{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:52:49Z","timestamp":1790801569570,"version":"4.1.0"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"24","license":[{"start":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T00:00:00Z","timestamp":1668556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T00:00:00Z","timestamp":1668556800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1107707"],"award-info":[{"award-number":["OPP1107707"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014825","name":"Biotechnology Industry Research Assistance Council","doi-asserted-by":"publisher","award":["BIRAC\/GCI\/0067\/02\/13-RTTC"],"award-info":[{"award-number":["BIRAC\/GCI\/0067\/02\/13-RTTC"]}],"id":[{"id":"10.13039\/501100014825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:sec>\n                <jats:title>Abstract\n<\/jats:title>\n                <jats:p>Bacterial pathogens are fostered in and transmitted through wastewater. Hence, monitoring their impact on sanitation and hygiene is imperative. As part of the monitoring process, culture-based methodologies are primarily used, which centre on the use of selective and differential media. Media available today are, at best, difficult to formulate and, at worst, prohibitively expensive. To address this lacuna, the study proposes a selective and differential medium for <jats:italic>Klebsiella<\/jats:italic> spp. <jats:italic>Klebsiella<\/jats:italic> blue agar (KBA) is completely selective against selected gram-positive bacteria (<jats:italic>Bacillus<\/jats:italic> spp., <jats:italic>Staphylococcus aureus<\/jats:italic>) and a few gram-negative bacteria (<jats:italic>Acinetobacter baumanii<\/jats:italic>, <jats:italic>Serratia marcescens<\/jats:italic>)<jats:italic>.<\/jats:italic> On the other hand, it supports the growth of the chosen members of the <jats:italic>Klebsiella pneumoniae<\/jats:italic> species-complex with a characteristic green colouration. Methylene blue, tryptophan, and bile salt make up the selective components of KBA. Moreover, methylene blue, 0.6% NaCl, and glycerol render it differential. KBA was more selective than HiCrome\u2122 Klebsiella Selective Agar Base (KSA) in replica plating experiments. KBA promoted only 157 CFUs against 209 CFUs in KSA when stamped with 253 CFUs grown on LB. The colonies so isolated were predominantly <jats:italic>Klebsiella<\/jats:italic> spp., on identification through colony polymerase chain reaction. Moreover, the differential nature of KBA distinguished <jats:italic>Klebsiella aerogenes<\/jats:italic> from other species. On the contrary, KSA lodged colonies indistinguishable from each other and <jats:italic>Klebsiella<\/jats:italic> spp. Due to its ease of formulation, high selectivity, differential nature, and cost-effective composition, KBA is a viable option for the routine culture of <jats:italic>Klebsiella<\/jats:italic> spp. in environmental and clinical settings.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Key points<\/jats:title>\n                <jats:p>\u2022 <jats:italic>Formulated a novel selective and differential media for Klebsiella spp., named Klebsiella Blue agar<\/jats:italic><\/jats:p>\n                <jats:p>\u2022 <jats:italic>Facile formulation methodology<\/jats:italic><\/jats:p>\n                <jats:p>\u2022 <jats:italic>Can be employed to isolate Klebsiella spp. from complex sources such as wastewater<\/jats:italic><\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s00253-022-12270-w","type":"journal-article","created":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T03:04:30Z","timestamp":1668567870000},"page":"8273-8284","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A novel and improved selective media for the isolation and enumeration of Klebsiella species"],"prefix":"10.1007","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1560-5267","authenticated-orcid":false,"given":"Megha","family":"Prasad","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3661-156X","authenticated-orcid":false,"given":"Sindhu