{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T04:22:45Z","timestamp":1791951765627,"version":"4.3.4"},"reference-count":52,"publisher":"Springer Science and Business Media LLC","issue":"21","license":[{"start":{"date-parts":[[2018,8,29]],"date-time":"2018-08-29T00:00:00Z","timestamp":1535500800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100004366","name":"Tabriz University of Medical Sciences","doi-asserted-by":"publisher","award":["RCPN5960"],"award-info":[{"award-number":["RCPN5960"]}],"id":[{"id":"10.13039\/501100004366","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"published-print":{"date-parts":[[2018,11]]},"DOI":"10.1007\/s00253-018-9296-7","type":"journal-article","created":{"date-parts":[[2018,8,30]],"date-time":"2018-08-30T00:54:56Z","timestamp":1535590496000},"page":"9267-9278","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Stable transformation of Spirulina (Arthrospira) platensis: a promising microalga for production of edible vaccines"],"prefix":"10.1007","volume":"102","author":[{"given":"Jaber","family":"Dehghani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khosro","family":"Adibkia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Movafeghi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abolfazl","family":"Barzegari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad M.","family":"Pourseif","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hadi","family":"Maleki Kakelar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asal","family":"Golchin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0067-2475","authenticated-orcid":false,"given":"Yadollah","family":"Omidi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,8,29]]},"reference":[{"issue":"24","key":"9296_CR1","doi-asserted-by":"publisher","first-page":"25359","DOI":"10.1074\/jbc.M401804200","volume":"279","author":"A Abu-Arish","year":"2004","unstructured":"Abu-Arish A, Frenkiel-Krispin D, Fricke T, Tzfira T, Citovsky V, Wolf SG, Elbaum M (2004) Three-dimensional reconstruction of Agrobacterium VirE2 protein with single-stranded DNA. J Biol Chem 279(24):25359\u201325363. \n                    https:\/\/doi.org\/10.1074\/jbc.M401804200","journal-title":"J Biol Chem"},{"issue":"1","key":"9296_CR2","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1080\/09670262.2010.550386","volume":"46","author":"N Anila","year":"2011","unstructured":"Anila N, Chandrashekar A, Ravishankar G, Sarada R (2011) Establishment of Agrobacterium tumefaciens-mediated genetic transformation in Dunaliella bardawil. Eur J Phycol 46(1):36\u201344. \n                    https:\/\/doi.org\/10.1080\/09670262.2010.550386","journal-title":"Eur J Phycol"},{"issue":"4","key":"9296_CR3","first-page":"562","volume":"79","author":"S Atashpaz","year":"2010","unstructured":"Atashpaz S, Khani S, Barzegari A, Barar J, Vahed SZ, Azarbaijani R, Omidi Y (2010) A robust universal method for extraction of genomic DNA from bacterial species. Mikrobiologiia 79(4):562\u2013566","journal-title":"Mikrobiologiia"},{"issue":"8","key":"9296_CR4","doi-asserted-by":"publisher","first-page":"2497","DOI":"10.4161\/hv.29032","volume":"10","author":"A Barzegari","year":"2014","unstructured":"Barzegari A, Saeedi N, Zarredar H, Barar J, Omidi Y (2014) The search for a promising cell factory system for production of edible vaccine. Hum Vaccin Immunother 10(8):2497\u20132502. \n                    https:\/\/doi.org\/10.4161\/hv.29032","journal-title":"Hum Vaccin Immunother"},{"issue":"4","key":"9296_CR5","doi-asserted-by":"publisher","first-page":"1771","DOI":"10.1007\/s11274-011-0991-0","volume":"28","author":"TS Cha","year":"2012","unstructured":"Cha TS, Yee W, Aziz A (2012) Assessment of factors affecting Agrobacterium-mediated genetic transformation