{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T02:06:50Z","timestamp":1770343610109,"version":"3.49.0"},"reference-count":71,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T00:00:00Z","timestamp":1765411200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T00:00:00Z","timestamp":1765497600000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      The growing demand for sustainable agricultural pest control solutions has increased the use of microbial bioinput. This study characterized an industrial bioinput produced by\n                      <jats:italic>Saccharopolyspora spinosa<\/jats:italic>\n                      , focusing on identifying metabolites and quantifying spinosyns A and D by HPLC-MS. The concentrations of these compounds were 29.4\u2009\u00b1\u20093.6\u00a0mg L\n                      <jats:sup>\u22121<\/jats:sup>\n                      and 13.3\u2009\u00b1\u20091.5\u00a0mg L\n                      <jats:sup>\u22121<\/jats:sup>\n                      , respectively, for a total of 42.7\u00a0mg L\n                      <jats:sup>\u22121<\/jats:sup>\n                      . The limit of quantification (LOQ) and limit of detection (LOD) values obtained were 13.7\u00a0ng L\n                      <jats:sup>\u22121<\/jats:sup>\n                      and 4.16\u00a0ng L\n                      <jats:sup>\u22121<\/jats:sup>\n                      for spinosyn A and 7.63\u00a0ng L\n                      <jats:sup>\u22121<\/jats:sup>\n                      and 2.31\u00a0ng L\n                      <jats:sup>\u22121<\/jats:sup>\n                      for spinosyn D, respectively. The bioinput contained essential amino acids (leucine, phenylalanine, and tryptophan), plant cell death inducers (sphinganine), diketopiperazines with insecticidal potential (Cyclo(Met-Val)), larvicides (\n                      <jats:italic>N<\/jats:italic>\n                      -stearoyl tryptophan), antimicrobials (Penicitrinol D), and phospholipids associated with cell defense and stress responses (phosphatidylethanolamine, phosphatidylserine, ceramides, and mycolactones).\n                      <jats:italic>In vitro<\/jats:italic>\n                      trials demonstrated mortalities of 82.5% for\n                      <jats:italic>Spodoptera frugiperda<\/jats:italic>\n                      , 100.0% for\n                      <jats:italic>Dalbulus maidis<\/jats:italic>\n                      , and 64.1% for\n                      <jats:italic>Ceratitis capitata<\/jats:italic>\n                      . These results are statistically equivalent to those obtained using commercial products, which cost up to five times more. This bioinput\u2019s chemical diversity suggests multifunctional action, probable synergism, and a lower risk of resistance, which reinforces its potential in biological agricultural management.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      \u2022\u00a0\n                      <jats:italic>Identification of a diverse set of bioactive compounds, including spinosyns A and D.<\/jats:italic>\n                    <\/jats:p>\n                    <jats:p>\n                      \u2022\u00a0\n                      <jats:italic>Bioinput achieves statistical efficacy equal to commercial products in in vitro tests.<\/jats:italic>\n                    <\/jats:p>\n                    <jats:p>\n                      \u2022\u00a0\n                      <jats:italic>A sustainable, cost-effective alternative for large-scale production and application.