K.","family":"Shetty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4944-8805","authenticated-orcid":false,"given":"Bipin G.","family":"Nair","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2012-0734","authenticated-orcid":false,"given":"Sanjay","family":"Pal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4519-0851","authenticated-orcid":false,"given":"Ajith","family":"Madhavan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,11,16]]},"reference":[{"key":"12270_CR1","doi-asserted-by":"publisher","unstructured":"Babu R, Kumar A, Karim S, Warrier S, Nair SG, Singh SK, Biswas R (2016) Faecal carriage rate of extended-spectrum \u03b2-lactamase-producing Enterobacteriaceae in hospitalised patients and healthy asymptomatic individuals coming for health check-up. J Glob Antimicrob Resist 6:150\u2013153. https:\/\/doi.org\/10.1016\/J.JGAR.2016.05.007","DOI":"10.1016\/J.JGAR.2016.05.007"},{"key":"12270_CR2","doi-asserted-by":"publisher","unstructured":"Bonardi S, Pitino R (2019) Carbapenemase-producing bacteria in food-producing animals, wildlife and environment: a challenge for human health.\u00a0Ital J Food Saf\u00a08(2):7956. https:\/\/doi.org\/10.4081\/ijfs.2019.7956","DOI":"10.4081\/ijfs.2019.7956"},{"key":"12270_CR3","doi-asserted-by":"publisher","first-page":"153926","DOI":"10.1016\/J.SCITOTENV.2022.153926","volume":"825","author":"FR Charles","year":"2022","unstructured":"Charles FR, Lim JX, Chen H, Goh SG, He Y, Gin KYH (2022) Prevalence and characterization of antibiotic resistant bacteria in raw community sewage from diverse urban communities. Sci Total Environ 825:153926. https:\/\/doi.org\/10.1016\/J.SCITOTENV.2022.153926","journal-title":"Sci Total Environ"},{"key":"12270_CR4","doi-asserted-by":"publisher","first-page":"E1610","DOI":"10.1073\/PNAS.1401941111\/-\/DCSUPPLEMENTAL","volume":"111","author":"CM Cremers","year":"2014","unstructured":"Cremers CM, Knoefler D, Vitvitsky V, Banerjee R, Jakob U (2014) Bile salts act as effective protein-unfolding agents and instigators of disulfide stress in vivo. Proc Natl Acad Sci U S A 111:E1610. https:\/\/doi.org\/10.1073\/PNAS.1401941111\/-\/DCSUPPLEMENTAL","journal-title":"Proc Natl Acad Sci U S A"},{"key":"12270_CR5","doi-asserted-by":"publisher","first-page":"139631","DOI":"10.1016\/J.SCITOTENV.2020.139631","volume":"736","author":"CG Daughton","year":"2020","unstructured":"Daughton CG (2020) Wastewater surveillance for population-wide Covid-19: the present and future. Sci Total Environ 736:139631. https:\/\/doi.org\/10.1016\/J.SCITOTENV.2020.139631","journal-title":"Sci Total Environ"},{"key":"12270_CR6","doi-asserted-by":"publisher","unstructured":"Dinkelacker AG, Vogt S, Oberhettinger P, Mauder N, Rau J, Kostrzewa M, Rossen J, Autenrieth IB, Peter S, Liese J (2018) Typing and species identification of clinical\u00a0Klebsiella isolates by fourier transform infrared spectroscopy and matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 56(11):e00843-18. https:\/\/doi.org\/10.1128\/JCM.00843-18","DOI":"10.1128\/JCM.00843-18"},{"key":"12270_CR7","doi-asserted-by":"publisher","unstructured":"Divakaran SJ, Philip JS, Chereddy P, Nori SRC, Ganesh AJ, John J, Nelson-Sathi S (2019) Insights into the bacterial profiles and resistome structures following the severe 2018 flood in Kerala, South India. Microorganisms 7, Page 474 7:474. https:\/\/doi.org\/10.3390\/MICROORGANISMS7100474","DOI":"10.3390\/MICROORGANISMS7100474"},{"key":"12270_CR8","doi-asserted-by":"publisher","first-page":"103998","DOI":"10.1016\/J.EBIOM.2022.103998","volume":"79","author":"N Dong","year":"2022","unstructured":"Dong N, Yang X, Chan EWC, Zhang R, Chen S (2022) Klebsiella species: taxonomy, hypervirulence and multidrug resistance. EBioMedicine 79:103998. https:\/\/doi.org\/10.1016\/J.EBIOM.2022.103998","journal-title":"EBioMedicine"},{"key":"12270_CR9","doi-asserted-by":"crossref","unstructured":"Dworkin