{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T19:34:37Z","timestamp":1794080077701,"version":"4.8.0"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"24","license":[{"start":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T00:00:00Z","timestamp":1698278400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T00:00:00Z","timestamp":1698278400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"published-print":{"date-parts":[[2023,12]]},"DOI":"10.1007\/s00253-023-12808-6","type":"journal-article","created":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T09:02:12Z","timestamp":1698310932000},"page":"7463-7473","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Reconstitution of (1\u21923)-\u03b2-D-glucans measurement system using recombinant Limulus polyphemus Factor G"],"prefix":"10.1007","volume":"107","author":[{"given":"Yotaro","family":"Yamamoto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Kajiura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Fukuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Nishibu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhito","family":"Fujiyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9157-148X","authenticated-orcid":false,"given":"Takeshi","family":"Kitagawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,10,26]]},"reference":[{"key":"12808_CR1","doi-asserted-by":"publisher","first-page":"e958","DOI":"10.3791\/958","volume":"20","author":"P Armstrong","year":"2008","unstructured":"Armstrong P, Conrad M (2008) Blood collection from the American horseshoe crab, Limulus polyphemus. J Vis Exp 20:e958. https:\/\/doi.org\/10.3791\/958","journal-title":"J Vis Exp"},{"key":"12808_CR2","doi-asserted-by":"publisher","first-page":"1299","DOI":"10.1111\/myc.13170","volume":"63","author":"V Cento","year":"2020","unstructured":"Cento V, Alteri C, Mancini V, Gatti M, Lepera V, Mazza E, Moioli MC, Merli M, Colombo J, Orcese CA, Bielli A, Torri S, Gasparini LE, Vismara C, De Gasperi A, Brioschi P, Puoti M, Cairoli R, Lombardi G, Perno CF (2020) Quantification of 1,3-\u03b2-d-glucan by Wako \u03b2-glucan assay for rapid exclusion of invasive fungal infections in critical patients: a diagnostic test accuracy study. Mycoses 63:1299\u20131310. https:\/\/doi.org\/10.1111\/myc.13170","journal-title":"Mycoses"},{"key":"12808_CR3","doi-asserted-by":"publisher","first-page":"658144","DOI":"10.3389\/fmicb.2021.658144","volume":"12","author":"F Cui","year":"2021","unstructured":"Cui F, Luo P, Bai Y, Meng J (2021) A novel diagnostic method for invasive fungal disease using the Factor G alpha subunit from limulus polyphemus. Front Microbiol 12:658144. https:\/\/doi.org\/10.3389\/fmicb.2021.658144","journal-title":"Front Microbiol"},{"key":"12808_CR4","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1007\/978-90-481-9078-2_9","volume-title":"Endotoxins: structure, function and recognition","author":"JL Ding","year":"2010","unstructured":"Ding JL, Ho B (2010) Endotoxin detection \u2013 from limulus amebocyte lysate to recombinant Factor C. In: Wang X, Quinn PJ (eds) Endotoxins: structure, function and recognition. Springer, Netherlands, Dordrecht, pp 187\u2013208"},{"key":"12808_CR5","doi-asserted-by":"publisher","first-page":"105716","DOI":"10.1016\/j.ejps.2021.105716","volume":"159","author":"J Dubczak","year":"2021","unstructured":"Dubczak J, Reid N, Tsuchiya M (2021) Evaluation of limulus amebocyte lysate and recombinant endotoxin alternative assays for an assessment of endotoxin detection specificity. Eur J Pharm Sci 