{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T13:27:51Z","timestamp":1773408471489,"version":"3.50.1"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"16","license":[{"start":{"date-parts":[[2014,4,12]],"date-time":"2014-04-12T00:00:00Z","timestamp":1397260800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"published-print":{"date-parts":[[2014,8]]},"DOI":"10.1007\/s00253-014-5728-1","type":"journal-article","created":{"date-parts":[[2014,4,11]],"date-time":"2014-04-11T20:23:30Z","timestamp":1397247810000},"page":"6973-6982","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Expression and purification of cyto-insectotoxin (Cit1a) using silkworm larvae targeting for an antimicrobial therapeutic agent"],"prefix":"10.1007","volume":"98","author":[{"given":"M. P.","family":"Ali","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuhiko","family":"Yoshimatsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomohiro","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuya","family":"Kato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enoch Y.","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2014,4,12]]},"reference":[{"key":"5728_CR1","doi-asserted-by":"crossref","first-page":"7411","DOI":"10.1016\/j.vaccine.2012.09.077","volume":"30","author":"SV Avilov","year":"2013","unstructured":"Avilov SV, Moisy D, Naffakh N, Cusack S (2013) Influenza A virus progeny vRNP trafficking in live infected cells studied with the virus-encoded fluorescently tagged PB2 protein. Vaccine 30:7411\u20137417","journal-title":"Vaccine"},{"issue":"Suppl 4","key":"5728_CR2","doi-asserted-by":"crossref","first-page":"S258","DOI":"10.1086\/430786","volume":"41","author":"MA Aziz","year":"2005","unstructured":"Aziz MA, Wright A (2005) The World Health Organization\/International Union against tuberculosis and lung disease global project on surveillance for anti-tuberculosis drug resistance: a model for other infectious diseases. Clin Infect Dis 41(Suppl 4):S258\u2013S262","journal-title":"Clin Infect Dis"},{"key":"5728_CR3","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1126\/science.8303295","volume":"263","author":"M Chalfie","year":"1994","unstructured":"Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasherf DC (1994) Green fluorescent protein as a marker for gene expression. Science 263:802\u2013804","journal-title":"Science"},{"key":"5728_CR4","doi-asserted-by":"crossref","first-page":"3683","DOI":"10.1128\/AAC.00251-09","volume":"53","author":"X Chen","year":"2009","unstructured":"Chen X, Zhu FM, Cao YH, Qiao SY (2009) Novel expression vector for secretion of cecropin AD in Bacillus subtilis with enhanced antimicrobial activity. Antimicrob Agents Chemother 53:3683\u20133689","journal-title":"Antimicrob Agents Chemother"},{"key":"5728_CR5","volume-title":"Method for antifungal disk diffusion susceptibility testing of filamentous fungi; proposed guideline. CLSI document M51-P","author":"CLSI","year":"2009","unstructured":"CLSI (2009) Method for antifungal disk diffusion susceptibility testing of filamentous fungi; proposed guideline. CLSI document M51-P. Clinical and Laboratory Standards Institute, Wayne"},{"key":"5728_CR6","doi-asserted-by":"crossref","unstructured":"Deo VK, Yui M, Alam MJ, Yamazaki M, Kato T, Park EY (2014) A model for targeting colon carcinoma cells using single-chain variable fragments anchored on virus-like particles via glycosyl phosphatidylinositol anchor. Pharm Res. 10.1007\/s11095-014-1316-4","DOI":"10.1007\/s11095-014-1316-4"},{"key":"5728_CR7","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1007\/BF01640803","volume":"9","author":"H Dickert","year":"1981","unstructured":"Dickert H, Machka K, Braveny I (1981) The uses and limitations of disc diffusion in the antibiotic sensitivity testing of bacteria. Infection 9:18\u201324","journal-title":"Infection"},{"key":"5728_CR8","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.pep.2010.05.012","volume":"73","author":"F Fan","year":"2010","unstructured":"Fan F, Wu Y, Liu J (2010) Expression and purification of two different antimicrobial peptides, PR-39 and Protegrin-1 in Escherichia coli. Protein Express Purif 73:147\u2013151","journal-title":"Protein Express Purif"},{"key":"5728_CR9","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1128\/AAC.01030-06","volume":"51","author":"LF Fehri","year":"2007","unstructured":"Fehri LF, Wroblewski H, Blanchard A (2007) Activities of antimicrobial peptides and synergy with enrofloxacin