{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T05:25:09Z","timestamp":1789709109421,"version":"4.0.1"},"reference-count":87,"publisher":"Springer Science and Business Media LLC","issue":"12","license":[{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,6,15]],"date-time":"2021-06-15T00:00:00Z","timestamp":1623715200000},"content-version":"vor","delay-in-days":14,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100008751","name":"Bundesinstitut f\u00fcr Risikobewertung","doi-asserted-by":"publisher","award":["1322-686"],"award-info":[{"award-number":["1322-686"]}],"id":[{"id":"10.13039\/501100008751","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003107","name":"Bundesministerium f\u00fcr Gesundheit","doi-asserted-by":"publisher","award":["ZMVI1-2516-AUK-701 \/ BMG: 321-4471-02\/157"],"award-info":[{"award-number":["ZMVI1-2516-AUK-701 \/ BMG: 321-4471-02\/157"]}],"id":[{"id":"10.13039\/501100003107","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009139","name":"DZIF","doi-asserted-by":"crossref","award":["TTU \"Emerging Infections\""],"award-info":[{"award-number":["TTU \"Emerging Infections\""]}],"id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:sec>\n                <jats:title>Abstract<\/jats:title>\n                <jats:p>To generate a hepatitis E virus (HEV) genotype 3 (HEV-3)\u2013specific monoclonal antibody (mAb), the <jats:italic>Escherichia coli<\/jats:italic>\u2013expressed carboxy-terminal part of its capsid protein was used to immunise BALB\/c mice. The immunisation resulted in the induction of HEV-specific antibodies of high titre. The mAb G117-AA4 of IgG1 isotype was obtained showing a strong reactivity with the homologous <jats:italic>E. coli<\/jats:italic>, but also yeast-expressed capsid protein of HEV-3. The mAb strongly cross-reacted with ratHEV capsid protein derivatives produced in both expression systems and weaker with an <jats:italic>E. coli<\/jats:italic>\u2013expressed batHEV capsid protein fragment. In addition, the mAb reacted with capsid protein derivatives of genotypes HEV-2 and HEV-4 and common vole hepatitis E virus (cvHEV), produced by the cell-free synthesis in Chinese hamster ovary (CHO) and <jats:italic>Spodoptera frugiperda<\/jats:italic> (<jats:italic>Sf<\/jats:italic>21) cell lysates. Western blot and line blot reactivity of the mAb with capsid protein derivatives of HEV-1 to HEV-4, cvHEV, ratHEV and batHEV suggested a linear epitope. Use of truncated derivatives of ratHEV capsid protein in ELISA, Western blot, and a Pepscan analysis allowed to map the epitope within a partially surface-exposed region with the amino acid sequence LYTSV. The mAb was also shown to bind to human patient\u2013derived HEV-3 from infected cell culture and to hare HEV-3 and camel HEV-7 capsid proteins from transfected cells by immunofluorescence assay. The novel mAb may serve as a useful tool for further investigations on the pathogenesis of HEV infections and might be used for diagnostic purposes.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Key points<\/jats:title>\n                <jats:p><jats:italic>\u2022 The antibody showed cross-reactivity with capsid proteins of different hepeviruses.<\/jats:italic><\/jats:p>\n                <jats:p><jats:italic>\u2022 The linear epitope of the antibody was mapped in a partially surface-exposed region.<\/jats:italic><\/jats:p>\n                <jats:p><jats:italic>\u2022 The antibody detected native HEV-3 antigen in infected mammalian cells.<\/jats:italic><\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s00253-021-11342-7","type":"journal-article","created":{"date-parts":[[2021,6,15]],"date-time":"2021-06-15T08:03:00Z","timestamp":1623744180000},"page":"4957-4973","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen"],"prefix":"10.1007","volume":"105","author":[{"given":"Barbara","family":"Kubickova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg A.","family":"Schenk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franziska","family":"Ramm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kornelija","family":"Marku\u0161kien\u0117","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jochen","family":"Reetz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Dremsek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulius Lukas","family":"Tamosiunas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laima","family":"Cepulyte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoai Anh","family":"Trinh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Scholz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry","family":"Memczak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Hovest\u00e4dt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9","family":"Ryll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasa","family":"Petraityte-Burneikiene","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor M.","family":"Corman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anika","family":"Andersson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dietmar","family":"Becher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin H.","family":"Groschup","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Kubick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Sellrie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reimar","family":"Johne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5620-1528","authenticated-orcid":false,"given":"Rainer G.","family":"Ulrich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,6,15]]},"reference":[{"key":"11342_CR1","first-page":"63","volume":"27","author":"SK Acharya","year":"2006","unstructured":"Acharya SK, Panda SK (2006) Hepatitis E virus: epidemiology, diagnosis, pathology and prevention. Trop Gastroenterol 