{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:16:28Z","timestamp":1792782988607,"version":"4.5.0"},"reference-count":59,"publisher":"Springer Science and Business Media LLC","issue":"7-8","license":[{"start":{"date-parts":[[2023,2,23]],"date-time":"2023-02-23T00:00:00Z","timestamp":1677110400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2023,2,23]],"date-time":"2023-02-23T00:00:00Z","timestamp":1677110400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32072892"],"award-info":[{"award-number":["32072892"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010038","name":"Earmarked Fund for China Agriculture Research System","doi-asserted-by":"publisher","award":["CARS-40"],"award-info":[{"award-number":["CARS-40"]}],"id":[{"id":"10.13039\/501100010038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010246","name":"Postdoctoral Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["1501075C"],"award-info":[{"award-number":["1501075C"]}],"id":[{"id":"10.13039\/501100010246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013088","name":"Qinglan Project of Jiangsu Province of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013088","id-type":"DOI","asserted-by":"publisher"}]},{"name":"High-end talent support program of Yangzhou University","id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"published-print":{"date-parts":[[2023,4]]},"DOI":"10.1007\/s00253-023-12433-3","type":"journal-article","created":{"date-parts":[[2023,2,23]],"date-time":"2023-02-23T04:03:56Z","timestamp":1677125036000},"page":"2437-2450","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Monoclonal antibody targeting a novel linear epitope on nucleoprotein confers pan-reactivity to influenza A virus"],"prefix":"10.1007","volume":"107","author":[{"given":"Min","family":"Gu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Jiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suhan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanchen","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhichuang","family":"Ge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kairui","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongchang","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenming","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pinghu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoquan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunlin","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9924-6646","authenticated-orcid":false,"given":"Xiufan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,2,23]]},"reference":[{"issue":"3","key":"12433_CR1","doi-asserted-by":"publisher","first-page":"a038737","DOI":"10.1101\/cshperspect.a038737","volume":"11","author":"TK Anderson","year":"2021","unstructured":"Anderson TK, Chang J, Arendsee ZW, Venkatesh D, Souza CK, Kimble JB, Lewis NS, Davis CT, Vincent AL (2021) Swine influenza A viruses and the tangled relationship with humans. Cold Spring Harb Perspect Med 11(3):a038737","journal-title":"Cold Spring Harb Perspect Med"},{"issue":"6","key":"12433_CR2","doi-asserted-by":"publisher","first-page":"156","DOI":"10.3390\/microorganisms7060156","volume":"7","author":"KJC Antigua","year":"2019","unstructured":"Antigua KJC, Choi WS, Baek YH, Song MS (2019) The emergence and decennary distribution of clade 2.3.4.4 HPAI H5Nx. Microorganisms 7(6):156","journal-title":"Microorganisms"},{"issue":"21","key":"12433_CR3","doi-asserted-by":"publisher","first-page":"11953","DOI":"10.3390\/ijms222111953","volume":"22","author":"SMB