159:105716. https:\/\/doi.org\/10.1016\/j.ejps.2021.105716","journal-title":"Eur J Pharm Sci"},{"key":"12808_CR6","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1016\/j.pep.2014.01.009","volume":"95","author":"Y Fujii","year":"2014","unstructured":"Fujii Y, Kaneko M, Neyazaki M, Nogi T, Kato Y, Takagi J (2014) PA tag: a versatile protein tagging system using a super high affinity antibody against a dodecapeptide derived from human podoplanin. Protein Expr Purif 95:240\u2013247. https:\/\/doi.org\/10.1016\/j.pep.2014.01.009","journal-title":"Protein Expr Purif"},{"key":"12808_CR7","doi-asserted-by":"publisher","first-page":"1494","DOI":"10.1038\/nprot.2013.084","volume":"8","author":"BJ Haas","year":"2013","unstructured":"Haas BJ, Papanicolaou A, Yassour M, Grabherr M, Blood PD, Bowden J, Couger MB, Eccles D, Li B, Lieber M, MacManes MD, Ott M, Orvis J, Pochet N, Strozzi F, Weeks N, Westerman R, William T, Dewey CN et al (2013) De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis. Nat Protoc 8:1494\u20131512. https:\/\/doi.org\/10.1038\/nprot.2013.084","journal-title":"Nat Protoc"},{"key":"12808_CR8","doi-asserted-by":"publisher","first-page":"110","DOI":"10.2183\/pjab.83.110","volume":"83","author":"S Iwanaga","year":"2007","unstructured":"Iwanaga S (2007) Biochemical principle of Limulus test for detecting bacterial endotoxins. Proc Jpn. Acad Ser B Phys Biol Sci 83:110\u2013119. https:\/\/doi.org\/10.2183\/pjab.83.110","journal-title":"Acad Ser B Phys Biol Sci"},{"key":"12808_CR9","doi-asserted-by":"publisher","first-page":"3059","DOI":"10.1093\/nar\/gkf436","volume":"30","author":"K Katoh","year":"2002","unstructured":"Katoh K, Misawa K, Kuma K, Miyata T (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res 30:3059\u20133066. https:\/\/doi.org\/10.1093\/nar\/gkf436","journal-title":"Nucleic Acids Res"},{"key":"12808_CR10","first-page":"59","volume":"6","author":"S-i Kawabata","year":"2009","unstructured":"Kawabata S-i, Koshiba T, Shibata T (2009) The lipopolysaccharide-activated innate immune response network of the horseshoe crab. I.S.J. Invertebr Surviv J 6:59\u201377","journal-title":"Invertebr Surviv J"},{"key":"12808_CR11","doi-asserted-by":"publisher","first-page":"25987","DOI":"10.1074\/jbc.m114.586933","volume":"289","author":"Y Kobayashi","year":"2014","unstructured":"Kobayashi Y, Shiga T, Shibata T, Sako M, Maenaka K, Koshiba T, Mizumura H, Oda T, Kawabata S-I (2014) The N-terminal Arg residue is essential for autocatalytic activation of a lipopolysaccharide-responsive protease zymogen. J Biol Chem 289:25987\u201325995. https:\/\/doi.org\/10.1074\/jbc.m114.586933","journal-title":"J Biol Chem"},{"key":"12808_CR12","doi-asserted-by":"publisher","first-page":"19379","DOI":"10.1074\/jbc.m115.653196","volume":"290","author":"Y Kobayashi","year":"2015","unstructured":"Kobayashi Y, Takahashi T, Shibata T, Ikeda S, Koshiba T, Mizumura H, Oda T, Kawabata S-I (2015) Factor B is the second lipopolysaccharide-binding protease zymogen in the horseshoe crab coagulation cascade. J Biol Chem 290:19379\u201319386. https:\/\/doi.org\/10.1074\/jbc.m115.653196","journal-title":"J Biol Chem"},{"key":"12808_CR13","doi-asserted-by":"publisher","first-page":"1650","DOI":"10.1086\/647942","volume":"49","author":"S Koo","year":"2009","unstructured":"Koo S, Bryar JM, Page JH, Baden LR, Marty FM (2009) Diagnostic performance of the (1\u21923)-beta-D-glucan assay for invasive fungal disease. Clin Infect Dis 49:1650\u20131659","journal-title":"Clin Infect