{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T19:34:28Z","timestamp":1782934468261,"version":"3.54.5"},"reference-count":58,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,7,26]],"date-time":"2025-07-26T00:00:00Z","timestamp":1753488000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,7,26]],"date-time":"2025-07-26T00:00:00Z","timestamp":1753488000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","award":["137967"],"award-info":[{"award-number":["137967"]}],"id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","award":["137967"],"award-info":[{"award-number":["137967"]}],"id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003504","name":"University of the Western Cape","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003504","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      Efficient bioconversion of lignocellulosic biomass (LCB) to ethanol by\n                      <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                      requires its engineering to express heterologous enzymes at titres high enough to make significant impacts on industrial consolidated bioprocessing (CBP). Promoters are required for this purpose, but are reportedly influenced by various environmental factors as well as the protein specific nature of expression, warranting the need for assessment under the conditions for which they are intended. Heterologous xylosidase- and xylanase-encoding genes (\n                      <jats:italic>xln43_SED1<\/jats:italic>\n                      and\n                      <jats:italic>xyn2<\/jats:italic>\n                      ) were individually cloned under transcriptional control of the\n                      <jats:italic>SED1<\/jats:italic>\n                      <jats:sub>P<\/jats:sub>\n                      and\n                      <jats:italic>TDH3<\/jats:italic>\n                      <jats:sub>P<\/jats:sub>\n                      promoters, and\n                      <jats:italic>DIT1<\/jats:italic>\n                      <jats:sub>T<\/jats:sub>\n                      terminator, and integrated into the genome of an a\n                      <jats:italic>S.\u00a0cerevisiae<\/jats:italic>\n                      strain engineered for xylose utilization. Enzymatic assays were used to quantify the performance of the promoters when strains were cultivated on glucose (aerobically and micro-aerobically) and xylose. Additional strains containing both\n                      <jats:italic>xln43_SED1<\/jats:italic>\n                      and\n                      <jats:italic>xyn2<\/jats:italic>\n                      under different promoter combinations were then used to allow direct fermentation of beechwood xylan to ethanol in a CBP. The\n                      <jats:italic>SED1<\/jats:italic>\n                      <jats:sub>P<\/jats:sub>\n                      \/\n                      <jats:italic>DIT1<\/jats:italic>\n                      <jats:sub>T<\/jats:sub>\n                      and\n                      <jats:italic>TDH3<\/jats:italic>\n                      <jats:sub>P<\/jats:sub>\n                      \/\n                      <jats:italic>DIT1<\/jats:italic>\n                      <jats:sub>T<\/jats:sub>\n                      combinations significantly outperformed the benchmark\n                      <jats:italic>ENO1<\/jats:italic>\n                      <jats:sub>P\/T<\/jats:sub>\n                      under all of the tested cultivation conditions, as well as with regard to growth trials on non-native substrates (xylo-oligosaccharides\/XOS and beechwood xylan) and fermentations of beechwood xylan to ethanol. Overall,\n                      <jats:italic>TDH3<\/jats:italic>\n                      <jats:sub>P<\/jats:sub>\n                      was the best-performing promoter. This study demonstrates that heterologous metabolic pathways and CBP can be significantly enhanced by employing carefully selected promoters tailored to specific conditions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      \u2022\n                      <jats:italic>Promoters are unpredictable and must be tested under their intended conditions.