{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T17:24:01Z","timestamp":1793294641155,"version":"4.5.0"},"reference-count":54,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2014,7,20]],"date-time":"2014-07-20T00:00:00Z","timestamp":1405814400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"published-print":{"date-parts":[[2015,1]]},"DOI":"10.1007\/s00253-014-5930-1","type":"journal-article","created":{"date-parts":[[2014,7,19]],"date-time":"2014-07-19T00:23:56Z","timestamp":1405729436000},"page":"741-752","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":75,"title":["Biochemical and biophysical characterization of PlyGRCS, a bacteriophage endolysin active against methicillin-resistant Staphylococcus aureus"],"prefix":"10.1007","volume":"99","author":[{"given":"Sara B.","family":"Linden","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan D.","family":"Heselpoth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias","family":"Schmelcher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fritz","family":"Eichenseher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel C.","family":"Nelson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2014,7,20]]},"reference":[{"issue":"1","key":"5930_CR1","doi-asserted-by":"publisher","first-page":"46","DOI":"10.4161\/bact.1.1.13980","volume":"1","author":"ST Abedon","year":"2011","unstructured":"Abedon ST (2011) Lysis from without. Bacteriophage 1(1):46\u201349. doi: 10.4161\/bact.1.1.13980","journal-title":"Bacteriophage"},{"issue":"44","key":"5930_CR2","doi-asserted-by":"publisher","first-page":"18991","DOI":"10.1073\/pnas.1004304107","volume":"107","author":"ML Atilano","year":"2010","unstructured":"Atilano ML, Pereira PM, Yates J, Reed P, Veiga H, Pinho MG, Filipe SR (2010) Teichoic acids are temporal and spatial regulators of peptidoglycan cross-linking in Staphylococcus aureus. Proc Natl Acad Sci U S A 107(44):18991\u201318996. doi: 10.1073\/pnas.1004304107","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5","key":"5930_CR3","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1016\/S0968-0004(03)00061-6","volume":"28","author":"A Bateman","year":"2003","unstructured":"Bateman A, Rawlings ND (2003) The CHAP domain: a large family of amidases including GSP amidase and peptidoglycan hydrolases. Trends Biochem Sci 28(5):234\u2013237. doi: 10.1016\/S0968-0004(03)00061-6","journal-title":"Trends Biochem Sci"},{"issue":"2","key":"5930_CR4","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1111\/j.1574-6968.2008.01308.x","volume":"287","author":"SC Becker","year":"2008","unstructured":"Becker SC, Foster-Frey J, Donovan DM (2008) The phage K lytic enzyme LysK and lysostaphin act synergistically to kill MRSA. FEMS Microbiol Lett 287(2):185\u2013191. doi: 10.1111\/j.1574-6968.2008.01308.x","journal-title":"FEMS Microbiol Lett"},{"issue":"1","key":"5930_CR5","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1111\/j.1574-6968.2009.01541.x","volume":"294","author":"SC Becker","year":"2009","unstructured":"Becker SC, Dong S, Baker JR, Foster-Frey J, Pritchard DG, Donovan DM (2009) LysK CHAP endopeptidase domain is required for lysis of live staphylococcal cells. FEMS Microbiol Lett 294(1):52\u201360. doi: 10.1111\/j.1574-6968.2009.01541.x","journal-title":"FEMS Microbiol Lett"},{"issue":"6","key":"5930_CR6","doi-asserted-by":"publisher","first-page":"347","DOI":"10.2165\/11595610-000000000-00000","volume":"25","author":"J Borysowski","year":"2011","unstructured":"Borysowski