Dis"},{"key":"12808_CR14","doi-asserted-by":"publisher","first-page":"21393","DOI":"10.1038\/s41598-020-78425-9","volume":"10","author":"J Korn","year":"2020","unstructured":"Korn J, Sch\u00e4ckermann D, Kirmann T, Bertoglio F, Steinke S, Heisig J, Ruschig M, Rojas G, Langreder N, Wenzel EV, Roth KDR, Becker M, Meier D, van den Heuvel J, Hust M, D\u00fcbel S, Schubert M (2020) Baculovirus-free insect cell expression system for high yield antibody and antigen production. Sci Rep 10:21393. https:\/\/doi.org\/10.1038\/s41598-020-78425-9","journal-title":"Sci Rep"},{"key":"12808_CR15","doi-asserted-by":"publisher","unstructured":"Krisfalusi-Gannon J, Ali W, Dellinger K, Robertson L, Brady TE, Goddard MKM, Tinker-Kulberg R, Kepley CL, Dellinger AL (2018) The role of horseshoe crabs in the biomedical industry and recent trends impacting species sustainability. Front Mar Sci 5. https:\/\/doi.org\/10.3389\/fmars.2018.00185","DOI":"10.3389\/fmars.2018.00185"},{"key":"12808_CR16","doi-asserted-by":"publisher","first-page":"558","DOI":"10.1016\/0167-4838(83)90273-X","volume":"742","author":"R Lottenberg","year":"1983","unstructured":"Lottenberg R, Jackson CM (1983) Solution composition dependent variation in extinction coefficients for p-nitroaniline. Biochim Biophys Acta 742:558\u2013564","journal-title":"Biochim Biophys Acta"},{"key":"12808_CR17","doi-asserted-by":"publisher","first-page":"D265","DOI":"10.1093\/nar\/gkz991","volume":"48","author":"S Lu","year":"2020","unstructured":"Lu S, Wang J, Chitsaz F, Derbyshire MK, Geer RC, Gonzales NR, Gwadz M, Hurwitz DI, Marchler GH, Song JS, Thanki N, Yamashita RA, Yang M, Zhang D, Zheng C, Lanczycki CJ, Marchler-Bauer A (2020) CDD\/SPARCLE: the conserved domain database in 2020. Nucleic Acids Res 48:D265\u2013D268.-d268. https:\/\/doi.org\/10.1093\/nar\/gkz991","journal-title":"Nucleic Acids Res"},{"key":"12808_CR18","doi-asserted-by":"publisher","first-page":"e2006607","DOI":"10.1371\/journal.pbio.2006607","volume":"16","author":"T Maloney","year":"2018","unstructured":"Maloney T, Phelan R, Simmons N (2018) Saving the horseshoe crab: a synthetic alternative to horseshoe crab blood for endotoxin detection. PLOS Biol 16:e2006607. https:\/\/doi.org\/10.1371\/journal.pbio.2006607","journal-title":"PLOS Biol"},{"issue":"Web Server Issu","key":"12808_CR19","doi-asserted-by":"publisher","first-page":"W327","DOI":"10.1093\/nar\/gkh454","volume":"32","author":"A Marchler-Bauer","year":"2004","unstructured":"Marchler-Bauer A, Bryant SH (2004) CD-Search: protein domain annotations on the fly. Nucleic Acids Res 32(Web Server Issue):W327\u2013W331. https:\/\/doi.org\/10.1093\/nar\/gkh454","journal-title":"Nucleic Acids Res"},{"key":"12808_CR20","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1177\/1753425916681074","volume":"23","author":"H Mizumura","year":"2017","unstructured":"Mizumura H, Ogura N, Aketagawa J, Aizawa M, Kobayashi Y, Kawabata SI, Oda T (2017) Genetic engineering approach to develop next-generation reagents for endotoxin quantification. Innate Immun 23:136\u2013146. https:\/\/doi.org\/10.1177\/1753425916681074","journal-title":"Innate Immun"},{"key":"12808_CR21","first-page":"553","volume":"35","author":"T Mori","year":"1997","unstructured":"Mori T, Ikemoto H, Matsumura M, Yoshida M, Inada K, Endo S, Ito A, Watanabe S, Yamaguchi H, Mitsuya M, Kodama M, Tani T, Yokota T, Kobayashi T, Kambayashi J, Nakamura T, Masaoka T, Teshima H, Yoshinaga T et al (1997) Evaluation of plasma (1\u21923)-beta-D-glucan measurement by the kinetic turbidimetric Limulus test, for the clinical diagnosis of mycotic infections. Eur