<\/jats:italic>\n                    <\/jats:p>\n                    <jats:p>\n                      \u2022\n                      <jats:italic>TDH3<\/jats:italic>\n                      <jats:sub>\n                        <jats:italic>P<\/jats:italic>\n                      <\/jats:sub>\n                      ,\n                      <jats:italic>SED1<\/jats:italic>\n                      <jats:sub>\n                        <jats:italic>P<\/jats:italic>\n                      <\/jats:sub>\n                      ,\n                      <jats:italic>and DIT1<\/jats:italic>\n                      <jats:sub>\n                        <jats:italic>T<\/jats:italic>\n                      <\/jats:sub>\n                      <jats:italic>were effective in enhancing heterologous xylanase activity.<\/jats:italic>\n                    <\/jats:p>\n                    <jats:p>\n                      \u2022\n                      <jats:italic>Optimized xylanolytic enzyme expression improved CBP of xylan to ethanol.<\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00253-025-13563-6","type":"journal-article","created":{"date-parts":[[2025,7,26]],"date-time":"2025-07-26T13:33:30Z","timestamp":1753536810000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Identifying promoters to enhance heterologous gene expression in recombinant Saccharomyces cerevisiae strains cultivated on non-native substrates"],"prefix":"10.1007","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7528-806X","authenticated-orcid":false,"given":"Jordan","family":"Fortuin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6983-6226","authenticated-orcid":false,"given":"Riaan","family":"den Haan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,26]]},"reference":[{"issue":"3","key":"13563_CR1","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/0168-1656(92)90074-J","volume":"23","author":"MJ Bailey","year":"1992","unstructured":"Bailey MJ, Biely P, Poutanen K (1992) Interlaboratory testing of methods for assay of xylanase activity. J Biotechnol 23(3):257\u2013270. https:\/\/doi.org\/10.1016\/0168-1656(92)90074-J","journal-title":"J Biotechnol"},{"key":"13563_CR2","doi-asserted-by":"publisher","unstructured":"Balan V (2014) Current challenges in commercially producing biofuels from lignocellulosic biomass. Int Sch Res Not.\u00a0https:\/\/doi.org\/10.1155\/2014\/463074","DOI":"10.1155\/2014\/463074"},{"issue":"1","key":"13563_CR3","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1002\/bab.1819","volume":"67","author":"G Cedras","year":"2020","unstructured":"Cedras G, Kroukamp H, Van Zyl WH, Den Haan R (2020) The in vivo detection and measurement of the unfolded protein response in recombinant cellulase producing Saccharomyces cerevisiae strains. Biotechnol Appl Biochem 67(1):82\u201394. https:\/\/doi.org\/10.1002\/bab.1819","journal-title":"Biotechnol Appl Biochem"},{"key":"13563_CR4","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/j.ymben.2020.02.004","volume":"59","author":"A Claes","year":"2020","unstructured":"Claes A, Deparis Q, Foulqui\u00e9-Moreno MR, Thevelein JM (2020) Simultaneous secretion of seven lignocellulolytic enzymes by an industrial second-generation yeast strain enables efficient ethanol production from multiple polymeric substrates. Metab Eng 59:131\u2013141. https:\/\/doi.org\/10.1016\/j.ymben.2020.02.004","journal-title":"Metab Eng"},{"key":"13563_CR5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13068-019-1360-8","volume":"12","author":"JT Cunha","year":"2019","unstructured":"Cunha JT, Soares PO, Roman\u00ed A, Thevelein JM, Domingues L (2019) Xylose fermentation efficiency of industrial Saccharomyces cerevisiae yeast with separate or combined xylose reductase\/xylitol dehydrogenase and xylose isomerase pathways. Biotechnol Biofuels 12:1\u201314. https:\/\/doi.org\/10.1186\/s13068-019-1360-8","journal-title":"Biotechnol Biofuels"},{"key":"13563_CR6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13068-020-01780-2","volume":"13","author":"JT Cunha","year":"2020","unstructured":"Cunha JT, Roman\u00ed A, Inokuma K, Johansson B, Hasunuma T, Kondo A, Domingues L (2020) Consolidated bioprocessing of corn cob-derived hemicellulose: engineered industrial Saccharomyces cerevisiae as efficient whole cell biocatalysts. Biotechnol Biofuels 13:1\u201315. https:\/\/doi.org\/10.1186\/s13068-020-01780-2","journal-title":"Biotechnol Biofuels"},{"key":"13563_CR7","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2019.109382","volume":"131","author":"SA Davison","year":"2019","unstructured":"Davison SA, Keller NT, van Zyl WH, den Haan R (2019) Improved cellulase expression in diploid yeast strains enhanced consolidated bioprocessing of pretreated corn residues. Enzyme Microb Technol 131:109382. https:\/\/doi.org\/10.1016\/j.enzmictec.2019.109382","journal-title":"Enzyme Microb Technol"},{"issue":"6","key":"13563_CR8","doi-asserted-by":"publisher","first-page":"983","DOI":"10.1002\/jctb.4068","volume":"88","author":"R Den Haan","year":"2013","unstructured":"Den Haan R, Kroukamp H, Mert M, Bloom M, G\u00f6rgens JF, Van Zyl WH (2013) Engineering Saccharomyces cerevisiae for next generation ethanol production. J Chem Technol Biotechnol 88(6):983\u2013991. https:\/\/doi.org\/10.1002\/jctb.4068","journal-title":"J Chem Technol Biotechnol"},{"key":"13563_CR9","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2021.107859","volume":"53","author":"R Den Haan","year":"2021","unstructured":"Den Haan R, Rose SH, Cripwell RA, Trollope KM, Myburgh MW, Viljoen-Bloom M, van Zyl WH (2021) Heterologous production of cellulose-and starch-degrading hydrolases to expand Saccharomyces cerevisiae substrate utilization: lessons learnt. Biotechnol Adv 53:107859. https:\/\/doi.org\/10.1016\/j.biotechadv.2021.107859","journal-title":"Biotechnol Adv"},{"key":"13563_CR10","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1754-6834-6-168","volume":"6","author":"L Favaro","year":"2013","unstructured":"Favaro L, Basaglia M, Trento A, Van Rensburg E, Garc\u00eda-Aparicio M, Van Zyl WH, Casella S (2013) Exploring grape marc as trove for new thermotolerant and inhibitor-tolerant Saccharomyces cerevisiae strains for second-generation bioethanol production. Biotechnol Biofuels 6:1\u201314. https:\/\/doi.org\/10.1186\/1754-6834-6-168","journal-title":"Biotechnol Biofuels"},{"issue":"6","key":"13563_CR11","doi-asserted-by":"publisher","first-page":"800","DOI":"10.1080\/07388551.2019.1619157","volume":"39","author":"L Favaro","year":"2019","unstructured":"Favaro L, Jansen T, van Zyl WH (2019) Exploring industrial and natural Saccharomyces cerevisiae strains for the bio-based economy from biomass: the case of bioethanol. Crit Rev Biotechnol 39(6):800\u2013816. https:\/\/doi.org\/10.1080\/07388551.2019.1619157","journal-title":"Crit Rev Biotechnol"},{"issue":"7","key":"13563_CR12","doi-asserted-by":"publisher","first-page":"1795","DOI":"10.1039\/C8GC03864C","volume":"21","author":"G Guirimand","year":"2019","unstructured":"Guirimand G, Inokuma K, Bamba T, Matsuda M, Morita K, Sasaki K, Ogino C, Berrin J-G, Hasunuma T, Kondo A (2019) Cell-surface display technology and metabolic engineering of Saccharomyces cerevisiae for enhancing xylitol production from woody biomass. Green Chem 21(7):1795\u20131808. https:\/\/doi.org\/10.1039\/C8GC03864C","journal-title":"Green Chem"},{"key":"13563_CR13","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2020.105946","volume":"174","author":"J Guo","year":"2020","unstructured":"Guo J, Feng S, Cheng X (2020) Activity evaluation of glycolytic promoters from Escherichia coli and application for mevalonate biosynthesis. J Microbiol Methods 174:105946. https:\/\/doi.org\/10.1016\/j.mimet.2020.105946","journal-title":"J Microbiol Methods"},{"issue":"1","key":"13563_CR14","doi-asserted-by":"publisher","first-page":"kuac029","DOI":"10.1093\/jimb\/kuac029","volume":"50","author":"S He","year":"2023","unstructured":"He S, Zhang Z, Lu W (2023) Natural promoters and promoter engineering strategies for metabolic regulation in Saccharomyces cerevisiae. J Ind Microbiol Biotechnol 50(1):kuac029. https:\/\/doi.org\/10.1093\/jimb\/kuac029","journal-title":"J Ind Microbiol Biotechnol"},{"key":"13563_CR15","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2020.122740","volume":"300","author":"IM Hidayatullah","year":"2020","unstructured":"Hidayatullah IM, Setiadi T, Kresnowati MTAP, Boopathy R (2020) Xylanase inhibition by the derivatives of lignocellulosic material. Biores Technol 300:122740. https:\/\/doi.org\/10.1016\/j.biortech.2020.122740","journal-title":"Biores Technol"},{"key":"13563_CR16","doi-asserted-by":"publisher","unstructured":"Hubmann G, Thevelein JM, Nevoigt E (2014) Natural and modified promoters for tailored metabolic engineering of the yeast Saccharomyces cerevisiae. Yeast metabolic engineering: methods and protocols, pp 17\u201342.