M, Falkow S, Rosenberg E, Schleifer K-H, Stackebrandt E (eds) (2006) The prokaryotes, 3rd edn. Springer New York, New York, NY","DOI":"10.1007\/0-387-30745-1"},{"key":"12270_CR10","doi-asserted-by":"publisher","unstructured":"Fonseca EL, Ramos N da V, Andrade BGN, Morais LLCS, Marin MFA, Vicente ACP (2017) A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine. Diagn Microbiol Infect Dis 87:315\u2013317. https:\/\/doi.org\/10.1016\/j.diagmicrobio.2017.01.005","DOI":"10.1016\/j.diagmicrobio.2017.01.005"},{"key":"12270_CR11","doi-asserted-by":"publisher","unstructured":"Galarde-L\u00f3pez M, Velazquez-Meza ME, Bobadilla-Del-Valle M, Carrillo-Quiroz BA, Cornejo-Ju\u00e1rez P, Ponce-de-Le\u00f3n A, Sasso\u00e9-Gonz\u00e1lez A, Alpuche-Aranda CM (2022) Surveillance of antimicrobial resistance in hospital wastewater: identification of carbapenemase-producing Klebsiella spp. Antibiotics (Basel, Switzerland) 11(3):288. https:\/\/doi.org\/10.3390\/antibiotics11030288","DOI":"10.3390\/antibiotics11030288"},{"key":"12270_CR12","doi-asserted-by":"publisher","unstructured":"Glupczynski Y, Berhin C, Bauraing C, Bogaerts P (2007) Evaluation of a new selective chromogenic agar medium for detection of extended-spectrum \u03b2-lactamase-producing\u00a0Enterobacteriaceae. J Clin Microbiol 45:501\u2013505.\u00a0https:\/\/doi.org\/10.1128\/JCM.02221-06","DOI":"10.1128\/JCM.02221-06"},{"key":"12270_CR13","doi-asserted-by":"publisher","unstructured":"Gomi R, Matsuda T, Yamamoto M, Chou PH, Tanaka M, Ichiyama S, Yoneda M, Matsumura Y (2018) Characteristics of carbapenemase-producing Enterobacteriaceae in wastewater revealed by genomic analysis. Antimicrob Agents Chemother 62(5):e02501-17. https:\/\/doi.org\/10.1128\/AAC.02501-17","DOI":"10.1128\/AAC.02501-17"},{"key":"12270_CR14","doi-asserted-by":"publisher","unstructured":"Grimont PAD, Grimont F (2015) Klebsiella. In: Trujillo ME, Dedysh P, DeVos B, Hedlund B, Kampfer P, Rainey FA et al (eds) Bergey\u2019s manual of systematics in archaea and bacteria. John Wiley & Sons, Inc., Hoboken, NJ. https:\/\/doi.org\/10.1002\/9781118960608.gbm01150","DOI":"10.1002\/9781118960608.gbm01150"},{"key":"12270_CR15","doi-asserted-by":"publisher","first-page":"E3574","DOI":"10.1073\/pnas.1501049112","volume":"112","author":"KE Holt","year":"2015","unstructured":"Holt KE, Wertheim H, Zadoks RN, Baker S, Whitehouse CA, Dance D, Jenney A, Connor TR, Hsu LY, Severin J, Brisse S, Cao H, Wilksch J, Gorrie C, Schultz MB, Edwards DJ, van Nguyen K, Nguyen TV, Dao TT, Mensink M, le Minh V, Nhu NTK, Schultsz C, Kuntaman K, Newton PN, Moore CE, Strugnell RA, Thomson NR (2015) Genomic analysis of diversity, population structure, virulence, and antimicrobial resistance in Klebsiella pneumoniae, an urgent threat to public health. Proc Natl Acad Sci USA 112:E3574-3581. https:\/\/doi.org\/10.1073\/pnas.1501049112","journal-title":"Proc Natl Acad Sci USA"},{"key":"12270_CR16","doi-asserted-by":"publisher","first-page":"348","DOI":"10.1136\/JCLINPATH-2012-201234","volume":"66","author":"M Hornsey","year":"2013","unstructured":"Hornsey M, Phee L, Woodford N, Turton J, Meunier D, Thomas C, Wareham DW (2013) Evaluation of three selective chromogenic media, CHROMagar ESBL, CHROMagar CTX-M and CHROMagar KPC, for the detection of Klebsiella pneumoniae producing OXA-48 carbapenemase. J Clin Pathol 66:348\u2013350. https:\/\/doi.org\/10.1136\/JCLINPATH-2012-201234","journal-title":"J Clin Pathol"},{"key":"12270_CR17","unstructured":"IS 3025 (Part 11) (1983, Reaffirmed 2002): Method of Sampling and Test (Physical and Chemical) for Water and Wastewater, Part 11: pH Value (First Revision). ICS13.060.50"},{"key":"12270_CR18","unstructured":"IS 3025-16 (1984) Methods of sampling and test (physical and chemical) for water and wastewater, Part 16: filterable residue (total dissolved solids) [CHD 32: Environmental