J Clin Chem Clin Biochem 35:553\u2013560","journal-title":"Eur J Clin Chem Clin Biochem"},{"key":"12808_CR22","doi-asserted-by":"publisher","first-page":"4155","DOI":"10.2174\/138161206778743529","volume":"12","author":"T Muta","year":"2006","unstructured":"Muta T (2006) Molecular basis for invertebrate innate immune recognition of (1\u21923)-beta-D-glucan as a pathogen-associated molecular pattern. Curr Pharm Des 12:4155\u20134161. https:\/\/doi.org\/10.2174\/138161206778743529","journal-title":"Curr Pharm Des"},{"key":"12808_CR23","doi-asserted-by":"publisher","first-page":"892","DOI":"10.1074\/jbc.270.2.892","volume":"270","author":"T Muta","year":"1995","unstructured":"Muta T, Seki N, Takaki Y, Hashimoto R, Oda T, Iwanaga A, Tokunaga F, Iwanaga S (1995) Purified horseshoe crab Factor G. Reconstitution and characterization of the (1\u21923)-beta-D-glucan-sensitive serine protease cascade. J Biol Chem 270:892\u2013897. https:\/\/doi.org\/10.1074\/jbc.270.2.892","journal-title":"J Biol Chem"},{"key":"12808_CR24","doi-asserted-by":"publisher","first-page":"J141","DOI":"10.3314\/mmj.17.020","volume":"58","author":"T Obayashi","year":"2017","unstructured":"Obayashi T (2017) The plasma (1\u21923)-\u03b2-D-glucan assay, a Japanese contribution to the diagnosis of invasive fungal infection. Med Mycol J 58:J141\u2013J147. https:\/\/doi.org\/10.3314\/mmj.17.020","journal-title":"Med Mycol J"},{"key":"12808_CR25","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/S0140-6736(95)91152-9","volume":"345","author":"T Obayashi","year":"1995","unstructured":"Obayashi T, Kawai T, Yoshida M, Mori T, Goto H, Yasuoka A, Shimada K, Iwasaki H, Teshima H, Kohno S, Horiuchi A, Ito A, Yamaguchi H (1995) Plasma (1\u21923)-\u03b2-D-glucan measurement in diagnosis of invasive deep mycosis and fungal febrile episodes. Lancet 345:17\u201320. https:\/\/doi.org\/10.1016\/S0140-6736(95)91152-9","journal-title":"Lancet"},{"key":"12808_CR26","doi-asserted-by":"publisher","first-page":"1864","DOI":"10.1086\/588295","volume":"46","author":"T Obayashi","year":"2008","unstructured":"Obayashi T, Negishi K, Suzuki T, Funata N (2008) Reappraisal of the serum (1\u21923)-\u03b2-D-glucan assay for the diagnosis of invasive fungal infections\u2014a study based on autopsy cases from 6 years. Clin Infect Dis 46:1864\u20131870. https:\/\/doi.org\/10.1086\/588295","journal-title":"Clin Infect Dis"},{"key":"12808_CR27","first-page":"4","volume":"19","author":"T Sandle","year":"2013","unstructured":"Sandle T (2013) Variability and the LAL assay. Pharm Microbiol Forum Newsletter 19:4\u201312","journal-title":"Pharm Microbiol Forum Newsletter"},{"key":"12808_CR28","doi-asserted-by":"publisher","first-page":"1370","DOI":"10.1016\/S0021-9258(17)42267-8","volume":"269","author":"N Seki","year":"1994","unstructured":"Seki N, Muta T, Oda T, Iwaki D, Kuma K, Miyata T, Iwanaga S (1994) Horseshoe crab (1,3)-beta-D-glucan-sensitive coagulation factor G. A serine protease zymogen heterodimer with similarities to beta-glucan-binding proteins. J Biol Chem 269:1370\u20131374","journal-title":"J Biol Chem"},{"key":"12808_CR29","doi-asserted-by":"publisher","first-page":"11589","DOI":"10.1074\/jbc.ra118.002311","volume":"293","author":"T Shibata","year":"2018","unstructured":"Shibata T, Kobayashi Y, Ikeda Y, Kawabata SI (2018) Intermolecular autocatalytic activation of serine protease zymogen factor C through an active transition state responding to lipopolysaccharide. J Biol Chem 293:11589\u201311599. https:\/\/doi.org\/10.1074\/jbc.ra118.002311","journal-title":"J