of the unicellular green alga, Chlorella vulgaris. World J Microbiol Biotechnol 28(4):1771\u20131779. \n                    https:\/\/doi.org\/10.1007\/s11274-011-0991-0","journal-title":"World J Microbiol Biotechnol"},{"key":"9296_CR6","doi-asserted-by":"publisher","first-page":"24253","DOI":"10.1038\/srep24253","volume":"6","author":"YH Chen","year":"2016","unstructured":"Chen YH, Chang GK, Kuo SM, Huang SY, Hu IC, Lo YL, Shih SR (2016) Well-tolerated Spirulina extract inhibits influenza virus replication and reduces virus-induced mortality. Sci Rep 6:24253. \n                    https:\/\/doi.org\/10.1038\/srep24253","journal-title":"Sci Rep"},{"issue":"3","key":"9296_CR7","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/j.micres.2011.05.003","volume":"167","author":"R Cheng","year":"2012","unstructured":"Cheng R, Ma R, Li K, Rong H, Lin X, Wang Z, Yang S, Ma Y (2012) Agrobacterium tumefaciens mediated transformation of marine microalgae Schizochytrium. Microbiol Res 167(3):179\u2013186. \n                    https:\/\/doi.org\/10.1016\/j.micres.2011.05.003","journal-title":"Microbiol Res"},{"issue":"9","key":"9296_CR8","doi-asserted-by":"publisher","first-page":"839","DOI":"10.1038\/nbt0998-839","volume":"16","author":"MJ Groot de","year":"1998","unstructured":"de Groot MJ, Bundock P, Hooykaas PJ, Beijersbergen AG (1998) Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Nat Biotechnol 16(9):839\u2013842. \n                    https:\/\/doi.org\/10.1038\/nbt0998-839","journal-title":"Nat Biotechnol"},{"issue":"4","key":"9296_CR9","doi-asserted-by":"publisher","first-page":"247","DOI":"10.15171\/bi.2017.29","volume":"7","author":"J Dehghani","year":"2017","unstructured":"Dehghani J, Movafeghi A, Barzegari A, Barar J (2017) Efficient and stable transformation of Dunaliella pseudosalina by 3 strains of Agrobacterium tumefaciens. Bioimpacts 7(4):247\u2013254. \n                    https:\/\/doi.org\/10.15171\/bi.2017.29","journal-title":"Bioimpacts"},{"issue":"1235","key":"9296_CR10","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1098\/rstb.1990.0006","volume":"326","author":"ES Dennis","year":"1990","unstructured":"Dennis ES, Brettell RI (1990) DNA methylation of maize transposable elements is correlated with activity. Philos Trans R Soc Lond Ser B Biol Sci 326(1235):217\u2013229","journal-title":"Philos Trans R Soc Lond Ser B Biol Sci"},{"key":"9296_CR11","doi-asserted-by":"publisher","first-page":"505","DOI":"10.3389\/fpls.2016.00505","volume":"7","author":"L Doron","year":"2016","unstructured":"Doron L, Segal N, Shapira M (2016) Transgene expression in microalgae-from tools to applications. Front Plant Sci 7:505. \n                    https:\/\/doi.org\/10.3389\/fpls.2016.00505","journal-title":"Front Plant Sci"},{"issue":"3","key":"9296_CR12","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1016\/j.jbiotec.2009.12.006","volume":"145","author":"IA Dreesen","year":"2010","unstructured":"Dreesen IA, Charpin-El Hamri G, Fussenegger M (2010) Heat-stable oral alga-based vaccine protects mice from Staphylococcus aureus infection. J Biotechnol 145(3):273\u2013280. \n                    https:\/\/doi.org\/10.1016\/j.jbiotec.2009.12.006","journal-title":"J Biotechnol"},{"issue":"2","key":"9296_CR13","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1093\/dnares\/dsq004","volume":"17","author":"T Fujisawa","year":"2010","unstructured":"Fujisawa T, Narikawa R, Okamoto S, Ehira S, Yoshimura H, Suzuki I, Masuda T, Mochimaru M, Takaichi S, Awai K, Sekine M, Horikawa H, Yashiro I, Omata S, Takarada H, Katano Y, Kosugi H, Tanikawa S, Ohmori K, Sato N, Ikeuchi M, Fujita N, Ohmori M (2010) Genomic structure of an economically important cyanobacterium, Arthrospira (Spirulina) platensis NIES-39. DNA Res 