27:63\u201368","journal-title":"Trop Gastroenterol"},{"issue":"6","key":"11342_CR2","doi-asserted-by":"publisher","first-page":"951","DOI":"10.1093\/infdis\/jiz025","volume":"220","author":"A Andonov","year":"2019","unstructured":"Andonov A, Robbins M, Borlang J, Cao J, Hatchette T, Stueck A, Deschambault Y, Murnaghan K, Varga J, Johnston L (2019) Rat hepatitis E virus linked to severe acute hepatitis in an immunocompetent patient. J Infect Dis 220(6):951\u2013955. https:\/\/doi.org\/10.1093\/infdis\/jiz025","journal-title":"J Infect Dis"},{"key":"11342_CR3","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1159\/000149370","volume":"20","author":"MS Balayan","year":"1983","unstructured":"Balayan MS, Andjaparidze AG, Savinskaya SS, Ketiladze ES, Braginsky DM, Savinov AP, Poleschuk VF (1983) Evidence for a virus in non-A, non-B hepatitis transmitted via the fecal-oral route. Intervirology 20:23\u201331. https:\/\/doi.org\/10.1159\/000149370","journal-title":"Intervirology"},{"key":"11342_CR4","doi-asserted-by":"publisher","unstructured":"Batts W, Yun S, Hedrick R, Winton J (2011) A novel member of the family Hepeviridae from cutthroat trout (Oncorhynchus clarkii). Virus Res 158:116\u2013123. https:\/\/doi.org\/10.1016\/j.virusres.2011.03.019","DOI":"10.1016\/j.virusres.2011.03.019"},{"key":"11342_CR5","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1016\/j.meegid.2015.06.026","volume":"34","author":"N Behloul","year":"2015","unstructured":"Behloul N, Wen J, Dai X, Dong C, Meng J (2015) Antigenic composition and immunoreactivity differences between HEV recombinant capsid proteins generated from different genotypes. Infect Genet Evol 34:211\u2013220. https:\/\/doi.org\/10.1016\/j.meegid.2015.06.026","journal-title":"Infect Genet Evol"},{"key":"11342_CR6","doi-asserted-by":"publisher","first-page":"264","DOI":"10.3201\/eid1902.121255","volume":"19","author":"A Berto","year":"2013","unstructured":"Berto A, Grierson S, Hakze-van der Honing R, Martelli F, Johne R, Reetz J, Pavio N, Van der Poel WHM, Banks M (2013) Hepatitis E virus in pork liver sausage, France. Emerg Infect Dis 19:264\u2013266","journal-title":"Emerg Infect Dis"},{"key":"11342_CR7","doi-asserted-by":"publisher","first-page":"7758","DOI":"10.1128\/JVI.00568-13","volume":"87","author":"R Bodewes","year":"2013","unstructured":"Bodewes R, van der Giessen J, Haagmans BL, Osterhaus ADME, Smits SL (2013) Identification of multiple novel viruses, including a parvovirus and a hepevirus, in feces of red foxes. J Virol 87:7758\u20137764. https:\/\/doi.org\/10.1128\/JVI.00568-13","journal-title":"J Virol"},{"key":"11342_CR8","volume-title":"Zakim and Boyer\u2019s hepatology: a textbook of liver disease","author":"TD Boyer","year":"2012","unstructured":"Boyer TD, Manns MP, Sanyal AJ (2012) Zakim and Boyer\u2019s hepatology: a textbook of liver disease. Saunders, Philadelphia"},{"issue":"12","key":"11342_CR9","doi-asserted-by":"publisher","first-page":"e82234","DOI":"10.1371\/journal.pone.0082234","volume":"8","author":"AK Br\u00f6del","year":"2013","unstructured":"Br\u00f6del AK, Sonnabend A, Roberts LO, Stech M, W\u00fcstenhagen DA, Kubick S (2013) IRES-mediated translation of membrane proteins and glycoproteins in eukaryotic cell-free systems. PLoS One 8(12):e82234. https:\/\/doi.org\/10.1371\/journal.pone.0082234","journal-title":"PLoS One"},{"issue":"1","key":"11342_CR10","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1002\/bit.25013","volume":"111","author":"AK Br\u00f6del","year":"2014","unstructured":"Br\u00f6del AK, Sonnabend A, Kubick S (2014) Cell-free protein expression based on extracts from CHO cells. Biotechnol Bioeng 111(1):25\u201336. https:\/\/doi.org\/10.1002\/bit.25013","journal-title":"Biotechnol Bioeng"},{"key":"11342_CR11","doi-asserted-by":"publisher","first-page":"e17","DOI":"10.1038\/emi.2012.7","volume":"1","author":"D Cao","year":"2012","unstructured":"Cao D, Meng X-J (2012) Molecular biology and replication of hepatitis E virus. Emerg Microbes Infect 1:e17. https:\/\/doi.org\/10.1038\/emi.2012.7","journal-title":"Emerg Microbes Infect"},{"issue":"6","key":"11342_CR12","doi-asserted-by":"publisher","first-page":"1233","DOI":"10.3201\/eid2506.181618","volume":"25","author":"VM Corman","year":"2019","unstructured":"Corman VM, Hilgensloh L, Voigt U, Marklewitz M, Siebert U, Drosten C, Drexler JF (2019) Hepatitis E virus infection in European brown hares, Germany, 2007-2014. Emerg Infect Dis 25(6):1233\u20131235. https:\/\/doi.org\/10.3201\/eid2506.181618","journal-title":"Emerg Infect Dis"},{"key":"11342_CR13","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1007\/s00430-011-0221-2","volume":"201","author":"P Dremsek","year":"2012","unstructured":"Dremsek P, Wenzel JJ, Johne R, Ziller M, Hofmann J, Groschup MH, Werdermann S, Mohn U, Dorn S, Motz M, Mertens M, Jilg W, Ulrich RG (2012) Seroprevalence study in forestry workers from eastern Germany using novel genotype 3- and rat hepatitis E virus-specific immunoglobulin G ELISAs. Med Microbiol Immunol 201:189\u2013200. https:\/\/doi.org\/10.1007\/s00430-011-0221-2","journal-title":"Med Microbiol Immunol"},{"key":"11342_CR14","doi-asserted-by":"publisher","unstructured":"Drexler JF, Seelen A, Corman VM, Fumie Tateno A, Cottontail V, Melim Zerbinati R, Gloza-Rausch F, Klose SM, Adu-Sarkodie Y, Oppong SK, Kalko EKV, Osterman A, Rasche A, Adam A, M\u00fcller MA, Ulrich RG, Leroy EM, Lukashev AN, Drosten C (2012) Bats worldwide carry hepatitis E virus-related viruses that form a putative novel genus within the family Hepeviridae. J Virol 86:9134\u20139147. https:\/\/doi.org\/10.1128\/JVI.00800-12","DOI":"10.1128\/JVI.00800-12"},{"key":"11342_CR15","doi-asserted-by":"publisher","first-page":"e24","DOI":"10.1086\/654801","volume":"51","author":"J Drobeniuc","year":"2010","unstructured":"Drobeniuc