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Vaccination stands out as one of the most effective preventive measures for susceptible populations. Within the outer membrane of <jats:italic>Leptospira<\/jats:italic> spp., we find the LIC12287, LIC11711, and LIC13259 lipoproteins. These are of interest due to their surface location and potential immunogenicity. Thorough examination revealed the conservation of these proteins among pathogenic <jats:italic>Leptospira<\/jats:italic> spp.; we mapped the distribution of T- and B-cell epitopes along their sequences and assessed the 3D structures of each protein. This information aided in selecting immunodominant regions for the development of a chimeric protein. Through gene synthesis, we successfully constructed a chimeric protein, which was subsequently expressed, purified, and characterized. Hamsters were immunized with the chimeric lipoprotein, formulated with adjuvants aluminum hydroxide, EMULSIGEN\u00ae-D, Sigma Adjuvant System\u00ae, and Montanide\u2122 ISA206VG. Another group was vaccinated with an inactivated <jats:italic>Escherichia coli<\/jats:italic> bacterin expressing the chimeric protein. Following vaccination, hamsters were challenged with a virulent <jats:italic>L. interrogans<\/jats:italic> strain. Our evaluation of the humoral immune response revealed the production of IgG antibodies, detectable 28\u00a0days after the second dose, in contrast to pre-immune samples and control groups. This demonstrates the potential of the chimeric protein to elicit a robust humoral immune response; however, no protection against challenge was achieved. While this study provides valuable insights into the subject, further research is warranted to identify protective antigens that could be utilized in the development of a leptospirosis vaccine.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Key points<\/jats:title>\n            <jats:p>\u2022 <jats:italic>Several T- and B-cell epitopes were identified in all the three proteins.<\/jats:italic>\n            <\/jats:p>\n            <jats:p>\u2022 <jats:italic>Four different adjuvants were used in vaccine formulations.<\/jats:italic>\n            <\/jats:p>\n            <jats:p>\u2022 <jats:italic>Immunization stimulated significant levels of IgG2\/3 in vaccinated animals.<\/jats:italic>\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s00253-024-13196-1","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T12:01:45Z","timestamp":1721649705000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Chimeric lipoproteins for leptospirosis vaccine: immunogenicity and protective potential"],"prefix":"10.1007","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2974-3057","authenticated-orcid":false,"given":"Rafael Carracena de Souza","family":"Tapaj\u00f3z","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9145-7123","authenticated-orcid":false,"given":"Francisco Denis Souza","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2230-6890","authenticated-orcid":false,"given":"Natasha Rodrigues","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1926-3260","authenticated-orcid":false,"given":"Mara Andrade Colares","family":"Maia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2003-4980","authenticated-orcid":false,"given":"Amilton Clair Pinto","family":"Seixas Neto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1456-2761","authenticated-orcid":false,"given":"Laura de Vargas","family":"Maiocchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7283-1561","authenticated-orcid":false,"given":"Pedro Henrique Filgueiras Coelho","family":"Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5330-1462","authenticated-orcid":false,"given":"Tha\u00eds Larr\u00e9","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2803-4088","authenticated-orcid":false,"given":"Odir Ant\u00f4nio","family":"Dellagostin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,7,22]]},"reference":[{"key":"13196_CR1","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1007\/978-3-662-45059-8_10","volume":"387","author":"B Adler","year":"2015","unstructured":"Adler B (2015) Vaccines against leptospirosis. 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