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PCC 7120) were cultivated to produce cyanophycin, a nitrogen reserve compound, under nitrogen fixing conditions. In preliminary continuous experiments, <jats:italic>Nostoc<\/jats:italic> sp. was shown to be more efficient, accumulating a higher amount of cyanophycin and showing a greater capability to fix atmospheric nitrogen in the biomass (67 mg<jats:sub>N<\/jats:sub> d<jats:sup>\u22121<\/jats:sup> of fixed nitrogen per liter of culture). The operating conditions were then optimized to maximize the cyanophycin productivity: the effect of incident light intensity, residence time and nitrogen availability were investigated. Nitrogen availability and\/or pH played a major role with respect to biomass production, whereas phosphorus limitation was the main variable to maximize cyanophycin accumulation. In this way, it was possible to achieve a stable and continuous production of cyanophycin (CGP) under diazotrophic conditions, obtaining a maximum cyanophycin productivity of 15 mg<jats:sub>CGP<\/jats:sub> L<jats:sup>\u22121<\/jats:sup> d<jats:sup>\u22121<\/jats:sup>.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Key points<\/jats:title>\n                <jats:p>\u2022 <jats:italic>Diazotrophic cyanobacteria produce stable amount of cyanophycin in continuous PBR.<\/jats:italic><\/jats:p>\n                <jats:p>\u2022 <jats:italic>Nostoc sp. proved to be more efficient in producing cyanophycin than Anabaena sp.<\/jats:italic><\/jats:p>\n                <jats:p>\u2022 <jats:italic>P deprivation is the major variable to increase cyanophycin productivity in continuous.<\/jats:italic><\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s00253-022-12292-4","type":"journal-article","created":{"date-parts":[[2022,11,26]],"date-time":"2022-11-26T22:05:11Z","timestamp":1669500311000},"page":"97-110","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Fixing N2 into cyanophycin: continuous cultivation of Nostoc sp. 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