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Conventional multilayer flasks rely on scale-out approaches, require extensive manual handling, and lack monitoring process control compared to bioreactors. Here, we evaluate the CellScrew\u00ae, a dynamic 2D culture system available in three sizes ranging from 851 to 10,197 cm\n                      <jats:sup>2<\/jats:sup>\n                      , in which cell culture surfaces are continuously rotated, generating controlled hydrodynamic conditions that enhance mass transfer while reducing material consumption. Hydrodynamic conditions and oxygen transfer were characterized, with k\n                      <jats:sub>L<\/jats:sub>\n                      a values up to 6.69\u2009\u00b1\u20090.10\u00a0h\n                      <jats:sup>\u22121<\/jats:sup>\n                      . In this context, we assessed cell attachment, proliferation, metabolism, and harvest performance using human embryonic kidney 293 (HEK-293), murine melanoma (B16-F10), and murine colon carcinoma (CT-26 WT) cells, which serve as established model systems for biotherapeutic manufacturing applications such as viral vectors and vaccine production. The system supported cell densities up to 7.7\u2009\u00d7\u200910E5 cells per cm\n                      <jats:sup>2<\/jats:sup>\n                      (HEK-293), 8.7\u2009\u00d7\u200910E4 cells per cm\n                      <jats:sup>2<\/jats:sup>\n                      (B16-F10), and 1.1\u2009\u00d7\u200910E5 cells per cm\n                      <jats:sup>2<\/jats:sup>\n                      (CT-26 WT) with viabilities above 92%, comparable to multilayer flasks. Glucose consumption and lactate accumulation indicated stable metabolic activity. Mechanical harvesting enabled recovery efficiencies exceeding 89% while reducing detachment reagent usage by 67%. Overall, these results suggest that the CellScrew\u00ae can support robust adherent cell expansion with potential improvements in process efficiency and reduced resource consumption, indicating that it may represent a scalable and potentially more sustainable alternative to traditional multilayer platforms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      \u2022\n                      <jats:italic>CellScrew\u00ae reaches up to 0.77\u2009\u00b1\u20090.14\u2009\u00d7\u200910E6 cells per cm<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>2<\/jats:italic>\n                      <\/jats:sup>\n                      <jats:italic>(cell-line dependent).<\/jats:italic>\n                    <\/jats:p>\n                    <jats:p>\n                      \u2022\n                      <jats:italic>Harvest efficiency with 67% less detachment reagent use.<\/jats:italic>\n                    <\/jats:p>\n                    <jats:p>\n                      \u2022\n                      <jats:italic>High oxygen transfer (kLa up to 6.69\u2009\u00b1\u20090.10 h\u22121) enables efficient culture.<\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00253-026-13880-4","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T13:36:44Z","timestamp":1779457004000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Characterization and evaluation of a novel polylactic acid-made bioreactor for large-scale adherent cell expansion"],"prefix":"10.1007","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-7559-023X","authenticated-orcid":false,"given":"Bj\u00f6rn","family":"Boshof","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alena","family":"H\u00fcppner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3172-1036","authenticated-orcid":false,"given":"Johanna","family":"Eichberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2838-069X","authenticated-orcid":false,"given":"Henning","family":"Reyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lukas","family":"K\u00e4\u00dfer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"K\u00f6lsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,22]]},"reference":[{"key":"13880_CR1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2020.575146","volume":"7","author":"CF Bellani","year":"2020","unstructured":"Bellani CF, Ajeian J, Duffy L, Miotto M, Groenewegen L, Connon CJ (2020) Scale-up technologies for the manufacture of adherent cells. 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