J, Meng J, Reuter G, Greene-Montfort T, Khudyakova N, Dimitrova Z, Kamili S, Teo C-G (2010) Serologic assays specific to immunoglobulin M antibodies against hepatitis E virus: pangenotypic evaluation of performances. Clin Infect Dis 51:e24\u2013e27. https:\/\/doi.org\/10.1086\/654801","journal-title":"Clin Infect Dis"},{"key":"11342_CR16","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1016\/j.tim.2013.10.005","volume":"22","author":"Z Feng","year":"2014","unstructured":"Feng Z, Lemon SM (2014) Peek-a-boo: membrane hijacking and the pathogenesis of viral hepatitis. Trends Microbiol 22:59\u201364. https:\/\/doi.org\/10.1016\/j.tim.2013.10.005","journal-title":"Trends Microbiol"},{"key":"11342_CR17","doi-asserted-by":"publisher","first-page":"9217","DOI":"10.1016\/S0040-4020(01)85612-X","volume":"48","author":"R Frank","year":"1992","unstructured":"Frank R (1992) Spot-synthesis: : an easy technique for the positionally addressable, parallel chemical synthesis on a membrane support. Tetrahedron 48:9217\u20139232. https:\/\/doi.org\/10.1016\/S0040-4020(01)85612-X","journal-title":"Tetrahedron"},{"key":"11342_CR18","volume-title":"Molecular cloning","author":"MR Green","year":"2012","unstructured":"Green MR, Sambrook J (2012) Molecular cloning, 4th edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor","edition":"4"},{"key":"11342_CR19","doi-asserted-by":"publisher","first-page":"1341","DOI":"10.3201\/eid1908.130069","volume":"19","author":"D Guan","year":"2013","unstructured":"Guan D, Li W, Su J, Fang L, Takeda N, Wakita T, Li T-C, Ke C (2013) Asian musk shrew as a reservoir of rat hepatitis E virus, China. Emerg Infect Dis 19:1341\u20131343. https:\/\/doi.org\/10.3201\/eid1908.130069","journal-title":"Emerg Infect Dis"},{"key":"11342_CR20","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1007\/s11373-007-9172-4","volume":"14","author":"J He","year":"2007","unstructured":"He J, Kuschner RA, Dewar V, Voet P, Asher LV, Vaughn DW (2007) Characterisation of monoclonal antibodies to hepatitis E virus (HEV) capsid protein and identification of binding activity. J Biomed Sci 14:555\u2013563. https:\/\/doi.org\/10.1007\/s11373-007-9172-4","journal-title":"J Biomed Sci"},{"key":"11342_CR21","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1128\/JVI.70.1.207-216.1996","volume":"70","author":"S Jameel","year":"1996","unstructured":"Jameel S, Zafrullah M, Ozdener MH, Panda SK (1996) Expression in animal cells and characterisation of the hepatitis E virus structural proteins. J Virol 70:207\u2013216. https:\/\/doi.org\/10.1128\/JVI.70.1.207-216.1996","journal-title":"J Virol"},{"key":"11342_CR22","doi-asserted-by":"publisher","first-page":"1452","DOI":"10.3201\/eid1609.100444","volume":"16","author":"R Johne","year":"2010","unstructured":"Johne R, Heckel G, Plenge-B\u00f6nig A, Kindler E, Maresch C, Reetz J, Schielke A, Ulrich RG (2010a) Novel hepatitis E virus genotype in Norway rats, Germany. Emerg Infect Dis 16:1452\u20131455. https:\/\/doi.org\/10.3201\/eid1609.100444","journal-title":"Emerg Infect Dis"},{"key":"11342_CR23","doi-asserted-by":"publisher","first-page":"750","DOI":"10.1099\/vir.0.016584-0","volume":"91","author":"R Johne","year":"2010","unstructured":"Johne R, Plenge-B\u00f6nig A, Hess M, Ulrich RG, Reetz J, Schielke A (2010b) Detection of a novel hepatitis E-like virus in faeces of wild rats using a nested broad-spectrum RT-PCR. J Gen Virol 91:750\u2013758. https:\/\/doi.org\/10.1099\/vir.0.016584-0","journal-title":"J Gen Virol"},{"key":"11342_CR24","doi-asserted-by":"publisher","first-page":"947","DOI":"10.1016\/j.meegid.2012.02.021","volume":"12","author":"R Johne","year":"2012","unstructured":"Johne R, Dremsek P, Kindler E, Schielke A, Plenge-B\u00f6nig A, Gregersen H, Wessels U, Schmidt K, Rietschel W, Groschup MH, Guenther S, Heckel G, Ulrich RG (2012) Rat hepatitis E virus: geographical clustering within Germany and serological detection in wild Norway rats (Rattus norvegicus). Infect Genet Evol 12:947\u2013956. https:\/\/doi.org\/10.1016\/j.meegid.2012.02.021","journal-title":"Infect Genet Evol"},{"key":"11342_CR25","doi-asserted-by":"publisher","unstructured":"Johne R, Dremsek P, Reetz J, Heckel G, Hess M, Ulrich RG (2014a) Hepeviridae: an expanding family of vertebrate viruses. Infect Genet Evol 27:212\u2013229. https:\/\/doi.org\/10.1016\/j.meegid.2014.06.024","DOI":"10.1016\/j.meegid.2014.06.024"},{"key":"11342_CR26","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1111\/jvh.12157","volume":"21","author":"R Johne","year":"2014","unstructured":"Johne R, Reetz J, Ulrich RG, Machnowska P, Sachsenr\u00f6der J, Nickel P, Hofmann J (2014b) An ORF1-rearranged hepatitis E virus derived from a chronically infected patient efficiently replicates in cell culture. J Viral Hepat 21:447\u2013456. https:\/\/doi.org\/10.1111\/jvh.12157","journal-title":"J Viral Hepat"},{"issue":"14","key":"11342_CR27","doi-asserted-by":"publisher","first-page":"4225","DOI":"10.1128\/AEM.00951-16","volume":"82","author":"R Johne","year":"2016","unstructured":"Johne R, Trojnar E, Filter M, Hofmann J (2016) Thermal Stability of Hepatitis E Virus as Estimated by a Cell Culture Method. Appl Environ Microbiol 82(14):4225\u20134231. https:\/\/doi.org\/10.1128\/AEM.00951-16","journal-title":"Appl Environ Microbiol"},{"key":"11342_CR28","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1016\/j.virusres.2011.06.006","volume":"161","author":"Y Khudyakov","year":"2011","unstructured":"Khudyakov Y, Kamili S (2011) Serological diagnostics of hepatitis E virus infection. Virus Res 161:84\u201392. https:\/\/doi.org\/10.1016\/j.virusres.2011.06.006","journal-title":"Virus Res"},{"key":"11342_CR29","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1016\/j.jviromet.2016.03.004","volume":"233","author":"T