Asdaq","year":"2021","unstructured":"Asdaq SMB, Rabbani SI, Alkahtani M, Aldohyan MM, Alabdulsalam AM, Alshammari MS, Alajlan SA, Binrokan A, Mohzari Y, Alrashed A, Alshammari MK, Imran M, Nayeem N (2021) A patent review on the therapeutic application of monoclonal antibodies in COVID-19. Int J Mol Sci 22(21):11953","journal-title":"Int J Mol Sci"},{"issue":"6","key":"12433_CR4","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1089\/mab.2013.0053","volume":"32","author":"S Bhat","year":"2013","unstructured":"Bhat S, Bhatia S, Sood R, Bhatnagar H, Pateriya A, Venkatesh G (2013) Production and characterization of monoclonal antibodies against nucleoprotein of avian influenza virus. Monoclon Antib Immunodiagn Immunother 32(6):413\u2013418","journal-title":"Monoclon Antib Immunodiagn Immunother"},{"issue":"8","key":"12433_CR5","doi-asserted-by":"publisher","first-page":"1333","DOI":"10.1128\/CVI.00339-13","volume":"20","author":"R Bodewes","year":"2013","unstructured":"Bodewes R, Geelhoed-Mieras MM, Wrammert J, Ahmed R, Wilson PC, Fouchier RA, Osterhaus AD, Rimmelzwaan GF (2013) In vitro assessment of the immunological significance of a human monoclonal antibody directed to the influenza a virus nucleoprotein. Clin Vaccine Immunol 20(8):1333\u20131337","journal-title":"Clin Vaccine Immunol"},{"issue":"5","key":"12433_CR6","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1016\/j.coph.2012.08.001","volume":"12","author":"NA Buss","year":"2012","unstructured":"Buss NA, Henderson SJ, McFarlane M, Shenton JM, de Haan L (2012) Monoclonal antibody therapeutics: history and future. Curr Opin Pharmacol 12(5):615\u2013622","journal-title":"Curr Opin Pharmacol"},{"issue":"6","key":"12433_CR7","doi-asserted-by":"publisher","first-page":"a038588","DOI":"10.1101\/cshperspect.a038588","volume":"10","author":"S Carnaccini","year":"2020","unstructured":"Carnaccini S, Perez DR (2020) H9 influenza viruses: an emerging challenge. Cold Spring Harb Perspect Med 10(6):a038588","journal-title":"Cold Spring Harb Perspect Med"},{"issue":"6","key":"12433_CR8","doi-asserted-by":"publisher","first-page":"1188","DOI":"10.1101\/gr.849004","volume":"14","author":"GE Crooks","year":"2004","unstructured":"Crooks GE, Hon G, Chandonia JM, Brenner SE (2004) WebLogo: a sequence logo generator. Genome Res 14(6):1188\u20131190","journal-title":"Genome Res"},{"key":"12433_CR9","doi-asserted-by":"publisher","first-page":"1816","DOI":"10.3389\/fmicb.2019.01816","volume":"10","author":"L Cui","year":"2019","unstructured":"Cui L, Zheng W, Li M, Bai X, Yang W, Li J, Fan W, Gao GF, Sun L, Liu W (2019) Phosphorylation status of tyrosine 78 residue regulates the nuclear export and ubiquitination of influenza A virus nucleoprotein. Front Microbiol 10:1816","journal-title":"Front Microbiol"},{"issue":"1","key":"12433_CR10","doi-asserted-by":"publisher","first-page":"143","DOI":"10.3201\/eid2601.191144","volume":"26","author":"YM Deng","year":"2018","unstructured":"Deng YM, Wong FYK, Spirason N, Kaye M, Beazley R, Grau MLL, Shan S, Stevens V, Subbarao K, Sullivan S, Barr IG (2018) Dhanasekaran V (2020) Locally acquired human infection with swine-origin influenza A(H3N2) variant virus, Australia. Emerg Infect Dis 26(1):143\u2013147","journal-title":"Emerg Infect Dis"},{"issue":"Pt 5","key":"12433_CR11","doi-asserted-by":"publisher","first-page":"1162","DOI":"10.1099\/vir.0.029777-0","volume":"92","author":"JD Doucet","year":"2011","unstructured":"Doucet JD, Forget MA, Grange C, Rouxel