17(2):85\u2013103. \n                    https:\/\/doi.org\/10.1093\/dnares\/dsq004","journal-title":"DNA Res"},{"issue":"1","key":"9296_CR14","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1128\/MMBR.67.1.16-37.2003","volume":"67","author":"SB Gelvin","year":"2003","unstructured":"Gelvin SB (2003) Agrobacterium-mediated plant transformation: the biology behind the \u201cgene-jockeying\u201d tool. Microbiol Mol Biol Rev 67(1):16\u201337","journal-title":"Microbiol Mol Biol Rev"},{"issue":"12","key":"9296_CR15","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1007\/bf00235354","volume":"9","author":"I Godwin","year":"1991","unstructured":"Godwin I, Todd G, Ford-Lloyd B, Newbury HJ (1991) The effects of acetosyringone and pH on Agrobacterium-mediated transformation vary according to plant species. Plant Cell Rep 9(12):671\u2013675. \n                    https:\/\/doi.org\/10.1007\/bf00235354","journal-title":"Plant Cell Rep"},{"issue":"12","key":"9296_CR16","doi-asserted-by":"publisher","first-page":"1879","DOI":"10.1007\/s10295-011-1032-6","volume":"38","author":"Y Gong","year":"2011","unstructured":"Gong Y, Hu H, Gao Y, Xu X, Gao H (2011) Microalgae as platforms for production of recombinant proteins and valuable compounds: progress and prospects. J Ind Microbiol Biotechnol 38(12):1879\u20131890. \n                    https:\/\/doi.org\/10.1007\/s10295-011-1032-6","journal-title":"J Ind Microbiol Biotechnol"},{"issue":"12","key":"9296_CR17","doi-asserted-by":"publisher","first-page":"e28424","DOI":"10.1371\/journal.pone.0028424","volume":"6","author":"F Hempel","year":"2011","unstructured":"Hempel F, Lau J, Klingl A, Maier UG (2011) Algae as protein factories: expression of a human antibody and the respective antigen in the diatom Phaeodactylum tricornutum. PLoS One 6(12):e28424. \n                    https:\/\/doi.org\/10.1371\/journal.pone.0028424","journal-title":"PLoS One"},{"issue":"4","key":"9296_CR18","doi-asserted-by":"publisher","first-page":"822","DOI":"10.1093\/pcp\/pcx016","volume":"58","author":"W Jeamton","year":"2017","unstructured":"Jeamton W, Dulsawat S, Tanticharoen M, Vonshak A, Cheevadhanarak S (2017) Overcoming intrinsic restriction enzyme barriers enhances transformation efficiency in Arthrospira platensis C1. Plant Cell Physiol 58(4):822\u2013830. \n                    https:\/\/doi.org\/10.1093\/pcp\/pcx016","journal-title":"Plant Cell Physiol"},{"issue":"3","key":"9296_CR19","doi-asserted-by":"publisher","first-page":"642","DOI":"10.1111\/j.1529-8817.2009.00688.x","volume":"45","author":"S Kathiresan","year":"2009","unstructured":"Kathiresan S, Chandrashekar A, Ravishankar GA, Sarada R (2009) Agrobacterium-mediated transformation in the green alga Haematococcus pluvialis (Chlorophyceae, Volvocales). J Phycol 45(3):642\u2013649. \n                    https:\/\/doi.org\/10.1111\/j.1529-8817.2009.00688.x","journal-title":"J Phycol"},{"issue":"5","key":"9296_CR20","doi-asserted-by":"publisher","first-page":"1985","DOI":"10.1093\/nar\/14.5.1985","volume":"14","author":"M Kawamura","year":"1986","unstructured":"Kawamura M, Sakakibara M, Watanabe T, Kita K, Hiraoka N, Obayashi A, Takagi M, Yano K (1986) A new restriction endonuclease from Spirulina platensis. Nucleic Acids Res 14(5):1985\u20131989","journal-title":"Nucleic Acids Res"},{"issue":"4","key":"9296_CR21","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1007\/s10126-003-0037-1","volume":"6","author":"Y Kawata","year":"2004","unstructured":"Kawata Y, Yano S, Kojima H, Toyomizu M (2004) Transformation of Spirulina platensis strain C1 (Arthrospira sp. PCC9438) with Tn5 transposase-transposon DNA-cation liposome complex. Mar Biotechnol 6(4):355\u2013363. \n                    https:\/\/doi.org\/10.1007\/s10126-003-0037-1","journal-title":"Mar