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The pig industry has suffered tremendous financial losses due to the high mortality rate of piglets caused by PEDV. Consequently, a simple and rapid on-site diagnostic technology is crucial for preventing and controlling PEDV. This study established a detection method for PEDV using recombinase-aided amplification (RAA) and <jats:italic>Pyrococcus furiosus<\/jats:italic> Argonaute (PfAgo), which can detect 100 copies of PEDV without cross-reactivity with other pathogens. The entire reaction of RAA and PfAgo to detect PEDV does not require sophisticated instruments, and the reaction results can be observed with the naked eye. Overall, this integrated RAA-PfAgo cleavage assay is a practical tool for accurately and quickly detecting PEDV.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Key points<\/jats:title>\n            <jats:p>\n              <jats:italic>\u2022 PfAgo has the potential to serve as a viable molecular diagnostic tool for the detection and diagnosis of viral genomes<\/jats:italic>\n            <\/jats:p>\n            <jats:p>\n              <jats:italic>\u2022 The RAA-PfAgo detection technique has a remarkable level of sensitivity and specificity<\/jats:italic>\n            <\/jats:p>\n            <jats:p>\n              <jats:italic>\u2022 The RAA-PfAgo detection system can identify PEDV without needing advanced equipment<\/jats:italic>\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s00253-023-12919-0","type":"journal-article","created":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T11:02:50Z","timestamp":1705316570000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Pyrococcus furiosus Argonaute-mediated porcine epidemic diarrhea virus detection"],"prefix":"10.1007","volume":"108","author":[{"given":"Yu","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changyu","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boyan","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7244-9929","authenticated-orcid":false,"given":"Hongning","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,1,15]]},"reference":[{"key":"12919_CR1","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1016\/S0034-5288(18)32994-1","volume":"25","author":"D Chasey","year":"1978","unstructured":"Chasey D, Cartwright SF (1978) Virus-like particles associated with porcine epidemic diarrhoea. 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