Kobayashi","year":"2016","unstructured":"Kobayashi T, Takahashi M, Tanggis, Mulyanto, Jirintai S, Nagashima S, Nishizawa T, Okamoto H (2016) Characterisation and epitope mapping of monoclonal antibodies raised against rat hepatitis E virus capsid protein: an evaluation of their neutralising activity in a cell culture system. J Virol Methods 233:78\u201388. https:\/\/doi.org\/10.1016\/j.jviromet.2016.03.004","journal-title":"J Virol Methods"},{"key":"11342_CR30","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1038\/256495a0","volume":"256","author":"G K\u00f6hler","year":"1975","unstructured":"K\u00f6hler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495\u2013497. https:\/\/doi.org\/10.1038\/256495a0","journal-title":"Nature"},{"key":"11342_CR31","doi-asserted-by":"publisher","first-page":"2028","DOI":"10.3201\/eid1912.130614","volume":"19","author":"JS Krog","year":"2013","unstructured":"Krog JS, Breum S\u00d8, Jensen TH, Larsen LE (2013) Hepatitis E virus variant in farmed mink, Denmark. Emerg Infect Dis 19:2028\u20132030. https:\/\/doi.org\/10.3201\/eid1912.130614","journal-title":"Emerg Infect Dis"},{"issue":"2","key":"11342_CR32","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1053\/j.gastro.2015.10.048","volume":"150","author":"GH Lee","year":"2016","unstructured":"Lee GH, Tan BH, Teo ECY, Lim SG, Dan YY, Wee A, Aw PPK, Zhu Y, Hibberd ML, Tan CK, Purdy MA (2016) Chronic infection with camelid hepatitis E virus in a liver transplant recipient who regularly consumes camel meat and milk. Gastroenterology 150(2):355\u2013357. https:\/\/doi.org\/10.1053\/j.gastro.2015.10.048","journal-title":"Gastroenterology"},{"key":"11342_CR33","doi-asserted-by":"publisher","first-page":"7207","DOI":"10.1128\/JVI.71.10.7207-7213.1997","volume":"71","author":"T-C Li","year":"1997","unstructured":"Li T-C, Yamakawa Y, Suzuki K, Tatsumi M, Razak MA, Uchida T, Takeda N, Miyamura T (1997) Expression and self-assembly of empty virus-like particles of hepatitis E virus. J Virol 71:7207\u20137213. https:\/\/doi.org\/10.1128\/JVI.71.10.7207-7213.1997","journal-title":"J Virol"},{"key":"11342_CR34","doi-asserted-by":"publisher","first-page":"370","DOI":"10.1016\/j.vaccine.2003.08.004","volume":"22","author":"T-C Li","year":"2004","unstructured":"Li T-C, Suzaki Y, Ami Y, Dhole TN, Miyamura T, Takeda N (2004) Protection of cynomolgus monkeys against HEV infection by oral administration of recombinant hepatitis E virus-like particles. Vaccine 22:370\u2013377. https:\/\/doi.org\/10.1016\/j.vaccine.2003.08.004","journal-title":"Vaccine"},{"key":"11342_CR35","doi-asserted-by":"publisher","first-page":"2893","DOI":"10.1016\/j.vaccine.2004.11.064","volume":"23","author":"SW Li","year":"2005","unstructured":"Li SW, Zhang J, Li YM, Ou SH, Huang GY, He ZQ, Ge SX, Xian YL, Pang SQ, Ng MH, Xia NS (2005a) A bacterially expressed particulate hepatitis E vaccine: antigenicity, immunogenicity and protectivity on primates. Vaccine 23:2893\u20132901. https:\/\/doi.org\/10.1016\/j.vaccine.2004.11.064","journal-title":"Vaccine"},{"key":"11342_CR36","doi-asserted-by":"publisher","first-page":"12999","DOI":"10.1128\/JVI.79.20.12999-13006.2005","volume":"79","author":"T-C Li","year":"2005","unstructured":"Li T-C, Takeda N, Miyamura T, Matsuura Y, Wang JCY, Engvall H, Hammar L, Xing L, Cheng RH (2005b) Essential elements of the capsid protein for self-assembly into empty virus-like particles of hepatitis E virus. J Virol 79:12999\u201313006. https:\/\/doi.org\/10.1128\/JVI.79.20.12999-13006.2005","journal-title":"J Virol"},{"key":"11342_CR37","doi-asserted-by":"publisher","first-page":"2830","DOI":"10.1099\/vir.0.034835-0","volume":"92","author":"T-C Li","year":"2011","unstructured":"Li T-C, Yoshimatsu K, Yasuda SP, Arikawa J, Koma T, Kataoka M, Ami Y, Suzaki Y, Le Mai TQ, Hoa NT, Yamashiro T, Hasebe F, Takeda N, Wakita T (2011) Characterisation of self-assembled virus-like particles of rat hepatitis E virus generated by recombinant baculoviruses. J Gen Virol 92:2830\u20132837. https:\/\/doi.org\/10.1099\/vir.0.034835-0","journal-title":"J Gen Virol"},{"key":"11342_CR38","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1016\/j.vetmic.2013.03.017","volume":"165","author":"W Li","year":"2013","unstructured":"Li W, Guan D, Su J, Takeda N, Wakita T, Li T-C, Ke CW (2013) High prevalence of rat hepatitis E virus in wild rats in China. Vet Microbiol 165:275\u2013280. https:\/\/doi.org\/10.1016\/j.vetmic.2013.03.017","journal-title":"Vet Microbiol"},{"key":"11342_CR39","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1016\/j.vetmic.2015.04.020","volume":"178","author":"T-C Li","year":"2015","unstructured":"Li T-C, Kataoka M, Takahashi K, Yoshizaki S, Kato T, Ishii K, Takeda N, Mishiro S, Wakita T (2015) Generation of hepatitis E virus-like particles of two new genotypes G5 and G6 and comparison of antigenic properties with those of known genotypes. Vet Microbiol 178:150\u2013157. https:\/\/doi.org\/10.1016\/j.vetmic.2015.04.020","journal-title":"Vet Microbiol"},{"issue":"6","key":"11342_CR40","doi-asserted-by":"publisher","first-page":"1104","DOI":"10.1016\/j.jhep.2016.07.013","volume":"65","author":"T-C Li","year":"2016","unstructured":"Li T-C, Zhou X, Yoshizaki S, Ami Y, Suzaki Y, Nakamura T, Takeda N, Wakita T (2016) Production of infectious dromedary camel hepatitis E virus by a reverse genetic system: potential for zoonotic infection. J Hepatol 65(6):1104\u20131111. https:\/\/doi.org\/10.1016\/j.jhep.2016.07.013","journal-title":"J Hepatol"},{"key":"11342_CR41","doi-asserted-by":"publisher","first-page":"e0122102","DOI":"10.1371\/journal.pone.0122102","volume":"10","author":"J Lin","year":"2015","unstructured":"Lin J, Karlsson M, Olofson AS, Belak S, Malmsten J, Dalin AM, Widen F, Norder H (2015) High prevalence