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For this purpose, phenylalanine dehydrogenase from\n                      <jats:italic>Bacillus sphaericus<\/jats:italic>\n                      was combined with the novel hydroxyphenylpyruvate reductase from\n                      <jats:italic>Mentha x piperita<\/jats:italic>\n                      , thereby providing an in situ cofactor regeneration throughout the conversion process. The purified enzymes were co-immobilized and subsequently employed in batch biotransformation, resulting in 60% conversion of 10\u00a0mM L-dopa within 24\u00a0h, with a catalytic amount of NAD\n                      <jats:sup>+<\/jats:sup>\n                      as cofactor. Furthermore, the bienzymatic system was implemented as a packed-bed reactor in continuous flow, achieving a conversion rate up to 80% with 60\u00a0min retention time. The process was further intensified by implementing a 48-h flow bioreaction. The biocatalysts demonstrated remarkable stability, retaining 62% of their initial activity at the end of the process. The final productivity of the isolated compound (96% purity) was calculated to be 1.84\u00a0g L\n                      <jats:sup>\u22121<\/jats:sup>\n                      \u00a0h\n                      <jats:sup>\u22121<\/jats:sup>\n                      yielding a sustainable synthesis of danshensu.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      <jats:italic>\u2022 Characterization of the hydroxyphenylpyruvate reductase from Mentha x piperita<\/jats:italic>\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:italic>\u2022 Bi-enzymatic system in a cascade reaction to produce danshensu<\/jats:italic>\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:italic>\u2022 Purification and isolation<\/jats:italic>\n                      <jats:italic>of the active compound danshensu<\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Graphical Abstract<\/jats:title>\n                  <\/jats:sec>","DOI":"10.1007\/s00253-025-13407-3","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T03:53:04Z","timestamp":1737431584000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Self-sufficient biocatalytic cascade for the continuous synthesis of danshensu in flow"],"prefix":"10.1007","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0294-166X","authenticated-orcid":false,"given":"Valentina","family":"Marchini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1704-0642","authenticated-orcid":false,"given":"Francesca","family":"Paradisi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,1,21]]},"reference":[{"key":"13407_CR1","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","volume":"215","author":"SF Altschul","year":"1990","unstructured":"Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. 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