{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:09:22Z","timestamp":1792782562926,"version":"4.5.0"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"vor","delay-in-days":86,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["IIRPSG-2024-01-00143"],"award-info":[{"award-number":["IIRPSG-2024-01-00143"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Manipal Academy of Higher Education, Manipal","id":[{"id":"https:\/\/ror.org\/02xzytt36","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Burkholderia cepacia<\/jats:italic>\n                      , a gram-negative bacillus, is widely recognized as an emerging pathogen responsible for nosocomial infections in patients with cystic fibrosis (CF). The conventional culture-based method takes 5\u20137\u00a0days, posing a challenge for rapid bacterial identification. Here, we developed a thin-film solid phase microextraction (TF-SPME) device by coating a fiberglass sheet with hydrophilic-lipophilic balanced (HLB) particles and polydimethylsiloxane (PDMS) polymers, exploiting an automatic film applicator to maintain coating uniformity. The coated sheet was trimmed into multiple microextraction sampling tools, and the individual patches were optimized using a McReynolds standard solution containing benzene, 2-pentanone, pyridine, octane, 1-nitropropane, and 1-pentanol compounds. Headspace sampling was performed to capture the highly volatile compounds, whereas comparatively less volatile and polar compounds were extracted through the direct immersion by the microextraction patches. The TF-SPME patches were desorbed in a gas chromatography triple quadrupole mass spectrometer, and the chromatogram revealed the emission of 34 metabolites associated with\n                      <jats:italic>B. cepacia<\/jats:italic>\n                      pathogen during the growth phase. This study demonstrated the feasibility of the utilization of simple, cost-effective microextraction patches for the extraction of\n                      <jats:italic>B. cepacia<\/jats:italic>\n                      specific metabolites. Moreover, the greenness assessment score (BAGI score\u2009=\u200970 and AGREE\u2009=\u20090.68) established the sustainability of the sample preparation technique. Further validation with clinical specimen may facilitate the future clinical research for the identification of\n                      <jats:italic>B. cepacia<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      \u2022\n                      <jats:italic>Fiberglass-based polymer-coated microextraction patches were fabricated<\/jats:italic>\n                    <\/jats:p>\n                    <jats:p>\n                      \u2022\n                      <jats:italic>Fabricated patches were optimized for capturing volatile metabolites<\/jats:italic>\n                    <\/jats:p>\n                    <jats:p>\n                      \u2022\n                      <jats:italic>Burkholderia cepacia was screened through in-vitro metabolic profiling by patches<\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00253-026-13962-3","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T13:47:32Z","timestamp":1784123252000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Design of solid phase microextraction patches for the detection of volatile metabolites associated with Burkholderia cepacia"],"prefix":"10.1007","volume":"110","author":[{"given":"Sk Rahabar","family":"Ali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debsmita","family":"Mandal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shyam Sundar","family":"Nandi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2289-1038","authenticated-orcid":false,"given":"Chiranjit","family":"Ghosh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiranjay","family":"Mukhopadhyay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,15]]},"reference":[{"key":"13962_CR1","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1007\/s10295-020-02287-3","volume":"47","author":"B