Protection and Waste Management]"},{"key":"12270_CR19","unstructured":"IS 3025-38 (1989) Methods of sampling and test (physical and chemical) for water and wastewater, part 38: dissolved oxygen [CHD 32: Environmental Protection and WasteManagement]"},{"key":"12270_CR20","doi-asserted-by":"publisher","first-page":"990","DOI":"10.1038\/NATURE06536","volume":"451","author":"KE Jones","year":"2008","unstructured":"Jones KE, Patel NG, Levy MA, Storeygard A, Balk D, Gittleman JL, Daszak P (2008) Global trends in emerging infectious diseases. Nature 451:990. https:\/\/doi.org\/10.1038\/NATURE06536","journal-title":"Nature"},{"key":"12270_CR21","unstructured":"Kisan M, Sangathan S, Nehru J, Pitroda SG\u00a0(1993) I.S.: 3025 (Part 44), methods of sampling and test (physical and chemical) for water and waste water, part 44 Biological Oxygen Demand (BOD)"},{"key":"12270_CR22","first-page":"406","volume":"31","author":"A Kumar","year":"2018","unstructured":"Kumar A, Biswas L, Omgy N, Mohan K, Vinod V, Sajeev A, Nair P, Singh S, Biswas R (2018) Colistin resistance due to insertional inactivation of the mgrB in Klebsiella pneumoniae of clinical origin: First report from India: Resistencia a colistina debido a inactivaci\u00f3n insercional del gen mgrB en aislados cl\u00ednicos de Klebsiella pneumoniae: Primera notificaci\u00f3n en India. Rev Esp Quimioter 31:406","journal-title":"Rev Esp Quimioter"},{"key":"12270_CR23","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1016\/J.BIOTECHADV.2017.10.004","volume":"36","author":"V Kumar","year":"2018","unstructured":"Kumar V, Park S (2018) Potential and limitations of Klebsiella pneumoniae as a microbial cell factory utilizing glycerol as the carbon source. Biotechnol Adv 36:150\u2013167. https:\/\/doi.org\/10.1016\/J.BIOTECHADV.2017.10.004","journal-title":"Biotechnol Adv"},{"key":"12270_CR24","doi-asserted-by":"publisher","first-page":"4","DOI":"10.3389\/FCIMB.2018.00004\/BIBTEX","volume":"8","author":"RM Martin","year":"2018","unstructured":"Martin RM, Bachman MA (2018) Colonization, infection, and the accessory genome of Klebsiella pneumoniae. Front Cell Infect Microbiol 8:4. https:\/\/doi.org\/10.3389\/FCIMB.2018.00004\/BIBTEX","journal-title":"Front Cell Infect Microbiol"},{"key":"12270_CR25","doi-asserted-by":"publisher","first-page":"1656","DOI":"10.1128\/AAC.04292-14\/SUPPL_FILE\/ZAC003153789SO1.PDF","volume":"59","author":"AJ Mathers","year":"2015","unstructured":"Mathers AJ, Stoesser N, Sheppard AE, Pankhurst L, Giess A, Yeh AJ, Didelot X, Turner SD, Sebra R, Kasarskis A, Peto T, Crook D, Sifri CD (2015) Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae at a single institution: insights into endemicity from whole-genome sequencing. Antimicrob Agents Chemother 59:1656\u20131663. https:\/\/doi.org\/10.1128\/AAC.04292-14\/SUPPL_FILE\/ZAC003153789SO1.PDF","journal-title":"Antimicrob Agents Chemother"},{"key":"12270_CR26","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1756-0500-7-215\/TABLES\/5","volume":"7","author":"F Moges","year":"2014","unstructured":"Moges F, Endris M, Belyhun Y, Worku W (2014) Isolation and characterization of multiple drug resistance bacterial pathogens from wastewater in hospital and non-hospital environments, Northwest Ethiopia. BMC Res Notes 7:1\u20136. https:\/\/doi.org\/10.1186\/1756-0500-7-215\/TABLES\/5","journal-title":"BMC Res Notes"},{"key":"12270_CR27","doi-asserted-by":"publisher","unstructured":"Perez-Palacios P, Delgado-Valverde M, Gual-de-Torrella A, Oteo-Iglesias J, Pascual \u00c1, Fern\u00e1ndez-Cuenca F (2021a) Co-transfer of plasmid-encoded bla carbapenemases genes and mercury\u00a0resistance operon in high-risk clones of Klebsiella pneumoniae. Appl Microbiol Biotechnol 105:24 105:9231\u20139242. https:\/\/doi.org\/10.1007\/S00253-021-11684-2","DOI":"10.1007\/S00253-021-11684-2"},{"key":"12270_CR28","doi-asserted-by":"publisher","first-page":"9231","DOI":"10.1007\/S00253-021-11684-2\/FIGURES\/3","volume":"105","author":"P