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With the aim to diversify the SCCA\/MCCA profile of fermentates for biopreservation, we designed bioprocesses employing bacterial or multi-kingdom consortia to produce caproate-containing fermentates using a targeted cross-feeding strategy. We combined\n                      <jats:italic>Limosilactobacillus reuteri, Clostridium kluyveri,<\/jats:italic>\n                      and\n                      <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                      , and quantified substrates utilization and metabolites. The antimicrobial activity of SCCA\/MCCA and fermentates was analysed in vitro and\u00a0in a meat model system. In a first bioprocess, the addition of ethanol (EtOH) initiated caproate formation by the bacterial consortium with levels of 63.9\u00a0mM (7.4\u00a0g L\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) after 22\u00a0days. Next, we run two shorter bioprocesses (12\u00a0days) and more caproate (28.9\u00a0mM, 3.4\u00a0g L\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) was produced if EtOH was regularly added than without EtOH supplementation (21.7\u00a0mM, 2.5\u00a0g L\n                      <jats:sup>\u22121<\/jats:sup>\n                      ). When\n                      <jats:italic>S. cerevisiae<\/jats:italic>\n                      was included, 37.9\u2009\u00b1\u200911.4\u00a0mM (4.4\u00a0g L\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) caproate was formed without EtOH addition. Beyond the intended cross-feeding activity, consortia produced and re-metabolised mannitol and glycerol. Lactate and caproate were the major carboxylic acids in fermentates. Caproate inhibited bacteria, yeast and molds at pH 4.5 and 6.5 in vitro, while lactate and caproate acted synergistically in minced meat. Fermentates conferred antimicrobial activity against indicator microbes mostly at pH 4.5 and to a lesser extent at pH 6.5. In this study we successfully designed a consortium to produce caproate. We provide evidence that caproate-containing fermentates had strong antimicrobial activity, and our results indicate the complexity of metabolic interactions that occurred within consortium members.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      \u2022\n                      <jats:italic>Design of a self-contained bioprocess with multi-kingdom consortium.<\/jats:italic>\n                    <\/jats:p>\n                    <jats:p>\n                      \u2022\n                      <jats:italic>Cross-feeding of bacteria and yeast to produce caproate fermentates.<\/jats:italic>\n                    <\/jats:p>\n                    <jats:p>\n                      \u2022\n                      <jats:italic>Fermentates containing caproate inhibited bacteria, yeast and mold growth.<\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00253-026-13718-z","type":"journal-article","created":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T18:05:54Z","timestamp":1772215554000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Development of novel microbial synthetic consortia for the production of antimicrobial fermentates containing caproate"],"prefix":"10.1007","volume":"110","author":[{"given":"Maria Florencia","family":"Bambace","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ker Sin","family":"Ng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten Wiborg","family":"Jensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrien","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mensure Elvan","family":"Gezer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angeliki","family":"Marietou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aviaja Kristiansen","family":"Aarseth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annika","family":"Regnet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathrine Gravlund","family":"F\u00f8nss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrik Kr\u00e6mer","family":"Sundekilde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clarissa","family":"Schwab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,27]]},"reference":[{"key":"13718_CR1","doi-asserted-by":"publisher","first-page":"2796","DOI":"10.1021\/acs.est.5b04847","volume":"50","author":"LT Angenent","year":"2016","unstructured":"Angenent LT, Richter H, Buckel W, Spirito CM, Steinbusch KJ, Plugge CM, Strik DP, Grootscholten TI, Buisman CJ, Hamelers HV (2016) Chain elongation with reactor microbiomes: open-culture biotechnology to produce biochemicals. 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