{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T01:17:29Z","timestamp":1770340649009,"version":"3.49.0"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T00:00:00Z","timestamp":1766102400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T00:00:00Z","timestamp":1766188800000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"Defense Strategic Environmental Research and Development Program","award":["ER-2716"],"award-info":[{"award-number":["ER-2716"]}]},{"name":"Defense Strategic Environmental Research and Development Program","award":["ER-2716"],"award-info":[{"award-number":["ER-2716"]}]},{"name":"Defense Strategic Environmental Research and Development Program","award":["ER-2716"],"award-info":[{"award-number":["ER-2716"]}]},{"name":"Defense Strategic Environmental Research and Development Program","award":["ER-2716"],"award-info":[{"award-number":["ER-2716"]}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["R01ES032707"],"award-info":[{"award-number":["R01ES032707"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["R01ES032707"],"award-info":[{"award-number":["R01ES032707"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"DOI":"10.1007\/s00253-025-13569-0","type":"journal-article","created":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T14:03:44Z","timestamp":1766153024000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Toluene-2-monooxygenase expression and trichloroethene oxidizing activity of Burkholderia vietnamiensis G4 grown on benzyl alcohol and benzyl esters"],"prefix":"10.1007","volume":"109","author":[{"given":"Alyssa M.","family":"Saito","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandra P.","family":"Oyarz\u00fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael R.","family":"Hyman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lewis","family":"Semprini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,12,19]]},"reference":[{"key":"13569_CR1","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1128\/aem.57.1.228-235.1991","volume":"57","author":"L Alvarez-Cohen","year":"1991","unstructured":"Alvarez-Cohen L, McCarty PL (1991) Effects of toxicity, aeration, and reductant supply on trichloroethylene transformation by a mixed methanotrophic culture. Appl Environ Microbiol 57:228\u2013235","journal-title":"Appl Environ Microbiol"},{"key":"13569_CR2","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1023\/A:1012075322466","volume":"12","author":"L Alvarez-Cohen","year":"2001","unstructured":"Alvarez-Cohen L, Speitel GE (2001) Kinetics of aerobic cometabolism of chlorinated solvents. Biodegradation 12:105\u2013126","journal-title":"Biodegradation"},{"key":"13569_CR3","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1023\/A:1012089908518","volume":"12","author":"DJ Arp","year":"2001","unstructured":"Arp DJ, Yeager CM, Hyman MR (2001) Molecular and cellular fundamentals of aerobic cometabolism of trichloroethylene. Biodegradation 12:81\u2013103","journal-title":"Biodegradation"},{"key":"13569_CR4","doi-asserted-by":"publisher","first-page":"1463","DOI":"10.1002\/jctb.1567","volume":"81","author":"F Aulenta","year":"2006","unstructured":"Aulenta F, Majone M, Tandoi V (2006) Enhanced anaerobic bioremediation of chlorinated solvents: environmental factors influencing microbial activity and their relevance under field conditions. J Chem Technol Biotechnol 81:1463\u20131474","journal-title":"J Chem Technol Biotechnol"},{"key":"13569_CR5","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/j.jconhyd.2006.09.015","volume":"90","author":"MF Azizian","year":"2007","unstructured":"Azizian MF, Istok JD, Semprini L (2007) Evaluation of the in-situ aerobic cometabolism of chlorinated ethenes by toluene-utilizing microorganisms using push\u2013pull tests. J Contam Hydrol 90:105\u2013124","journal-title":"J Contam Hydrol"},{"key":"13569_CR6","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.jconhyd.2010.02.004","volume":"113","author":"MF