{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:35:43Z","timestamp":1788568543596,"version":"build-2803163510"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,3,22]],"date-time":"2025-03-22T00:00:00Z","timestamp":1742601600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,3,22]],"date-time":"2025-03-22T00:00:00Z","timestamp":1742601600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"DOI":"10.1007\/s00253-025-13452-y","type":"journal-article","created":{"date-parts":[[2025,3,22]],"date-time":"2025-03-22T22:43:02Z","timestamp":1742683382000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Dual one-step recombinase-aided PCR for rapid detection of Candida in blood"],"prefix":"10.1007","volume":"109","author":[{"given":"Xiaona","family":"Lyu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenan","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiang","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijue","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanqing","family":"Tie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxin","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5891-5260","authenticated-orcid":false,"given":"Xuejun","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhishan","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,3,22]]},"reference":[{"key":"13452_CR1","doi-asserted-by":"publisher","first-page":"1775","DOI":"10.1128\/AEM.69.3.1775-1782.2003","volume":"69","author":"NE Brinkman","year":"2003","unstructured":"Brinkman NE, Haugland RA, Wymer LJ, Byappanahalli M, Whitman RL, Vesper SJ (2003) Evaluation of a rapid, quantitative real-time PCR method for enumeration of pathogenic Candida cells in water. Appl Environ Microbiol 69:1775\u20131782. https:\/\/doi.org\/10.1128\/AEM.69.3.1775-1782.2003","journal-title":"Appl Environ Microbiol"},{"key":"13452_CR2","doi-asserted-by":"publisher","unstructured":"Cafardi J, Haas D, Lamarre T, Feinberg J (2021) Opportunistic fungal infection associated with COVID-19. Open Forum Infect Dis 8:ofab016. https:\/\/doi.org\/10.1093\/ofid\/ofab016","DOI":"10.1093\/ofid\/ofab016"},{"key":"13452_CR3","doi-asserted-by":"publisher","unstructured":"Cao C, Bing J, Liao G, Nobile CJ, Huang G (2023) Candida haemulonii species complex: emerging fungal pathogens of the metschnikowiaceae clade. Zoonoses (Burlington) 3:43. https:\/\/doi.org\/10.15212\/zoonoses-2023-0021","DOI":"10.15212\/zoonoses-2023-0021"},{"key":"13452_CR25","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1016\/j.diagmicrobio.2017.10.005","volume":"90","author":"C Chen","year":"2018","unstructured":"Chen C, Li X, Li G, Zhao L, Duan S, Yan T, Feng Z, Ma X (2018) Use of a rapid reverse-transcription recombinase aided amplification assay for respiratory syncytial virus detection. Diagn Microbiol Infect Dis 90:90\u201395. https:\/\/doi.org\/10.1016\/j.diagmicrobio.2017.10.005","journal-title":"Diagn Microbiol Infect Dis"},{"key":"13452_CR4","doi-asserted-by":"publisher","first-page":"e00057","DOI":"10.1128\/JCM.00057-20","volume":"58","author":"X Chen","year":"2020","unstructured":"Chen X, Zheng H, Li W, Zhong Y, Chen X, Lu J (2020) Direct blood culturing of Candida spp. On solid medium by a rapid enrichment method with magnetic beads coated with recombinant human mannan-binding lectin. J Clin Microbiol 58:e00057\u2013e120. https:\/\/doi.org\/10.1128\/JCM.00057-20","journal-title":"J Clin Microbiol"},{"key":"13452_CR5","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.jbiotec.2016.12.022","volume":"243","author":"RH