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Having previously established plasma-driven biocatalysis using recombinant unspecific peroxygenase from\n                    <jats:italic>Agrocybe aegerita<\/jats:italic>\n                    (r\n                    <jats:italic>Aae<\/jats:italic>\n                    UPO) to produce (\n                    <jats:italic>R<\/jats:italic>\n                    )-1-phenylethanol from ethylbenzene, we here employed CypC from\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    168 (synonyms: YbdT, P450BS\u03b2), an integral enzyme of surfactin and fengycin biosynthesis. CypC naturally hydroxylates medium and long-chain carboxylic acids. With short-chain carboxylic acids as decoy molecules, it also converts non-natural substrates such as ethylbenzene. We optimized production and heme loading of CypC and established guaiacol and ABTS-based reactions to assess compatibility of CypC with plasma-driven biocatalysis regarding temperature and H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    operating windows. With heptanoic acid as the decoy molecule and H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    from stock solution, guaiacol and ABTS conversion yielded 18.28 and 21.13 nmol product min\n                    <jats:sup>\u22121<\/jats:sup>\n                    nmol\n                    <jats:sup>\u22121<\/jats:sup>\n                    <jats:sub>CypC<\/jats:sub>\n                    , respectively. We then supplied H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    using a capillary plasma jet operated with 1280\u00a0ppm H\n                    <jats:sub>2<\/jats:sub>\n                    O in helium to convert ethylbenzene with immobilized CypC in a rotating bed reactor (5\u00a0ml reaction volume). After 120\u00a0min run time, a turnover number (TON) of 18.82\u00a0mol\n                    <jats:sub>\n                      (\n                      <jats:italic>R<\/jats:italic>\n                      )-1-PhOl\n                    <\/jats:sub>\n                    \u00a0mol\n                    <jats:sup>\u22121<\/jats:sup>\n                    <jats:sub>CypC<\/jats:sub>\n                    was reached, demonstrating that plasma-driven biocatalysis can be extended to H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    -dependent enzymes beyond r\n                    <jats:italic>Aae<\/jats:italic>\n                    UPO to expand the product range.\n                  <\/jats:p>","DOI":"10.1007\/s00253-025-13568-1","type":"journal-article","created":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T04:35:44Z","timestamp":1756787744000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The CYP152-family P450 enzyme CypC of Bacillus subtilis converts non-natural substrates in plasma-driven biocatalysis"],"prefix":"10.1007","volume":"109","author":[{"given":"Tim","family":"Dirks","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Klopsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davina","family":"Stoesser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie Desdemona","family":"Trenkle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulkadir","family":"Yayci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Sch\u00fcttler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Golda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Elisabeth","family":"Bandow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,9,2]]},"reference":[{"key":"13568_CR1","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1016\/0006-2952(82)90186-1","volume":"31","author":"HJ Ahr","year":"1982","unstructured":"Ahr HJ, King LJ, Nastainczyk W, Ullrich V (1982) The mechanism of reductive dehalogenation of halothane by liver cytochrome P450. 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