{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T03:13:21Z","timestamp":1788923601470,"version":"build-2803163510"},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"20","license":[{"start":{"date-parts":[[2017,9,14]],"date-time":"2017-09-14T00:00:00Z","timestamp":1505347200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"TU Wien (TUW)"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"published-print":{"date-parts":[[2017,10]]},"DOI":"10.1007\/s00253-017-8475-2","type":"journal-article","created":{"date-parts":[[2017,9,14]],"date-time":"2017-09-14T10:19:33Z","timestamp":1505384373000},"page":"7675-7688","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Morphological analysis of the filamentous fungus Penicillium chrysogenum using flow cytometry\u2014the fast alternative to microscopic image analysis"],"prefix":"10.1007","volume":"101","author":[{"given":"Daniela","family":"Ehgartner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Herwig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Fricke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,9,14]]},"reference":[{"key":"8475_CR1","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1002\/bit.260080411","volume":"8","author":"EB Chain","year":"1966","unstructured":"Chain EB, Gualandi G, Morisi G (1966) Aeration studies. IV. Aeration conditions in 3000-liter submerged fermentations with various microorganisms. Biotechnol Bioeng 8:595\u2013619. \nhttps:\/\/doi.org\/10.1002\/bit.260080411","journal-title":"Biotechnol Bioeng"},{"key":"8475_CR2","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1002\/(SICI)1097-0290(19990420)63:2<147::AID-BIT3>3.0.CO;2-M","volume":"63","author":"T Christiansen","year":"1999","unstructured":"Christiansen T, Spohr AB, Nielsen J (1999) On-line study of growth kinetics of single hyphae of Aspergillus oryzae in a flow-through cell. Biotechnol Bioeng 63:147\u2013153","journal-title":"Biotechnol Bioeng"},{"issue":"Pt 4","key":"8475_CR3","doi-asserted-by":"publisher","first-page":"817","DOI":"10.1099\/00221287-144-4-817","volume":"144","author":"PW Cox","year":"1998","unstructured":"Cox PW, Paul GC, Thomas CR (1998) Image analysis of the morphology of filamentous micro-organisms. Microbiol 144(Pt 4):817\u2013827. \nhttps:\/\/doi.org\/10.1099\/00221287-144-4-817","journal-title":"Microbiol"},{"key":"8475_CR4","doi-asserted-by":"publisher","first-page":"1263","DOI":"10.1128\/AEM.02134-10","volume":"77","author":"C Bekker de","year":"2011","unstructured":"de Bekker C, van Veluw GJ, Vinck A, Wiebenga LA, W\u00f6sten HA (2011) Heterogeneity of Aspergillus niger microcolonies in liquid shaken cultures. Appl Environ Microbiol 77:1263\u20131267. \nhttps:\/\/doi.org\/10.1128\/AEM.02134-10","journal-title":"Appl Environ Microbiol"},{"key":"8475_CR5","doi-asserted-by":"publisher","first-page":"2271","DOI":"10.1534\/g3.114.014357","volume":"4","author":"L Delgado-Ramos","year":"2014","unstructured":"Delgado-Ramos L, Marcos AT, Ramos-Guelfo MS, Sanchez-Barrionuevo L, Smet F, Chavez S, Canovas D (2014) Flow cytometry of microencapsulated colonies for genetics analysis of filamentous fungi. G3: Genes genomes gen 4:2271\u20132278. \nhttps:\/\/doi.org\/10.1534\/g3.114.014357","journal-title":"G3: Genes genomes gen"},{"key":"8475_CR6","doi-asserted-by":"publisher","first-page":"255","DOI":"10.3989\/scimar.2000.64n2255","volume":"64","author":"GBJ Dubelaar","year":"2000","unstructured":"Dubelaar GBJ, Gerritzen PL (2000) CytoBuoy: a step forward towards using flow cytometry in operational oceanography. Sci Mar 64:255\u2013265. \nhttps:\/\/doi.org\/10.3989\/scimar.2000.64n2255","journal-title":"Sci Mar"},{"key":"8475_CR7","doi-asserted-by":"publisher","first-page":"946","DOI":"10.1039\/b409350j","volume":"6","author":"GB Dubelaar","year":"2004","unstructured":"Dubelaar GB, Geerders PJ, Jonker RR (2004) High frequency monitoring reveals phytoplankton dynamics. J Environ Monit 6:946\u2013952. \nhttps:\/\/doi.org\/10.1039\/b409350j","journal-title":"J Environ Monit"},{"key":"8475_CR8","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1002\/jctb.4469","volume":"90","author":"D Ehgartner","year":"2015","unstructured":"Ehgartner D, Sagmeister P, Herwig C, Wechselberger P (2015) A novel real-time method to estimate volumetric mass biodensity based on the combination of dielectric spectroscopy and soft-sensors. J Chem Technol Biotechnol 90:262\u2013272","journal-title":"J Chem Technol