RN, Arbour N, von Messling V, Lapointe R (2011) Endogenously expressed matrix protein M1 and nucleoprotein of influenza A are efficiently presented by class I and class II major histocompatibility complexes. J Gen Virol 92(Pt 5):1162\u20131171","journal-title":"J Gen Virol"},{"issue":"1","key":"12433_CR12","doi-asserted-by":"publisher","first-page":"9","DOI":"10.4161\/19420862.2015.989042","volume":"7","author":"DM Ecker","year":"2015","unstructured":"Ecker DM, Jones SD, Levine HL (2015) The therapeutic monoclonal antibody market. MAbs 7(1):9\u201314","journal-title":"MAbs"},{"issue":"9","key":"12433_CR13","doi-asserted-by":"publisher","first-page":"2104","DOI":"10.1099\/jgv.0.000518","volume":"97","author":"Y Fujimoto","year":"2016","unstructured":"Fujimoto Y, Tomioka Y, Takakuwa H, Uechi GI, Yabuta T, Ozaki K, Suyama H, Yamamoto S, Morimatsu M, Mai LQ, Yamashiro T, Ito T, Otsuki K, Ono E (2016) Cross-protective potential of anti-nucleoprotein human monoclonal antibodies against lethal influenza A virus infection. J Gen Virol 97(9):2104\u20132116","journal-title":"J Gen Virol"},{"issue":"4","key":"12433_CR14","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1080\/15321819.2018.1515775","volume":"39","author":"Y Gao","year":"2018","unstructured":"Gao Y, Huang X, Zhu Y, Lv Z (2018) A brief review of monoclonal antibody technology and its representative applications in immunoassays. J Immunoassay Immunochem 39(4):351\u2013364","journal-title":"J Immunoassay Immunochem"},{"issue":"2","key":"12433_CR15","doi-asserted-by":"publisher","first-page":"730","DOI":"10.1111\/tbed.13736","volume":"68","author":"Z Ge","year":"2021","unstructured":"Ge Z, Gu M, Cai T, Liu K, Gao R, Liu D, Sun W, Li X, Shi L, Liu J, Wang X, Hu J, Liu X, Hu S, Chen S, Peng D, Jiao X, Liu X (2021) Phylogenetic tracing and biological characterization of a novel clade 2.3.2.1 reassortant of H5N6 subtype avian influenza virus in China. Transbound Emerg Dis 68(2):730\u2013741","journal-title":"Transbound Emerg Dis"},{"key":"12433_CR16","doi-asserted-by":"publisher","first-page":"105205","DOI":"10.1016\/j.meegid.2022.105205","volume":"98","author":"Z Ge","year":"2022","unstructured":"Ge Z, Xu L, Hu X, Zhu S, Zhao Y, Li Y, Liu K, Gao R, Wang X, Hu J, Liu X, Hu S, Peng D, Gu M, Liu X (2022) Phylogenetic and phenotypic characterization of two novel clade 2.3.2.1 H5N2 subtype avian influenza viruses from chickens in China. Infect Genet Evol 98:105205","journal-title":"Infect Genet Evol"},{"issue":"1","key":"12433_CR17","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1186\/s13567-017-0453-2","volume":"48","author":"M Gu","year":"2017","unstructured":"Gu M, Xu L, Wang X, Liu X (2017) Current situation of H9N2 subtype avian influenza in China. Vet Res 48(1):49","journal-title":"Vet Res"},{"issue":"6","key":"12433_CR18","doi-asserted-by":"publisher","first-page":"995","DOI":"10.1002\/jmv.23812","volume":"86","author":"X Gui","year":"2014","unstructured":"Gui X, Ge P, Wang X, Yang K, Yu H, Zhao Q, Chen Y, Xia N (2014) Identification of a highly conserved and surface exposed B-cell epitope on the nucleoprotein of influenza A virus. J Med Virol 86(6):995\u20131002","journal-title":"J Med Virol"},{"issue":"6","key":"12433_CR19","doi-asserted-by":"publisher","first-page":"1172","DOI":"10.3390\/v14061172","volume":"14","author":"X Huang","year":"2022","unstructured":"Huang X, Huang J, Yin G, Cai Y, Chen M, Hu J, Feng X (2022) Identification of NP protein-specific B-cell epitopes for H9N2 subtype of avian influenza