J, Lobocka M, Miedzybrodzki R, Weber-Dabrowska B, Gorski A (2011) Potential of bacteriophages and their lysins in the treatment of MRSA: current status and future perspectives. BioDrugs 25(6):347\u2013355. doi: 10.2165\/11595610-000000000-00000","journal-title":"BioDrugs"},{"key":"5930_CR7","volume-title":"Antibiotic resistance threats in the United States, 2013","author":"CDC","year":"2013","unstructured":"CDC (2013) Antibiotic resistance threats in the United States, 2013. Centers for Disease Control and Prevention, Atlanta"},{"issue":"1\u20132","key":"5930_CR8","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1016\/j.vetmic.2007.12.004","volume":"130","author":"LK Celia","year":"2008","unstructured":"Celia LK, Nelson D, Kerr DE (2008) Characterization of a bacteriophage lysin (Ply700) from Streptococcus uberis. Vet Microbiol 130(1\u20132):107\u2013117. doi: 10.1016\/j.vetmic.2007.12.004","journal-title":"Vet Microbiol"},{"issue":"3","key":"5930_CR9","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1111\/j.1432-1033.1993.tb18076.x","volume":"215","author":"M Danner","year":"1993","unstructured":"Danner M, Fuchs A, Miller S, Seckler R (1993) Folding and assembly of phage P22 tailspike endorhamnosidase lacking the N-terminal, head-binding domain. Eur J Biochem 215(3):653\u2013661","journal-title":"Eur J Biochem"},{"issue":"2","key":"5930_CR10","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1128\/IAI.00651-10","volume":"79","author":"KM Davis","year":"2011","unstructured":"Davis KM, Weiser JN (2011) Modifications to the peptidoglycan backbone help bacteria to establish infection. Infect Immun 79(2):562\u2013570. doi: 10.1128\/IAI.00651-10","journal-title":"Infect Immun"},{"issue":"1","key":"5930_CR11","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1111\/j.1574-6968.2006.00483.x","volume":"265","author":"DM Donovan","year":"2006","unstructured":"Donovan DM, Lardeo M, Foster-Frey J (2006) Lysis of staphylococcal mastitis pathogens by bacteriophage phi11 endolysin. FEMS Microbiol Lett 265(1):133\u2013139. doi: 10.1111\/j.1574-6968.2006.00483.x","journal-title":"FEMS Microbiol Lett"},{"issue":"12","key":"5930_CR12","doi-asserted-by":"publisher","first-page":"2023","DOI":"10.1110\/ps.035436.108","volume":"17","author":"OD Ekici","year":"2008","unstructured":"Ekici OD, Paetzel M, Dalbey RE (2008) Unconventional serine proteases: variations on the catalytic Ser\/His\/Asp triad configuration. Protein Sci Publ Protein Soc 17(12):2023\u20132037. doi: 10.1110\/ps.035436.108","journal-title":"Protein Sci Publ Protein Soc"},{"key":"5930_CR13","doi-asserted-by":"publisher","first-page":"1087","DOI":"10.1038\/ncomms2084","volume":"3","author":"JA Fallas","year":"2012","unstructured":"Fallas JA, Hartgerink JD (2012) Computational design of self-assembling register-specific collagen heterotrimers. Nat Commun 3:1087. doi: 10.1038\/ncomms2084","journal-title":"Nat Commun"},{"issue":"4","key":"5930_CR14","doi-asserted-by":"publisher","first-page":"1025","DOI":"10.1111\/j.1365-2672.2011.05119.x","volume":"111","author":"M Fenton","year":"2011","unstructured":"Fenton M, Ross RP, McAuliffe O, O\u2019Mahony J, Coffey A (2011) Characterization of the staphylococcal bacteriophage lysin CHAP(K). J Appl Microbiol 111(4):1025\u20131035. doi: 10.1111\/j.1365-2672.2011.05119.x","journal-title":"J Appl Microbiol"},{"issue":"5","key":"5930_CR15","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1016\/j.biochi.2010.01.026","volume":"92","author":"LY Filatova","year":"2010","unstructured":"Filatova LY, Becker SC, Donovan DM, Gladilin AK, Klyachko NL (2010) LysK, the enzyme lysing Staphylococcus aureus cells: specific kinetic features and approaches towards stabilization. Biochimie 92(5):507\u2013513. doi: 10.1016\/j.biochi.2010.01.026","journal-title":"Biochimie"},{"issue":"10","key":"5930_CR16","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1016\/j.tim.2005.08.007","volume":"13","author":"VA Fischetti","year":"2005","unstructured":"Fischetti VA (2005) Bacteriophage lytic enzymes: novel anti-infectives. Trends Microbiol 13(10):491\u2013496. doi: 10.1016\/j.tim.2005.08.007","journal-title":"Trends Microbiol"},{"issue":"3","key":"5930_CR17","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1016\/j.ijfoodmicro.2010.04.029","volume":"141","author":"P Garcia","year":"2010","unstructured":"Garcia P, Martinez B, Rodriguez L, Rodriguez A (2010) Synergy between the phage endolysin LysH5 and nisin to kill Staphylococcus aureus in pasteurized milk. Int J Food Microbiol 141(3):151\u2013155. doi: 10.1016\/j.ijfoodmicro.2010.04.029","journal-title":"Int J Food Microbiol"},{"issue":"6","key":"5930_CR18","doi-asserted-by":"publisher","first-page":"2743","DOI":"10.1128\/AAC.02526-12","volume":"57","author":"DB Gilmer","year":"2013","unstructured":"Gilmer DB, Schmitz JE, Euler CW, Fischetti VA (2013) Novel bacteriophage lysin with broad lytic activity protects against mixed infection by Streptococcus pyogenes and methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 57(6):2743\u20132750. doi: 10.1128\/AAC.02526-12","journal-title":"Antimicrob Agents Chemother"},{"issue":"5","key":"5930_CR19","doi-asserted-by":"publisher","first-page":"e1004109","DOI":"10.1371\/journal.ppat.1004109","volume":"10","author":"J Gu","year":"2014","unstructured":"Gu J, Feng Y, Feng X, Sun C, Lei L, Ding W, Niu F, Jiao L, Yang M, Li Y, Liu X, Song J, Cui Z, Han D, Du C, Yang Y, Ouyang S, Liu ZJ, Han W (2014) Structural and biochemical characterization reveals LysGH15 as an unprecedented \u201cEF-hand-like\u201d calcium-binding phage lysin. PLoS Pathog 10(5):e1004109. doi: 10.1371\/journal.ppat.1004109","journal-title":"PLoS Pathog"},{"issue":"1","key":"5930_CR20","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1128\/CMR.00042-09","volume":"23","author":"BP Howden","year":"2010","unstructured":"Howden BP, Davies JK, Johnson PD, Stinear TP, Grayson ML (2010) Reduced vancomycin susceptibility in Staphylococcus aureus, including vancomycin-intermediate and heterogeneous vancomycin-intermediate strains: resistance mechanisms, laboratory detection, and clinical implications. Clin Microbiol Rev 23(1):99\u2013139. doi: 10.1128\/CMR.00042-09","journal-title":"Clin Microbiol Rev"},{"issue":"24","key":"5930_CR21","doi-asserted-by":"publisher","first-page":"2255","DOI":"10.1056\/NEJMoa1207290","volume":"368","author":"SS Huang","year":"2013","unstructured":"Huang SS, Septimus E, Kleinman K, Moody J, Hickok J, Avery TR, Lankiewicz J, Gombosev A, Terpstra L, Hartford F, Hayden MK, Jernigan JA, Weinstein RA, Fraser VJ, Haffenreffer K, Cui E, Kaganov RE, Lolans K, Perlin JB, Platt R, Program CDCPE, Network AD, Healthcare-Associated Infections P (2013) Targeted versus universal decolonization to prevent ICU infection. N Engl J Med 368(24):2255\u20132265. doi: 10.1056\/NEJMoa1207290","journal-title":"N Engl J Med"},{"key":"5930_CR22","first-page":"972","volume-title":"Manual of clinical microbiology","author":"R Jones","year":"1985","unstructured":"Jones