Deurenberg","year":"2017","unstructured":"Deurenberg RH, Bathoorn E, Chlebowicz MA, Couto N, Ferdous M, Garc\u00eda-Cobos S, Kooistra-Smid AMD, Raangs EC, Rosema S, Veloo ACM, Zhou K, Friedrich AW, Rossen JWA (2017) Application of next generation sequencing in clinical microbiology and infection prevention. J Biotechnol 243:16\u201324. https:\/\/doi.org\/10.1016\/j.jbiotec.2016.12.022","journal-title":"J Biotechnol"},{"key":"13452_CR6","doi-asserted-by":"publisher","first-page":"e24889","DOI":"10.1002\/jcla.24889","volume":"37","author":"G Fan","year":"2023","unstructured":"Fan G, He X, Zhang R, Tian F, Sun X, Zhang M, Li F, Shen X, Ma X (2023) A rapid and highly sensitive multiple detection of human adenovirus type 3, type 7 and respiratory syncytial virus by recombinase-aided reverse transcription PCR. J Clin Lab Anal 37:e24889. https:\/\/doi.org\/10.1002\/jcla.24889","journal-title":"J Clin Lab Anal"},{"key":"13452_CR7","doi-asserted-by":"publisher","first-page":"766411","DOI":"10.3389\/fbioe.2021.766411","volume":"9","author":"G Fan","year":"2021","unstructured":"Fan G, Zhang R, He X, Tian F, Nie M, Shen X, Ma X (2021) RAP: a novel approach to the rapid and highly sensitive detection of respiratory viruses. Front Bioeng Biotechnol 9:766411. https:\/\/doi.org\/10.3389\/fbioe.2021.766411","journal-title":"Front Bioeng Biotechnol"},{"key":"13452_CR8","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1186\/s12929-023-00926-2","volume":"30","author":"W Fang","year":"2023","unstructured":"Fang W, Wu J, Cheng M, Zhu X, Du M, Chen C, Liao W, Zhi K, Pan W (2023) Diagnosis of invasive fungal infections: challenges and recent developments. J Biomed Sci 30:42. https:\/\/doi.org\/10.1186\/s12929-023-00926-2","journal-title":"J Biomed Sci"},{"key":"13452_CR9","doi-asserted-by":"publisher","first-page":"374","DOI":"10.3390\/microorganisms10020374","volume":"10","author":"E Garc\u00eda-Salazar","year":"2022","unstructured":"Garc\u00eda-Salazar E, Acosta-Altamirano G, Betancourt-Cisneros P, Reyes-Montes MDR, Rosas-De-Paz E, Duarte-Escalante E, S\u00e1nchez-Conejo AR, Ocharan Hern\u00e1ndez E, Fr\u00edas-De-Le\u00f3n MG (2022) Detection and molecular identification of eight Candida species in clinical samples by simplex PCR. Microorganisms 10:374. https:\/\/doi.org\/10.3390\/microorganisms10020374","journal-title":"Microorganisms"},{"key":"13452_CR10","doi-asserted-by":"publisher","first-page":"520","DOI":"10.3390\/diagnostics14050520","volume":"14","author":"R Gudisa","year":"2024","unstructured":"Gudisa R, Harchand R, Rudramurthy SM (2024) Nucleic-acid-based molecular fungal diagnostics: a way to a better future. Diagnostics (Basel) 14:520. https:\/\/doi.org\/10.3390\/diagnostics14050520","journal-title":"Diagnostics (Basel)"},{"key":"13452_CR11","first-page":"397","volume":"45","author":"YKM Ismadi","year":"2023","unstructured":"Ismadi YKM, Mohamad S, Harun A (2023) Species-specific PCR primers for simultaneous detection of Aspergillus fumigatus, Aspergillus terreus, Candida albicans and Candida glabrata in invasive fungal infections. Malays J Pathol 45:397\u2013403","journal-title":"Malays J Pathol"},{"key":"13452_CR12","doi-asserted-by":"publisher","unstructured":"Lass-Fl\u00f6rl C, Steixner S (2023) The changing epidemiology of fungal infections. Mol Aspects Med 94:101240. https:\/\/doi.org\/10.1016\/j.mam.2023.101240","DOI":"10.1016\/j.mam.2023.101240"},{"key":"13452_CR13","doi-asserted-by":"publisher","first-page":"267","DOI":"10.3390\/jof7040267","volume":"7","author":"I Marekovi\u0107","year":"2021","unstructured":"Marekovi\u0107 