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For economic and ecological sustainability, cheap and abundant substrates such as lignocellulosic and hemicellulosic residues from agricultural or forestry side streams are preferable. Cereal brans, rich in hemicellulose, represent a promising substrate. However, for direct fermentation of this material, only low product titers are reported. In this study, we characterized the utilization of arabinoxylan, the main polysaccharide component of cereal bran, by the industrial ABE producer\n                      <jats:italic>Clostridium saccharobutylicum<\/jats:italic>\n                      DSM 13864\n                      <jats:sup>T<\/jats:sup>\n                      and report inefficient degradation of the substrate. Supplementation with hemicellulolytic enzyme mixtures derived from the thermophilic organism\n                      <jats:italic>Thermoclostridium stercorarium<\/jats:italic>\n                      subsp.\n                      <jats:italic>stercorarium<\/jats:italic>\n                      DSM 8532\n                      <jats:sup>T<\/jats:sup>\n                      significantly enhanced substrate utilization. The best improvement was achieved by the addition of the arabinofuranosidase Axh43A, which reduced the residual sugar content in the fermentation broth from 48.2 to 17.8%. Analysis of the remaining oligosaccharides after growth on arabinoxylan showed that\n                      <jats:italic>C. saccharobutylicum<\/jats:italic>\n                      cannot remove\n                      <jats:italic>O<\/jats:italic>\n                      -2 and\n                      <jats:italic>O<\/jats:italic>\n                      -3 \u03b1-L-arabinofuranosyl groups from double-substituted xyloses, creating a key bottleneck in arabinoxylan degradation that is overcome by Axh43A addition. Plasmid-based expression of Axh43A in\n                      <jats:italic>C. saccharobutylicum<\/jats:italic>\n                      DSM 13864\n                      <jats:sup>T<\/jats:sup>\n                      replicated the enzymatic supplementation effects, confirming the enzyme\u2019s role in overcoming this limitation. This underscores the potential of genetic engineering to enhance the valorization of lignocellulosic biomass in biotechnological fermentation processes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>\n                      <jats:bold>Key Points<\/jats:bold>\n                    <\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>\n                            <jats:italic>C. saccharobutylicum DSM\u00a013864<\/jats:italic>\n                            <jats:sup>T<\/jats:sup>\n                            <jats:italic>insufficiently utilizes cereal arabinoxylan.<\/jats:italic>\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            <jats:italic>Inability to cleave double-arabinosylated xylose moieties limits degradation.<\/jats:italic>\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            <jats:italic>Heterologous expression of<\/jats:italic>\n                            Axh43A\n                            <jats:italic>strongly increases arabinoxylan utilization.<\/jats:italic>\n                          <\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Graphical Abstract<\/jats:title>\n                  <\/jats:sec>","DOI":"10.1007\/s00253-025-13670-4","type":"journal-article","created":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T22:05:21Z","timestamp":1766181921000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Improvement of arabinoxylan degradation in Clostridium saccharobutylicum DSM 13864T fermentations by heterologous glycoside hydrolase supplementation and expression"],"prefix":"10.1007","volume":"109","author":[{"given":"Holger","family":"Edelmann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph","family":"Rebel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melanie","family":"Baudrexl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8421-2478","authenticated-orcid":false,"given":"Wolfgang","family":"Liebl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9580-3620","authenticated-orcid":false,"given":"Armin","family":"Ehrenreich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,12,19]]},"reference":[{"key":"13670_CR1","doi-asserted-by":"publisher","first-page":"817","DOI":"10.1007\/s00449-011-0664-2","volume":"35","author":"NKN Al-Shorgani","year":"2012","unstructured":"Al-Shorgani NKN, Kalil MS, Yusoff WMW (2012) Biobutanol production from rice bran and de-oiled rice bran by Clostridium saccharoperbutylacetonicum N1\u20134. 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