of hepatitis E virus in Swedish moose--a phylogenetic characterisation and comparison of the virus from different regions. PLoS One 10:e0122102. https:\/\/doi.org\/10.1371\/journal.pone.0122102","journal-title":"PLoS One"},{"key":"11342_CR42","first-page":"1021","volume-title":"Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses","author":"XJ Meng","year":"2012","unstructured":"Meng XJ, Anderson DA, Arankalle VA, Emerson SU, Harrison TJ, Jameel S, Okamoto H (2012) Hepeviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses. Academic Press, London, pp 1021\u20131028"},{"key":"11342_CR43","doi-asserted-by":"publisher","unstructured":"Mulyanto, Suparyatmo JB, Andayani IGAS, Khalid Takahashi M, Ohnishi H, Jirintai S, Nagashima S, Nishizawa T, Okamoto H (2014) Marked genomic heterogeneity of rat hepatitis E virus strains in Indonesia demonstrated on a full-length genome analysis. Virus Res 179:102\u2013112. https:\/\/doi.org\/10.1016\/j.virusres.2013.10.029","DOI":"10.1016\/j.virusres.2013.10.029"},{"key":"11342_CR44","doi-asserted-by":"publisher","first-page":"979","DOI":"10.1007\/s00705-013-1912-3","volume":"159","author":"S Nagashima","year":"2014","unstructured":"Nagashima S, Takahashi M, Jirintai S, Tanggis Kobayashi T, Nishizawa T, Okamoto H (2014) The membrane on the surface of hepatitis E virus particles is derived from the intracellular membrane and contains trans-Golgi network protein 2. Arch Virol 159:979\u2013991. https:\/\/doi.org\/10.1007\/s00705-013-1912-3","journal-title":"Arch Virol"},{"key":"11342_CR45","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1016\/j.coviro.2014.12.006","volume":"10","author":"N Pavio","year":"2015","unstructured":"Pavio N, Meng XJ, Doceul V (2015) Zoonotic origin of hepatitis E. Curr Opin Virol 10:34\u201341. https:\/\/doi.org\/10.1016\/j.coviro.2014.12.006","journal-title":"Curr Opin Virol"},{"key":"11342_CR46","doi-asserted-by":"publisher","first-page":"117","DOI":"10.14218\/JCTH.2015.00009","volume":"3","author":"MT Perez-Gracia","year":"2015","unstructured":"Perez-Gracia MT, Garcia M, Suay B, Mateos-Lindemann ML (2015) Current knowledge on hepatitis E. J Clin Transl Hepatol 3:117\u2013126. https:\/\/doi.org\/10.14218\/JCTH.2015.00009","journal-title":"J Clin Transl Hepatol"},{"key":"11342_CR47","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1016\/j.jhep.2007.12.008","volume":"48","author":"RH Purcell","year":"2008","unstructured":"Purcell RH, Emerson SU (2008) Hepatitis E: an emerging awareness of an old disease. J Hepatol 48:494\u2013503. https:\/\/doi.org\/10.1016\/j.jhep.2007.12.008","journal-title":"J Hepatol"},{"key":"11342_CR48","doi-asserted-by":"publisher","first-page":"1369","DOI":"10.3201\/eid1808.111659","volume":"18","author":"VS Raj","year":"2012","unstructured":"Raj VS, Smits SL, Pas SD, Provacia LBV, Moorman-Roest H, Osterhaus ADME, Haagmans BL (2012) Novel hepatitis E virus in ferrets, the Netherlands. Emerg Infect Dis 18:1369\u20131370. https:\/\/doi.org\/10.3201\/eid1808.111659","journal-title":"Emerg Infect Dis"},{"key":"11342_CR49","doi-asserted-by":"publisher","first-page":"8011","DOI":"10.1128\/jvi.74.17.8011-8017.2000","volume":"74","author":"MA Riddell","year":"2000","unstructured":"Riddell MA, Li F, Anderson DA (2000) Identification of immunodominant and conformational epitopes in the capsid protein of hepatitis E virus by using monoclonal antibodies. J Virol 74:8011\u20138017. https:\/\/doi.org\/10.1128\/jvi.74.17.8011-8017.2000","journal-title":"J Virol"},{"key":"11342_CR50","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.vetmic.2017.07.001","volume":"208","author":"R Ryll","year":"2017","unstructured":"Ryll R, Bernstein S, Heuser E, Schlegel M, Dremsek P, Zumpe M, Wolf S, P\u00e9pin M, Bajomi D, M\u00fcller G, Heiberg A-C, Spahr C, Lang J, Groschup MH, Ansorge H, Freise J, Guenther S, Baert K, Ruiz-Fons F, Pikula J, Knap N, Tsakmakidis \u0399, Dovas C, Zanet S, Imholt C, Heckel G, Johne R, Ulrich RG (2017) Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries. Vet Microbiol 208:58\u201368. https:\/\/doi.org\/10.1016\/j.vetmic.2017.07.001","journal-title":"Vet Microbiol"},{"issue":"11","key":"11342_CR51","doi-asserted-by":"publisher","first-page":"2671","DOI":"10.1007\/s00705-019-04347-1","volume":"164","author":"R Ryll","year":"2019","unstructured":"Ryll R, Heckel G, Corman V, Drexler URG (2019) Genomic and spatial variability of a European common vole hepevirus. Arch Virol 164(11):2671\u20132682. https:\/\/doi.org\/10.1007\/s00705-019-04347-1","journal-title":"Arch Virol"},{"key":"11342_CR52","doi-asserted-by":"publisher","first-page":"1345","DOI":"10.1007\/s00705-011-0988-x","volume":"156","author":"Y Sato","year":"2011","unstructured":"Sato Y, Sato H, Naka K, Furuya S, Tsukiji H, Kitagawa K, Sonoda Y, Usui T, Sakamoto H, Yoshino S, Shimizu Y, Takahashi M, Nagashima S, Jirintai Nishizawa T, Okamoto H (2011) A nationwide survey of hepatitis E virus (HEV) infection in wild boars in Japan: identification of boar HEV strains of genotypes 3 and 4 and unrecognised genotypes. Arch Virol 156:1345\u20131358. https:\/\/doi.org\/10.1007\/s00705-011-0988-x","journal-title":"Arch Virol"},{"issue":"10","key":"11342_CR53","doi-asserted-by":"publisher","first-page":"267","DOI":"10.3390\/v8100267","volume":"8","author":"M Schemmerer","year":"2016","unstructured":"Schemmerer M, Apelt S, Trojnar E, Ulrich RG, Wenzel JJ, Johne R (2016) Enhanced replication of hepatitis E virus strain 47832c in an A549-derived subclonal cell line. Viruses. 8(10):267. https:\/\/doi.org\/10.3390\/v8100267","journal-title":"Viruses."