Azizian","year":"2010","unstructured":"Azizian MF, Marshall IPG, Behrens S, Spormann AM, Semprini L (2010) Comparison of lactate, formate, and propionate as hydrogen donors for the reductive dehalogenation of trichloroethene in a continuous-flow column. J Contam Hydrol 113:77\u201392","journal-title":"J Contam Hydrol"},{"key":"13569_CR7","doi-asserted-by":"publisher","first-page":"2270","DOI":"10.1128\/AEM.03556-15","volume":"82","author":"K Bennett","year":"2016","unstructured":"Bennett K, Sadler NC, Wright AT, Yeager C, Hyman MR (2016) Activity-based protein profiling of ammonia monooxygenase in Nitrosomonas europaea. Appl Environ Microbiol 82:2270\u20132279","journal-title":"Appl Environ Microbiol"},{"key":"13569_CR8","doi-asserted-by":"publisher","first-page":"10085","DOI":"10.1074\/jbc.275.14.10085","volume":"275","author":"B B\u00fchler","year":"2000","unstructured":"B\u00fchler B, Schmid A, Hauer B, Witholt B (2000) Xylene monooxygenase catalyzes the multistep oxygenation of toluene and pseudocumene to corresponding alcohols, aldehydes, and acids in Escherichia coli JM101. J Biol Chem 275:10085\u201310092","journal-title":"J Biol Chem"},{"key":"13569_CR9","doi-asserted-by":"publisher","first-page":"1279","DOI":"10.1128\/aem.56.5.1279-1285.1990","volume":"56","author":"BR Folsom","year":"1990","unstructured":"Folsom BR, Chapman PJ, Pritchard PH (1990) Phenol and trichloroethylene degradation by Pseudomonas cepacia G4: kinetics and interactions between substrates. Appl Environ Microbiol 56:1279\u20131285","journal-title":"Appl Environ Microbiol"},{"key":"13569_CR10","doi-asserted-by":"publisher","first-page":"382","DOI":"10.1016\/j.jhazmat.2014.09.041","volume":"283","author":"D Frascari","year":"2015","unstructured":"Frascari D, Zanaroli G, Danko AS (2015) In situ aerobic cometabolism of chlorinated solvents: a review. J Hazard Mater 283:382\u2013399","journal-title":"J Hazard Mater"},{"key":"13569_CR11","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1021\/es00156a012","volume":"21","author":"JM Gossett","year":"1987","unstructured":"Gossett JM (1987) Measurement of Henry\u2019s law constants for C1 and C2 chlorinated hydrocarbons. Environ Sci Technol 21:202\u2013208","journal-title":"Environ Sci Technol"},{"key":"13569_CR12","doi-asserted-by":"publisher","first-page":"1628","DOI":"10.1021\/es00006a029","volume":"29","author":"GD Hopkins","year":"1995","unstructured":"Hopkins GD, McCarty PL (1995) Field evaluation of in situ aerobic cometabolism of trichloroethylene and three dichloroethylene isomers using phenol and toluene as the primary substrates. Environ Sci Technol 29:1628\u20131637","journal-title":"Environ Sci Technol"},{"key":"13569_CR13","doi-asserted-by":"publisher","first-page":"2542","DOI":"10.1021\/es00048a035","volume":"27","author":"GD Hopkins","year":"1993","unstructured":"Hopkins GD, Munakata J, Semprini L, McCarty PL (1993) Trichloroethylene concentration effects on pilot field-scale in-situ groundwater bioremediation by phenol-oxidizing microorganisms. Environ Sci Technol 27:2542\u20132547","journal-title":"Environ Sci Technol"},{"key":"13569_CR14","doi-asserted-by":"publisher","first-page":"2277","DOI":"10.1128\/aem.59.7.2277-2285.1993","volume":"59","author":"GD Hopkins","year":"1993","unstructured":"Hopkins GD, Semprini L, McCarty PL (1993) Microcosm and in situ field studies of enhanced biotransformation of trichloroethylene by phenol-utilizing microorganisms. Appl Environ Microbiol 59:2277\u20132285","journal-title":"Appl Environ Microbiol"},{"key":"13569_CR15","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1128\/JB.183.1.405-409.2001","volume":"183","author":"RM Jones","year":"2001","unstructured":"Jones RM, Williams PA (2001) AreCBA is an operon in Acinetobacter sp. strain ADP1 and is controlled by AreR, a \u03c3(54) -dependent regulator. J Bacteriol 