Biol Chem"},{"key":"12808_CR30","doi-asserted-by":"publisher","first-page":"14281","DOI":"10.1074\/jbc.m200177200","volume":"277","author":"Y Takaki","year":"2002","unstructured":"Takaki Y, Seki N, Kawabata Si S-I, Iwanaga S, Muta T (2002) Duplicated binding sites for (1\u21923)-\u03b2-d-glucan in the horseshoe crab coagulation factor G. J Biol Chem 277:14281\u201314287. https:\/\/doi.org\/10.1074\/jbc.m200177200","journal-title":"J Biol Chem"},{"key":"12808_CR31","doi-asserted-by":"publisher","first-page":"3810","DOI":"10.4049\/jimmunol.0900430","volume":"183","author":"Y Ueda","year":"2009","unstructured":"Ueda Y, Ohwada S, Abe Y, Shibata T, Iijima M, Yoshimitsu Y, Koshiba T, Nakata M, Ueda T, Kawabata S-I (2009) Factor G utilizes a carbohydrate-binding cleft that is conserved between horseshoe crab and bacteria for the recognition of \u03b2-1,3-d-glucans. J Immunol 183:3810\u20133818. https:\/\/doi.org\/10.4049\/jimmunol.0900430","journal-title":"J Immunol"},{"key":"12808_CR32","doi-asserted-by":"publisher","first-page":"432","DOI":"10.1016\/j.jbiosc.2022.07.010","volume":"134","author":"Y Yamamoto","year":"2022","unstructured":"Yamamoto Y, Kajiura H, Nishibu T, Fujiyama K (2022) Mamestra brassicae NIAS-Mb-32 cell strain 2g2 enables high-yield recombinant protein production in baculovirus-free and baculovirus-based insect cell expression. J Biosci Bioeng 134:432\u2013440. https:\/\/doi.org\/10.1016\/j.jbiosc.2022.07.010","journal-title":"J Biosci Bioeng"},{"key":"12808_CR33","doi-asserted-by":"publisher","first-page":"8857","DOI":"10.1074\/jbc.ra119.012452","volume":"295","author":"K Yamashita","year":"2020","unstructured":"Yamashita K, Shibata T, Takahashi T, Kobayashi Y, Kawabata SI (2020) Roles of the clip domains of two protease zymogens in the coagulation cascade in horseshoe crabs. J Biol Chem 295:8857\u20138866. https:\/\/doi.org\/10.1074\/jbc.ra119.012452","journal-title":"J Biol Chem"},{"key":"12808_CR34","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1016\/j.jim.2010.12.011","volume":"365","author":"A Yoneda","year":"2011","unstructured":"Yoneda A, Kurokawa T (2011) A sensitive sandwich ELISA to measure (1\u21923)-\u03b2-d-glucan levels in blood. J Immunol Methods 365:158\u2013165. https:\/\/doi.org\/10.1016\/j.jim.2010.12.011","journal-title":"J Immunol Methods"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-023-12808-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00253-023-12808-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-023-12808-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T10:05:49Z","timestamp":1700215549000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00253-023-12808-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,26]]},"references-count":34,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["12808"],"URL":"https:\/\/doi.org\/10.1007\/s00253-023-12808-6","relation":{},"ISSN":["0175-7598","1432-0614"],"issn-type":[{"value":"0175-7598","type":"print"},{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,26]]},"assertion":[{"value":"24 May 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 August 2023","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 September 2023","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 October 2023","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This article does not contain any studies with human participants or animals performed by any of the authors.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical statement"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}