I, Ple\u0161ko S, Rezo Vranje\u0161 V, Herljevi\u0107 Z, Kuli\u0161 T, Jandrli\u0107 M (2021) Epidemiology of candidemia: three-year results from a Croatian tertiary care hospital. J Fungi (Basel) 7:267. https:\/\/doi.org\/10.3390\/jof7040267","journal-title":"J Fungi (Basel)"},{"key":"13452_CR14","doi-asserted-by":"publisher","first-page":"612","DOI":"10.1111\/myc.13250","volume":"64","author":"JV Mulet Bayona","year":"2021","unstructured":"Mulet Bayona JV, Salvador Garc\u00eda C, Tormo Palop N, Gimeno Cardona C (2021) Validation and implementation of a commercial real-time PCR assay for direct detection of Candida auris from surveillance samples. Mycoses 64:612\u2013615. https:\/\/doi.org\/10.1111\/myc.13250","journal-title":"Mycoses"},{"key":"13452_CR15","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/j.cmi.2021.09.039","volume":"28","author":"AM Peri","year":"2022","unstructured":"Peri AM, Harris PNA, Paterson DL (2022) Culture-independent detection systems for bloodstream infection. Clin Microbiol Infect 28:195\u2013201. https:\/\/doi.org\/10.1016\/j.cmi.2021.09.039","journal-title":"Clin Microbiol Infect"},{"key":"13452_CR16","doi-asserted-by":"publisher","first-page":"2879","DOI":"10.1128\/JCM.00609-11","volume":"49","author":"CD Pfeiffer","year":"2011","unstructured":"Pfeiffer CD, Samsa GP, Schell WA, Reller LB, Perfect JR, Alexander BD (2011) Quantitation of Candida CFU in initial positive blood cultures. J Clin Microbiol 49:2879\u20132883. https:\/\/doi.org\/10.1128\/JCM.00609-11","journal-title":"J Clin Microbiol"},{"key":"13452_CR17","doi-asserted-by":"publisher","first-page":"511","DOI":"10.3390\/antibiotics11040511","volume":"11","author":"KC Tjandra","year":"2022","unstructured":"Tjandra KC, Ram-Mohan N, Abe R, Hashemi MM, Lee J, Chin SM, Roshardt MA, Liao JC, Wong PK, Yang S (2022) Diagnosis of bloodstream infections: an evolution of technologies towards accurate and rapid identification and antibiotic susceptibility testing. Antibiotics (Basel) 11:511. https:\/\/doi.org\/10.3390\/antibiotics11040511","journal-title":"Antibiotics (Basel)"},{"key":"13452_CR18","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1016\/j.mimet.2015.03.024","volume":"113","author":"A Vahidnia","year":"2015","unstructured":"Vahidnia A, Bekers W, Bliekendaal H, Spaargaren J (2015) High throughput multiplex-PCR for direct detection and diagnosis of dermatophyte species, Candida albicans and Candida parapsilosis in clinical specimen. J Microbiol Methods 113:38\u201340. https:\/\/doi.org\/10.1016\/j.mimet.2015.03.024","journal-title":"J Microbiol Methods"},{"key":"13452_CR19","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1007\/s00253-024-13019-3","volume":"108","author":"C Xiao-Ping","year":"2024","unstructured":"Xiao-Ping C, Hao Z, Ru-Li F, Jin-Xing L, Yu-Jun D, Ze-Yin L (2024) Recombinant mannan-binding lectin magnetic beads increase pathogen detection in immunocompromised patients. Appl Microbiol Biotechnol 108:193. https:\/\/doi.org\/10.1007\/s00253-024-13019-3","journal-title":"Appl Microbiol Biotechnol"},{"key":"13452_CR20","doi-asserted-by":"crossref","unstructured":"Zervou FN, Zacharioudakis IM, Kurpewski J, Mylonakis E (2017) T2 magnetic resonance for fungal diagnosis. Methods Mol Biol (Clifton, N.J.) 1508:305\u2013319","DOI":"10.1007\/978-1-4939-6515-1_18"},{"key":"13452_CR21","doi-asserted-by":"publisher","first-page":"823","DOI":"10.3760\/cma.j.cn112338-20230215-00079","volume":"44","author":"MY Zhang","year":"2023","unstructured":"Zhang MY, Chen XP, Sun XL, Ma XJ, Shen