183:405\u2013409","journal-title":"J Bacteriol"},{"key":"13569_CR16","doi-asserted-by":"publisher","first-page":"4568","DOI":"10.1128\/JB.181.15.4568-4575.1999","volume":"181","author":"RM Jones","year":"1999","unstructured":"Jones RM, Collier LS, Neidle EL, Williams PA (1999) areABC genes determine the catabolism of eryl ester in Acinetobacter sp. strain ADP1. J Bacteriol 181:4568\u20134575","journal-title":"J Bacteriol"},{"key":"13569_CR17","doi-asserted-by":"publisher","first-page":"825","DOI":"10.1128\/aem.62.3.825-833.1996","volume":"62","author":"JG Leahy","year":"1996","unstructured":"Leahy JG, Byrne AM, Olsen RH (1996) Comparison of factors influencing trichloroethylene degradation by toluene-oxidizing bacteria. Appl Environ Microbiol 62:825\u2013833","journal-title":"Appl Environ Microbiol"},{"key":"13569_CR18","doi-asserted-by":"publisher","first-page":"1270","DOI":"10.1128\/AEM.64.4.1270-1275.1998","volume":"64","author":"JK Magnuson","year":"1998","unstructured":"Magnuson JK, Stern RV, Gossett JM, Zinder SH, Burris DR (1998) Reductive dechlorination of tetrachloroethene to ethene by a two-component enzyme pathway. Appl Environ Microbiol 64:1270\u20131275","journal-title":"Appl Environ Microbiol"},{"key":"13569_CR19","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/j.ygeno.2014.02.004","volume":"103","author":"I Maida","year":"2014","unstructured":"Maida I, Fondi M, Orlandini V, Emilian G, Papaleo MC, Perrin E, Fani R (2014) Origin, duplication and reshuffling of plasmid genes: insights from Burkholderia vietnamiensis G4 genome. Genomics 103:229\u2013238","journal-title":"Genomics"},{"key":"13569_CR20","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1016\/j.biortech.2013.05.031","volume":"142","author":"L Marang","year":"2013","unstructured":"Marang L, Jiang Y, van Loosdrecht KR (2013) Butyrate as the preferred substrate for polyhydroxybutyrate production. Bioresour Technol 142:232\u2013239","journal-title":"Bioresour Technol"},{"key":"13569_CR21","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1021\/es970322b","volume":"32","author":"PL McCarty","year":"1998","unstructured":"McCarty PL, Goltz MN, Hopkins GD, Dolan ME, Allan JP, Kawakami BT, Carrothers TJ (1998) Full-scale evaluation of in situ cometabolic degradation of trichloroethylene in groundwater through toluene injection. Environ Sci Technol 32:88\u2013100","journal-title":"Environ Sci Technol"},{"key":"13569_CR22","doi-asserted-by":"publisher","first-page":"384","DOI":"10.1016\/j.ces.2017.08.011","volume":"173","author":"AM Nassimi","year":"2017","unstructured":"Nassimi AM, Jafari M, Farrokhpour H, Keshavarz MH (2017) Constants of explosive limits. Chem Eng Sci 173:384\u2013389","journal-title":"Chem Eng Sci"},{"key":"13569_CR23","doi-asserted-by":"publisher","first-page":"949","DOI":"10.1128\/aem.53.5.949-954.1987","volume":"53","author":"MJ Nelson","year":"1987","unstructured":"Nelson MJ, Montgomery SO, Mahaffey WR, Pritchard PH (1987) Biodegradation of trichloroethylene and involvement of an aromatic biodegradative pathway. Appl Environ Microbiol 53:949\u2013954","journal-title":"Appl Environ Microbiol"},{"key":"13569_CR24","doi-asserted-by":"publisher","first-page":"14066","DOI":"10.1021\/bi00043a012","volume":"34","author":"LM Newman","year":"1995","unstructured":"Newman LM, Wackett LP (1995) Purification and characterization of toluene-2-monooxygenase from Burkholderia cepacia G4. Biochemistry 34:14066\u201314076","journal-title":"Biochemistry"},{"key":"13569_CR25","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1128\/jb.179.1.90-96.1997","volume":"179","author":"LM Newman","year":"1997","unstructured":"Newman LM, Wackett LP (1997) Trichloroethylene oxidation by purified toluene-2-monooxygenase: products, kinetics, and turnover-dependent inactivation. J Bacteriol 179:90\u201396","journal-title":"J