virus. Viruses 14(6):1172","journal-title":"Viruses"},{"issue":"9","key":"12433_CR20","doi-asserted-by":"publisher","first-page":"809","DOI":"10.1016\/j.tim.2018.05.013","volume":"26","author":"EC Hutchinson","year":"2018","unstructured":"Hutchinson EC (2018) Influenza Virus. Trends Microbiol 26(9):809\u2013810","journal-title":"Trends Microbiol"},{"key":"12433_CR21","doi-asserted-by":"crossref","unstructured":"Jespersen MC, Peters B, Nielsen M, Marcatili P (2017) BepiPred-2.0: improving sequence-based B-cell epitope prediction using conformational epitopes. Nucleic Acids Res 45(W1):W24-W29","DOI":"10.1093\/nar\/gkx346"},{"issue":"1","key":"12433_CR22","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1111\/irv.12412","volume":"11","author":"U Joseph","year":"2017","unstructured":"Joseph U, Su YC, Vijaykrishna D, Smith GJ (2017) The ecology and adaptive evolution of influenza A interspecies transmission. Influenza Other Respir Viruses 11(1):74\u201384","journal-title":"Influenza Other Respir Viruses"},{"issue":"6","key":"12433_CR23","doi-asserted-by":"publisher","first-page":"1547","DOI":"10.1093\/molbev\/msy096","volume":"35","author":"S Kumar","year":"2018","unstructured":"Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35(6):1547\u20131549","journal-title":"Mol Biol Evol"},{"issue":"S1","key":"12433_CR24","doi-asserted-by":"publisher","first-page":"269","DOI":"10.4142\/jvs.2017.18.S1.269","volume":"18","author":"DH Lee","year":"2017","unstructured":"Lee DH, Bertran K, Kwon JH, Swayne DE (2017) Evolution, global spread, and pathogenicity of highly pathogenic avian influenza H5Nx clade 2.3.4.4. J Vet Sci 18(S1):269\u2013280","journal-title":"J Vet Sci"},{"issue":"2","key":"12433_CR25","doi-asserted-by":"publisher","first-page":"a038679","DOI":"10.1101\/cshperspect.a038679","volume":"11","author":"DH Lee","year":"2021","unstructured":"Lee DH, Criado MF, Swayne DE (2021) Pathobiological origins and evolutionary history of highly pathogenic avian influenza viruses. Cold Spring Harb Perspect Med 11(2):a038679","journal-title":"Cold Spring Harb Perspect Med"},{"issue":"5","key":"12433_CR26","doi-asserted-by":"publisher","first-page":"2668","DOI":"10.3390\/v7052668","volume":"7","author":"J Li","year":"2015","unstructured":"Li J, Yu M, Zheng W, Liu W (2015) Nucleocytoplasmic shuttling of influenza A virus proteins. Viruses 7(5):2668\u20132682","journal-title":"Viruses"},{"key":"12433_CR27","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.virol.2018.05.002","volume":"520","author":"Y Li","year":"2018","unstructured":"Li Y, Sun L, Zheng W, Madina M, Li J, Bi Y, Wang H, Liu W, Luo TR (2018) Phosphorylation and dephosphorylation of threonine 188 in nucleoprotein is crucial for the replication of influenza A virus. Virology 520:30\u201338","journal-title":"Virology"},{"issue":"1","key":"12433_CR28","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1093\/bmb\/ldz036","volume":"132","author":"YT Li","year":"2019","unstructured":"Li YT, Linster M, Mendenhall IH, Su YCF, Smith GJD (2019) Avian influenza viruses in humans: lessons from past outbreaks. Br Med Bull 132(1):81\u201395","journal-title":"Br Med Bull"},{"issue":"1","key":"12433_CR29","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1111\/tbed.13347","volume":"67","author":"J Li","year":"2020","unstructured":"Li J, Gu M, Liu K, Gao R, Sun W, Liu D, Jiang K, Zhong L, Wang X, Hu J, Hu S, Liu X, Shi W, Ren H, Peng D, Jiao X, Liu X (2020) Amino acid substitutions in antigenic region B of hemagglutinin play a