<\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00253-025-13655-3","type":"journal-article","created":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T21:35:07Z","timestamp":1765488907000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chemical composition and bioinsecticidal activity of bioinputs produced by Saccharopolyspora spinosa"],"prefix":"10.1007","volume":"109","author":[{"given":"Lu\u00eds Felipe Rodrigues","family":"Costa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda Evelyn","family":"Miranda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rliton Pereira","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge Luiz Soares","family":"dos Anjos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda Menezes","family":"Maia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiara","family":"da Costa Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario Machado","family":"Martins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseilton Faria","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isac Pereira Soares","family":"Martins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clarice Diniz","family":"Alvarenga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marlon Cristian Toledo","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nelson","family":"de Abreu Delvaux J\u00fanior","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciano Pereira","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,12,11]]},"reference":[{"key":"13655_CR1","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1093\/jee\/18.2.265a","volume":"18","author":"WS Abbott","year":"1925","unstructured":"Abbott WS (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol 18:265\u2013267","journal-title":"J Econ Entomol"},{"key":"13655_CR2","doi-asserted-by":"crossref","first-page":"71","DOI":"10.21608\/eajbsf.2015.17241","volume":"7","author":"MM Azab","year":"2015","unstructured":"Azab MM (2015) Combined activity of spinosyns A, D, J and L on two stored product insects. Egypt Acad J Biol Sci F Toxicol Pest Control 7:71\u201380","journal-title":"Egypt Acad J Biol Sci F Toxicol Pest Control"},{"key":"13655_CR3","doi-asserted-by":"crossref","first-page":"40","DOI":"10.4081\/jear.2016.5653","volume":"48","author":"L Bacci","year":"2016","unstructured":"Bacci L, Lupi D, Savoldelli S, Rossaro B (2016) A review of spinosyns, a derivative of biological acting substances as a class of insecticides with a broad range of action against many insect pests. J Entomol Acarol Res 48:40\u201352","journal-title":"J Entomol Acarol Res"},{"key":"13655_CR4","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1590\/1983-40632018v4852480","volume":"48","author":"MM Baldin","year":"2018","unstructured":"Baldin MM (2018) Concentration and lethal time of toxic baits based on spinosyns on Ceratitis capitata and Diachasmimorpha longicaudata. Pesqui Agropecu Trop 48:323\u2013330","journal-title":"Pesqui Agropecu Trop"},{"key":"13655_CR5","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1007\/s13744-018-0653-0","volume":"48","author":"CA Baronio","year":"2019","unstructured":"Baronio CA, Bernardi D, Nunes MZ, Pasinato J, Garcia FRM, Botton M (2019) Bioassay method for toxicity studies of toxic bait formulations to Ceratitis capitata (Diptera: Tephritidae). Neotrop Entomol 48:356\u2013363","journal-title":"Neotrop Entomol"},{"key":"13655_CR6","doi-asserted-by":"crossref","first-page":"26640","DOI":"10.1039\/D3RA04086K","volume":"13","author":"D Basumatary","year":"2023","unstructured":"Basumatary D, Bailung H, Jorvekar SB, Borkar RM, Sankaranarayanan K (2023) Investigating the impact of inbuilt cold atmospheric pressure plasma on molecular assemblies of tryptophan enantiomers: in vitro fabrication of self-assembled supramolecular structures. RSC Adv 13:26640\u201326649","journal-title":"RSC Adv"},{"key":"13655_CR7","first-page":"326","volume":"19","author":"MA Block","year":"2007","unstructured":"Block MA, Dorne AJ, Joyard J, Douce R (2007) Probing Arabidopsis chloroplast diacylglycerol pools by selectively targeting diacylglycerol kinase to the envelope membrane. Plant Cell 19:326\u2013340","journal-title":"Plant Cell"},{"key":"13655_CR8","doi-asserted-by":"crossref","DOI":"10.14295\/BA.v10.134","volume":"10","author":"R Borges","year":"2015","unstructured":"Borges R, Machota R Jr, Boff MIC, Botton M (2015) Effect of toxic baits on Anastrepha fraterculus (Wiedemann) (Diptera: Tephritidae). BioAssay 10:134","journal-title":"BioAssay"},{"key":"13655_CR9","volume":"35","author":"AFV Braga","year":"2024","unstructured":"Braga AFV, Ros\u00e1rio MSD, Gomes JBN, Monteiro CDA, Farias FA, Rodrigues Filho E, Cantanhede Filho AJ (2024) Antimicrobial potential of soil\/sediment mangrove associated fungi: a review. J Braz Chem Soc 35:e-20240032","journal-title":"J Braz Chem Soc"},{"key":"13655_CR10","doi-asserted-by":"crossref","first-page":"5816","DOI":"10.1021\/acs.jafc.0c01172","volume":"68","author":"A Bravo","year":"2020","unstructured":"Bravo A, Sober\u00f3n M, G\u00f3mez I (2020) Cry1Ac protoxin and its activated toxin from Bacillus thuringiensis act differentially during the pathogenic process. J Agric Food Chem 68:5816\u20135824","journal-title":"J Agric Food Chem"},{"key":"13655_CR11","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1248\/cpb.59.515","volume":"59","author":"L Chen","year":"2011","unstructured":"Chen L, Liu W, Hu X, Huang K, Wu JL, Zhang QQ (2011) Citrinin derivatives from the marine-derived fungus Penicillium citrinum. Chem Pharm Bull 59:515\u2013520","journal-title":"Chem Pharm Bull"},{"key":"13655_CR12","doi-asserted-by":"crossref","first-page":"10141","DOI":"10.1007\/s00253-015-6871-z","volume":"99","author":"J Chen","year":"2015","unstructured":"Chen J, Xia H, Dang F, Xu Q, Li W, Qin Z (2015) Characterization of the chromosomal integration of Saccharopolyspora plasmid pCM32 and its application to improve production of spinosyn in Saccharopolyspora spinosa. Appl Microbiol Biotechnol 99:10141\u201310149","journal-title":"Appl Microbiol Biotechnol"},{"key":"13655_CR13","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/BF02825358","volume":"48","author":"I Chet","year":"1994","unstructured":"Chet I, Inbar J (1994) Biological control of fungal pathogens. Appl Biochem Biotechnol 48:37\u201343","journal-title":"Appl Biochem Biotechnol"},{"key":"13655_CR14","first-page":"1255","volume":"69","author":"V Choub","year":"2021","unstructured":"Choub V, Maung CEH, Won S-J, Park S-H, Ahn Y-S (2021) Antifungal activity of cyclic tetrapeptide from Bacillus velezensis CE 100 against plant pathogen Colletotrichum gloeosporioides. J Agric Food Chem 69:1255\u20131262","journal-title":"J Agric Food Chem"},{"key":"13655_CR15","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1002\/ps.424","volume":"58","author":"CB Cleveland","year":"2002","unstructured":"Cleveland CB, Mayes MA, Cryer SA (2002) An ecological risk assessment for Spinosad use on cotton. Pest Manag Sci 58:70\u201384","journal-title":"Pest Manag Sci"},{"key":"13655_CR16","unstructured":"CropLife Brasil (2025) Adoption of bioinputs grew 13% in the 2024\/2025 crop year. CropLife Brasil. https:\/\/croplifebrasil.org\/adocao-de-bioinsumos-cresceu-13-na-safra-2024-2025\/. Accessed 7 Jun 2025"},{"key":"13655_CR17","unstructured":"D\u00edaz SA, Leal GN, Silva NF, Ven\u00e2ncio MGS, Figueiredo KG, Oliveira JAC, Lopes NJ (2024) Spodoptera frugiperda: a significant threat to Brazilian agriculture. Revista Cultivar. https:\/\/revistacultivar.com\/articles\/Spodoptera-frugiperda-is-a-significant-threat-to-Brazilian-agriculture. Accessed 27 Jun 2025"},{"issue":"4","key":"13655_CR18","volume":"23","author":"EFSA","year":"2025","unstructured":"EFSA (2025) Conclusion on the peer review of the pesticide risk assessment of the active substance Spinosad. EFSA J 23(4):e9193","journal-title":"EFSA J"},{"issue":"165","key":"13655_CR19","first-page":"58632","volume":"88","author":"EPA","year":"2023","unstructured":"EPA (2023) Spinosad. Pesticide tolerances. Fed Regis 88(165):58632\u201358640","journal-title":"Pesticide tolerances. Fed Regis"},{"key":"13655_CR20","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1007\/s13744-023-01033-5","volume":"52","author":"M Faria","year":"2023","unstructured":"Faria M, Santos MA, Santos W, Rodrigues B, Andrade Z (2023) On-farm production of microbial entomopathogens for use in agriculture: