{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T02:34:05Z","timestamp":1789785245511,"version":"4.0.1"},"reference-count":33,"publisher":"Springer Science and Business Media LLC","issue":"9","license":[{"start":{"date-parts":[[2020,3,13]],"date-time":"2020-03-13T00:00:00Z","timestamp":1584057600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,3,13]],"date-time":"2020-03-13T00:00:00Z","timestamp":1584057600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"published-print":{"date-parts":[[2020,5]]},"DOI":"10.1007\/s00253-020-10490-6","type":"journal-article","created":{"date-parts":[[2020,3,13]],"date-time":"2020-03-13T06:35:31Z","timestamp":1584081331000},"page":"4127-4139","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Optimization and validation of a blocking ELISA for quantitation of anti-rabies immunoglobulins in multispecies sera"],"prefix":"10.1007","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5109-7637","authenticated-orcid":false,"given":"Diego","family":"Fontana","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7640-6221","authenticated-orcid":false,"given":"Mar\u00eda Celeste","family":"Rodriguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernesto","family":"Garay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Russo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio","family":"Prieto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,3,13]]},"reference":[{"key":"10490_CR1","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1016\/S0195-5616(01)50610-5","volume":"31","author":"DJ Briggs","year":"1997","unstructured":"Briggs DJ, Schweitzer K (1997) Importation of dogs and cats to rabies-free areas of the world. Vet Clin North Am Small Anim Pract 31:573\u2013583. https:\/\/doi.org\/10.1016\/S0195-5616(01)50610-5","journal-title":"Vet Clin North Am Small Anim Pract"},{"key":"10490_CR2","doi-asserted-by":"publisher","first-page":"3128","DOI":"10.1128\/JVI.62.9.3128-3134.1988","volume":"62","author":"E Celis","year":"1988","unstructured":"Celis E, Ou D, Dietzschold B, Koprowski H (1988) Recognition of rabies and rabies-related viruses by T cells derived from human vaccine recipients. J Virol 62:3128\u20133134","journal-title":"J Virol"},{"key":"10490_CR3","doi-asserted-by":"publisher","unstructured":"Cliquet F, Aubert M, Sag L (1998) Development of a fluorescent antibody virus neutralisation test (FAVN test) for the quantitation of rabies-neutralising antibody. 212:79\u201387. https:\/\/doi.org\/10.1016\/s0022-1759(97)00212-3","DOI":"10.1016\/s0022-1759(97)00212-3"},{"key":"10490_CR4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jviromet.2003.12.001","volume":"117","author":"F Cliquet","year":"2004","unstructured":"Cliquet F, McElhinney LM, Servat A, Boucher JM, Lowings JP, Goddard T, Mansfield KL, Fooks AR (2004) Development of a qualitative indirect ELISA for the measurement of rabies virus-specific antibodies from vaccinated dogs and cats. J Virol Methods 117:1\u20138. https:\/\/doi.org\/10.1016\/j.jviromet.2003.12.001","journal-title":"J Virol Methods"},{"key":"10490_CR5","doi-asserted-by":"publisher","first-page":"754","DOI":"10.1128\/IAI.16.3.754-759.1977","volume":"16","author":"JH Cox","year":"1977","unstructured":"Cox JH, Dietzschold B, Schneider LG (1977) Rabies virus glycoprotein. II. Biological and serological characterization. Infect Immun 16:754\u2013759","journal-title":"Infect Immun"},{"key":"10490_CR6","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1080\/00224065.1980.11980968","volume":"12","author":"G Derringer","year":"1980","unstructured":"Derringer G, Suich R (1980) Simultaneous optimization of several response