Biotechnol"},{"key":"8475_CR9","doi-asserted-by":"publisher","first-page":"8923","DOI":"10.1007\/s00253-016-7787-y","volume":"100","author":"D Ehgartner","year":"2016","unstructured":"Ehgartner D, Fricke J, Schroder A, Herwig C (2016a) At-line determining spore germination of Penicillium chrysogenum bioprocesses in complex media. Appl Microbiol Biotechnol 100:8923\u20138930. \nhttps:\/\/doi.org\/10.1007\/s00253-016-7787-y","journal-title":"Appl Microbiol Biotechnol"},{"key":"8475_CR10","doi-asserted-by":"publisher","first-page":"5363","DOI":"10.1007\/s00253-016-7319-9","volume":"100","author":"D Ehgartner","year":"2016","unstructured":"Ehgartner D, Herwig C, Neutsch L (2016b) At-line determination of spore inoculum quality in Penicillium chrysogenum bioprocesses. Appl Microbiol Biotechnol 100:5363\u20135373. \nhttps:\/\/doi.org\/10.1007\/s00253-016-7319-9","journal-title":"Appl Microbiol Biotechnol"},{"key":"8475_CR11","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1080\/07388550091144177","volume":"20","author":"PA Gibbs","year":"2000","unstructured":"Gibbs PA, Seviour RJ, Schmid F (2000) Growth of filamentous fungi in submerged culture: problems and possible solutions. Crit Rev Biotechnol 20:17\u201348. \nhttps:\/\/doi.org\/10.1080\/07388550091144177","journal-title":"Crit Rev Biotechnol"},{"key":"8475_CR12","series-title":"Fungal biology","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1007\/978-3-319-10503-1_17","volume-title":"Genetic transformation systems in fungi","author":"A Golabgir","year":"2015","unstructured":"Golabgir A, Ehgartner D, Neutsch L, Posch AE, Sagmeister P, Herwig C (2015) Imaging flow cytometry and high-throughput microscopy for automated macroscopic morphological analysis of filamentous fungi. In: van den Berg MA, Maruthachalam K (eds) Genetic transformation systems in fungi, Fungal biology, vol 2. Springer International Publishing, Cham, pp 201\u2013210. \nhttps:\/\/doi.org\/10.1007\/978-3-319-10503-1_17"},{"key":"8475_CR13","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1007\/s00253-015-7089-9","volume":"100","author":"T Langemann","year":"2016","unstructured":"Langemann T, Mayr UB, Meitz A, Lubitz W, Herwig C (2016) Multi-parameter flow cytometry as a process analytical technology (PAT) approach for the assessment of bacterial ghost production. Appl Microbiol Biotechnol 100:409\u2013418. \nhttps:\/\/doi.org\/10.1007\/s00253-015-7089-9","journal-title":"Appl Microbiol Biotechnol"},{"key":"8475_CR14","first-page":"105","volume-title":"Overproduction of microbial metabolites","author":"J Lein","year":"1986","unstructured":"Lein J (1986) The penicillin strain improvement program. In: Vanek Z, Hostalek Z (eds) Overproduction of microbial metabolites. Butterworths, Boston, pp 105\u2013139"},{"key":"8475_CR15","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.biotechadv.2003.09.005","volume":"22","author":"M Papagianni","year":"2004","unstructured":"Papagianni M (2004) Fungal morphology and metabolite production in submerged mycelial processes. Biotechnol Adv 22:189\u2013259","journal-title":"Biotechnol Adv"},{"key":"8475_CR16","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/BFb0102278","volume":"60","author":"GC Paul","year":"1998","unstructured":"Paul GC, Thomas CR (1998) Characterisation of mycelial morphology using image analysis. Adv Biochem Eng Biotechnol 60:1\u201359. \nhttps:\/\/doi.org\/10.1007\/BFb0102278","journal-title":"Adv Biochem Eng Biotechnol"},{"key":"8475_CR17","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1002\/bit.260440513","volume":"44","author":"GC Paul","year":"1994","unstructured":"Paul GC, Kent CA, Thomas CR (1994) Hyphal vocuolation and fragmentation in penicillium chrysogenum. Biotechnol Bioeng 44:655\u2013660. \nhttps:\/\/doi.org\/10.1002\/bit.260440513","journal-title":"Biotechnol Bioeng"},{"key":"8475_CR18","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/S1369-703X(98)00012-6","volume":"2","author":"GC Paul","year":"1998","unstructured":"Paul GC, Syddall MT, Thomas CR (1998) A structured model for penicillin production on mixed substrates. Biochem Eng J 2:11. \nhttps:\/\/doi.org\/10.1016\/S1369-703X(98)00012-6","journal-title":"Biochem Eng J"},{"key":"8475_CR19","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1002\/bit.21732","volume":"100","author":"N Petersen","year":"2008","unstructured":"Petersen N, Stocks S, Gernaey KV (2008) Multivariate models for prediction