Biotechnol"},{"issue":"5","key":"9296_CR22","doi-asserted-by":"publisher","first-page":"373","DOI":"10.2174\/138920105774370607","volume":"6","author":"Z Khan","year":"2005","unstructured":"Khan Z, Bhadouria P, Bisen PS (2005) Nutritional and therapeutic potential of Spirulina. Curr Pharm Biotechnol 6(5):373\u2013379. \n                    https:\/\/doi.org\/10.2174\/138920105774370607","journal-title":"Curr Pharm Biotechnol"},{"issue":"7","key":"9296_CR23","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1111\/j.1365-2443.2012.01612.x","volume":"17","author":"K Kiyokawa","year":"2012","unstructured":"Kiyokawa K, Yamamoto S, Sato Y, Momota N, Tanaka K, Moriguchi K, Suzuki K (2012) Yeast transformation mediated by Agrobacterium strains harboring an Ri plasmid: comparative study between GALLS of an Ri plasmid and virE of a Ti plasmid. Genes Cells 17(7):597\u2013610. \n                    https:\/\/doi.org\/10.1111\/j.1365-2443.2012.01612.x","journal-title":"Genes Cells"},{"issue":"5","key":"9296_CR24","doi-asserted-by":"publisher","first-page":"400","DOI":"10.2174\/138920108785915111","volume":"9","author":"A Kulshreshtha","year":"2008","unstructured":"Kulshreshtha A, Zacharia AJ, Jarouliya U, Bhadauriya P, Prasad GB, Bisen PS (2008) Spirulina in health care management. Curr Pharm Biotechnol 9(5):400\u2013405. \n                    https:\/\/doi.org\/10.2174\/138920108785915111","journal-title":"Curr Pharm Biotechnol"},{"issue":"4","key":"9296_CR25","doi-asserted-by":"publisher","first-page":"1871","DOI":"10.1073\/pnas.041327598","volume":"98","author":"T Kunik","year":"2001","unstructured":"Kunik T, Tzfira T, Kapulnik Y, Gafni Y, Dingwall C, Citovsky V (2001) Genetic transformation of HeLa cells by Agrobacterium. Proc Natl Acad Sci U S A 98(4):1871\u20131876. \n                    https:\/\/doi.org\/10.1073\/pnas.041327598","journal-title":"Proc Natl Acad Sci U S A"},{"key":"9296_CR26","doi-asserted-by":"publisher","unstructured":"Lefort F, Calmin G, Crovadore J, Falquet J, Hurni JP, Osteras M, Haldemann F, Farinelli L (2014) Whole-genome shotgun sequence of Arthrospira platensis strain Paraca, a cultivated and edible cyanobacterium. Genome Announc 2(4). \n                    https:\/\/doi.org\/10.1128\/genomeA.00751-14","DOI":"10.1128\/genomeA.00751-14"},{"issue":"4","key":"9296_CR27","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1093\/oxfordjournals.molbev.a025615","volume":"13","author":"AR Lohe","year":"1996","unstructured":"Lohe AR, Hartl DL (1996) Autoregulation of mariner transposase activity by overproduction and dominant-negative complementation. Mol Biol Evol 13(4):549\u2013555. \n                    https:\/\/doi.org\/10.1093\/oxfordjournals.molbev.a025615","journal-title":"Mol Biol Evol"},{"issue":"8","key":"9296_CR28","doi-asserted-by":"publisher","first-page":"592","DOI":"10.1038\/nri3251","volume":"12","author":"N Lycke","year":"2012","unstructured":"Lycke N (2012) Recent progress in mucosal vaccine development: potential and limitations. Nat Rev Immunol 12(8):592\u2013605. \n                    https:\/\/doi.org\/10.1038\/nri3251","journal-title":"Nat Rev Immunol"},{"issue":"3","key":"9296_CR29","doi-asserted-by":"publisher","first-page":"466","DOI":"10.1007\/bf00218450","volume":"43","author":"G Mahajan","year":"1995","unstructured":"Mahajan G, Kamat M (1995) \u03b3-Linolenic acid production from Spirulina platensis. Appl Microbiol Biotechnol 43(3):466\u2013469. \n                    https:\/\/doi.org\/10.1007\/bf00218450","journal-title":"Appl Microbiol Biotechnol"},{"key":"9296_CR30","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1146\/annurev.cellbio.22.011105.102022","volume":"22","author":"CA McCullen","year":"2006","unstructured":"McCullen CA, Binns AN (2006) Agrobacterium