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Acetate production from CO<jats:sub>2<\/jats:sub> in microbial electrosynthesis (MES) has been widely studied, while more valuable compounds such as butyrate are currently attracting attention. In this study, methanol was used as a co-substrate with CO<jats:sub>2<\/jats:sub> to enhance butyrate production in MES. Feeding with CO<jats:sub>2<\/jats:sub> and methanol resulted in the highest butyrate production rates and titres of 0.36\u2009\u00b1\u20090.01\u00a0g L<jats:sup>\u22121<\/jats:sup> d<jats:sup>\u22121<\/jats:sup> and 8.6\u2009\u00b1\u20090.2\u00a0g L<jats:sup>\u22121<\/jats:sup>, respectively, outperforming reactors with only CO<jats:sub>2<\/jats:sub> feeding (0.20\u2009\u00b1\u20090.03\u00a0g L<jats:sup>\u22121<\/jats:sup> d<jats:sup>\u22121<\/jats:sup> and 5.2\u2009\u00b1\u20090.1\u00a0g L<jats:sup>\u22121<\/jats:sup>, respectively). Methanol acted as electron donor and as carbon source, both of which contributed ca. 50% of the carbon in the products. <jats:italic>Eubacterium<\/jats:italic> was the dominant genus with 52.6\u2009\u00b1\u20092.5% relative abundance. Thus, we demonstrate attractive route for the use of the C1 substrates, CO<jats:sub>2<\/jats:sub> and methanol, to produce mainly butyrate.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Key points<\/jats:title>\n            <jats:p>\u2022 <jats:italic>Butyrate was the main product from methanol and CO<\/jats:italic>\n              <jats:sub>\n                <jats:italic>2<\/jats:italic>\n              <\/jats:sub>\n              <jats:italic>in MES<\/jats:italic>\n            <\/jats:p>\n            <jats:p>\u2022 <jats:italic>Methanol acted as both carbon and electron source in MES<\/jats:italic>\n            <\/jats:p>\n            <jats:p>\u2022 <jats:italic>Eubacterium dominating microbial culture was enriched in MES<\/jats:italic>\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Graphical Abstract<\/jats:title>\n          <\/jats:sec>","DOI":"10.1007\/s00253-024-13218-y","type":"journal-article","created":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T06:02:20Z","timestamp":1718344940000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Methanol as a co-substrate with CO2 enhances butyrate production in microbial electrosynthesis"],"prefix":"10.1007","volume":"108","author":[{"given":"Hui","family":"Yao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna M.","family":"Rinta-Kanto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Vassilev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2208-8803","authenticated-orcid":false,"given":"Marika","family":"Kokko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,6,14]]},"reference":[{"key":"13218_CR1","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1111\/j.1442-9993.2001.01070.pp.x","volume":"26","author":"MJ Anderson","year":"2008","unstructured":"Anderson MJ (2008) A new method for non-parametric multivariate analysis of variance. 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