of rheological characteristics of filamentous fermentation broth from the size distribution. Biotechnol Bioeng 100:61\u201371. \nhttps:\/\/doi.org\/10.1002\/bit.21732","journal-title":"Biotechnol Bioeng"},{"key":"8475_CR20","doi-asserted-by":"publisher","first-page":"689","DOI":"10.1002\/Btpr.1901","volume":"30","author":"AE Posch","year":"2014","unstructured":"Posch AE, Herwig C (2014) Physiological description of multivariate interdependencies between process parameters, morphology and physiology during fed-batch penicillin production. Biotechnol Prog 30:689\u2013699. \nhttps:\/\/doi.org\/10.1002\/Btpr.1901","journal-title":"Biotechnol Prog"},{"key":"8475_CR21","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1016\/j.fgb.2012.05.003","volume":"49","author":"AE Posch","year":"2012","unstructured":"Posch AE, Spadiut O, Herwig C (2012) A novel method for fast and statistically verified morphological characterization of filamentous fungi. Fungal Genet Biol 49:499\u2013510. \nhttps:\/\/doi.org\/10.1016\/j.fgb.2012.05.003","journal-title":"Fungal Genet Biol"},{"key":"8475_CR22","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1016\/j.mimet.2004.07.016","volume":"59","author":"V Prigione","year":"2004","unstructured":"Prigione V, Filipello Marchisio V (2004) Methods to maximise the staining of fungal propagules with fluorescent dyes. J Microbiol Methods 59:371\u2013379. \nhttps:\/\/doi.org\/10.1016\/j.mimet.2004.07.016","journal-title":"J Microbiol Methods"},{"key":"8475_CR23","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1007\/10_2015_309","volume":"149","author":"D Quintanilla","year":"2015","unstructured":"Quintanilla D, Hagemann T, Hansen K, Gernaey KV (2015) Fungal morphology in industrial enzyme production--modelling and monitoring. Adv Biochem Eng Biotechnol 149:29\u201354. \nhttps:\/\/doi.org\/10.1007\/10_2015_309","journal-title":"Adv Biochem Eng Biotechnol"},{"key":"8475_CR24","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1002\/(SICI)1097-0290(20000420)68:2<160::AID-BIT5>3.0.CO;2-P","volume":"68","author":"GL Riley","year":"2000","unstructured":"Riley GL, Tucker KG, Paul GC, Thomas CR (2000) Effect of biomass concentration and mycelial morphology on fermentation broth rheology. Biotechnol Bioeng 68:160\u2013172. \nhttps:\/\/doi.org\/10.1002\/(SICI)1097-0290(20000420)68:2<160::AID-BIT5>3.0.CO;2-P","journal-title":"Biotechnol Bioeng"},{"key":"8475_CR25","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1002\/(SICI)1097-0290(19980605)58:5<541::AID-BIT11>3.0.CO;2-E","volume":"58","author":"A Spohr","year":"1998","unstructured":"Spohr A, Dam-Mikkelsen C, Carlsen M, Nielsen J, Villadsen J (1998) On-line study of fungal morphology during submerged growth in a small flow-through cell. Biotechnol Bioeng 58:541\u2013553. \nhttps:\/\/doi.org\/10.1002\/(SICI)1097-0290(19980605)58:5<541::AID-BIT11>3.0.CO;2-E","journal-title":"Biotechnol Bioeng"},{"key":"8475_CR26","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1002\/bit.260480103","volume":"48","author":"B Vanhoutte","year":"1995","unstructured":"Vanhoutte B, Pons MN, Thomas CR, Louvel L, Vivier H (1995) Characterization of Penicillium chrysogenum physiology in submerged cultures by color and monochrome image analysis. Biotechnol Bioeng 48:1\u201311. \nhttps:\/\/doi.org\/10.1002\/bit.260480103","journal-title":"Biotechnol Bioeng"},{"key":"8475_CR27","first-page":"237","volume":"39","author":"P Znidarsic","year":"2001","unstructured":"Znidarsic P, Pavko A (2001) The morphology of filamentous fungi in submerged cultivations as a bioprocess parameter. Food Technol Biotechnol 39:237\u2013252","journal-title":"Food Technol Biotechnol"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00253-017-8475-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-017-8475-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-017-8475-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,10,2]],"date-time":"2017-10-02T03:58:51Z","timestamp":1506916731000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00253-017-8475-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,14]]},"references-count":27,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2017,10]]}},"alternative-id":["8475"],"URL":"https:\/\/doi.org\/10.1007\/s00253-017-8475-2","relation":{},"ISSN":["0175-7598","1432-0614"],"issn-type":[{"value":"0175-7598","type":"print"},{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,14]]}}}