tumefaciens and plant cell interactions and activities required for interkingdom macromolecular transfer. Annu Rev Cell Dev Biol 22:101\u2013127. \n                    https:\/\/doi.org\/10.1146\/annurev.cellbio.22.011105.102022","journal-title":"Annu Rev Cell Dev Biol"},{"issue":"3","key":"9296_CR31","doi-asserted-by":"publisher","first-page":"149","DOI":"10.5681\/bi.2011.020","volume":"1","author":"M Mohseniazar","year":"2011","unstructured":"Mohseniazar M, Barin M, Zarredar H, Alizadeh S, Shanehbandi D (2011) Potential of microalgae and lactobacilli in biosynthesis of silver nanoparticles. Bioimpacts 1(3):149\u2013152. \n                    https:\/\/doi.org\/10.5681\/bi.2011.020","journal-title":"Bioimpacts"},{"issue":"1513","key":"9296_CR32","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1098\/rstb.2008.0168","volume":"364","author":"DJ Obbard","year":"2009","unstructured":"Obbard DJ, Gordon KH, Buck AH, Jiggins FM (2009) The evolution of RNAi as a defence against viruses and transposable elements. Philos Trans R Soc Lond Ser B Biol Sci 364(1513):99\u2013115. \n                    https:\/\/doi.org\/10.1098\/rstb.2008.0168","journal-title":"Philos Trans R Soc Lond Ser B Biol Sci"},{"issue":"12","key":"9296_CR33","doi-asserted-by":"publisher","first-page":"e83896","DOI":"10.1371\/journal.pone.0083896","volume":"8","author":"SY Park","year":"2013","unstructured":"Park SY, Jeong MH, Wang HY, Kim JA, Yu NH, Kim S, Cheong YH, Kang S, Lee YH, Hur JS (2013) Agrobacterium tumefaciens-mediated transformation of the lichen fungus, Umbilicaria muehlenbergii. PLoS One 8(12):e83896. \n                    https:\/\/doi.org\/10.1371\/journal.pone.0083896","journal-title":"PLoS One"},{"issue":"6","key":"9296_CR34","doi-asserted-by":"publisher","first-page":"632","DOI":"10.1038\/sj.embor.7400165","volume":"5","author":"P Pelczar","year":"2004","unstructured":"Pelczar P, Kalck V, Gomez D, Hohn B (2004) Agrobacterium proteins VirD2 and VirE2 mediate precise integration of synthetic T-DNA complexes in mammalian cells. EMBO Rep 5(6):632\u2013637. \n                    https:\/\/doi.org\/10.1038\/sj.embor.7400165","journal-title":"EMBO Rep"},{"issue":"6","key":"9296_CR35","doi-asserted-by":"publisher","first-page":"910","DOI":"10.1016\/j.biotechadv.2010.08.006","volume":"28","author":"G Potvin","year":"2010","unstructured":"Potvin G, Zhang Z (2010) Strategies for high-level recombinant protein expression in transgenic microalgae: a review. Biotechnol Adv 28(6):910\u2013918. \n                    https:\/\/doi.org\/10.1016\/j.biotechadv.2010.08.006","journal-title":"Biotechnol Adv"},{"key":"9296_CR36","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.biologicals.2017.10.003","volume":"51","author":"Mohammad Mostafa Pourseif","year":"2018","unstructured":"Pourseif MM, Moghaddam G, Saeedi N, Barzegari A, Dehghani J, Omidi Y (2018) Current status and future prospective of vaccine development against Echinococcus granulosus. Biologicals 51:1\u201311. \n                    https:\/\/doi.org\/10.1016\/j.biologicals.2017.10.003","journal-title":"Biologicals"},{"issue":"20","key":"9296_CR37","doi-asserted-by":"publisher","first-page":"8629","DOI":"10.1007\/s00253-014-5900-7","volume":"98","author":"B Prasad","year":"2014","unstructured":"Prasad B, Vadakedath N, Jeong HJ, General T, Cho MG, Lein W (2014) Agrobacterium tumefaciens-mediated genetic transformation of haptophytes (Isochrysis species). Appl Microbiol Biotechnol 98(20):8629\u20138639. \n                    https:\/\/doi.org\/10.1007\/s00253-014-5900-7","journal-title":"Appl Microbiol Biotechnol"},{"issue":"6","key":"9296_CR38","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1007\/s12033-013-9720-2","volume":"56","author":"PT Pratheesh","year":"2014","unstructured":"Pratheesh