Ahn","year":"2020","unstructured":"Ahn B, Gibson A, Williams P, Alusta DA, Buzatu YJ, Lee JJ, LiPuma D, Hussong B, Marasa CE, Cerniglia 2020 (2020Y) A comparison of culture-based, real-time PCR, droplet digital PCR and flow cytometric methods for the detection of Burkholderia cepacia complex in nuclease-free water and antiseptics. J Ind Microbiol Biotechnol 47:475\u2013484. https:\/\/doi.org\/10.1007\/s10295-020-02287-3","journal-title":"J Ind Microbiol Biotechnol"},{"key":"13962_CR2","doi-asserted-by":"publisher","unstructured":"Anies AJ, He J, Lefever S, Boogaerts T, Covaci A, Blust R, Town RM (2025) Sorption of psychoactive compounds on passive sampling sorbents in the presence of wastewater suspended particulate matter. Environ Pollut 385:127104.\nhttps:\/\/doi.org\/10.1016\/j.envpol.2025.127104","DOI":"10.1016\/j.envpol.2025.127104"},{"key":"13962_CR3","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11061401","volume":"11","author":"S Daddy Gaoh","year":"2023","unstructured":"Daddy Gaoh S, Kweon O, Ahn 2023 (2023S) Propidium monoazide (PMAxx)-recombinase polymerase amplification Exo (RPA Exo) assay for rapid detection of Burkholderia cepacia complex in chlorhexidine gluconate (CHX) and benzalkonium chloride (BZK) solutions. Microorganisms 11:1401. https:\/\/doi.org\/10.3390\/microorganisms11061401","journal-title":"Microorganisms"},{"key":"13962_CR4","doi-asserted-by":"publisher","unstructured":"Davis TJ, Karanjia AV, Bhebhe CN, West SB, Richardson M, Bean HD (2020) Pseudomonas aeruginosa volatilome characteristics and adaptations in chronic cystic fibrosis lung infections. mSphere 5. https:\/\/doi.org\/10.1128\/mSphere.00843-20","DOI":"10.1128\/mSphere.00843-20"},{"key":"13962_CR5","doi-asserted-by":"publisher","unstructured":"Dixon B, Ahmed WM, Mohamed AA, Felton T, Fowler SJ (2022)\u00a0Metabolic phenotyping of acquired ampicillin resistance using microbial volatiles from Escherichia coli cultures J Appl Microbiol 133:2445\u20132456.\u00a0https:\/\/doi.org\/10.1111\/jam.15716","DOI":"10.1111\/jam.15716"},{"key":"13962_CR6","doi-asserted-by":"publisher","DOI":"10.1088\/1752-7155\/10\/3\/037102","volume":"10","author":"K Dryahina","year":"2016","unstructured":"Dryahina K, Sovov\u00e1 A, Nemec P, \u0160pan\u011bl P (2016K) Differentiation of pulmonary bacterial pathogens in cystic fibrosis by volatile metabolites emitted by their in vitro cultures: Pseudomonas aeruginosa, Staphylococcus aureus, Stenotrophomonas maltophilia and the Burkholderia cepacia complex. J Breath Res 10:037102. https:\/\/doi.org\/10.1088\/1752-7155\/10\/3\/037102","journal-title":"J Breath Res"},{"key":"13962_CR7","doi-asserted-by":"publisher","first-page":"1394","DOI":"10.1021\/ac102868e","volume":"83","author":"S Endo","year":"2011","unstructured":"Endo S, Droge STJ, Goss K-U (2011S) Polyparameter linear free energy models for polyacrylate fiber\u2212water partition coefficients to evaluate the efficiency of solid-phase microextraction. Anal Chem 83:1394\u20131400. https:\/\/doi.org\/10.1021\/ac102868e","journal-title":"Anal Chem"},{"key":"13962_CR8","doi-asserted-by":"publisher","DOI":"10.3390\/biom14070788","author":"W Filipiak","year":"2024","unstructured":"Filipiak W, Wenzel M, Ager C, Mayhew CA, Bogiel T, W\u0142odarski R, Nagl M (2024) Molecular analysis of volatile metabolites synthesized by Candida albicans and Staphylococcus aureus in in vitro cultures and bronchoalveolar lavage specimens reflecting single- or duo-factor pneumonia. Biomolecules. https:\/\/doi.org\/10.3390\/biom14070788","journal-title":"Biomolecules"},{"key":"13962_CR9","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-74909-w","volume":"10","author":"S Fitzgerald","year":"2020","unstructured":"Fitzgerald S, Duffy E, Holland L, Morrin A (2020S) Multi-strain volatile profiling of pathogenic and commensal cutaneous bacteria. Sci Rep 10:17971. https:\/\/doi.org\/10.1038\/s41598-020-74909-w","journal-title":"Sci Rep"},{"key":"13962_CR10","doi-asserted-by":"publisher","unstructured":"Grandy JJ, Singh V, Lashgari M, Gauthier M, Pawliszyn J (2018)\u00a0Development of a hydrophilic lipophilic balanced thin film solid phase microextraction device for balanced determination of volatile organic compounds. Anal Chem 90:14072\u201314080.