R, Barry AL, Gavan T et al (1985) Susceptibility tests: microdilution and macrodilution broth procedures. In: Balows A, Hausler J, Shadomy H (eds) Manual of clinical microbiology. American Society for Microbiology, Washington, DC, pp 972\u2013977"},{"issue":"4","key":"5930_CR23","doi-asserted-by":"publisher","first-page":"1764","DOI":"10.1128\/AAC.01097-10","volume":"55","author":"SY Jun","year":"2011","unstructured":"Jun SY, Jung GM, Son JS, Yoon SJ, Choi YJ, Kang SH (2011) Comparison of the antibacterial properties of phage endolysins SAL-1 and LysK. Antimicrob Agents Chemother 55(4):1764\u20131767. doi: 10.1128\/AAC.01097-10","journal-title":"Antimicrob Agents Chemother"},{"issue":"21","key":"5930_CR24","doi-asserted-by":"crossref","first-page":"6344","DOI":"10.1021\/bi010158b","volume":"40","author":"R Khayat","year":"2001","unstructured":"Khayat R, Batra R, Massariol MJ, Lagace L, Tong L (2001) Investigating the role of histidine 157 in the catalytic activity of human cytomegalovirus protease. Biochemistry 40(21):6344\u20136351","journal-title":"Biochemistry"},{"issue":"8","key":"5930_CR25","doi-asserted-by":"publisher","first-page":"3256","DOI":"10.1128\/AAC.49.8.3256-3263.2005","volume":"49","author":"CM Kusuma","year":"2005","unstructured":"Kusuma CM, Kokai-Kun JF (2005) Comparison of four methods for determining lysostaphin susceptibility of various strains of Staphylococcus aureus. Antimicrob Agents Chemother 49(8):3256\u20133263. doi: 10.1128\/AAC.49.8.3256-3263.2005","journal-title":"Antimicrob Agents Chemother"},{"issue":"1","key":"5930_CR26","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1128\/AAC.47.1.375-377.2003","volume":"47","author":"JM Loeffler","year":"2003","unstructured":"Loeffler JM, Fischetti VA (2003) Synergistic lethal effect of a combination of phage lytic enzymes with different activities on penicillin-sensitive and -resistant Streptococcus pneumoniae strains. Antimicrob Agents Chemother 47(1):375\u2013377","journal-title":"Antimicrob Agents Chemother"},{"issue":"39","key":"5930_CR27","doi-asserted-by":"publisher","first-page":"34391","DOI":"10.1074\/jbc.M111.244160","volume":"286","author":"LY Low","year":"2011","unstructured":"Low LY, Yang C, Perego M, Osterman A, Liddington R (2011) Role of net charge on catalytic domain and influence of cell wall binding domain on bactericidal activity, specificity, and host range of phage lysins. J Biol Chem 286(39):34391\u201334403. doi: 10.1074\/jbc.M111.244160","journal-title":"J Biol Chem"},{"issue":"8","key":"5930_CR28","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1056\/NEJM199808203390806","volume":"339","author":"FD Lowy","year":"1998","unstructured":"Lowy FD (1998) Staphylococcus aureus infections. N Engl J Med 339(8):520\u2013532. doi: 10.1056\/NEJM199808203390806","journal-title":"N Engl J Med"},{"issue":"31","key":"5930_CR29","doi-asserted-by":"publisher","first-page":"12752","DOI":"10.1073\/pnas.1208424109","volume":"109","author":"S McGowan","year":"2012","unstructured":"McGowan S, Buckle AM, Mitchell MS, Hoopes JT, Gallagher DT, Heselpoth RD, Shen Y, Reboul CF, Law RH, Fischetti VA, Whisstock JC, Nelson DC (2012) X-ray crystal structure of the streptococcal specific phage lysin PlyC. Proc Natl Acad Sci U S A 109(31):12752\u201312757. doi: 10.1073\/pnas.1208424109","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"Suppl 3","key":"5930_CR30","doi-asserted-by":"publisher","first-page":"S184","DOI":"10.1086\/519471","volume":"45","author":"ST