critical role in the antigenic drift of subclade 2.3.4.4 highly pathogenic H5NX influenza viruses. Transbound Emerg Dis 67(1):263\u2013275","journal-title":"Transbound Emerg Dis"},{"issue":"5","key":"12433_CR30","first-page":"648","volume":"22","author":"F Liu","year":"2006","unstructured":"Liu F, Jin M, Zhang A, Dan B, Chen H (2006) Preparation and partial charaterization of monoclonal antibodies specific for nuclear protein of avian influenza virus. Chin J Cell Mol Immunol 22(5):648\u2013649 (in Chinese with English abstract)","journal-title":"Chin J Cell Mol Immunol"},{"issue":"4","key":"12433_CR31","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1007\/s11684-020-0814-5","volume":"15","author":"WJ Liu","year":"2021","unstructured":"Liu WJ, Xiao H, Dai L, Liu D, Chen J, Qi X, Bi Y, Shi Y, Gao GF, Liu Y (2021) Avian influenza A (H7N9) virus: from low pathogenic to highly pathogenic. Front Med 15(4):507\u2013527","journal-title":"Front Med"},{"key":"12433_CR32","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1007\/978-981-10-8456-0_5","volume":"88","author":"CY Lo","year":"2018","unstructured":"Lo CY, Tang YS, Shaw PC (2018) Structure and function of influenza virus ribonucleoprotein. Subcell Biochem 88:95\u2013128","journal-title":"Subcell Biochem"},{"issue":"2","key":"12433_CR33","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1038\/s41579-018-0115-z","volume":"17","author":"JS Long","year":"2019","unstructured":"Long JS, Mistry B, Haslam SM, Barclay WS (2019) Host and viral determinants of influenza A virus species specificity. Nat Rev Microbiol 17(2):67\u201381","journal-title":"Nat Rev Microbiol"},{"issue":"1","key":"12433_CR34","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1186\/s13568-020-00988-7","volume":"10","author":"S Luo","year":"2020","unstructured":"Luo S, Deng X, Xie Z, Huang J, Zhang M, Li M, Xie L, Li D, Fan Q, Wang S, Zeng T, Zhang Y, Xie Z (2020) Production and identification of monoclonal antibodies and development of a sandwich ELISA for detection of the H3-subtype avian influenza virus antigen. AMB Express 10(1):49","journal-title":"AMB Express"},{"key":"12433_CR35","doi-asserted-by":"publisher","first-page":"1695","DOI":"10.3389\/fimmu.2018.01695","volume":"9","author":"B Manavalan","year":"2018","unstructured":"Manavalan B, Govindaraj RG, Shin TH, Kim MO, Lee G (2018) iBCE-EL: a new ensemble learning framework for improved linear B-Cell epitope prediction. Front Immunol 9:1695","journal-title":"Front Immunol"},{"key":"12433_CR36","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1146\/annurev-med-120617-041310","volume":"71","author":"R Nachbagauer","year":"2020","unstructured":"Nachbagauer R, Palese P (2020) Is a universal influenza virus vaccine possible? Annu Rev Med 71:315\u2013327","journal-title":"Annu Rev Med"},{"issue":"10","key":"12433_CR37","doi-asserted-by":"publisher","first-page":"3638","DOI":"10.1096\/fj.08-112110","volume":"22","author":"AK Ng","year":"2008","unstructured":"Ng AK, Zhang H, Tan K, Li Z, Liu JH, Chan PK, Li SM, Chan WY, Au SW, Joachimiak A, Walz T, Wang JH, Shaw PC (2008) Structure of the influenza virus A H5N1 nucleoprotein: implications for RNA binding, oligomerization, and vaccine design. FASEB J 22(10):3638\u20133647","journal-title":"FASEB J"},{"issue":"5","key":"12433_CR38","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1007\/s11427-009-0064-x","volume":"52","author":"AK Ng","year":"2009","unstructured":"Ng AK, Wang JH, Shaw PC (2009) Structure and sequence analysis of