\u00a0https:\/\/doi.org\/10.1007\/978-1-4939-0563-8_2","DOI":"10.1007\/978-1-4939-0563-8_2"},{"issue":"1","key":"13563_CR17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1754-6834-7-8","volume":"7","author":"K Inokuma","year":"2014","unstructured":"Inokuma K, Hasunuma T, Kondo A (2014) Efficient yeast cell-surface display of exo-and endo-cellulase using the SED1 anchoring region and its original promoter. Biotechnol Biofuels 7(1):1\u201311. https:\/\/doi.org\/10.1186\/1754-6834-7-8","journal-title":"Biotechnol Biofuels"},{"issue":"4","key":"13563_CR18","doi-asserted-by":"publisher","first-page":"486","DOI":"10.1016\/j.jbiotec.2013.09.024","volume":"168","author":"Y Ito","year":"2013","unstructured":"Ito Y, Yamanishi M, Ikeuchi A, Imamura C, Tokuhiro K, Kitagawa T, Matsuyama T (2013) Characterization of five terminator regions that increase the protein yield of a transgene in Saccharomyces cerevisiae. J Biotechnol 168(4):486\u2013492. https:\/\/doi.org\/10.1016\/j.jbiotec.2013.09.024","journal-title":"J Biotechnol"},{"issue":"1","key":"13563_CR19","doi-asserted-by":"publisher","first-page":"36997","DOI":"10.1038\/srep36997","volume":"6","author":"Y Ito","year":"2016","unstructured":"Ito Y, Kitagawa T, Yamanishi M, Katahira S, Izawa S, Irie K, Furutani-Seiki M, Matsuyama T (2016) Enhancement of protein production via the strong DIT1 terminator and two RNA-binding proteins in Saccharomyces cerevisiae. Sci Rep 6(1):36997. https:\/\/doi.org\/10.1038\/srep36997","journal-title":"Sci Rep"},{"issue":"23","key":"13563_CR20","doi-asserted-by":"publisher","first-page":"12317","DOI":"10.3390\/app122312317","volume":"12","author":"O Jacob","year":"2022","unstructured":"Jacob O, van Lill GR, den Haan R (2022) CRISPR-based multi-gene integration strategies to create Saccharomyces cerevisiae strains for consolidated bioprocessing. Appl Sci 12(23):12317. https:\/\/doi.org\/10.3390\/app122312317","journal-title":"Appl Sci"},{"key":"13563_CR21","doi-asserted-by":"publisher","unstructured":"Jansen ML, Bracher JM, Papapetridis I, Verhoeven MD, de Bruijn H, de Waal PP, van Maris AJ, Klaassen P, Pronk JT (2017) Saccharomyces cerevisiae strains for second-generation ethanol production: from academic exploration to industrial implementation. FEMS Yeast Res 17(5).\u00a0https:\/\/doi.org\/10.1093\/femsyr\/fox044","DOI":"10.1093\/femsyr\/fox044"},{"issue":"1","key":"13563_CR22","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1002\/bbb.1824","volume":"12","author":"H Kroukamp","year":"2018","unstructured":"Kroukamp H, den Haan R, van Zyl JH, van Zyl WH (2018) Rational strain engineering interventions to enhance cellulase secretion by Saccharomyces cerevisiae. Biofuels, Bioprod Biorefin 12(1):108\u2013124. https:\/\/doi.org\/10.1002\/bbb.1824","journal-title":"Biofuels, Bioprod Biorefin"},{"issue":"5","key":"13563_CR23","doi-asserted-by":"publisher","first-page":"1099","DOI":"10.1002\/jctb.7044","volume":"97","author":"F Kruger","year":"2022","unstructured":"Kruger F, Den Haan R (2022) Surface tethered xylosidase activity improved xylan conversion in engineered strains of Saccharomyces cerevisiae. J Chem Technol Biotechnol 97(5):1099\u20131111. https:\/\/doi.org\/10.1002\/jctb.7044","journal-title":"J Chem Technol Biotechnol"},{"issue":"12","key":"13563_CR24","doi-asserted-by":"publisher","first-page":"5512","DOI":"10.1128\/AEM.67.12.5512-5519.2001","volume":"67","author":"DC La Grange","year":"2001","unstructured":"La Grange DC, Pretorius IS, Claeyssens M, Van Zyl WH (2001) Degradation of xylan to D-xylose by recombinant Saccharomyces cerevisiae