Perez-Palacios","year":"2021","unstructured":"Perez-Palacios P, Delgado-Valverde M, Gual-de-Torrella A, Oteo-Iglesias J, Pascual \u00c1, Fern\u00e1ndez-Cuenca F (2021) Co-transfer of plasmid-encoded bla carbapenemases genes and mercury resistance operon in high-risk clones of Klebsiella pneumoniae. Appl Microbiol Biotechnol 105:9231\u20139242. https:\/\/doi.org\/10.1007\/S00253-021-11684-2\/FIGURES\/3","journal-title":"Appl Microbiol Biotechnol"},{"key":"12270_CR29","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1111\/J.1472-765X.2007.02275.X","volume":"46","author":"T Prado","year":"2008","unstructured":"Prado T, Pereira WC, Silva DM, Seki LM, Carvalho APDA, Asensi MD (2008) Detection of extended-spectrum \u03b2-lactamase-producing Klebsiella pneumoniae in effluents and sludge of a hospital sewage treatment plant. Lett Appl Microbiol 46:136\u2013141. https:\/\/doi.org\/10.1111\/J.1472-765X.2007.02275.X","journal-title":"Lett Appl Microbiol"},{"key":"12270_CR30","doi-asserted-by":"publisher","unstructured":"Ramirez MS, Traglia GM, Lin DL, Tran T, Tolmasky ME (2014) Plasmid-mediated antibiotic resistance and virulence in gram-negatives: the Klebsiella pneumoniae\u00a0paradigm. Microbiology Spectrum 2(5):1\u201315. https:\/\/doi.org\/10.1128\/microbiolspec.PLAS-0016-2013","DOI":"10.1128\/microbiolspec.PLAS-0016-2013"},{"key":"12270_CR31","doi-asserted-by":"publisher","first-page":"113831","DOI":"10.1016\/J.IJHEH.2021.113831","volume":"237","author":"D Rolbiecki","year":"2021","unstructured":"Rolbiecki D, Harnisz M, Korzeniewska E, Buta M, Hubeny J, Zieli\u0144ski W (2021) Detection of carbapenemase-producing, hypervirulent Klebsiella spp. in wastewater and their potential transmission to river water and WWTP employees. Int J Hyg Environ Health 237:113831. https:\/\/doi.org\/10.1016\/J.IJHEH.2021.113831","journal-title":"Int J Hyg Environ Health"},{"key":"12270_CR32","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1016\/J.CMI.2017.11.001","volume":"24","author":"JWA Rossen","year":"2018","unstructured":"Rossen JWA, Friedrich AW, Moran-Gilad J (2018) Practical issues in implementing whole-genome-sequencing in routine diagnostic microbiology. Clin Microbiol Infect 24:355\u2013360. https:\/\/doi.org\/10.1016\/J.CMI.2017.11.001","journal-title":"Clin Microbiol Infect"},{"key":"12270_CR33","doi-asserted-by":"publisher","unstructured":"Sakkas H, Bozidis P, Ilia A, Mpekoulis G, Papadopoulou C (2019) Antimicrobial resistance in bacterial pathogens and detection of carbapenemases in Klebsiella pneumoniae isolates from hospital wastewater. Antibiotics 8, Page 85 8:85. https:\/\/doi.org\/10.3390\/ANTIBIOTICS8030085","DOI":"10.3390\/ANTIBIOTICS8030085"},{"key":"12270_CR34","doi-asserted-by":"publisher","unstructured":"Salim A, Babu P, Mohan K, Moorthy M, Raj D, Kallampillil Thirumeni S, Suresh S, Madhavan A, Nair BG, Chattopadhyay S, Pal S (2019) Draft genome sequence of an\u00a0Escherichia coli sequence type 155 strain isolated from sewage in Kerala, India. Microbiology Resource Announcements 8(27):e01707-18. https:\/\/doi.org\/10.1128\/MRA.01707-18","DOI":"10.1128\/MRA.01707-18"},{"key":"12270_CR35","doi-asserted-by":"publisher","first-page":"100314","DOI":"10.1016\/J.RINENG.2021.100314","volume":"13","author":"A Salim","year":"2022","unstructured":"Salim A, Sindhu Shetty K, Febin H, Sameed N, Pal S, Nair BG, Madhavan A (2022) Lytics broadcasting system: a novel approach to disseminate bacteriophages for disinfection and biogenic hydrogen sulphide removal tested in synthetic sewage. Results Eng 13:100314. https:\/\/doi.org\/10.1016\/J.RINENG.2021.100314","journal-title":"Results Eng"},{"key":"12270_CR36","doi-asserted-by":"publisher","first-page":"10115","DOI":"10.1007\/S00253-016-7881-1\/FIGURES\/4","volume":"100","author":"K Stojowska-Sw\u0119drzy\u0144ska","year":"2016","unstructured":"Stojowska-Sw\u0119drzy\u0144ska K, Krawczyk B (2016) A new