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Here, we created the genetic constructions, based on genome elements of potato virus X (PVX), carrying the <jats:italic>inf\u03b12b<\/jats:italic> gene for transient expression in plant cells. The created plasmid vector constructions were tested through <jats:italic>Agrobacterium<\/jats:italic>-mediated transient gene expression method in two plant species\u2014<jats:italic>Nicotiana benthamiana<\/jats:italic> and <jats:italic>Ocimum basilicum<\/jats:italic> (sweet basil). Production of recombinant hINF alpha 2b was more efficient in <jats:italic>N. benthamiana<\/jats:italic> than that in <jats:italic>O. basilicum<\/jats:italic> plants. The average yield of hINF alpha 2b produced in <jats:italic>N. benthamiana<\/jats:italic> plants was 0.56\u00a0mg\/g of fresh leaf weight (FW) or 6% of the total soluble cell proteins (TSP). The maximal level reached up to 1.2\u00a0mg\/g FW or 9% TSP. We estimated that about 0.67\u00a0mg of hINF can be obtained from one <jats:italic>N. benthamiana<\/jats:italic> plant. The yield of hINF alpha 2b obtained with the PVX-based expression cassette was about 80 times higher than the yield of hINF alpha 2b obtained with a simple expression cassette in which the <jats:italic>inf\u03b12b<\/jats:italic> gene was controlled by the 35S promoter of cauliflower mosaic virus.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Key points<\/jats:title>\n            <jats:p>\n              <jats:bold>\n                <jats:italic>\u2022<\/jats:italic>\n              <\/jats:bold>\n              <jats:italic> PVX-based expression vectors provide efficient transient expression of inf\u03b12b gene<\/jats:italic>\n            <\/jats:p>\n            <jats:p>\n              <jats:bold>\n                <jats:italic>\u2022<\/jats:italic>\n              <\/jats:bold>\n              <jats:italic> N. benthamiana plants can produce human interferon alpha 2b at high levels<\/jats:italic>\n            <\/jats:p>\n            <jats:p>\n              <jats:bold>\n                <jats:italic>\u2022<\/jats:italic>\n              <\/jats:bold>\n              <jats:italic> The yield of the hINF \u03b12b reached up to 1.2\u00a0mg\/g of fresh leaf weight<\/jats:italic>\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s00253-024-13069-7","type":"journal-article","created":{"date-parts":[[2024,2,23]],"date-time":"2024-02-23T10:02:39Z","timestamp":1708682559000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Construction of viral-based expression vectors for high-level production of human interferon alpha 2b in plants"],"prefix":"10.1007","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3098-5085","authenticated-orcid":false,"given":"Yana","family":"Sindarovska","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7365-7474","authenticated-orcid":false,"given":"Mykola","family":"Kuchuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,2,23]]},"reference":[{"issue":"4","key":"13069_CR1","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1111\/j.1467-7652.2007.00258.x","volume":"5","author":"PA Arlen","year":"2007","unstructured":"Arlen PA, Falconer R, Cherukumilli S, Cole A, Cole AM, Oishi KK, Daniell H (2007) Field production and functional evaluation of chloroplast-derived interferon-alpha2b. 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