XX, Guo YY (2023) Establishment of a recombined mannose-binding lectin protein-magnetic beads-enriched binding recombinant enzyme-assisted polymerase chain reaction assay for Candida in blood samples. Zhonghua Liu Xing Bing Xue 44:823\u2013827. https:\/\/doi.org\/10.3760\/cma.j.cn112338-20230215-00079","journal-title":"Zhonghua Liu Xing Bing Xue"},{"key":"13452_CR22","doi-asserted-by":"publisher","first-page":"387","DOI":"10.3967\/bes2024.043","volume":"37","author":"ZJ Zhao","year":"2024","unstructured":"Zhao ZJ, Chen XP, Hua SW, Li FY, Zhao M, Xing CH, Wang J, Tian FY, Zhang RQ, Lyu XN, Han ZQ, Wang YX, Li HY, Shen XX, Ma XJ, Tie YQ (2024) Establishment of a multiplex detection method for common bacteria in blood based on human mannan-binding lectin protein-conjugated magnetic bead enrichment combined with recombinase-aided PCR technology. Biomedical and Environmental Sciences\u202f: BES 37:387\u2013398. https:\/\/doi.org\/10.3967\/bes2024.043","journal-title":"Biomed Environ Sci : BES"},{"key":"13452_CR23","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1007\/s00284-023-03318-9","volume":"80","author":"B Zhu","year":"2023","unstructured":"Zhu B, Wang L, Lu Y, Chen C, Wang K, Zhang L (2023) Recombinase polymerase amplification assay with lateral flow strips for rapid detection of candidiasis due to Candida parapsilosis. Curr Microbiol 80:217. https:\/\/doi.org\/10.1007\/s00284-023-03318-9","journal-title":"Curr Microbiol"},{"key":"13452_CR24","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1007\/s00284-023-03280-6","volume":"80","author":"H Zheng","year":"2023","unstructured":"Zheng H, Chen X, Li W, Lu J, Chen X (2023) Establishment of a fast diagnostic method for sepsis pathogens based on M1 bead enrichment. Curr Microbiol 80:166. https:\/\/doi.org\/10.1007\/s00284-023-03280-6","journal-title":"Curr Microbiol"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-025-13452-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00253-025-13452-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-025-13452-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T12:32:31Z","timestamp":1770294751000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00253-025-13452-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,22]]},"references-count":25,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["13452"],"URL":"https:\/\/doi.org\/10.1007\/s00253-025-13452-y","relation":{},"ISSN":["1432-0614"],"issn-type":[{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,22]]},"assertion":[{"value":"20 December 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 February 2025","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 March 2025","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 March 2025","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Ethical clearance for this study was granted by the Hebei General Hospital Ethics Committee (Research Ethics Committee approval, reference 2024-LW-0240). The study was conducted in accordance with the ethical guidelines and regulations. The samples used in this study were residual clinical specimens collected from previously approved projects after the completion of diagnostic testing. According to the guidelines established by the ethics committee, the requirement for additional informed consent was deemed unnecessary on the grounds that all patient identifiers had been meticulously removed, and the study did not impose any additional risks to the participants.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}],"article-number":"70"}}