against Mycoplasma pulmonis. Antimicrob Agents Chemother 51:468\u2013474","journal-title":"Antimicrob Agents Chemother"},{"key":"5728_CR10","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1080\/10826068.2012.762717","volume":"43","author":"M Fukushima","year":"2013","unstructured":"Fukushima M, Iiyama K, Yamashita J, Furue M, Tsuji G, Imaishi S, Mon H, Lee JM, Kusakabe T (2013) Production of small antibacterial peptides using silkworm-baculovirus protein expression. Prep Biochem Biotechnol 43:565\u2013576","journal-title":"Prep Biochem Biotechnol"},{"key":"5728_CR11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2478\/s11535-007-0010-5","volume":"2","author":"A Giuliani","year":"2007","unstructured":"Giuliani A, Pirri G, Nicoletto SF (2007) Antimicrobial peptides: an overview of a promising class of therapeutics. Cent Eur J Biol 2:1\u201333","journal-title":"Cent Eur J Biol"},{"key":"5728_CR12","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1038\/nbt1267","volume":"24","author":"RE Hancock","year":"2006","unstructured":"Hancock RE, Sahl HG (2006) Antimicrobial and host-defense peptides as new anti infective therapeutic strategies. Nat Biotechnol 24:1551\u20131557","journal-title":"Nat Biotechnol"},{"key":"5728_CR13","doi-asserted-by":"crossref","first-page":"2452","DOI":"10.1128\/AAC.06299-11","volume":"56","author":"K Hayakawa","year":"2012","unstructured":"Hayakawa K, Marchaim D, Martin ET, Tiwari N, Yusuf A, Sunkara B, Pulluru H, Kotra H, Hsana A, Bheemreddy S, Sheth P, Lee DW, Kamatan S, Bathima P, Nanjireddy P, Chalana IK, Patel S, Kumar S, Vahia A, Ku K, Yee V, Swan J, Pogue JM, Lephart PR, Rybak MJ, Kaye KS (2012) Comparison of the clinical characteristics and outcomes associated with vancomycin-resistant Enterococcus faecalis and vancomycin-resistant E. faecium bacteremia. Antimicrob Agents Chemother 56:2452\u20132458","journal-title":"Antimicrob Agents Chemother"},{"key":"5728_CR14","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.jviromet.2007.04.005","volume":"144","author":"M Hiyoshi","year":"2007","unstructured":"Hiyoshi M, Kageshima A, Kato T, Park EY (2007) Construction of a cysteine protease deficient Bombyx mori multiple nucleopolyhedrovirus bacmid and its application to improve expression of a fusion protein. J Virol Methods 144:91\u201397","journal-title":"J Virol Methods"},{"key":"5728_CR15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1042\/BA20060207","volume":"47","author":"AB Ingham","year":"2007","unstructured":"Ingham AB, Moore RJ (2007) Recombinant production of antimicrobial peptides in heterologous microbial systems. Biotechnol Appl Biochem 47:1\u20139","journal-title":"Biotechnol Appl Biochem"},{"key":"5728_CR16","unstructured":"Jiang L (2011) Comparison of disk diffusion, agar dilution, and broth microdilution for antimicrobial susceptibility testing of five chitosans. MSc Thesis, Louisiana State University and Agricultural and Mechanical College, USA"},{"key":"5728_CR17","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1007\/s00253-009-2267-2","volume":"85","author":"T Kato","year":"2010","unstructured":"Kato T, Kajikawa M, Maenaka K, Park EY (2010) Silkworm expression system as a platform technology in life science. Appl Microbiol Biot 85:459\u2013470","journal-title":"Appl Microbiol Biot"},{"key":"5728_CR18","doi-asserted-by":"crossref","first-page":"273","DOI":"10.4162\/nrp.2007.1.4.273","volume":"1","author":"JS Kim","year":"2007","unstructured":"Kim JS, Kim YH (2007) The inhibitory effect of natural bioactives on the growth of pathogenic bacteria. Nutr Res Pract 1:273\u2013278","journal-title":"Nutr Res Pract"},{"key":"5728_CR19","doi-asserted-by":"crossref","first-page":"20983","DOI":"10.1074\/jbc.M602168200","volume":"281","author":"SA Kozlov","year":"2006","unstructured":"Kozlov SA, Vassilevski AA, Feofanov AV, Surovoy AY, Karpunin DV, Grishin EV (2006) Latarcins, antimicrobial and cytolytic peptides from the venom of the spider Lachesana tarabaevi (Zodariidae) that exemplify biomolecular diversity. J Biol Chem 281:20983\u201320992","journal-title":"J Biol Chem"},{"key":"5728_CR20","doi-asserted-by":"crossref","first-page":"58","DOI":"10.2174\/187221308783399261","volume":"2","author":"SA Kozlov","year":"2008","unstructured":"Kozlov SA, Vassilevski AA, Grishin EV (2008) Antimicrobial peptide precursor structures suggest effective production strategies. Recent Pat Inflamm Allergy Drug Discov 2:58\u201363","journal-title":"Recent Pat Inflamm Allergy