coexpressing the Aspergillus niger \u03b2-xylosidase (xlnD) and the Trichoderma reesei xylanase II (xyn2) genes. Appl Environ Microbiol 67(12):5512\u20135519. https:\/\/doi.org\/10.1128\/AEM.67.12.5512-5519.2001","journal-title":"Appl Environ Microbiol"},{"key":"13563_CR25","doi-asserted-by":"publisher","first-page":"380","DOI":"10.1016\/j.biortech.2018.04.013","volume":"260","author":"S Lane","year":"2018","unstructured":"Lane S, Dong J, Jin Y-S (2018) Value-added biotransformation of cellulosic sugars by engineered Saccharomyces cerevisiae. Biores Technol 260:380\u2013394. https:\/\/doi.org\/10.1016\/j.biortech.2018.04.013","journal-title":"Biores Technol"},{"key":"13563_CR26","doi-asserted-by":"publisher","first-page":"364","DOI":"10.1016\/j.ces.2018.09.030","volume":"193","author":"X Li","year":"2019","unstructured":"Li X, Wang Z, Zhang G, Yi L (2019) Improving lycopene production in Saccharomyces cerevisiae through optimizing pathway and chassis metabolism. Chem Eng Sci 193:364\u2013369. https:\/\/doi.org\/10.1016\/j.ces.2018.09.030","journal-title":"Chem Eng Sci"},{"issue":"4","key":"13563_CR27","doi-asserted-by":"publisher","first-page":"1267","DOI":"10.18331\/BRJ2020.7.4.5","volume":"7","author":"Y Lugani","year":"2020","unstructured":"Lugani Y, Rai R, Prabhu AA, Maan P, Hans M, Kumar V, Kumar S, Chandel AK, Sengar R (2020) Recent advances in bioethanol production from lignocelluloses: a comprehensive review with a focus on enzyme engineering and designer biocatalysts. Biofuel Res J 7(4):1267\u20131295. https:\/\/doi.org\/10.18331\/BRJ2020.7.4.5","journal-title":"Biofuel Res J"},{"key":"13563_CR28","doi-asserted-by":"publisher","unstructured":"Mao J, Zhang H, Chen Y, Wei L, Liu J, Nielsen J, Chen Y, Xu N (2024) Relieving metabolic burden to improve robustness and bioproduction by industrial microorganisms. Biotechnol Adv :108401.\u00a0https:\/\/doi.org\/10.1016\/j.biotechadv.2024.108401","DOI":"10.1016\/j.biotechadv.2024.108401"},{"issue":"4","key":"13563_CR29","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1007\/s10295-015-1727-1","volume":"43","author":"MJ Mert","year":"2016","unstructured":"Mert MJ, La Grange DC, Rose SH, van Zyl WH (2016) Engineering of Saccharomyces cerevisiae to utilize xylan as a sole carbohydrate source by co-expression of an endoxylanase, xylosidase and a bacterial xylose isomerase. J Ind Microbiol Biotechnol 43(4):431\u2013440. https:\/\/doi.org\/10.1007\/s10295-015-1727-1","journal-title":"J Ind Microbiol Biotechnol"},{"issue":"10","key":"13563_CR30","doi-asserted-by":"publisher","first-page":"1459","DOI":"10.1007\/s10295-017-1969-1","volume":"44","author":"M Mert","year":"2017","unstructured":"Mert M, Rose S, La Grange D, Bamba T, Hasunuma T, Kondo A, van Zyl W (2017) Quantitative metabolomics of a xylose-utilizing Saccharomyces cerevisiae strain expressing the Bacteroides thetaiotaomicron xylose isomerase on glucose and xylose. J Ind Microbiol Biotechnol 44(10):1459\u20131470. https:\/\/doi.org\/10.1007\/s10295-017-1969-1","journal-title":"J Ind Microbiol Biotechnol"},{"issue":"2","key":"13563_CR31","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1016\/j.ymben.2012.01.006","volume":"14","author":"MD Mikkelsen","year":"2012","unstructured":"Mikkelsen MD, Buron LD, Salomonsen B, Olsen CE, Hansen BG, Mortensen UH, Halkier BA (2012) Microbial production of indolylglucosinolate through engineering of a multi-gene pathway in a versatile yeast expression platform. Metab Eng 14(2):104\u2013111","journal-title":"Metab Eng"},{"key":"13563_CR32","doi-asserted-by":"publisher","first-page":"5205","DOI":"10.1007\/s00253-016-7555-z","volume":"100","author":"L Moreira","year":"2016","unstructured":"Moreira L, Filho E (2016) Insights into the mechanism of enzymatic hydrolysis of xylan. Appl Microbiol Biotechnol 100:5205\u20135214. https:\/\/doi.org\/10.1007\/s00253-016-7555-z","journal-title":"Appl Microbiol Biotechnol"},{"issue":"3","key":"13563_CR33","doi-asserted-by":"publisher","first-page":"207","DOI":"10.3390\/ijms17030207","volume":"17","author":"DN Moys\u00e9s","year":"2016","unstructured":"Moys\u00e9s DN, Reis VCB, Almeida JRMd, Moraes LMPd, Torres FAG (2016) Xylose fermentation by Saccharomyces cerevisiae: challenges and prospects. Int J Mol Sci 17(3):207. https:\/\/doi.org\/10.3390\/ijms17030207","journal-title":"Int J Mol Sci"},{"key":"13563_CR34","doi-asserted-by":"publisher","DOI":"10.1016\/j.jclepro.2023.136815","volume":"402","author":"M Mujtaba","year":"2023","unstructured":"Mujtaba M, Fraceto LF, Fazeli M, Mukherjee S, Savassa SM, de Medeiros GA, Santo Pereira AdE, Mancini SD, Lipponen J, Vilaplana F (2023) Lignocellulosic biomass from agricultural waste to the circular economy: a review with focus on biofuels, biocomposites and bioplastics. J Clean Prod 402:136815. https:\/\/doi.org\/10.1016\/j.jclepro.2023.136815","journal-title":"J Clean Prod"},{"key":"13563_CR35","doi-asserted-by":"publisher","unstructured":"Myburgh MW, Rose SH, Viljoen-Bloom M (2020) Evaluating and engineering Saccharomyces cerevisiae promoters for increased amylase expression and bioethanol production from raw starch. FEMS Yeast Res 20(6).\u00a0https:\/\/doi.org\/10.1093\/femsyr\/foaa047","DOI":"10.1093\/femsyr\/foaa047"},{"key":"13563_CR36","doi-asserted-by":"crossref","unstructured":"Myburgh MW, Schwerdtfeger KS, Cripwell RA, van Zyl WH, Viljoen-Bloom M (2023) Promoters and introns as key drivers for enhanced gene expression in Saccharomyces cerevisiae. Advances in Applied Microbiology, vol 124. Elsevier, pp 1\u201329","DOI":"10.1016\/bs.aambs.2023.07.002"},{"issue":"1","key":"13563_CR37","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/j.jbiosc.2017.08.001","volume":"125","author":"Y Nambu-Nishida","year":"2018","unstructured":"Nambu-Nishida Y, Sakihama Y, Ishii J, Hasunuma T, Kondo A (2018) Selection of yeast Saccharomyces cerevisiae promoters available for xylose cultivation and fermentation. J Biosci Bioeng 125(1):76\u201386. https:\/\/doi.org\/10.1016\/j.jbiosc.2017.08.001","journal-title":"J Biosci Bioeng"},{"issue":"1","key":"13563_CR38","doi-asserted-by":"publisher","first-page":"foz089","DOI":"10.1093\/femsyr\/foz089","volume":"20","author":"EJ Oh","year":"2020","unstructured":"Oh EJ, Jin Y-S (2020) Engineering of Saccharomyces cerevisiae for efficient fermentation of cellulose. FEMS Yeast Res 20(1):foz089. https:\/\/doi.org\/10.1093\/femsyr\/foz089","journal-title":"FEMS Yeast Res"},{"issue":"11","key":"13563_CR39","doi-asserted-by":"publisher","first-page":"1834","DOI":"10.3390\/agronomy10111834","volume":"10","author":"E Olguin-Maciel","year":"2020","unstructured":"Olguin-Maciel E, Singh A, Chable-Villacis R, Tapia-Tussell R, Ruiz HA (2020) Consolidated bioprocessing, an innovative strategy towards sustainability for biofuels production from crop residues: an overview. Agronomy 10(11):1834. https:\/\/doi.org\/10.3390\/agronomy10111834","journal-title":"Agronomy"},{"issue":"11","key":"13563_CR40","doi-asserted-by":"publisher","first-page":"955","DOI":"10.1002\/yea.1806","volume":"27","author":"S Partow","year":"2010","unstructured":"Partow S, Siewers V, Bj\u00f8rn S, Nielsen J, Maury J (2010) Characterization of different promoters for designing a new expression vector in Saccharomyces cerevisiae. Yeast 27(11):955\u2013964. https:\/\/doi.org\/10.1002\/yea.1806","journal-title":"Yeast"},{"issue":"1","key":"13563_CR41","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12934-015-0278-5","volume":"14","author":"B Peng","year":"2015","unstructured":"Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE (2015) Controlling heterologous gene expression in yeast cell factories on different carbon substrates and across the diauxic shift: a comparison of yeast promoter activities. Microb Cell Fact 14(1):1\u201311. https:\/\/doi.org\/10.1186\/s12934-015-0278-5","journal-title":"Microb Cell Fact"},{"key":"13563_CR42","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.procbio.2023.12.024","volume":"138","author":"GR Sahana","year":"2024","unstructured":"Sahana GR, Balasubramanian B, Joseph KS, Pappuswamy M, Liu W-C, Meyyazhagan A, Kamyab H, Chelliapan S, Joseph BV (2024) A review on ethanol tolerance mechanisms in yeast: Current knowledge in biotechnological applications and future directions. Process Biochem 