Brazil as a case study. Neotrop Entomol 52:122\u2013133","journal-title":"Neotrop Entomol"},{"key":"13655_CR21","unstructured":"Ferreira DF (2000) Statistical analyses using SISVAR (System for Analysis of Variance) for Windows 4.0. In: Proceedings of the 45th nnual Meeting of the Brazilian Region of the International Biometric Society. UFSCar, S\u00e3o Carlos, pp 255\u2013258"},{"key":"13655_CR22","doi-asserted-by":"crossref","first-page":"2952","DOI":"10.4236\/am.2014.519280","volume":"5","author":"EB Ferreira","year":"2014","unstructured":"Ferreira EB, Cavalcanti PP, Nogueira DA (2014) ExpDes: an R package for ANOVA and experimental designs. Appl Math 5:2952\u20132958","journal-title":"Appl Math"},{"key":"13655_CR23","doi-asserted-by":"crossref","first-page":"1885","DOI":"10.1603\/EC10365","volume":"104","author":"S Flores","year":"2011","unstructured":"Flores S, Gomez LE, Montoya P (2011) Residual control and lethal concentrations of GF-120 (Spinosad) for Anastrepha spp. (Diptera: Tephritidae). J Econ Entomol 104:1885\u20131891","journal-title":"J Econ Entomol"},{"key":"13655_CR24","unstructured":"Gon\u00e7alves F (2025) The evolution of bioinputs: quality makes the difference. Globo Rural. https:\/\/globorural.globo.com\/opiniao\/noticia\/2025\/05\/a-evolucao-dos-bioinsumos-qualidade-faz-a-diferenca.ghtml. Accessed 5 Jun 2025"},{"key":"13655_CR25","unstructured":"Gon\u00e7alves EP, Cruz I, Figueiredo MLC, Ciociola AI (1997) Effect of \u2018Spinosad\u2019 on Spodoptera frugiperda Smith larvae and its natural enemies, the predator Doru luteipes Scudder and the parasitoid Campoletis flavicincta Ashmead. In: 16th Brazilian Congress of Entomology, Salvador. Abstracts. Salvador: SEB\/EMBRAPA-CNPMF, p 177"},{"key":"13655_CR26","doi-asserted-by":"crossref","first-page":"2760","DOI":"10.1080\/14786419.2016.1154055","volume":"30","author":"S Gopalakrishnan","year":"2016","unstructured":"Gopalakrishnan S, Rajendran V, Arumugam S, Sharma HC, Vadlamudi S, Bhimineni RK, Simic N (2016) Insecticidal activity of a novel fatty acid amide derivative from Streptomyces species against Helicoverpa armigera. Nat Prod Res 30:2760\u20132769","journal-title":"Nat Prod Res"},{"key":"13655_CR27","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.jchromb.2019.06.019","volume":"1124","author":"A Gotthardt","year":"2019","unstructured":"Gotthardt A, Bury D, Kling H-W, Weiss T, Belov V, Qi Y, Koch HM (2019) Determination of human urinary metabolites of the plasticizer di(2-ethylhexyl) adipate (DEHA) by online-SPE-HPLC-MS\/MS. J Chromatogr B 1124:239\u2013246","journal-title":"J Chromatogr B"},{"key":"13655_CR28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13071-021-05068-8","volume":"14","author":"AM Hafez","year":"2021","unstructured":"Hafez AM, Abbas N (2021) Insecticide resistance to insect growth regulators, avermectins, spinosyns, and diamides in Culex quinquefasciatus in Saudi Arabia. Parasit Vectors 14:1\u20139","journal-title":"Parasit Vectors"},{"key":"13655_CR29","doi-asserted-by":"crossref","first-page":"5533","DOI":"10.1007\/s12010-023-04765-7","volume":"196","author":"HE Hezakiel","year":"2024","unstructured":"Hezakiel HE, Thampi M, Rebello S, Sheikhmoideen JM (2024) Biopesticides: a green approach towards agricultural pests. Appl Biochem Biotechnol 196:5533\u20135562","journal-title":"Appl Biochem Biotechnol"},{"key":"13655_CR30","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/s00253-008-1784-8","volume":"82","author":"K Huang","year":"2009","unstructured":"Huang K, Xia L, Zhang Y, Ding X, Zahn JA (2009) Recent advances in the biochemistry of spinosyns. Appl Microbiol Biotechnol 82:13\u201323","journal-title":"Appl Microbiol Biotechnol"},{"key":"13655_CR31","doi-asserted-by":"crossref","first-page":"727","DOI":"10.14348\/molcells.2014.0168","volume":"37","author":"AK Jha","year":"2014","unstructured":"Jha AK, Jang JP, Shin YK, Lee BR, Cho JH, Choi YJ (2014) Metabolic engineering of rational screened Saccharopolyspora