Drug Discov"},{"key":"5728_CR21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/B978-0-12-387668-3.00001-5","volume":"40","author":"L Kuhn-Nentwig","year":"2011","unstructured":"Kuhn-Nentwig L, Stocklin R, Nentwig W (2011) Venom composition and strategies in spiders: is everything possible? Adv Insect Physiol 40:1\u201386","journal-title":"Adv Insect Physiol"},{"key":"5728_CR22","doi-asserted-by":"crossref","first-page":"5367","DOI":"10.1128\/AAC.00449-11","volume":"55","author":"VN Lazarev","year":"2011","unstructured":"Lazarev VN, Polin NF, Shkarupeta MM, Kostrjukova ES, Vassilevski AA, Kozlov SA, Grishin EV, Govorun VM (2011) Spider venom peptides for gene therapy of Chlamydia infection. Antimicrob Agents Chemother 55:5367\u20135369","journal-title":"Antimicrob Agents Chemother"},{"key":"5728_CR23","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1007\/s00203-012-0863-5","volume":"195","author":"VN Lazarev","year":"2013","unstructured":"Lazarev VN, Shkarupeta MM, Polina NF, Kostrjukova ES, Vassilevski AA, Kozlov SA, Grishin EV, Govorun VM (2013) Antimicrobial peptide from spider venom inhibits Chlamydia trachomatis infection at an early stage. Arch Microbiol 195:173\u2013179","journal-title":"Arch Microbiol"},{"key":"5728_CR24","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1586\/14789450.5.5.731","volume":"5","author":"S Liang","year":"2008","unstructured":"Liang S (2008) Proteome and peptidome profiling of spider venoms. Expert Rev Proteomics 5:731\u2013746","journal-title":"Expert Rev Proteomics"},{"key":"5728_CR25","doi-asserted-by":"crossref","first-page":"1492","DOI":"10.3390\/md11051492","volume":"11","author":"Y Liu","year":"2013","unstructured":"Liu Y, Chen Y, Chen J, Zhang W, Sheng Q, Chen J, Yu W, Nie Z, Zhang Y, Wu W, Wang L, Indran IR, Li J, Qian L, Lv Z (2013) A shark liver gene-derived active peptide expressed in the silkworm, Bombyx mori: preliminary studies for oral administration of the recombinant protein. Mar Drugs 11:1492\u20131505","journal-title":"Mar Drugs"},{"key":"5728_CR26","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.foodres.2009.09.006","volume":"43","author":"P Mayachiew","year":"2010","unstructured":"Mayachiew P, Devahastin S, Mackey BM, Niranjan K (2010) Effects of drying methods and conditions on antimicrobial activity of edible chitosan films enriched with galangal extract. Food Res Int 43:125\u2013132","journal-title":"Food Res Int"},{"key":"5728_CR27","doi-asserted-by":"crossref","first-page":"2149","DOI":"10.1021\/ja00897a025","volume":"85","author":"RB Merrifield","year":"1963","unstructured":"Merrifield RB (1963) Solid phase peptide synthesis. The synthesis of a tetrapeptide. J Am Chem Soc 85:2149","journal-title":"J Am Chem Soc"},{"key":"5728_CR28","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/2193-1801-1-29","volume":"1","author":"M Palaniyandi","year":"2013","unstructured":"Palaniyandi M, Kato T, Park EY (2013) Expression of human papillomavirus 6b L1 protein in silkworm larvae and enhanced green fluorescent protein displaying on its virus-like particles. Springer Plus 1:29","journal-title":"Springer Plus"},{"key":"5728_CR29","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1016\/j.jbiotec.2007.01.028","volume":"129","author":"EY Park","year":"2007","unstructured":"Park EY, Kageshima A, Kwon MS, Kato T (2007) Enhanced production of secretory beta1,3-N-acetylglucosaminyltransferase 2 fusion protein into hemolymph of Bombyx mori larvae using recombinant BmNPV bacmid integrated signal sequence. J Biotechnol 129:681\u2013688","journal-title":"J Biotechnol"},{"key":"5728_CR30","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1007\/s12602-012-9108-9","volume":"4","author":"NF Polina","year":"2012","unstructured":"Polina NF, Shkarupeta MM, Popenko AS, Vassilevski AA, Kozlov SA, Grishin EV, Lazarev VN, Govorun VM (2012) Cyto-insectotoxin 1a from Lachesana tarabaevi spider venom inhibits Chlamydia trachomatis infection. Probiotics Antimicrob Proteins 4:208\u2013216","journal-title":"Probiotics Antimicrob Proteins"},{"key":"5728_CR31","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1002\/btpr.1650","volume":"29","author":"R Ramos","year":"2013","unstructured":"Ramos R, Moreira S, Rodrigues A, Gama M, Domingues L (2013) Recombinant expression and purification of the antimicrobial peptide Magainin-2. Biotechnol Prog 29:17\u201322","journal-title":"Biotechnol Prog"},{"key":"5728_CR32","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.visres.2013.10.008","volume":"93","author":"JD