},{"key":"11342_CR54","first-page":"649","volume":"18","author":"JA Schenk","year":"2004","unstructured":"Schenk JA, Matyssek F, Micheel B (2004) Interleukin 4 increases the antibody response against Rubisco in mice. In Vivo 18:649\u2013652","journal-title":"In Vivo"},{"key":"11342_CR55","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1016\/j.jbiotec.2011.12.025","volume":"158","author":"JA Schenk","year":"2012","unstructured":"Schenk JA, Fettke J, Lenz C, Albers K, Mallwitz F, Gajovic-Eichelmann N, Ehrentreich-F\u00f6rster E, Kusch E, Sellrie F (2012) Secretory leukocyte protease inhibitor (SLPI) might contaminate murine monoclonal antibodies after purification on protein G. J Biotechnol 158:34\u201335. https:\/\/doi.org\/10.1016\/j.jbiotec.2011.12.025","journal-title":"J Biotechnol"},{"key":"11342_CR56","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1186\/1743-422X-6-58","volume":"6","author":"A Schielke","year":"2009","unstructured":"Schielke A, Sachs K, Lierz M, Appel B, Jansen A, Johne R (2009) Detection of hepatitis E virus in wild boars of rural and urban regions in Germany and whole genome characterisation of an endemic strain. Virol J 6:58. https:\/\/doi.org\/10.1186\/1743-422X-6-58","journal-title":"Virol J"},{"key":"11342_CR57","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1002\/jmv.2031","volume":"65","author":"GG Schlauder","year":"2001","unstructured":"Schlauder GG, Mushahwar IK (2001) Genetic heterogeneity of hepatitis E virus. J Med Virol 65:282\u2013292. https:\/\/doi.org\/10.1002\/jmv.2031","journal-title":"J Med Virol"},{"key":"11342_CR58","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1186\/s13567-014-0121-8","volume":"45","author":"J Schlosser","year":"2014","unstructured":"Schlosser J, Eiden M, Vina-Rodriguez A, Fast C, Dremsek P, Lange E, Ulrich RG, Groschup MH (2014) Natural and experimental hepatitis E virus genotype 3-infection in European wild boar is transmissible to domestic pigs. Vet Res 45:121. https:\/\/doi.org\/10.1186\/s13567-014-0121-8","journal-title":"Vet Res"},{"key":"11342_CR59","doi-asserted-by":"publisher","first-page":"5548","DOI":"10.1128\/jvi.74.12.5548-5555.2000","volume":"74","author":"DJ Schofield","year":"2000","unstructured":"Schofield DJ, Glamann J, Emerson SU, Purcell RH (2000) Identification by phage display and characterisation of two neutralising chimpanzee monoclonal antibodies to the hepatitis E virus capsid protein. J Virol 74:5548\u20135555. https:\/\/doi.org\/10.1128\/jvi.74.12.5548-5555.2000","journal-title":"J Virol"},{"key":"11342_CR60","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/j.vaccine.2003.07.008","volume":"22","author":"DJ Schofield","year":"2003","unstructured":"Schofield DJ, Purcell RH, Nguyen HT, Emerson SU (2003) Monoclonal antibodies that neutralise HEV recognise an antigenic site at the carboxyterminus of an ORF2 protein vaccine. Vaccine 22:257\u2013267. https:\/\/doi.org\/10.1016\/j.vaccine.2003.07.008","journal-title":"Vaccine"},{"key":"11342_CR61","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/jvh.12024","volume":"20","author":"L Scobie","year":"2013","unstructured":"Scobie L, Dalton HR (2013) Hepatitis E: source and route of infection, clinical manifestations and new developments. J Viral Hepat 20:1\u201311. https:\/\/doi.org\/10.1111\/jvh.12024","journal-title":"J Viral Hepat"},{"key":"11342_CR62","doi-asserted-by":"publisher","first-page":"1677","DOI":"10.1292\/jvms.16-0200","volume":"78","author":"K Shimizu","year":"2016","unstructured":"Shimizu K, Hamaguchi S, Ngo CC, Li T-C, Ando S, Yoshimatsu K, Yasuda SP, Koma T, Isozumi R, Tsuda Y, Fujita H, Pham TT, Le MQ, Dang AD, Nguyen TQ, Yoshida L-M, Ariyoshi K, Arikawa J (2016) Serological evidence of infection with rodent-borne hepatitis E virus HEV-C1 or antigenically related virus in humans. J Vet Med Sci 78:1677\u20131681. https:\/\/doi.org\/10.1292\/jvms.16-0200","journal-title":"J Vet Med Sci"},{"issue":"1","key":"11342_CR63","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1007\/s00253-017-8622-9","volume":"102","author":"M Simanavicius","year":"2018","unstructured":"Simanavicius M, Tamosiunas PL, Petraityte-Burneikiene R, Johne R, Ulrich RG, Zvirbliene A, Kucinskaite-Kodze I (2018) Generation in yeast and antigenic characterization of hepatitis E virus capsid protein virus-like particles. Appl Microbiol Biotechnol 102(1):185\u2013198. https:\/\/doi.org\/10.1007\/s00253-017-8622-9","journal-title":"Appl Microbiol Biotechnol"},{"key":"11342_CR64","doi-asserted-by":"publisher","first-page":"2223","DOI":"10.1099\/vir.0.068429-0","volume":"95","author":"DB Smith","year":"2014","unstructured":"Smith DB, Simmonds P, Jameel S, Emerson SU, Harrison TJ, Meng X-J, Okamoto H, Van der Poel WHM, Purdy MA (2014) Consensus proposals for classification of the family Hepeviridae. J Gen Virol 95:2223\u20132232. https:\/\/doi.org\/10.1099\/vir.0.068429-0","journal-title":"J Gen Virol"},{"key":"11342_CR65","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1099\/jgv.0.000393","volume":"97","author":"DB Smith","year":"2016","unstructured":"Smith DB, Simmonds P, Izopet J, Oliveira-Filho EF, Ulrich RG, Johne R, Koenig M, Jameel S, Harrison TJ, Meng X-J, Okamoto H, Van der Poel WHM, Purdy MA (2016) Proposed reference sequences for hepatitis E virus subtypes. J Gen Virol 97:537\u2013542. https:\/\/doi.org\/10.1099\/jgv.0.000393","journal-title":"J Gen Virol"},{"issue":"7","key":"11342_CR66","doi-asserted-by":"publisher","first-page":"692","DOI":"10.1099\/jgv.0.001435","volume":"101","author":"DB Smith","year":"2020","unstructured":"Smith DB, Izopet J, Nicot F, Simmonds P, Jameel S, Meng XJ, Norder H, Okamoto H, van der Poel WHM, Reuter G, Purdy MA (2020) Update: proposed reference sequences for subtypes of hepatitis E virus (species Orthohepevirus A). J Gen Virol 101(7):692\u2013698. https:\/\/doi.org\/10.1099\/jgv.0.001435","journal-title":"J Gen Virol"},{"issue":"12","key":"11342_CR67","doi-asserted-by":"publisher","first-page":"2241","DOI":"10.3201\/eid2412.180937","volume":"24","author":"S