influenza A virus nucleoprotein. Sci China C Life Sci 52(5):439\u2013449","journal-title":"Sci China C Life Sci"},{"issue":"3","key":"12433_CR39","doi-asserted-by":"publisher","first-page":"e33097","DOI":"10.1371\/journal.pone.0033097","volume":"7","author":"S Nutter","year":"2012","unstructured":"Nutter S, Cheung M, Adler-Shohet FC, Krusel K, Vogel K, Meyers H (2012) Evaluation of indirect fluorescent antibody assays compared to rapid influenza diagnostic tests for the detection of pandemic influenza A (H1N1) pdm09. PLoS One 7(3):e33097","journal-title":"PLoS One"},{"issue":"3","key":"12433_CR40","doi-asserted-by":"publisher","first-page":"939","DOI":"10.3201\/eid2703.201863","volume":"27","author":"A Parys","year":"2019","unstructured":"Parys A, Vandoorn E, King J, Graaf A, Pohlmann A, Beer M, Harder T (2019) Van Reeth K (2021) Human infection with Eurasian avian-like swine influenza A(H1N1) virus, the Netherlands. Emerg Infect Dis 27(3):939\u2013943","journal-title":"Emerg Infect Dis"},{"issue":"1","key":"12433_CR41","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1038\/nrmicro.2017.146","volume":"16","author":"VN Petrova","year":"2018","unstructured":"Petrova VN, Russell CA (2018) The evolution of seasonal influenza viruses. Nat Rev Microbiol 16(1):60","journal-title":"Nat Rev Microbiol"},{"issue":"5","key":"12433_CR42","doi-asserted-by":"publisher","first-page":"809","DOI":"10.4014\/jmb.1801.01002","volume":"28","author":"NH Phuong","year":"2018","unstructured":"Phuong NH, Kwak C, Heo CK, Cho EW, Yang J, Poo H (2018) Development and characterization of monoclonal antibodies against nucleoprotein for diagnosis of influenza A virus. J Microbiol Biotechnol 28(5):809\u2013815","journal-title":"J Microbiol Biotechnol"},{"issue":"2","key":"12433_CR43","doi-asserted-by":"publisher","first-page":"548","DOI":"10.1073\/pnas.1422456112","volume":"112","author":"J Pu","year":"2015","unstructured":"Pu J, Wang S, Yin Y, Zhang G, Carter RA, Wang J, Xu G, Sun H, Wang M, Wen C, Wei Y, Wang D, Zhu B, Lemmon G, Jiao Y, Duan S, Wang Q, Du Q, Sun M, Bao J, Sun Y, Zhao J, Zhang H, Wu G, Liu J, Webster RG (2015) Evolution of the H9N2 influenza genotype that facilitated the genesis of the novel H7N9 virus. Proc Natl Acad Sci U S A 112(2):548\u2013553","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"11","key":"12433_CR44","doi-asserted-by":"publisher","first-page":"1087","DOI":"10.1056\/NEJMc2112416","volume":"386","author":"X Qi","year":"2022","unstructured":"Qi X, Qiu H, Hao S, Zhu F, Huang Y, Xu K, Yu H, Wang D, Zhou L, Dai Q, Zhou Y, Wang S, Huang H, Yu S, Huo X, Chen K, Liu J, Hu J, Wu M, Bao C (2022) Human infection with an avian-origin influenza A (H10N3) Virus. N Engl J Med 386(11):1087\u20131088","journal-title":"N Engl J Med"},{"key":"12433_CR45","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1007\/978-1-60327-118-9_29","volume":"409","author":"S Saha","year":"2007","unstructured":"Saha S, Raghava GP (2007) Prediction methods for B-cell epitopes. Methods Mol Biol 409:387\u2013394","journal-title":"Methods Mol Biol"},{"issue":"2","key":"12433_CR46","doi-asserted-by":"publisher","first-page":"125","DOI":"10.3390\/vaccines9020125","volume":"9","author":"M Sangesland","year":"2021","unstructured":"Sangesland M, Lingwood D (2021) Antibody focusing to conserved sites of vulnerability: the immunological pathways for \u201cuniversal\u201d influenza vaccines. Vaccines (Basel) 9(2):125","journal-title":"Vaccines (Basel)"},{"issue":"3","key":"12433_CR47","doi-asserted-by":"publisher","first-page":"521","DOI":"10.3390\/v14030521","volume":"14","author":"M Santak","year":"2022","unstructured":"Santak M, Matic Z (2022) The role of nucleoprotein in immunity to human negative-stranded RNA viruses-not just another brick in the viral nucleocapsid. Viruses 14(3):521","journal-title":"Viruses"},{"issue":"2","key":"12433_CR48","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1637\/8520-111708-Reg.1","volume":"53","author":"E Spackman","year":"2009","unstructured":"Spackman E, Pantin-Jackwood MJ, Swayne DE, Suarez DL (2009) An evaluation of avian influenza diagnostic methods with domestic duck specimens. Avian Dis 53(2):276\u2013280","journal-title":"Avian Dis"},{"key":"12433_CR49","doi-asserted-by":"publisher","first-page":"2069","DOI":"10.3389\/fmicb.2018.02069","volume":"9","author":"Z Sun","year":"2018","unstructured":"Sun Z, Shi B, Meng F, Ma R, Hu Q, Qin T, Chen S, Peng D, Liu X (2018) Development of a colloidal gold-based immunochromatographic strip for rapid detection of H7N9 influenza viruses. Front Microbiol 9:2069","journal-title":"Front Microbiol"},{"issue":"29","key":"12433_CR50","doi-asserted-by":"publisher","first-page":"17204","DOI":"10.1073\/pnas.1921186117","volume":"117","author":"H Sun","year":"2020","unstructured":"Sun H, Xiao Y, Liu J, Wang D, Li F, Wang C, Li C, Zhu J, Song J, Sun H, Jiang Z, Liu L, Zhang X, Wei K, Hou D, Pu J, Sun Y, Tong Q, Bi Y, Chang KC, Liu S, Gao GF, Liu J (2020) Prevalent Eurasian avian-like H1N1 swine influenza virus with 2009 pandemic viral genes facilitating human infection. Proc Natl Acad Sci U S A 117(29):17204\u201317210","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"7","key":"12433_CR51","doi-asserted-by":"publisher","first-page":"739","DOI":"10.3390\/vaccines9070739","volume":"9","author":"MP Tan","year":"2021","unstructured":"Tan MP, Tan WS, Mohamed Alitheen NB, Yap WB (2021) M2e-based influenza vaccines with nucleoprotein: a review. Vaccines (Basel) 9(7):739","journal-title":"Vaccines (Basel)"},{"issue":"2","key":"12433_CR52","doi-asserted-by":"publisher","first-page":"1452","DOI":"10.1128\/JVI.02332-14","volume":"89","author":"L Turrell","year":"2015","unstructured":"Turrell L, Hutchinson EC, Vreede FT, Fodor E (2015) Regulation of influenza A virus nucleoprotein oligomerization by phosphorylation. J Virol 89(2):1452\u20131455","journal-title":"J Virol"},{"issue":"1","key":"12433_CR53","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1111\/imcb.12508","volume":"100","author":"HA Vanderven","year":"2022","unstructured":"Vanderven HA, Esterbauer R, Jegaskanda S, Tan HX, Wheatley AK, Kent SJ (2022) Poor protective potential of influenza nucleoprotein antibodies despite wide prevalence. Immunol Cell Biol 100(1):49\u201360","journal-title":"Immunol Cell Biol"},{"issue":"5","key":"12433_CR54","doi-asserted-by":"publisher","first-page":"1124","DOI":"10.1007\/s12250-021-00383-x","volume":"36","author":"Y Wu","year":"2021","unstructured":"Wu Y, Hu J, Jin X, Li X, Wang J, Zhang M, Chen J, Xie S, Qi W, Liao M, Jia W (2021) Accelerated evolution of H7N9 subtype influenza virus under vaccination pressure. Virol Sin 36(5):1124\u20131132","journal-title":"Virol Sin"},{"issue":"12","key":"12433_CR55","first-page":"53","volume":"50","author":"Q Xiao","year":"2018","unstructured":"Xiao Q, Yao L, Bi Z, Lei J, Yan Y, Yan L (2018) Preparation and identification of monoclonal antibodies against NP protein of avian influenza virus. Animal Husbandry & Veterinary Medicine 50(12):53\u201358 (in Chinese