Bacteriol"},{"key":"13569_CR26","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnad002","volume":"370","author":"MA Oyarzun","year":"2023","unstructured":"Oyarzun MA, Hyman MR (2023) Diyne inactivators and activity-based fluorescent labeling of phenol hydroxylase in Pseudomonas\n sp. CF600. FEMS Microbiol Lett 370:fnad002","journal-title":"FEMS Microbiol Lett"},{"key":"13569_CR27","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1007\/s10295-011-1040-6","volume":"39","author":"W Pan","year":"2012","unstructured":"Pan W, Perrotta JA, Stipanovic AJ, Nomura CT, Nakas JP (2012) Production of polyhydroxyalkanoates by Burkholderia cepacia ATCC 17759 using a detoxified sugar maple hemicellulosic hydrolysate. J Ind Microbiol Biotechnol 39:459\u2013469","journal-title":"J Ind Microbiol Biotechnol"},{"key":"13569_CR28","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0065-2164(08)00401-2","volume":"64","author":"RE Parales","year":"2008","unstructured":"Parales RE, Parales JV, Pelletier DA, Jl D (2008) Diversity of microbial toluene degradation pathways. Adv Appl Microbiol 64:1\u201373","journal-title":"Adv Appl Microbiol"},{"key":"13569_CR29","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1128\/aem.55.3.584-589.1989","volume":"55","author":"BA Ramsay","year":"1989","unstructured":"Ramsay BA, Ramsay JA, Cooper DG (1989) Production of poly-\u03b2-hydroxyalkanoic acid by Pseudomonas cepacia. Appl Environ Microbiol 55:584\u2013589","journal-title":"Appl Environ Microbiol"},{"key":"13569_CR30","doi-asserted-by":"publisher","first-page":"771","DOI":"10.1039\/C9EM00607A","volume":"22","author":"MT Rasmussen","year":"2020","unstructured":"Rasmussen MT, Saito A, Hyman MR, Semprini L (2020) Co-encapsulation of slow-release compounds and Rhodococcus rhodochrous ATCC 21198 in gellan gum beads to promote the long-term aerobic cometabolic transformation of 1,1,1-trichloroethane, cis-1,2-dichloroethene and 1,4-dioxane. Environ Sci Process Impacts 22:771\u2013791","journal-title":"Environ Sci Process Impacts"},{"key":"13569_CR31","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1016\/S0958-1669(97)80007-9","volume":"8","author":"L Semprini","year":"1997","unstructured":"Semprini L (1997) Strategies for the aerobic co-metabolism of chlorinated solvents. Curr Opin Biotechnol 8:296\u2013308","journal-title":"Curr Opin Biotechnol"},{"key":"13569_CR32","doi-asserted-by":"publisher","first-page":"1051","DOI":"10.1029\/94WR02380","volume":"31","author":"L Semprini","year":"1995","unstructured":"Semprini L, Kitanidis PK, Kampbell DH, Wilson JT (1995) Anaerobic transformation of chlorinated aliphatic hydrocarbons in a sand aquifer based on spatial chemical distributions. Water Res Research 31:1051\u20131062","journal-title":"Water Res Research"},{"key":"13569_CR33","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1016\/S0922-338X(97)81915-0","volume":"84","author":"H Shi","year":"1997","unstructured":"Shi H, Shiraishi M, Shimizu K (1997) Metabolic flux analysis for biosynthesis of poly(\u03b2-hydroxybutyric acid) in Alcaligenes eutrophus from various carbon sources. J Ferment Bioeng 84:579\u2013587","journal-title":"J Ferment Bioeng"},{"key":"13569_CR34","doi-asserted-by":"publisher","first-page":"1352","DOI":"10.1128\/aem.61.4.1352-1356.1995","volume":"61","author":"MS Shields","year":"1995","unstructured":"Shields MS, Reagin MJ, Gerger RR, Campbell R, Somerville C (1995) TOM, a new aromatic degradative plasmid from Burkholderia (Pseudomonas) cepacia G4. Appl Environ Microbiol 61:1352\u20131356","journal-title":"Appl Environ Microbiol"},{"key":"13569_CR35","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1002\/1097-0290(20001220)70:6<693::AID-BIT12>3.0.CO;2-W","volume":"70","author":"H Shim","year":"2000","unstructured":"Shim H, Wood TK (2000) Aerobic degradation of mixtures of chlorinated aliphatics by cloned toluene-o-xylene monooxygenase and toluene o-monooxygenase in resting cells. Biotechnol