Micek","year":"2007","unstructured":"Micek ST (2007) Alternatives to vancomycin for the treatment of methicillin-resistant Staphylococcus aureus infections. Clin Infect Dis Off Publ Infect Dis Soc Am 45(Suppl 3):S184\u2013S190. doi: 10.1086\/519471","journal-title":"Clin Infect Dis Off Publ Infect Dis Soc Am"},{"key":"5930_CR31","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/0003-2697(67)90057-7","volume":"18","author":"L Mokrasch","year":"1967","unstructured":"Mokrasch L (1967) Use of 2,4,6-trinitrobenzenesulfonic acid for the coestimation of amines, amino acids, and proteins in mixtures. Anal Biochem 18:64\u201371","journal-title":"Anal Biochem"},{"key":"5930_CR32","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1016\/B978-0-12-394438-2.00007-4","volume":"83","author":"DC Nelson","year":"2012","unstructured":"Nelson DC, Schmelcher M, Rodriguez-Rubio L, Klumpp J, Pritchard DG, Dong S, Donovan DM (2012) Endolysins as antimicrobials. Adv Virus Res 83:299\u2013365. doi: 10.1016\/B978-0-12-394438-2.00007-4","journal-title":"Adv Virus Res"},{"issue":"Pt 7","key":"5930_CR33","doi-asserted-by":"publisher","first-page":"2079","DOI":"10.1099\/mic.0.27063-0","volume":"150","author":"DG Pritchard","year":"2004","unstructured":"Pritchard DG, Dong S, Baker JR, Engler JA (2004) The bifunctional peptidoglycan lysin of Streptococcus agalactiae bacteriophage B30. Microbiology 150(Pt 7):2079\u20132087. doi: 10.1099\/mic.0.27063-0","journal-title":"Microbiology"},{"issue":"1","key":"5930_CR34","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1021\/bi00504a006","volume":"20","author":"SW Provencher","year":"1981","unstructured":"Provencher SW, Glockner J (1981) Estimation of globular protein secondary structure from circular dichroism. Biochemistry 20(1):33\u201337","journal-title":"Biochemistry"},{"issue":"5","key":"5930_CR35","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/S0968-0004(03)00062-8","volume":"28","author":"DJ Rigden","year":"2003","unstructured":"Rigden DJ, Jedrzejas MJ, Galperin MY (2003) Amidase domains from bacterial and phage autolysins define a family of gamma-D, L-glutamate-specific amidohydrolases. Trends Biochem Sci 28(5):230\u2013234","journal-title":"Trends Biochem Sci"},{"issue":"9","key":"5930_CR36","doi-asserted-by":"crossref","first-page":"6125","DOI":"10.1016\/S0021-9258(18)53228-2","volume":"268","author":"JM Sanz","year":"1993","unstructured":"Sanz JM, Garcia JL, Laynez J, Usobiaga P, Menendez M (1993) Thermal stability and cooperative domains of CPL1 lysozyme and its NH2- and COOH-terminal modules. Dependence on choline binding. J Biol Chem 268(9):6125\u20136130","journal-title":"J Biol Chem"},{"issue":"1","key":"5930_CR37","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1128\/AEM.01616-06","volume":"73","author":"P Sass","year":"2007","unstructured":"Sass P, Bierbaum G (2007) Lytic activity of recombinant bacteriophage phi11 and phi12 endolysins on whole cells and biofilms of Staphylococcus aureus. Appl Environ Microbiol 73(1):347\u2013352. doi: 10.1128\/AEM.01616-06","journal-title":"Appl Environ Microbiol"},{"key":"5930_CR38","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/0006-3002(60)90028-7","volume":"41","author":"M Satake","year":"1960","unstructured":"Satake M, Mase K, Takahashi Y, Ogata K (1960) Incorporation of leucine into microsomal protein by a cell-free system of guinea-pig brain. Biochim Biophys Acta 41:366\u2013367","journal-title":"Biochim Biophys