049\/jimmunol.0803293","volume":"182","author":"A Sayi","year":"2009","unstructured":"Sayi A, Kohler E, Hitzler I, Arnold I, Schwendener R, Rehrauer H, Muller A (2009) The CD4+ T cell-mediated IFN-gamma response to Helicobacter infection is essential for clearance and determines gastric cancer risk. J Immunol 182(11):7085\u20137101","journal-title":"J Immunol"},{"issue":"14","key":"5408_CR32","doi-asserted-by":"crossref","first-page":"2583","DOI":"10.1016\/j.vaccine.2006.12.024","volume":"25","author":"Y Shi","year":"2007","unstructured":"Shi Y, Wu C, Zhou WY, Mao XH, Guo G, Zou QM (2007) Identification of H-2d restricted Th epitopes in Urease B subunit of Helicobacter pylori. Vaccine 25(14):2583\u20132590","journal-title":"Vaccine"},{"issue":"5","key":"5408_CR33","first-page":"9","volume":"87","author":"S Tsimmerman Ia","year":"2009","unstructured":"Tsimmerman Ia S (2009) Gastroduodenal diseases and Helicobacter pylori infection: overview. Klin Med (Mosk) 87(5):9\u201315","journal-title":"Klin Med (Mosk)"},{"issue":"32","key":"5408_CR34","doi-asserted-by":"crossref","first-page":"3250","DOI":"10.1016\/j.vaccine.2013.05.047","volume":"31","author":"M Vermoote","year":"2013","unstructured":"Vermoote M, Flahou B, Pasmans F, Ducatelle R, Haesebrouck F (2013) Protective efficacy of vaccines based on the Helicobacter suis urease subunit B and gamma-glutamyl transpeptidase. Vaccine 31(32):3250\u20133256","journal-title":"Vaccine"},{"issue":"44","key":"5408_CR35","doi-asserted-by":"crossref","first-page":"7664","DOI":"10.1016\/j.vaccine.2007.08.034","volume":"25","author":"W Zhao","year":"2007","unstructured":"Zhao W, Wu W, Xu X (2007) Oral vaccination with liposome-encapsulated recombinant fusion peptide of urease B epitope and cholera toxin B subunit affords prophylactic and therapeutic effects against H. pylori infection in BALB\/c mice. Vaccine 25(44):7664\u20137673","journal-title":"Vaccine"},{"issue":"36","key":"5408_CR36","doi-asserted-by":"crossref","first-page":"5013","DOI":"10.1016\/j.vaccine.2009.05.009","volume":"27","author":"WY Zhou","year":"2009","unstructured":"Zhou WY, Shi Y, Wu C, Zhang WJ, Mao XH, Guo G, Li HX, Zou QM (2009) Therapeutic efficacy of a multi-epitope vaccine against Helicobacter pylori infection in BALB\/c mice model. Vaccine 27(36):5013\u20135019","journal-title":"Vaccine"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-013-5408-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00253-013-5408-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-013-5408-6","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-013-5408-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T17:06:46Z","timestamp":1744132006000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00253-013-5408-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12,28]]},"references-count":36,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["5408"],"URL":"https:\/\/doi.org\/10.1007\/s00253-013-5408-6","relation":{},"ISSN":["0175-7598","1432-0614"],"issn-type":[{"value":"0175-7598","type":"print"},{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12,28]]},"assertion":[{"value":"16 October 2013","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 November 2013","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 November 2013","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 December 2013","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}]}}