Bioeng 70:693\u2013698","journal-title":"Biotechnol Bioeng"},{"key":"13569_CR36","doi-asserted-by":"publisher","first-page":"3814","DOI":"10.1128\/AEM.70.7.3814-3820.2004","volume":"70","author":"Y Tao","year":"2004","unstructured":"Tao Y, Fishman A, Bentley WE, Wood TK (2004) Oxidation of benzene to phenol, catechol, and 1,2,3-trihydroxybenzene by toluene-4-monooxygenase of Pseudomonas mendocina KR1 and toluene-3-monooxygenase of Ralstonia pickettii PK01. Appl Environ Microbiol 70:3814\u20133820","journal-title":"Appl Environ Microbiol"},{"key":"13569_CR37","unstructured":"Tejasen S (2003) Aerobic biotransformation of chlorinated aliphatic hydrocarbons by a benzyl alcohol grown mixed culture: cometabolism, mechanisms, kinetics, and modeling. PhD. Thesis. Oregon State University"},{"key":"13569_CR38","doi-asserted-by":"publisher","first-page":"632","DOI":"10.1128\/AEM.65.2.632-639.1999","volume":"65","author":"CM Yeager","year":"1999","unstructured":"Yeager CM, Bottomley PJ, Arp DJ, Hyman MR (1999) Inactivation of toluene 2-monooxygenase in Burkholderia cepacia G4 by alkynes. Appl Environ Microbiol 65:632\u2013639","journal-title":"Appl Environ Microbiol"},{"key":"13569_CR39","doi-asserted-by":"publisher","first-page":"5384","DOI":"10.1128\/AEM.67.12.5384-5391.2001","volume":"67","author":"CM Yeager","year":"2001","unstructured":"Yeager CM, Bottomley PJ, Arp DJ (2001) Requirement of DNA repair mechanisms for survival of Burkholderia cepacia G4 upon degradation of trichloroethylene. Appl Environ Microbiol 67:5384\u20135391","journal-title":"Appl Environ Microbiol"},{"key":"13569_CR40","doi-asserted-by":"publisher","first-page":"2107","DOI":"10.1128\/AEM.67.5.2107-2115.2001","volume":"67","author":"CM Yeager","year":"2001","unstructured":"Yeager CM, Bottomley PJ, Arp DJ (2001) Cytotoxicity associated with trichloroethylene oxidation in Burkholderia cepacia G4. Appl Environ Microbiol 67:2107\u20132115","journal-title":"Appl Environ Microbiol"},{"key":"13569_CR41","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1002\/bit.20260","volume":"88","author":"S Yu","year":"2004","unstructured":"Yu S, Semprini L (2004) Kinetics and modeling of reductive dechlorination at high PCE and TCE concentrations. Biotech Bioeng 88:451\u2013464","journal-title":"Biotech Bioeng"},{"key":"13569_CR42","doi-asserted-by":"crossref","unstructured":"Zogorski JS, Carter JM, Ivahnenko T, Lapham WW, Moran MJ, Rowe BL, Squillace PJ, Toccalino PL (2006) The quality of our nations waters: volatile organic compounds in the nation\u2019s ground water and drinking-water supply wells. U.S. Geological Survey Circular 1292, 101p","DOI":"10.3133\/cir1292"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00253-025-13569-0","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-025-13569-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-025-13569-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T12:31:04Z","timestamp":1770294664000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00253-025-13569-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,19]]},"references-count":42,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["13569"],"URL":"https:\/\/doi.org\/10.1007\/s00253-025-13569-0","relation":{},"ISSN":["1432-0614"],"issn-type":[{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,19]]},"assertion":[{"value":"9 April 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 July 2025","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 July 2025","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 December 2025","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This work did not involve the studies of humans or animal subjects.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"The authors agreed on the publication.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.\nThe authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}],"article-number":"271"}}