138:1\u201313. https:\/\/doi.org\/10.1016\/j.procbio.2023.12.024","journal-title":"Process Biochem"},{"issue":"4","key":"13563_CR43","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1016\/j.jbiotec.2011.06.025","volume":"158","author":"T Sakamoto","year":"2012","unstructured":"Sakamoto T, Hasunuma T, Hori Y, Yamada R, Kondo A (2012) Direct ethanol production from hemicellulosic materials of rice straw by use of an engineered yeast strain codisplaying three types of hemicellulolytic enzymes on the surface of xylose-utilizing Saccharomyces cerevisiae cells. J Biotechnol 158(4):203\u2013210. https:\/\/doi.org\/10.1016\/j.jbiotec.2011.06.025","journal-title":"J Biotechnol"},{"issue":"1","key":"13563_CR44","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/jof4010001","volume":"4","author":"AB Sanz","year":"2017","unstructured":"Sanz AB, Garc\u00eda R, Rodr\u00edguez-Pe\u00f1a JM, Arroyo J (2017) The CWI pathway: regulation of the transcriptional adaptive response to cell wall stress in yeast. Journal of Fungi 4(1):1. https:\/\/doi.org\/10.3390\/jof4010001","journal-title":"Journal of Fungi"},{"key":"13563_CR45","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2022.107925","volume":"56","author":"J Sharma","year":"2022","unstructured":"Sharma J, Kumar V, Prasad R, Gaur NA (2022) Engineering of Saccharomyces cerevisiae as a consolidated bioprocessing host to produce cellulosic ethanol: Recent advancements and current challenges. Biotechnol Adv 56:107925. https:\/\/doi.org\/10.1016\/j.biotechadv.2022.107925","journal-title":"Biotechnol Adv"},{"issue":"13","key":"13563_CR46","doi-asserted-by":"publisher","first-page":"3381","DOI":"10.1128\/jb.180.13.3381-3387.1998","volume":"180","author":"H Shimoi","year":"1998","unstructured":"Shimoi H, Kitagaki H, Ohmori H, Iimura Y, Ito K (1998) Sed1p is a major cell wall protein of Saccharomyces cerevisiae in the stationary phase and is involved in lytic enzyme resistance. J Bacteriol 180(13):3381\u20133387. https:\/\/doi.org\/10.1128\/jb.180.13.3381-3387.1998","journal-title":"J Bacteriol"},{"issue":"8","key":"13563_CR47","doi-asserted-by":"publisher","first-page":"2082","DOI":"10.1002\/bit.24481","volume":"109","author":"J Sun","year":"2012","unstructured":"Sun J, Shao Z, Zhao H, Nair N, Wen F, Xu JH, Zhao H (2012) Cloning and characterization of a panel of constitutive promoters for applications in pathway engineering in Saccharomyces cerevisiae. Biotechnol Bioeng 109(8):2082\u20132092. https:\/\/doi.org\/10.1002\/bit.24481","journal-title":"Biotechnol Bioeng"},{"issue":"8","key":"13563_CR48","doi-asserted-by":"publisher","first-page":"320","DOI":"10.3390\/metabo10080320","volume":"10","author":"H Tang","year":"2020","unstructured":"Tang H, Wu Y, Deng J, Chen N, Zheng Z, Wei Y, Luo X, Keasling JD (2020) Promoter architecture and promoter engineering in Saccharomyces cerevisiae. Metabolites 10(8):320. https:\/\/doi.org\/10.3390\/metabo10080320","journal-title":"Metabolites"},{"issue":"2","key":"13563_CR49","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1002\/jib.224","volume":"121","author":"K Thomson","year":"2015","unstructured":"Thomson K, Bhat A, Carvell J (2015) Comparison of a new digital imaging technique for yeast cell counting and viability assessments with traditional methods. J Inst Brew 121(2):231\u2013237. https:\/\/doi.org\/10.1002\/jib.224","journal-title":"J Inst Brew"},{"issue":"1","key":"13563_CR50","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1016\/0003-2697(75)90739-3","volume":"66","author":"R Thuring","year":"1975","unstructured":"Thuring R, Sanders J, Borst P (1975) A freeze-squeeze method for recovering long DNA from agarose gels. Anal Biochem 66(1):213\u2013220. https:\/\/doi.org\/10.1016\/0003-2697(75)90739-3","journal-title":"Anal Biochem"},{"issue":"6","key":"13563_CR51","doi-asserted-by":"publisher","first-page":"1781","DOI":"10.1042\/BST20190293","volume":"47","author":"M Valenzuela-Ortega","year":"2019","unstructured":"Valenzuela-Ortega M, French CE (2019) Engineering of industrially important microorganisms for assimilation of cellulosic biomass: towards consolidated bioprocessing. Biochem Soc Trans 