spinosa for the enhancement of spinosyns A and D production. Mol Cells 37:727\u2013733","journal-title":"Mol Cells"},{"key":"13655_CR32","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1007\/s12010-008-8500-0","volume":"159","author":"ZH Jin","year":"2009","unstructured":"Jin ZH, Wang F, Li H, Chen Z (2009) Enhanced production of Spinosad in Saccharopolyspora spinosa by genome shuffling. Appl Biochem Biotechnol 159:655\u2013663","journal-title":"Appl Biochem Biotechnol"},{"key":"13655_CR33","unstructured":"JMPR\/FAO\/OMS (2001). Spinosad. In: Pesticide Residues in Food - 2001, Toxicological Evaluations. Joint Meeting of the FAO Panel of Experts on Pesticide Residues in Food and the Environment and the WHO Core Assessment Group (JMPR). Available at\u00a0https:\/\/www.inchem.org\/documents\/jmpr\/jmpmono\/2001pr12.htm"},{"key":"13655_CR34","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1201\/9781003369868-9","volume-title":"Applied Mycology for Agriculture and Foods","author":"D Kumar","year":"2023","unstructured":"Kumar D, Singh A, Sharma P, Gupta R (2023) Fungal biofertilizers and biopesticides and their roles in sustainable agriculture. In: Gupta VK (ed) Applied Mycology for Agriculture and Foods. Apple Academic Press, Palm Bay, FL, pp 165\u2013216"},{"key":"13655_CR35","doi-asserted-by":"crossref","first-page":"5632","DOI":"10.1128\/JB.183.19.5632-5638.2001","volume":"183","author":"K Madduri","year":"2001","unstructured":"Madduri K, Waldron C, Merlo DJ (2001a) Rhamnose biosynthesis pathway supplies precursors for primary and secondary metabolism in Saccharopolyspora spinosa. J Bacteriol 183:5632\u20135638","journal-title":"J Bacteriol"},{"key":"13655_CR36","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1038\/sj.jim.7000180","volume":"27","author":"K Madduri","year":"2001","unstructured":"Madduri K, Zirkle RE, Jaskulka BT (2001b) Genes for the biosynthesis of spinosyns: applications for yield improvement in Saccharopolyspora spinosa. J Ind Microbiol Biotechnol 27:399\u2013402","journal-title":"J Ind Microbiol Biotechnol"},{"key":"13655_CR37","first-page":"1586","volume":"2","author":"K Marcinkevicius","year":"2017","unstructured":"Marcinkevicius K, Salvatore SA, Bardon ADV, Cartagena E, Arena ME, Vera NR (2017) Insecticidal activities of diketopiperazines of Nomuraea rileyi entomopathogenic fungus. Int J Environ Agric Biotechnol 2:1586\u20131596","journal-title":"Int J Environ Agric Biotechnol"},{"key":"13655_CR38","first-page":"487","volume":"190","author":"JE Markham","year":"2011","unstructured":"Markham JE, Lozano-Rosas MG, Dietrich CR, Ramos-Vega M, Cahoon EB, Gavilanes-Ru\u00edz M (2011) MPK6, sphinganine and the LCB2a gene from serine palmitoyltransferase are required in the signaling pathway that mediates cell death induced by long chain bases in Arabidopsis. New Phytol 190:487\u2013499","journal-title":"New Phytol"},{"key":"13655_CR39","first-page":"223","volume":"40","author":"FP Mertz","year":"1990","unstructured":"Mertz FP, Yao RC (1990) Saccharopolyspora spinosa sp. nov. isolated from soil. Int J Syst Bacteriol 40:223\u2013228","journal-title":"Int J Syst Bacteriol"},{"key":"13655_CR40","doi-asserted-by":"publisher","DOI":"10.1007\/s13744-019-00728-y","author":"MZ Nunes","year":"2019","unstructured":"Nunes MZ, Bernardi D, Baronio CA, Pasinato JA (2019) A laboratory bioassay method to assess the use of toxic bait on Anastrepha fraterculus (Weidemann 1830). Neotrop Entomol. https:\/\/doi.org\/10.1007\/s13744-019-00728-y","journal-title":"Neotrop Entomol"},{"key":"13655_CR41","first-page":"3947","volume":"66","author":"Y Okazaki","year":"2018","unstructured":"Okazaki Y, Saito K (2018) Roles of lipid metabolism in plant defense. J Agric Food Chem 66:3947\u20133952","journal-title":"J Agric Food Chem"},{"key":"13655_CR42","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.tim.2007.12.009","volume":"16","author":"M Ongena","year":"2008","unstructured":"Ongena M, Jacques P (2008) Bacillus lipopeptides: versatile weapons for plant disease biocontrol. Trends Microbiol 16:115\u2013125","journal-title":"Trends Microbiol"},{"key":"13655_CR43","unstructured":"P\u00fcntener W, Zahner O (1981) Manual for field trials in plant protection, 2nd edn, rev. and enl. Ciba-Geigy, Basle"},{"key":"13655_CR44","volume":"35","author":"MM Rahman","year":"2021","unstructured":"Rahman MM, El-Aty AMA, Ara J, Park J-H, Kim M-R, Eun J-B, Shin H-C, Shim J-H (2021) Quantification of spinosyn A and spinosyn D in animal-derived products using multiwalled carbon nanotubes coupled with LC\u2013MS\/MS for analysis. Biomed Chromatogr 35:e5007","journal-title":"Biomed Chromatogr"},{"key":"13655_CR45","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.3390\/su16031076","volume":"16","author":"TM Rocha","year":"2024","unstructured":"Rocha TM, Dias AHS, Lins H, Goulart C, Ribeiro G, Magalh\u00e3es M, Oliveira A (2024) Agricultural bioinputs obtained by solid-state fermentation: from production in biorefineries to sustainable agriculture. Sustainability 16:1076","journal-title":"Sustainability"},{"key":"13655_CR46","unstructured":"Rodrigues PS, dos Santos TAT, DaMatta RA, Seabra SH (2018) Evaluation of the exposure of phosphatidylserine (PS) by Plasmodium chabaudi. In: Proceedings of the 3rd Fluminense Congress of Post-Graduation. Instituto Federal de Educa\u00e7\u00e3o, Ci\u00eancia e Tecnologia (IFF), UENF, Campos dos Goytacazes. https:\/\/editoraessentia.iff.edu.br\/index.php\/CONPG\/article\/view\/13285\/10643. Accessed 27 Jun 2025"},{"key":"13655_CR47","doi-asserted-by":"crossref","first-page":"1688","DOI":"10.1093\/jee\/toz066","volume":"112","author":"GG Rolim","year":"2019","unstructured":"Rolim GG, Guedes RNC, Siqueira HAA (2019) Susceptibility of cotton boll weevil (Coleoptera: Curculionidae) to spinosyns. J Econ Entomol 112:1688\u20131694","journal-title":"J Econ Entomol"},{"key":"13655_CR48","doi-asserted-by":"crossref","first-page":"4289","DOI":"10.1021\/acsomega.0c05399","volume":"6","author":"T Sarkar","year":"2021","unstructured":"Sarkar T, Nandi S, Bhunia D, Ghosh S (2021) Menthyloxy carbonyl valine as a plant defense inducer: a new molecule for crop protection. ACS Omega 6:4289\u20134297","journal-title":"ACS Omega"},{"key":"13655_CR49","first-page":"487","volume":"190","author":"M Saucedo-Garc\u00eda","year":"2011","unstructured":"Saucedo-Garc\u00eda M, Guevara-Garc\u00eda A, Gonz\u00e1lez-Sol\u00eds A, Cruz-Garc\u00eda F, V\u00e1zquez-Santana S, Markham JE, Lozano-Rosas MG, Dietrich CR, Ramos-Vega M, Cahoon EB, Gavilanes-Ru\u00edz M (2011) MPK6, sphinganine and the LCB2a gene from serine palmitoyltransferase are required in the signaling pathway that mediates cell death induced by long chain bases in Arabidopsis. New Phytol 190:487\u2013499","journal-title":"New Phytol"},{"issue":"02","key":"13655_CR50","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1590\/s0100-204x2018000200002","volume":"53","author":"IX Sch\u00fctze","year":"2018","unstructured":"Sch\u00fctze IX, Baronio CA, Baldin MM, Loek AE, Botton M (2018) Toxicity and residual effects of toxic baits with spinosyns on the South American fruit fly. Pesqui Agropecu Bras 53(02):144\u2013151","journal-title":"Pesqui Agropecu Bras"},{"key":"13655_CR51","doi-asserted-by":"crossref","first-page":"4994","DOI":"10.3390\/ijms15034994","volume":"15","author":"R Sun","year":"2014","unstructured":"Sun R, Zhang J, Yin L, Pu Y (2014) Investigation into variation of endogenous metabolites in bone marrow cells and plasma in C3H\/He mice exposed to benzene. Int J Mol Sci 15:4994\u20135010","journal-title":"Int J Mol Sci"},{"key":"13655_CR52","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1093\/jee\/62.4.967","volume":"62","author":"N Tanaka","year":"1969","unstructured":"Tanaka N, Steiner LF, Ohinata K, Okamoto R (1969) Low-cost larval rearing medium for mass production of oriental and Mediterranean fruit flies. J Econ Entomol 62:967\u2013968","journal-title":"J