with English abstract)","journal-title":"Animal Husbandry & Veterinary Medicine"},{"issue":"2","key":"12433_CR56","doi-asserted-by":"publisher","first-page":"392","DOI":"10.1073\/pnas.1522643113","volume":"113","author":"H Yang","year":"2016","unstructured":"Yang H, Chen Y, Qiao C, He X, Zhou H, Sun Y, Yin H, Meng S, Liu L, Zhang Q, Kong H, Gu C, Li C, Bu Z, Kawaoka Y, Chen H (2016) Prevalence, genetics, and transmissibility in ferrets of Eurasian avian-like H1N1 swine influenza viruses. Proc Natl Acad Sci U S A 113(2):392\u2013397","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"4","key":"12433_CR57","doi-asserted-by":"publisher","first-page":"e1009561","DOI":"10.1371\/journal.ppat.1009561","volume":"17","author":"X Yin","year":"2021","unstructured":"Yin X, Deng G, Zeng X, Cui P, Hou Y, Liu Y, Fang J, Pan S, Wang D, Chen X, Zhang Y, Wang X, Tian G, Li Y, Chen Y, Liu L, Suzuki Y, Guan Y, Li C, Shi J, Chen H (2021) Genetic and biological properties of H7N9 avian influenza viruses detected after application of the H7N9 poultry vaccine in China. PLoS Pathog 17(4):e1009561","journal-title":"PLoS Pathog"},{"issue":"1","key":"12433_CR58","doi-asserted-by":"publisher","first-page":"13318","DOI":"10.1038\/s41598-020-70135-6","volume":"10","author":"CM Yu","year":"2020","unstructured":"Yu CM, Chen IC, Tung CP, Peng HP, Jian JW, Chiu YK, Tsou YL, Chen HS, Huang YJ, Hsiao WW, Wang YA, Yang AS (2020) A panel of anti-influenza virus nucleoprotein antibodies selected from phage-displayed synthetic antibody libraries with rapid diagnostic capability to distinguish diverse influenza virus subtypes. Sci Rep 10(1):13318","journal-title":"Sci Rep"},{"issue":"6","key":"12433_CR59","doi-asserted-by":"publisher","first-page":"e0178634","DOI":"10.1371\/journal.pone.0178634","volume":"12","author":"Z Zhang","year":"2017","unstructured":"Zhang Z, Liu D, Sun W, Liu J, He L, Hu J, Gu M, Wang X, Liu X, Hu S, Chen S, Peng D, Liu X (2017) Multiplex one-step Real-time PCR by Taqman-MGB method for rapid detection of pan and H5 subtype avian influenza viruses. PLoS One 12(6):e0178634","journal-title":"PLoS One"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-023-12433-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00253-023-12433-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-023-12433-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T20:38:36Z","timestamp":1744144716000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00253-023-12433-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,23]]},"references-count":59,"journal-issue":{"issue":"7-8","published-print":{"date-parts":[[2023,4]]}},"alternative-id":["12433"],"URL":"https:\/\/doi.org\/10.1007\/s00253-023-12433-3","relation":{},"ISSN":["0175-7598","1432-0614"],"issn-type":[{"value":"0175-7598","type":"print"},{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,23]]},"assertion":[{"value":"16 November 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 January 2023","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 February 2023","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 February 2023","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The animal experiment was approved by the Institutional Animal Ethics Committee of Yangzhou University, China (permission number: SYXK (SU) 2017-0044), and complied with the animal care and ethics guidelines.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"The authors declare no competing interests","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}