Sridhar","year":"2018","unstructured":"Sridhar S, Yip C, Wu S, Cai J, Zhang A, Leung K, Yuen K (2018) Rat hepatitis E virus as cause of persistent hepatitis after liver pransplant. Emerg Infect Dis 24(12):2241\u20132250. https:\/\/doi.org\/10.3201\/eid2412.180937","journal-title":"Emerg Infect Dis"},{"issue":"1","key":"11342_CR68","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1002\/hep.31138","volume":"73","author":"S Sridhar","year":"2021","unstructured":"Sridhar S, Yip CCY, Wu S, Chew NFS, Leung KH, Chan JFW, Zhao PS, Chan WM, Poon RWS, Tsoi HW, Cai JP (2021) Transmission of rat hepatitis E virus infection to humans in Hong Kong: A clinical and epidemiological analysis. Hepatology 73(1):10\u201322. https:\/\/doi.org\/10.1002\/hep.31138","journal-title":"Hepatology"},{"issue":"2","key":"11342_CR69","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1016\/j.jbiotec.2012.08.020","volume":"164","author":"M Stech","year":"2012","unstructured":"Stech M, Merk H, Schenk JA, St\u00f6cklein WFM, W\u00fcstenhagen DA, Micheel B, Duschl C, Bier FF, Kubick S (2012) Production of functional antibody fragments in a vesicle-based eukaryotic cell-free translation system. J Biotechnol 164(2):220\u2013231. https:\/\/doi.org\/10.1016\/j.jbiotec.2012.08.020","journal-title":"J Biotechnol"},{"key":"11342_CR70","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1007\/s00705-008-0045-6","volume":"153","author":"M Takahashi","year":"2008","unstructured":"Takahashi M, Hoshino Y, Tanaka T, Takahashi H, Nishizawa T, Okamoto H (2008a) Production of monoclonal antibodies against hepatitis E virus capsid protein and evaluation of their neutralising activity in a cell culture system. Arch Virol 153:657\u2013666. https:\/\/doi.org\/10.1007\/s00705-008-0045-6","journal-title":"Arch Virol"},{"key":"11342_CR71","doi-asserted-by":"publisher","first-page":"1703","DOI":"10.1007\/s00705-008-0179-6","volume":"153","author":"M Takahashi","year":"2008","unstructured":"Takahashi M, Yamada K, Hoshino Y, Takahashi H, Ichiyama K, Tanaka T, Okamoto H (2008b) Monoclonal antibodies raised against the ORF3 protein of hepatitis E virus (HEV) can capture HEV particles in culture supernatant and serum but not those in feces. Arch Virol 153:1703\u20131713. https:\/\/doi.org\/10.1007\/s00705-008-0179-6","journal-title":"Arch Virol"},{"key":"11342_CR72","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1016\/j.virusres.2013.12.014","volume":"180","author":"M Takahashi","year":"2014","unstructured":"Takahashi M, Nishizawa T, Nagashima S, Jirintai S, Kawakami M, Sonoda Y, Suzuki T, Yamamoto S, Shigemoto K, Ashida K, Sato Y, Okamoto H (2014) Molecular characterisation of a novel hepatitis E virus (HEV) strain obtained from a wild boar in Japan that is highly divergent from the previously recognised HEV strains. Virus Res 180:59\u201369. https:\/\/doi.org\/10.1016\/j.virusres.2013.12.014","journal-title":"Virus Res"},{"issue":"9","key":"11342_CR73","doi-asserted-by":"publisher","first-page":"e0163670","DOI":"10.1371\/journal.pone.0163670","volume":"11","author":"L Thoring","year":"2016","unstructured":"Thoring L, W\u00fcstenhagen DA, Borowiak M, Stech M, Sonnabend A, Kubick S (2016) Cell-free systems based on CHO cell lysates: optimisation strategies, synthesis of \"difficult-to-express\" proteins and future perspectives. PLoS One 11(9):e0163670. https:\/\/doi.org\/10.1371\/journal.pone.0163670","journal-title":"PLoS One"},{"key":"11342_CR74","doi-asserted-by":"publisher","unstructured":"Thoring L, Zemella A, W\u00fcstenhagen D, Kubick S (2019) Accelerating the production of druggable targets: Eukaryotic cell-free systems come into focus. Methods Protoc 2(2). https:\/\/doi.org\/10.3390\/mps2020030","DOI":"10.3390\/mps2020030"},{"key":"11342_CR75","doi-asserted-by":"publisher","first-page":"1834","DOI":"10.1016\/S0264-410X(97)00145-X","volume":"15","author":"S Tsarev","year":"1997","unstructured":"Tsarev S (1997) Recombinant vaccine against hepatitis E: dose response and protection against heterologous challenge. Vaccine 15:1834\u20131838. https:\/\/doi.org\/10.1016\/S0264-410X(97)00145-X","journal-title":"Vaccine"},{"issue":"9","key":"11342_CR76","doi-asserted-by":"publisher","first-page":"2417","DOI":"10.1007\/s00705-019-04306-w","volume":"164","author":"PJ Walker","year":"2019","unstructured":"Walker PJ, Siddell SG, Lefkowitz EJ, Mushegian AR, Dempsey DM, Dutilh BE, Harrach B, Harrison RL, Hendrickson RC, Junglen S, Knowles NJ (2019) Changes to virus taxonomy and the International Code of Virus Classification and Nomenclature ratified by the International Committee on Taxonomy of Viruses (2019). Arch Virol 164(9):2417\u20132429. https:\/\/doi.org\/10.1007\/s00705-019-04306-w","journal-title":"Arch Virol"},{"key":"11342_CR77","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1016\/j.virusres.2013.05.013","volume":"176","author":"S Wang","year":"2013","unstructured":"Wang S, Cheng X, Dai X, Dong C, Xu M, Liang J, Dong M, Purdy MA, Meng J (2013) Rabbit and human hepatitis E virus strains belong to a single serotype. Virus Res 176:101\u2013106. https:\/\/doi.org\/10.1016\/j.virusres.2013.05.013","journal-title":"Virus Res"},{"issue":"10","key":"11342_CR78","doi-asserted-by":"publisher","first-page":"5491","DOI":"10.1128\/JVI.00107-15","volume":"89","author":"X Wang","year":"2015","unstructured":"Wang X, Zhao Q, Dang L, Sun Y, Gao J, Liu B, Syed SF, Tao H, Zhang G, Luo J, Zhou EM (2015) Characterization of two novel linear B-cell epitopes in the capsid protein of avian hepatitis E virus (HEV) that are common to avian, swine, and human HEVs. J Virol 89(10):5491\u20135501. https:\/\/doi.org\/10.1128\/JVI.00107-15","journal-title":"J