Econ Entomol"},{"key":"13655_CR53","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1002\/1526-4998(200008)56:8<696::AID-PS182>3.0.CO;2-5","volume":"56","author":"GD Thompson","year":"2000","unstructured":"Thompson GD, Cleveland CB, McCormick KL (2000) Spinosad-a case study: an example from a natural products discovery programme. Pest Manag Sci 56:660\u2013664","journal-title":"Pest Manag Sci"},{"key":"13655_CR54","first-page":"588","volume":"24","author":"S Timm","year":"2012","unstructured":"Timm S, Florian A, Arrivault S, Stitt M, Fernie AR, Bauwe H (2012) Glycine decarboxylase controls photosynthesis and plant growth. Plant Cell 24:588\u2013606","journal-title":"Plant Cell"},{"key":"13655_CR55","first-page":"26","volume":"6","author":"GV Tomquelski","year":"2007","unstructured":"Tomquelski GV, Martins GLM (2007) Efici\u00eancia de inseticidas sobre Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae) em milho na regi\u00e3o dos Chapad\u00f5es. Rev Bras Milho Sorgo 6:26\u201339","journal-title":"Rev Bras Milho Sorgo"},{"key":"13655_CR56","doi-asserted-by":"crossref","DOI":"10.1016\/j.cropro.2023.106354","volume":"173","author":"TN Vassilakos","year":"2023","unstructured":"Vassilakos TN (2023) Spinetoram: a potential grain protectant. Crop Prot 173:106354","journal-title":"Crop Prot"},{"key":"13655_CR57","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.jspr.2012.06.006","volume":"51","author":"TN Vassilakos","year":"2012","unstructured":"Vassilakos TN, Athanassiou CG, Kavallieratos NG, Tomanovi\u0107 \u017d (2012) Insecticidal effect of Spinetoram against six major stored grain insect species. J Stored Prod Res 51:69\u201373","journal-title":"J Stored Prod Res"},{"key":"13655_CR58","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.pestbp.2011.04.004","volume":"100","author":"J Vontas","year":"2011","unstructured":"Vontas J, Hern\u00e1ndez-Crespo P, Van Leeuwen T, Ranson H (2011) Insecticide resistance in Tephritid flies. Pestic Biochem Physiol 100:199\u2013205","journal-title":"Pestic Biochem Physiol"},{"key":"13655_CR59","first-page":"288","volume":"1787","author":"H Wada","year":"2009","unstructured":"Wada H, Murata N (2009) Lipids in photosynthesis: essential roles in photosystem II and photosystem I. Biochim Biophys Acta 1787:288\u2013296","journal-title":"Biochim Biophys Acta"},{"key":"13655_CR60","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1002\/rcm.7466","volume":"30","author":"SC Wang","year":"2016","unstructured":"Wang SC, Chen W, Liu Y, Xu M, Wang J, Lin L, Zhang J (2016) Matrix-assisted laser desorption\/ionization mass spectrometry imaging of cell cultures for the lipidomic analysis of potential lipid markers in human breast cancer invasion. Rapid Commun Mass Spectrom 30:533\u2013542","journal-title":"Rapid Commun Mass Spectrom"},{"key":"13655_CR61","first-page":"3785","volume":"68","author":"W Wang","year":"2020","unstructured":"Wang W, Lu Z, Zhang J, Liu Q, Wang L, Wang H (2020) Lipid signaling in plant defense: roles of phosphatidylethanolamine and derivatives. J Agric Food Chem 68:3785\u20133793","journal-title":"J Agric Food Chem"},{"key":"13655_CR62","first-page":"299","volume":"2","author":"Z Xu","year":"2024","unstructured":"Xu Z, Zhang B, Liu X, Wang Q, Lin Y, Wu R, Ma R, Wang J, Lu X (2024) Breeding of butenyl-spinosyns high yielding strain by 60Co-NTG compound mutation and its insecticidal activity. Chin J Biol Control 2:299","journal-title":"Chin J Biol Control"},{"key":"13655_CR63","unstructured":"Yamamoto PT, M\u00fcller C, Bedendo IP (2020) Efficacy of insecticides in the control of Dalbulus maidis (DeLong & Wolcott) (Hemiptera: Cicadellidae) and the transmission of corn spiroplasma. PhD Thesis, University of S\u00e3o Paulo (USP), Piracicaba"},{"key":"13655_CR64","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1752-0509-8-21","volume":"8","author":"X Yang","year":"2014","unstructured":"Yang X, Niu J, Guo X (2014) Genome-scale metabolic network