assay for the simultaneous identification and differentiation of Klebsiella oxytoca strains. Appl Microbiol Biotechnol 100:10115\u201310123. https:\/\/doi.org\/10.1007\/S00253-016-7881-1\/FIGURES\/4","journal-title":"Appl Microbiol Biotechnol"},{"key":"12270_CR37","doi-asserted-by":"publisher","unstructured":"Subhash S, Babu P, Vijayakumar A, Suresh RA, Madhavan A, Nair BG, Pal S (2022) Aspergillus niger culture filtrate (ACF) mediated biocontrol of enteric pathogens in wastewater. Water 14, Page 119 14:119. https:\/\/doi.org\/10.3390\/W14010119","DOI":"10.3390\/W14010119"},{"key":"12270_CR38","doi-asserted-by":"publisher","first-page":"1134","DOI":"10.1007\/s12257-011-0513-9","volume":"16","author":"M Valan Arasu","year":"2011","unstructured":"Valan Arasu M, Kumar V, Ashok S, Song H, Rathnasingh C, Jong Lee H, Seung D, Park S (2011) Isolation and characterization of the new Klebsiella pneumoniae J2B strain showing improved growth characteristics with reduced lipopolysaccharide formation. Biotechnol Bioproc Eng 16:1134\u20131143. https:\/\/doi.org\/10.1007\/s12257-011-0513-9","journal-title":"Biotechnol Bioproc Eng"},{"key":"12270_CR39","doi-asserted-by":"publisher","first-page":"936","DOI":"10.1128\/JCM.20.5.936-941.1984","volume":"20","author":"E van Kregten","year":"1984","unstructured":"van Kregten E, Westerdaal NAC, Willers JMN (1984) New, simple medium for selective recovery of Klebsiella pneumoniae and Klebsiella oxytoca from human feces. J Clin Microbiol 20:936\u2013941. https:\/\/doi.org\/10.1128\/JCM.20.5.936-941.1984","journal-title":"J Clin Microbiol"},{"key":"12270_CR40","doi-asserted-by":"publisher","unstructured":"Varshney S, Sharma S, Gupta D (2022) Surveillance of bacterial load and multi-drug resistant bacteria on bedsheets in a primary health care unit. Int J Environ Health Res 32(9):2040\u20132051. https:\/\/doi.org\/10.1080\/09603123.2021.1935780","DOI":"10.1080\/09603123.2021.1935780"},{"key":"12270_CR41","doi-asserted-by":"publisher","unstructured":"Wyres KL, Lam MMC, Holt KE (2020) Population genomics of Klebsiella pneumoniae. Nat Rev Microbiol 18:6 18:344\u2013359. https:\/\/doi.org\/10.1038\/s41579-019-0315-1","DOI":"10.1038\/s41579-019-0315-1"},{"key":"12270_CR42","doi-asserted-by":"publisher","unstructured":"Zhou K, Lokate M, Deurenberg RH, Tepper M, Arends JP, Raangs EGC, Lo-Ten-Foe J, Grundmann H, Rossen JWA, Friedrich AW (2016) Use of whole-genome sequencing to trace, control and characterize the regional expansion of extended-spectrum \u03b2-lactamase producing ST15 Klebsiella pneumoniae. Sci Rep 6:1 6:1\u201310. https:\/\/doi.org\/10.1038\/srep20840","DOI":"10.1038\/srep20840"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-022-12270-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00253-022-12270-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-022-12270-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T02:05:33Z","timestamp":1670378733000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00253-022-12270-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,16]]},"references-count":42,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["12270"],"URL":"https:\/\/doi.org\/10.1007\/s00253-022-12270-w","relation":{},"ISSN":["0175-7598","1432-0614"],"issn-type":[{"value":"0175-7598","type":"print"},{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,16]]},"assertion":[{"value":"1 September 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 October 2022","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 October 2022","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 November 2022","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors have no commercial interests in any material discussed in this article.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This article does not contain any studies with human participants or animals performed by any authors.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}