Virol"},{"issue":"4","key":"11342_CR79","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1007\/s12250-017-3973-z","volume":"32","author":"B Wang","year":"2017","unstructured":"Wang B, Cai CL, Li B, Zhang W, Zhu Y, Chen WH, Zhuo F, Shi ZL, Yang XL (2017) Detection and characterisation of three zoonotic viruses in wild rodents and shrews from Shenzhen city, China. Virol Sin 32(4):290\u2013297. https:\/\/doi.org\/10.1007\/s12250-017-3973-z","journal-title":"Virol Sin"},{"key":"11342_CR80","doi-asserted-by":"publisher","first-page":"1575","DOI":"10.1007\/s00705-013-1646-2","volume":"158","author":"S Wolf","year":"2013","unstructured":"Wolf S, Reetz J, Johne R, Heiberg A-C, Petri S, Kanig H, Ulrich RG (2013) The simultaneous occurrence of human norovirus and hepatitis E virus in a Norway rat (Rattus norvegicus). Arch Virol 158:1575\u20131578. https:\/\/doi.org\/10.1007\/s00705-013-1646-2","journal-title":"Arch Virol"},{"key":"11342_CR81","doi-asserted-by":"publisher","first-page":"1044","DOI":"10.3201\/eid2006.140140","volume":"20","author":"PCY Woo","year":"2014","unstructured":"Woo PCY, Lau SKP, Teng JLL, Tsang AKL, Joseph M, Wong EYM, Tang Y, Sivakumar S, Xie J, Bai R, Wernery R, Wernery U, Yuen K-Y (2014) New hepatitis E virus genotype in camels, the Middle East. Emerg Infect Dis 20:1044\u20131048. https:\/\/doi.org\/10.3201\/eid2006.140140","journal-title":"Emerg Infect Dis"},{"key":"11342_CR82","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1006\/viro.1999.0005","volume":"265","author":"L Xing","year":"1999","unstructured":"Xing L, Kato K, Li T, Takeda N, Miyamura T, Hammar L, Cheng RH (1999) Recombinant hepatitis E capsid protein self-assembles into a dual-domain T = 1 particle presenting native virus epitopes. Virology 265:35\u201345. https:\/\/doi.org\/10.1006\/viro.1999.0005","journal-title":"Virology"},{"key":"11342_CR83","doi-asserted-by":"publisher","first-page":"12986","DOI":"10.1073\/pnas.0903699106","volume":"106","author":"T Yamashita","year":"2009","unstructured":"Yamashita T, Mori Y, Miyazaki N, Cheng RH, Yoshimura M, Unno H, Shima R, Moriishi K, Tsukihara T, Li TC, Takeda N, Miyamura T, Matsuura Y (2009) Biological and immunological characteristics of hepatitis E virus-like particles based on the crystal structure. Proc Natl Acad Sci U S A 106:12986\u201312991. https:\/\/doi.org\/10.1073\/pnas.0903699106","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"18","key":"11342_CR84","doi-asserted-by":"publisher","first-page":"4773","DOI":"10.1073\/pnas.1721345115","volume":"115","author":"X Yin","year":"2018","unstructured":"Yin X, Ying D, Lhomme S, Tang Z, Walker CM, Xia N, Zheng Z, Feng Z (2018) Origin, antigenicity, and function of a secreted form of ORF2 in hepatitis E virus infection. Proc Natl Acad Sci U S A 115(18):4773\u20134778. https:\/\/doi.org\/10.1073\/pnas.1721345115","journal-title":"Proc Natl Acad Sci U S A"},{"key":"11342_CR85","doi-asserted-by":"publisher","first-page":"9045","DOI":"10.1128\/JVI.71.12.9045-9053.1997","volume":"71","author":"M Zafrullah","year":"1997","unstructured":"Zafrullah M, Ozdener MH, Panda SK, Jameel S (1997) The ORF3 protein of hepatitis E virus is a phosphoprotein that associates with the cytoskeleton. J Virol 71:9045\u20139053. https:\/\/doi.org\/10.1128\/JVI.71.12.9045-9053.1997","journal-title":"J Virol"},{"key":"11342_CR86","doi-asserted-by":"publisher","first-page":"2881","DOI":"10.1016\/j.vaccine.2004.11.065","volume":"23","author":"J Zhang","year":"2005","unstructured":"Zhang J, Gu Y, Ge SX, Li SW, He ZQ, Huang GY, Zhuang H, Ng MH, Xia NS (2005) Analysis of hepatitis E virus neutralisation sites using monoclonal antibodies directed against a virus capsid protein. Vaccine 23:2881\u20132892. https:\/\/doi.org\/10.1016\/j.vaccine.2004.11.065","journal-title":"Vaccine"},{"key":"11342_CR87","doi-asserted-by":"publisher","first-page":"19910","DOI":"10.1074\/jbc.M115.649764","volume":"290","author":"M Zhao","year":"2015","unstructured":"Zhao M, Li X-J, Tang Z-M, Yang F, Wang S-L, Cai W, Zhang K, Xia N-S, Zheng Z-Z (2015) A comprehensive study of neutralising antigenic sites on the hepatitis E virus (HEV) capsid by constructing, clustering, and characterising a tool box. J Biol Chem 290:19910\u201319922. https:\/\/doi.org\/10.1074\/jbc.M115.649764","journal-title":"J Biol Chem"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-021-11342-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00253-021-11342-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-021-11342-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,26]],"date-time":"2021-06-26T04:43:37Z","timestamp":1624682617000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00253-021-11342-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6]]},"references-count":87,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,6]]}},"alternative-id":["11342"],"URL":"https:\/\/doi.org\/10.1007\/s00253-021-11342-7","relation":{},"ISSN":["0175-7598","1432-0614"],"issn-type":[{"value":"0175-7598","type":"print"},{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6]]},"assertion":[{"value":"13 December 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 April 2021","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 May 2021","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 June 2021","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The mice were housed and handled in unity with good animal practice as defined by the Federation of European Laboratory Animal Science Associations (FELASA). All animal protocols were approved by local authorities (Landesamt f\u00fcr Umwelt, Gesundheit und Verbraucherschutz (LUGV) Brandenburg, Germany; Permit-Number: 2347-A-34-1-2020).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"The authors declare that they have no conflict of interest.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}