variables. J Qual Technol 12:214\u2013219. https:\/\/doi.org\/10.1080\/00224065.1980.11980968","journal-title":"J Qual Technol"},{"key":"10490_CR7","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000515","volume":"3","author":"HCJ Ertl","year":"2009","unstructured":"Ertl HCJ (2009) Novel vaccines to human rabies. PLoS Negl Trop Dis 3:e515. https:\/\/doi.org\/10.1371\/journal.pntd.0000515","journal-title":"PLoS Negl Trop Dis"},{"key":"10490_CR8","unstructured":"FDA (2018) Bioanalytical method validation guidance for industry bioanalytical method validation guidance for industry. https:\/\/www.fda.gov\/files\/drugs\/published\/Bioanalytical-Method-Validation-Guidance-for-Industry.pdf"},{"key":"10490_CR9","doi-asserted-by":"crossref","unstructured":"Fehlner-gardiner C, Wandeler AI (2015) Detection of rabies virus antibodies by competitive enzyme-linked immunosorbent assay, 2nd edn, Elsevier Inc","DOI":"10.1016\/B978-0-12-801919-1.00013-0"},{"key":"10490_CR10","doi-asserted-by":"publisher","unstructured":"Ferreira SLC, Bruns RE, Ferreira HS, Matos GD, David JM, Brandao GC, da Silva E, Portugal L, dos Reis P, Souza A, dos Santos W (2007) Box-Behnken design: An alternative for the optimizacion of analytical methods. Anal Chim Acta 597:179\u2013186 https:\/\/doi.org\/10.1016\/j.aca.2007.07.011","DOI":"10.1016\/j.aca.2007.07.011"},{"key":"10490_CR11","doi-asserted-by":"publisher","first-page":"2244","DOI":"10.1016\/j.vaccine.2006.12.012","volume":"25","author":"M Feyssaguet","year":"2007","unstructured":"Feyssaguet M, Dacheux L, Audry L, Compoint A, Morize JL, Blanchard I, Bourhy H (2007) Multicenter comparative study of a new ELISA, PLATELIA RABIES II, for the detection and titration of anti-rabies glycoprotein antibodies and comparison with the rapid fluorescent focus inhibition test (RFFIT) on human samples from vaccinated and non-vacci. Vaccine 25:2244\u20132251. https:\/\/doi.org\/10.1016\/j.vaccine.2006.12.012","journal-title":"Vaccine"},{"key":"10490_CR12","doi-asserted-by":"publisher","unstructured":"Fontana D, Kratje R, Etcheverrigaray M, Prieto C (2014) Rabies virus-like particles expressed in HEK293 cells. Vaccine. https:\/\/doi.org\/10.1016\/j.vaccine.2014.02.031","DOI":"10.1016\/j.vaccine.2014.02.031"},{"key":"10490_CR13","doi-asserted-by":"publisher","unstructured":"Fontana D, Kratje R, Etcheverrigaray M, Prieto C (2015) Immunogenic virus-like particles continuously expressed in mammalian cells as a veterinary rabies vaccine candidate. Vaccine. https:\/\/doi.org\/10.1016\/j.vaccine.2015.03.088","DOI":"10.1016\/j.vaccine.2015.03.088"},{"key":"10490_CR14","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1016\/j.cimid.2019.04.009","volume":"65","author":"D Fontana","year":"2019","unstructured":"Fontana D, Marsili F, Garay E, Battagliotti J, Etcheverrigaray M, Kratje R, Prieto C (2019) A simplified roller bottle platform for the production of a new generation VLPs rabies vaccine for veterinary applications. Comp Immunol Microbiol Infect Dis 65:70\u201375. https:\/\/doi.org\/10.1016\/j.cimid.2019.04.009","journal-title":"Comp Immunol Microbiol Infect Dis"},{"key":"10490_CR15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.5334\/aogh.2334","volume":"85","author":"JA Galhardo","year":"2019","unstructured":"Galhardo JA, de Azevedo CS, Remonti BR, Neres Gon\u00e7alves VM, Azevedo Marques NT, Borges LO, Ahad das Neves D (2019) Canine rabies in the Brazil-Bolivia border region from 2006 to 2014. Ann Glob Health 85:1\u20135. https:\/\/doi.org\/10.5334\/aogh.2334","journal-title":"Ann Glob Health"},{"key":"10490_CR16","unstructured":"ICH (2005) ICH. Validation of analytical procedures: text and methodology Q2 (R1). 