Sammons","year":"2013","unstructured":"Sammons JD, Gross AK (2013) Biochemical analysis of arhodopsin photoactivatable GFP fusion as a model of G-protein coupled receptor transport. Vision Res 93:43\u201348","journal-title":"Vision Res"},{"key":"5728_CR33","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1007\/s10529-010-0498-z","volume":"33","author":"M Tomita","year":"2011","unstructured":"Tomita M (2011) Transgenic silkworms that weave recombinant proteins in silk cocoons. Biotechnol Lett 33:645\u2013654","journal-title":"Biotechnol Lett"},{"key":"5728_CR34","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1042\/BJ20071123","volume":"411","author":"AA Vassilevski","year":"2008","unstructured":"Vassilevski AA, Kozov SA, Samsonova OV, Egorova NS, Karpunin DV, Pluznikov KA, Feofanov AV, Grishin EV (2008) Cyto-insectotoxins, a novel class of cytolytic and insecticidal peptides from spider venom. Biochem J 411:687\u2013696","journal-title":"Biochem J"},{"key":"5728_CR35","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1134\/S0006297909130069","volume":"74","author":"AA Vassilevski","year":"2009","unstructured":"Vassilevski AA, Kozlov SA, Grishin EV (2009) Molecular diversity of spider venom. Biochemistry (Mosc) 74:1505\u20131534","journal-title":"Biochemistry (Mosc)"},{"key":"5728_CR36","doi-asserted-by":"crossref","first-page":"2121","DOI":"10.1007\/s10529-011-0687-4","volume":"33","author":"Q Wang","year":"2011","unstructured":"Wang Q, Zhu F, Xin Y, Liu J, Luo L, Yin Z (2011) Expression and purification of antimicrobial peptide buforin IIb in Escherichia coli. Biotechnol Lett 33:2121\u20132126","journal-title":"Biotechnol Lett"},{"key":"5728_CR37","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1128\/AAC.3.3.350","volume":"3","author":"TD Wilkins","year":"1973","unstructured":"Wilkins TD, Thiel T (1973) Modified broth-disk method for testing the antibiotic susceptibility of anaerobic bacteria. Antimicrob Agents Chemother 3:350\u2013356","journal-title":"Antimicrob Agents Chemother"},{"key":"5728_CR38","doi-asserted-by":"crossref","first-page":"191","DOI":"10.3390\/toxins4030191","volume":"4","author":"MJ Windley","year":"2012","unstructured":"Windley MJ, Herzig V, Dziemborowicz SA, Hardy MC, King GF, Nicholson GM (2012) Speder-venom peptides as bioinsecticides. Toxins (Basel) 4:191\u2013227","journal-title":"Toxins (Basel)"},{"key":"5728_CR39","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1038\/nrmicro1614","volume":"5","author":"GD Wright","year":"2007","unstructured":"Wright GD (2007) The antibiotic resistome: the nexus of chemical and genetic diversity. Nat Rev Microbiol 5:175\u2013186","journal-title":"Nat Rev Microbiol"},{"key":"5728_CR40","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1128\/IAI.64.3.709-713.1996","volume":"64","author":"B Yasin","year":"1996","unstructured":"Yasin B, Harwig SL, Lehrer RI, Wagar EA (1996) Susceptibility of Chlamydia trachomatis to protegrins and defensins. Infect Immun 64:709\u2013713","journal-title":"Infect Immun"},{"key":"5728_CR41","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1124\/pr.55.1.2","volume":"55","author":"MR Yeaman","year":"2003","unstructured":"Yeaman MR, Yount NY (2003) Mechanisms of antimicrobial peptide action and resistance. Pharmacol Rev 55:27\u201355","journal-title":"Pharmacol Rev"},{"key":"5728_CR42","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1146\/annurev-pharmtox-010611-134535","volume":"52","author":"NY Yount","year":"2012","unstructured":"Yount NY, Yeaman MR (2012) Emerging themes and therapeutic prospects for anti-infective peptides. Annu Rev Pharmacol Toxicol 52:337\u2013360","journal-title":"Annu Rev Pharmacol Toxicol"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-014-5728-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00253-014-5728-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-014-5728-1","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,17]],"date-time":"2020-08-17T16:58:33Z","timestamp":1597683513000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00253-014-5728-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4,12]]},"references-count":42,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["5728"],"URL":"https:\/\/doi.org\/10.1007\/s00253-014-5728-1","relation":{},"ISSN":["0175-7598","1432-0614"],"issn-type":[{"value":"0175-7598","type":"print"},{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,4,12]]}}}