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Multiple virulence factors have been implicated in the high prevalence rate of <jats:italic>A. baumannii<\/jats:italic> in hospitalized and immunocompromised patients. Moreover, improper use of antibiotics has led to the emergence of extensive drug-resistant strains that urgently require alternative strategies to control this superbug. Unfortunately, the availability of a licensed vaccine against <jats:italic>A. baumannii<\/jats:italic> infections is still challenged by the vast diversity among <jats:italic>A. baumannii<\/jats:italic> strains. Here, we report the development of a novel pentavalent vaccine candidate composed of two recombinant proteins (Wza and YiaD) and a pool of capsular polysaccharides isolated from 3 clinical isolates. We tested this new vaccine <jats:italic>in vivo<\/jats:italic> in a mouse model of peritonitis against the standard strain ATCC 19606 in addition to 3 clinical isolates of <jats:italic>A. baumannii<\/jats:italic>. Immunization with this vaccine completely protected the challenged mice with 100% survival rate in the case of all the tested bacteria. Further clinical studies are urgently needed to evaluate the efficacy and safety of this proprietary vaccine to protect patients from <jats:italic>A. baumannii<\/jats:italic> lethal infections.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Key points<\/jats:title>\n                <jats:p><jats:italic>\u2022 Recombinant proteins pool (Wza and YiaD) immunization led to a synergistic immune response.<\/jats:italic><\/jats:p>\n                <jats:p><jats:italic>\u2022 Capsular polysaccharides pool induced up to 90% protection of tested clinical isolates.<\/jats:italic><\/jats:p>\n                <jats:p><jats:italic>\u2022 The pentavalent pool showed superiority with 100% survival of immunized mice.<\/jats:italic><\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s00253-022-12231-3","type":"journal-article","created":{"date-parts":[[2022,11,19]],"date-time":"2022-11-19T06:02:59Z","timestamp":1668837779000},"page":"8151-8167","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A novel pentavalent vaccine candidate completely protects against Acinetobacter baumannii in a mouse model of peritonitis"],"prefix":"10.1007","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9008-1304","authenticated-orcid":false,"given":"Yomna A.","family":"Hagag","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4456-6001","authenticated-orcid":false,"given":"Heba Shehta","family":"Said","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8548-6649","authenticated-orcid":false,"given":"Hany I.","family":"Kenawy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8336-9847","authenticated-orcid":false,"given":"Ramadan","family":"Hassan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,11,19]]},"reference":[{"key":"12231_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jmgm.2018.04.020","volume":"83","author":"S Ahmad","year":"2018","unstructured":"Ahmad S, Azam SS (2018) A novel approach of virulome based reverse vaccinology for exploring and validating peptide-based vaccine candidates against the most troublesome nosocomial pathogen: Acinetobacter baumannii. 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