1\u201313. https:\/\/www.ema.europa.eu\/en\/documents\/scientific-guideline\/ich-q-2-r1-validation-analytical-procedures-text-methodology-step-5_en.pdf"},{"key":"10490_CR17","unstructured":"International Committee on Taxonomy of Viruses (2019) Online 10th Report, https:\/\/ictv.global\/report\/ Accesed 15 January 2020"},{"key":"10490_CR18","doi-asserted-by":"publisher","first-page":"27","DOI":"10.24070\/bjvp.1983-0246.v12i2p27-32","volume":"12","author":"G J\u00e1uregui","year":"2019","unstructured":"J\u00e1uregui G, Fontana D, Micheloud J, Prieto C, Delgado F (2019) Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples. Brazilian J Vet Pathol 12:27\u201332. https:\/\/doi.org\/10.24070\/bjvp.1983-0246.v12i2p27-32","journal-title":"Brazilian J Vet Pathol"},{"key":"10490_CR19","doi-asserted-by":"publisher","first-page":"3310","DOI":"10.1016\/j.vaccine.2016.04.086","volume":"34","author":"J Korimbocus","year":"2016","unstructured":"Korimbocus J, Dehay N, Tordo N, Cano F, Morgeaux S (2016) Development and validation of a quantitative competitive ELISA for potency testing of equine anti rabies sera with other potential use. Vaccine 34:3310\u20133316. https:\/\/doi.org\/10.1016\/j.vaccine.2016.04.086","journal-title":"Vaccine"},{"key":"10490_CR20","doi-asserted-by":"publisher","unstructured":"Laemmli, U (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680\u2013685. https:\/\/doi.org\/10.1038\/227680a0","DOI":"10.1038\/227680a0"},{"key":"10490_CR21","doi-asserted-by":"publisher","unstructured":"Leardi R (2009) Experimental design in chemistry: A tutorial. Anal Chim Acta 652(1):161\u2013172. https:\/\/doi.org\/10.1016\/j.aca.2009.06.015","DOI":"10.1016\/j.aca.2009.06.015"},{"key":"10490_CR22","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000595","volume":"4","author":"SM Moore","year":"2010","unstructured":"Moore SM, Hanlon CA (2010) Rabies-specific antibodies: measuring surrogates of protection against a fatal disease. PLoS Negl Trop Dis 4:e595. https:\/\/doi.org\/10.1371\/journal.pntd.0000595","journal-title":"PLoS Negl Trop Dis"},{"key":"10490_CR23","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1016\/B978-0-12-396547-9.00012-2","volume-title":"Rabies","author":"SM Moore","year":"2013","unstructured":"Moore SM, Gordon CR, Hanlon CA (2013) Measures of rabies immunity. In: Jackson AC (ed) Rabies, 3rd edn. Elsevier, San Diego, pp 461\u2013496","edition":"3"},{"key":"10490_CR24","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1002\/jmv.1890090107","volume":"9","author":"KG Nicholson","year":"1982","unstructured":"Nicholson KG, Prestage H (1982) Enzyme-linked immunosorbent assay: a rapid reproducible test for the measurement of rabies antibody. J Med Virol 9:43\u201349. https:\/\/doi.org\/10.1002\/jmv.1890090107","journal-title":"J Med Virol"},{"key":"10490_CR25","doi-asserted-by":"publisher","unstructured":"Ockuly RA, Weese ML, Smucker BJ, Edwards DJ, Chang L (2017) Response surface experiments: A meta-analysis. Chemom. Intell Lab Syst 154:64\u201375. https:\/\/doi.org\/10.1016\/j.chemolab.2017.03.009","DOI":"10.1016\/j.chemolab.2017.03.009"},{"key":"10490_CR26","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.aca.2015.01.017","volume":"868","author":"AC Olivieri","year":"2015","unstructured":"Olivieri AC (2015) Practical guidelines for reporting results in single- and multi-component analytical calibration: a tutorial. Anal Chim Acta 868:10\u201322. https:\/\/doi.org\/10.1016\/j.aca.2015.01.017","journal-title":"Anal Chim Acta"},{"key":"10490_CR27","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1038\/nrmicro2260","volume":"8","author":"MJ Schnell","year":"2010","unstructured":"Schnell MJ, McGettigan JP, Wirblich C, Papaneri A (2010) The cell biology of rabies virus: using stealth to reach the brain. Nat Rev Microbiol 8:51\u201361. https:\/\/doi.org\/10.1038\/nrmicro2260","journal-title":"Nat Rev Microbiol"},{"key":"10490_CR28","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jim.2006.07.026","volume":"318","author":"A Servat","year":"2007","unstructured":"Servat A, Feyssaguet M, Blanchard I, Morize JL, Schereffer JL, Boue F, Cliquet F (2007) A quantitative indirect ELISA to monitor the effectiveness of rabies vaccination in domestic and wild carnivores. J Immunol Methods 318:1\u201310. https:\/\/doi.org\/10.1016\/j.jim.2006.07.026","journal-title":"J Immunol Methods"},{"key":"10490_CR29","first-page":"535","volume":"48","author":"JS Smith","year":"1973","unstructured":"Smith JS, Yager PA, Baer GM (1973) A rapid reproducible test for determining rabies neutralizing antibody. Bull World Health Organ 48:535\u2013541","journal-title":"Bull World Health Organ"},{"key":"10490_CR30","doi-asserted-by":"publisher","unstructured":"Vanaja K, Rani RHS (2007) Design of experiments: concept and applications of plackett burman design. Res Regul Aff 24(1):1\u201323. https:\/\/doi.org\/10.1080\/10601330701220520","DOI":"10.1080\/10601330701220520"},{"key":"10490_CR31","doi-asserted-by":"publisher","first-page":"570","DOI":"10.3181\/00379727-32-7767P","volume":"32","author":"LT Webster","year":"1935","unstructured":"Webster LT, Dawson JR (1935) Early diagnosis of rabies by mouse inoculation. Measurement of humoral immunity to rabies by mouse protection test. Proc Soc Exp Biol Med 32:570\u2013573. https:\/\/doi.org\/10.3181\/00379727-32-7767P","journal-title":"Proc Soc Exp Biol Med"},{"key":"10490_CR32","unstructured":"World Health Organization (2013) WHO Expert Consultation on rabies. https:\/\/apps.who.int\/iris\/handle\/10665\/85346"},{"key":"10490_CR33","unstructured":"World Health Organization (2018) WHO Expert Consultation on rabies. https:\/\/apps.who.int\/iris\/handle\/10665\/272364"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-020-10490-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00253-020-10490-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-020-10490-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,13]],"date-time":"2021-03-13T00:06:47Z","timestamp":1615594007000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00253-020-10490-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,13]]},"references-count":33,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2020,5]]}},"alternative-id":["10490"],"URL":"https:\/\/doi.org\/10.1007\/s00253-020-10490-6","relation":{},"ISSN":["0175-7598","1432-0614"],"issn-type":[{"value":"0175-7598","type":"print"},{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,13]]},"assertion":[{"value":"9 October 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 February 2020","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 February 2020","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 March 2020","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standard"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Informed consent was obtained from all volunteers according to the ethical guidelines for human subject research. The project was approved by the ethical committee from Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas of the Universidad Nacional del Litoral, Santa Fe, Argentina, and with the endorsement of the National Network of Rabies Diagnostic Laboratories (Argentina).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Research involving human participants and\/or animals"}}]}}