PT, Vineetha M, Kurup GM (2014) An efficient protocol for the Agrobacterium-mediated genetic transformation of microalga Chlamydomonas reinhardtii. Mol Biotechnol 56(6):507\u2013515. \n                    https:\/\/doi.org\/10.1007\/s12033-013-9720-2","journal-title":"Mol Biotechnol"},{"issue":"6","key":"9296_CR39","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1007\/s00253-004-1647-x","volume":"65","author":"O Pulz","year":"2004","unstructured":"Pulz O, Gross W (2004) Valuable products from biotechnology of microalgae. Appl Microbiol Biotechnol 65(6):635\u2013648. \n                    https:\/\/doi.org\/10.1007\/s00253-004-1647-x","journal-title":"Appl Microbiol Biotechnol"},{"key":"9296_CR40","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1385\/1-59745-131-2:421","volume":"344","author":"MV Rajam","year":"2006","unstructured":"Rajam MV, Kumar SV (2006) Green alga (Chlamydomonas reinhardtii). Methods Mol Biol 344:421\u2013433. \n                    https:\/\/doi.org\/10.1385\/1-59745-131-2:421","journal-title":"Methods Mol Biol"},{"issue":"8","key":"9296_CR41","doi-asserted-by":"publisher","first-page":"e43349","DOI":"10.1371\/journal.pone.0043349","volume":"7","author":"BA Rasala","year":"2012","unstructured":"Rasala BA, Lee PA, Shen Z, Briggs SP, Mendez M, Mayfield SP (2012) Robust expression and secretion of Xylanase1 in Chlamydomonas reinhardtii by fusion to a selection gene and processing with the FMDV 2A peptide. PLoS One 7(8):e43349. \n                    https:\/\/doi.org\/10.1371\/journal.pone.0043349","journal-title":"PLoS One"},{"issue":"3","key":"9296_CR42","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1038\/cmi.2010.76","volume":"8","author":"C Selmi","year":"2011","unstructured":"Selmi C, Leung PS, Fischer L, German B, Yang CY, Kenny TP, Cysewski GR, Gershwin ME (2011) The effects of Spirulina on anemia and immune function in senior citizens. Cell Mol Immunol 8(3):248\u2013254. \n                    https:\/\/doi.org\/10.1038\/cmi.2010.76","journal-title":"Cell Mol Immunol"},{"issue":"4","key":"9296_CR43","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1007\/bf00021308","volume":"8","author":"SN Sheikholeslam","year":"1987","unstructured":"Sheikholeslam SN, Weeks DP (1987) Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens. Plant Mol Biol 8(4):291\u2013298. \n                    https:\/\/doi.org\/10.1007\/bf00021308","journal-title":"Plant Mol Biol"},{"issue":"4","key":"9296_CR44","doi-asserted-by":"publisher","first-page":"782","DOI":"10.1271\/bbb.120919","volume":"77","author":"H Shiraishi","year":"2013","unstructured":"Shiraishi H, Tabuse Y (2013) The AplI restriction-modification system in an edible cyanobacterium, Arthrospira (Spirulina) platensis NIES-39, recognizes the nucleotide sequence 5'-CTGCAG-3\u2032. Biosci Biotechnol Biochem 77(4):782\u2013788. \n                    https:\/\/doi.org\/10.1271\/bbb.120919","journal-title":"Biosci Biotechnol Biochem"},{"key":"9296_CR45","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1016\/j.algal.2015.06.007","volume":"11","author":"DP Simon","year":"2015","unstructured":"Simon DP, Narayanan A, Mallikarjun Gouda KG, Sarada R (2015) Vir gene inducers in Dunaliella salina; an insight in to the Agrobacterium-mediated genetic transformation of microalgae. Algal Res 11:121\u2013124. \n                    https:\/\/doi.org\/10.1016\/j.algal.2015.06.007","journal-title":"Algal Res"},{"key":"9296_CR46","doi-asserted-by":"publisher","first-page":"60","DOI":"10.3389\/fmicb.2014.00060","volume":"5","author":"EA Specht","year":"2014","unstructured":"Specht EA, Mayfield SP (2014) Algae-based oral recombinant vaccines. Front Microbiol 5:60. \n                    https:\/\/doi.org\/10.3389\/fmicb.2014.00060","journal-title":"Front