47(6):1781\u20131794. https:\/\/doi.org\/10.1042\/BST20190293","journal-title":"Biochem Soc Trans"},{"issue":"6","key":"13563_CR52","doi-asserted-by":"publisher","first-page":"1458","DOI":"10.1016\/j.biotechadv.2012.03.002","volume":"30","author":"J Van Dyk","year":"2012","unstructured":"Van Dyk J, Pletschke B (2012) A review of lignocellulose bioconversion using enzymatic hydrolysis and synergistic cooperation between enzymes\u2014factors affecting enzymes, conversion and synergy. Biotechnol Adv 30(6):1458\u20131480. https:\/\/doi.org\/10.1016\/j.biotechadv.2012.03.002","journal-title":"Biotechnol Adv"},{"issue":"1","key":"13563_CR53","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1475-2859-13-5","volume":"13","author":"K Weinhandl","year":"2014","unstructured":"Weinhandl K, Winkler M, Glieder A, Camattari A (2014) Carbon source dependent promoters in yeasts. Microb Cell Fact 13(1):1\u201317. https:\/\/doi.org\/10.1186\/1475-2859-13-5","journal-title":"Microb Cell Fact"},{"issue":"1","key":"13563_CR54","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1080\/21655979.2019.1682213","volume":"10","author":"W Xiao","year":"2019","unstructured":"Xiao W, Li H, Xia W, Yang Y, Hu P, Zhou S, Hu Y, Liu X, Dai Y, Jiang Z (2019) Co-expression of cellulase and xylanase genes in Saccharomyces cerevisiae toward enhanced bioethanol production from corn stover. Bioengineered 10(1):513\u2013521. https:\/\/doi.org\/10.1080\/21655979.2019.1682213","journal-title":"Bioengineered"},{"issue":"1","key":"13563_CR55","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12934-018-0899-6","volume":"17","author":"L Xiong","year":"2018","unstructured":"Xiong L, Zeng Y, Tang R-Q, Alper HS, Bai F-W, Zhao X-Q (2018) Condition-specific promoter activities in Saccharomyces cerevisiae. Microb Cell Fact 17(1):1\u201315. https:\/\/doi.org\/10.1186\/s12934-018-0899-6","journal-title":"Microb Cell Fact"},{"key":"13563_CR56","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s11274-019-2606-0","volume":"35","author":"N Xu","year":"2019","unstructured":"Xu N, Wei L, Liu J (2019) Recent advances in the applications of promoter engineering for the optimization of metabolite biosynthesis. World J Microbiol Biotechnol 35:1\u201310. https:\/\/doi.org\/10.1007\/s11274-019-2606-0","journal-title":"World J Microbiol Biotechnol"},{"key":"13563_CR57","doi-asserted-by":"publisher","unstructured":"Yang S, Song L, Wang J, Zhao J, Tang H, Bao X (2023) Engineering Saccharomyces cerevisiae for efficient production of recombinant proteins. Eng Microbiol :100122.\u00a0https:\/\/doi.org\/10.1016\/j.engmic.2023.100122","DOI":"10.1016\/j.engmic.2023.100122"},{"issue":"18","key":"13563_CR58","doi-asserted-by":"publisher","first-page":"5169","DOI":"10.1021\/acs.jafc.9b00863","volume":"67","author":"Y Zhang","year":"2019","unstructured":"Zhang Y, Min Z, Qin Y, Ye D-Q, Song Y-Y, Liu Y-L (2019) Efficient Display of Aspergillus niger \u03b2-glucosidase on Saccharomyces cerevisiae cell wall for aroma enhancement in wine. J Agric Food Chem 67(18):5169\u20135176","journal-title":"J Agric Food Chem"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-025-13563-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00253-025-13563-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-025-13563-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T12:30:38Z","timestamp":1770294638000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00253-025-13563-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,26]]},"references-count":58,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["13563"],"URL":"https:\/\/doi.org\/10.1007\/s00253-025-13563-6","relation":{},"ISSN":["1432-0614"],"issn-type":[{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,26]]},"assertion":[{"value":"13 March 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 June 2025","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 July 2025","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 July 2025","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This article does not contain any studies with animals or human participants performed by any of the authors.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"173"}}