reconstruction of Saccharopolyspora spinosa for Spinosad production improvement. BMC Syst Biol 8:1\u201313","journal-title":"BMC Syst Biol"},{"key":"13655_CR65","volume":"9","author":"G Yang","year":"2016","unstructured":"Yang G, Cao S, Yan J, Wang Q, Ma R, Lu X (2016) A new medium for improving Spinosad production by Saccharopolyspora spinosa. Jundishapur J Microbiol 9:e16765","journal-title":"Jundishapur J Microbiol"},{"key":"13655_CR66","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.phytol.2018.04.023","volume":"25","author":"S-Q Yang","year":"2018","unstructured":"Yang S-Q, Li X-M, Li X, Li H-L, Meng L-H, Wang B-G (2018) New citrinin analogues produced by coculture of the marine algal-derived endophytic fungal strains Aspergillus sydowii EN-534 and Penicillium citrinum EN-535. Phytochemistry Lett 25:191\u2013195","journal-title":"Phytochemistry Lett"},{"key":"13655_CR67","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1590\/S0103-50532011000600018","volume":"22","author":"G Yao","year":"2011","unstructured":"Yao G, Sebisubi FM, Voo LYC, Ho CC, Tan GT, Chang LC (2011) Citrinin derivatives from the soil filamentous fungus Penicillium sp. H9318. J Braz Chem Soc 22:1125\u20131129","journal-title":"J Braz Chem Soc"},{"key":"13655_CR68","first-page":"5311","volume":"145","author":"H Ye","year":"2020","unstructured":"Ye H, Zhang Y, Chen Y, Li J, Zhang H, Luo Y, Pan J (2020) ATR-FTIR spectroscopic identification of red blood cell changes induced by sepsis. Analyst 145:5311\u20135319","journal-title":"Analyst"},{"key":"13655_CR69","doi-asserted-by":"crossref","first-page":"14779","DOI":"10.1038\/s41598-021-94251-z","volume":"11","author":"Y Zhang","year":"2021","unstructured":"Zhang Y, Liu X, Yin T, Li Q, Zou Q, Huang K, Zhang X (2021) Comparative transcriptomic analysis of two Saccharopolyspora spinosa strains reveals the relationships between primary metabolism and Spinosad production. Sci Rep 11:14779","journal-title":"Sci Rep"},{"key":"13655_CR70","doi-asserted-by":"crossref","first-page":"2415","DOI":"10.1002\/ps.5782","volume":"76","author":"Y Zuo","year":"2020","unstructured":"Zuo Y, Wu J, Li M, Zhang X, Wang H (2020) Functional validation of nicotinic acetylcholine receptor (nAChR) \u03b16 as a target of spinosyns in Spodoptera exigua utilizing the CRISPR\/Cas9 system. Pest Manag Sci 76:2415\u20132422","journal-title":"Pest Manag Sci"},{"key":"13655_CR71","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1111\/1744-7917.12922","volume":"29","author":"Y-Y Zuo","year":"2022","unstructured":"Zuo Y-Y, Li M, Wang L, Zhang X, Liu H (2022) Knockin of the G275E mutation of the nicotinic acetylcholine receptor (nAChR) \u03b16 confers high levels of resistance to spinosyns in Spodoptera exigua. Insect Sci 29:478\u2013486","journal-title":"Insect Sci"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-025-13655-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00253-025-13655-3","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-025-13655-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T12:32:28Z","timestamp":1770294748000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00253-025-13655-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,11]]},"references-count":71,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["13655"],"URL":"https:\/\/doi.org\/10.1007\/s00253-025-13655-3","relation":{},"ISSN":["1432-0614"],"issn-type":[{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,11]]},"assertion":[{"value":"23 September 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 November 2025","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 November 2025","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 December 2025","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"The authors declare that they did not use AI Technologies.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declaration of the use of AI technologies"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"267"}}