Microbiol"},{"issue":"10","key":"9296_CR47","doi-asserted-by":"publisher","first-page":"1373","DOI":"10.1007\/s10529-010-0326-5","volume":"32","author":"E Specht","year":"2010","unstructured":"Specht E, Miyake-Stoner S, Mayfield S (2010) Microalgae come of age as a platform for recombinant protein production. Biotechnol Lett 32(10):1373\u20131383. \n                    https:\/\/doi.org\/10.1007\/s10529-010-0326-5","journal-title":"Biotechnol Lett"},{"issue":"2","key":"9296_CR48","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1263\/jbb.101.87","volume":"101","author":"P Spolaore","year":"2006","unstructured":"Spolaore P, Joannis-Cassan C, Duran E, Isambert A (2006) Commercial applications of microalgae. J Biosci Bioeng 101(2):87\u201396. \n                    https:\/\/doi.org\/10.1263\/jbb.101.87","journal-title":"J Biosci Bioeng"},{"issue":"3","key":"9296_CR49","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1023\/a:1011182613761","volume":"13","author":"M Toyomizu","year":"2001","unstructured":"Toyomizu M, Suzuki K, Kawata Y, Kojima H, Akiba Y (2001) Effective transformation of the cyanobacterium Spirulina platensis using electroporation. J Appl Phycol 13(3):209\u2013214. \n                    https:\/\/doi.org\/10.1023\/a:1011182613761","journal-title":"J Appl Phycol"},{"issue":"6","key":"9296_CR50","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1007\/bf00003943","volume":"7","author":"V Tragut","year":"1995","unstructured":"Tragut V, Xiao J, Bylina EJ, Borthakur D (1995) Characterization of DNA restriction-modification systems in Spirulina platensis strain pacifica. J Appl Phycol 7(6):561\u2013564. \n                    https:\/\/doi.org\/10.1007\/bf00003943","journal-title":"J Appl Phycol"},{"issue":"2","key":"9296_CR51","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1002\/bit.260250204","volume":"25","author":"A Vonshak","year":"1983","unstructured":"Vonshak A, Boussiba S, Abeliovich A, Richmond A (1983) Production of Spirulina biomass: maintenance of monoalgal culture outdoors. Biotechnol Bioeng 25(2):341\u2013349. \n                    https:\/\/doi.org\/10.1002\/bit.260250204","journal-title":"Biotechnol Bioeng"},{"issue":"2","key":"9296_CR52","doi-asserted-by":"publisher","first-page":"83","DOI":"10.5681\/bi.2012.012","volume":"2","author":"A Yari Khosroushahi","year":"2012","unstructured":"Yari Khosroushahi A (2012) Applications of diatoms as potential micro algae in Nanobiotechnology. Bioimpacts 2(2):83\u201389. \n                    https:\/\/doi.org\/10.5681\/bi.2012.012","journal-title":"Bioimpacts"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00253-018-9296-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-018-9296-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-018-9296-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,23]],"date-time":"2019-09-23T01:37:02Z","timestamp":1569202622000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00253-018-9296-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,29]]},"references-count":52,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["9296"],"URL":"https:\/\/doi.org\/10.1007\/s00253-018-9296-7","relation":{},"ISSN":["0175-7598","1432-0614"],"issn-type":[{"value":"0175-7598","type":"print"},{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,29]]},"assertion":[{"value":"3 June 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 July 2018","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 August 2018","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 August 2018","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"This article does not contain any studies with human participants or animals performed by any of the authors.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}]}}