Acta"},{"issue":"10","key":"5930_CR39","doi-asserted-by":"publisher","first-page":"1147","DOI":"10.2217\/fmb.12.97","volume":"7","author":"M Schmelcher","year":"2012","unstructured":"Schmelcher M, Donovan DM, Loessner MJ (2012a) Bacteriophage endolysins as novel antimicrobials. Future Microbiol 7(10):1147\u20131171. doi: 10.2217\/fmb.12.97","journal-title":"Future Microbiol"},{"issue":"7","key":"5930_CR40","doi-asserted-by":"publisher","first-page":"2297","DOI":"10.1128\/AEM.07050-11","volume":"78","author":"M Schmelcher","year":"2012","unstructured":"Schmelcher M, Powell AM, Becker SC, Camp MJ, Donovan DM (2012b) Chimeric phage lysins act synergistically with lysostaphin to kill mastitis-causing Staphylococcus aureus in murine mammary glands. Appl Environ Microbiol 78(7):2297\u20132305. doi: 10.1128\/AEM.07050-11","journal-title":"Appl Environ Microbiol"},{"issue":"2","key":"5930_CR41","doi-asserted-by":"publisher","first-page":"633","DOI":"10.1007\/s00253-011-3372-6","volume":"93","author":"M Schmelcher","year":"2012","unstructured":"Schmelcher M, Waldherr F, Loessner MJ (2012c) Listeria bacteriophage peptidoglycan hydrolases feature high thermoresistance and reveal increased activity after divalent metal cation substitution. Appl Microbiol Biotechnol 93(2):633\u2013643. doi: 10.1007\/s00253-011-3372-6","journal-title":"Appl Microbiol Biotechnol"},{"issue":"6900","key":"5930_CR42","doi-asserted-by":"publisher","first-page":"884","DOI":"10.1038\/nature01026","volume":"418","author":"R Schuch","year":"2002","unstructured":"Schuch R, Nelson D, Fischetti VA (2002) A bacteriolytic agent that detects and kills Bacillus anthracis. Nature 418(6900):884\u2013889. doi: 10.1038\/nature01026","journal-title":"Nature"},{"key":"5930_CR43","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit637","author":"R Schuch","year":"2013","unstructured":"Schuch R, Lee HM, Schneider BC, Sauve KL, Law C, Khan BK, Rotolo JA, Horiuchi Y, Couto DE, Raz A, Fischetti VA, Huang DB, Nowinski RC, Wittekind M (2013) Combination therapy with lysin CF-301 and antibiotic is superior to antibiotic alone for treating methicillin-resistant Staphylococcus aureus-induced murine bacteremia. J Infect Dis. doi: 10.1093\/infdis\/jit637","journal-title":"J Infect Dis"},{"key":"5930_CR44","doi-asserted-by":"crossref","unstructured":"Shen Y, Mitchell M, Donovan DM, Nelson DC (2012) Phage-based enzybiotics. In: Abedon S, Hyman P (eds) Bacteriophages in health and disease. CABI Press, pp 217\u2013239","DOI":"10.1079\/9781845939847.0217"},{"issue":"24","key":"5930_CR45","doi-asserted-by":"crossref","first-page":"7103","DOI":"10.1128\/JB.185.24.7103-7110.2003","volume":"185","author":"K Sieradzki","year":"2003","unstructured":"Sieradzki K, Tomasz A (2003) Alterations of cell wall structure and metabolism accompany reduced susceptibility to vancomycin in an isogenic series of clinical isolates of Staphylococcus aureus. J Bacteriol 185(24):7103\u20137110","journal-title":"J Bacteriol"},{"key":"5930_CR46","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1186\/1471-2180-12-33","volume":"12","author":"B Son","year":"2012","unstructured":"Son B, Yun J, Lim JA, Shin H, Heu S, Ryu S (2012) Characterization of LysB4, an endolysin from the Bacillus cereus-infecting bacteriophage B4. BMC Microbiol 12:33. doi: 10.1186\/1471-2180-12-33","journal-title":"BMC Microbiol"},{"issue":"5157","key":"5930_CR47","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1126\/science.8153626","volume":"264","author":"BG Spratt","year":"1994","unstructured":"Spratt BG (1994) Resistance to antibiotics mediated by target alterations. Science 264(5157):388\u2013393","journal-title":"Science"},{"issue":"10","key":"5930_CR48","doi-asserted-by":"publisher","first-page":"854","DOI":"10.1016\/j.resmic.2010.09.011","volume":"161","author":"R Sunagar","year":"2010","unstructured":"Sunagar R, Patil SA, Chandrakanth RK (2010) Bacteriophage therapy for Staphylococcus aureus bacteremia in streptozotocin-induced diabetic mice. Res Microbiol 161(10):854\u2013860. doi: 10.1016\/j.resmic.2010.09.011","journal-title":"Res Microbiol"},{"key":"5930_CR49","doi-asserted-by":"publisher","unstructured":"Swift SM, Nelson DC (2014) Complete genome sequence of Staphylococcus aureus phage GRCS. Genome Announc 2(2) doi: 10.1128\/genomeA.00209-14","DOI":"10.1128\/genomeA.00209-14"},{"issue":"1","key":"5930_CR50","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/j.cll.2010.01.005","volume":"30","author":"YW Tang","year":"2010","unstructured":"Tang YW, Stratton CW (2010) Staphylococcus aureus: an old pathogen with new weapons. Clin Lab Med 30(1):179\u2013208. doi: 10.1016\/j.cll.2010.01.005","journal-title":"Clin Lab Med"},{"issue":"5887","key":"5930_CR51","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1126\/science.321.5887.356","volume":"321","author":"G Taubes","year":"2008","unstructured":"Taubes G (2008) The bacteria fight back. Science 321(5887):356\u2013361. doi: 10.1126\/science.321.5887.356","journal-title":"Science"},{"issue":"2","key":"5930_CR52","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1111\/j.1574-6976.2007.00088.x","volume":"32","author":"W Vollmer","year":"2008","unstructured":"Vollmer W (2008) Structural variation in the glycan strands of bacterial peptidoglycan. FEMS Microbiol Rev 32(2):287\u2013306. doi: 10.1111\/j.1574-6976.2007.00088.x","journal-title":"FEMS Microbiol Rev"},{"issue":"3","key":"5930_CR53","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1038\/nm991","volume":"10","author":"C Weidenmaier","year":"2004","unstructured":"Weidenmaier C, Kokai-Kun JF, Kristian SA, Chanturiya T, Kalbacher H, Gross M, Nicholson G, Neumeister B, Mond JJ, Peschel A (2004) Role of teichoic acids in Staphylococcus aureus nasal colonization, a major risk factor in nosocomial infections. Nat Med 10(3):243\u2013245. doi: 10.1038\/nm991","journal-title":"Nat Med"},{"key":"5930_CR54","doi-asserted-by":"publisher","unstructured":"Whitmore L, Wallace BA (2004) DICHROWEB, an online server for protein secondary structure analyses from circular dichroism spectroscopic data. Nucleic Acids Res 32(Web Server issue):W668-73 doi: 10.1093\/nar\/gkh371","DOI":"10.1093\/nar\/gkh371"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-014-5930-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00253-014-5930-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-014-5930-1","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-014-5930-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,11]],"date-time":"2022-04-11T13:50:00Z","timestamp":1649685000000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00253-014-5930-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7,20]]},"references-count":54,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,1]]}},"alternative-id":["5930"],"URL":"https:\/\/doi.org\/10.1007\/s00253-014-5930-1","relation":{},"ISSN":["0175-7598","1432-0614"],"issn-type":[{"value":"0175-7598","type":"print"},{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,20]]}}}