\u00a0https:\/\/doi.org\/10.1021\/acs.analchem.8b04544","DOI":"10.1021\/acs.analchem.8b04544"},{"key":"13962_CR11","doi-asserted-by":"publisher","first-page":"660","DOI":"10.1016\/J.AJIC.2017.01.024","volume":"45","author":"LP Guo","year":"2017","unstructured":"Guo LP, Li G, Wang J, Zhao X, Wang S, Zhai L, Jia H, Cao B (2017) Suspicious outbreak of ventilator-associated pneumonia caused by Burkholderia cepacia in a surgical intensive care unit. Am J Infect Control 45:660\u2013666. https:\/\/doi.org\/10.1016\/J.AJIC.2017.01.024","journal-title":"Am J Infect Control"},{"key":"13962_CR12","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-020-02290-6","volume":"10","author":"A Guti\u00e9rrez-Santa Ana","year":"2020","unstructured":"Guti\u00e9rrez-Santa Ana A, Carrillo-Cerda HA, Rodriguez-Campos J, Kirchmayr MR, Contreras-Ramos SM, Vel\u00e1zquez-Fern\u00e1ndez JB (2020A) Volatile emission compounds from plant growth-promoting bacteria are responsible for the antifungal activity against F. solani. 3 Biotech 10:292. https:\/\/doi.org\/10.1007\/s13205-020-02290-6","journal-title":"3 Biotech"},{"key":"13962_CR13","doi-asserted-by":"publisher","unstructured":"Illanad GSK, Saket S, Ghosh C (2024)\u00a0Recent advances in solid phase microextraction with various geometries in environmental analysis RSC Adv 14:27608\u201327621.\u00a0https:\/\/doi.org\/10.1039\/D4RA03251A","DOI":"10.1039\/D4RA03251A"},{"key":"13962_CR14","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2019.104319","author":"V Jalili","year":"2020","unstructured":"Jalili V, Barkhordari A, Ghiasvand A (2020V) A comprehensive look at solid-phase microextraction technique: a review of reviews. Microchem J. https:\/\/doi.org\/10.1016\/j.microc.2019.104319","journal-title":"Microchem J"},{"key":"13962_CR15","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1016\/j.trac.2012.07.005","volume":"39","author":"R Jiang","year":"2012","unstructured":"Jiang R, Pawliszyn J (2012R) Thin-film microextraction offers another geometry for solid-phase microextraction. TrAC Trends Anal Chem 39:245\u2013253","journal-title":"TrAC Trends Anal Chem"},{"key":"13962_CR16","doi-asserted-by":"publisher","unstructured":"Kabir E, Kim K-H (2012)\u00a0Use of solid phase microextraction (SPME) in the analysis of the reduced sulfur compounds (RSC) and its experimental limitations Microchem J 103:42\u201348.\u00a0https:\/\/doi.org\/10.1016\/j.microc.2012.01.005","DOI":"10.1016\/j.microc.2012.01.005"},{"key":"13962_CR17","doi-asserted-by":"publisher","unstructured":"Krumplewski W, Rykowska I (2024)\u00a0Krumplewski I Rykowska 2024 New materials for thin-film solid-phase microextraction (TF-SPME) and their use for isolation and preconcentration of selected compounds from aqueous, biological and food matrices Molecules 29 5025.\u00a0https:\/\/doi.org\/10.3390\/molecules29215025","DOI":"10.3390\/molecules29215025"},{"key":"13962_CR18","doi-asserted-by":"publisher","unstructured":"Liao CH, Chang HT, Lai CC, Huang YT, Hsu MS, Liu CY, Yang CJ, Hsueh PR (2011)\u00a0Clinical characteristics and outcomes of patients with Burkholderia cepacia bacteremia in an intensive care unit Diagn Microbiol Infect Dis 70 260 266.\u00a0https:\/\/doi.org\/10.1016\/J.DIAGMICROBIO.2011.01.008","DOI":"10.1016\/J.DIAGMICROBIO.2011.01.008"},{"key":"13962_CR19","doi-asserted-by":"publisher","unstructured":"LiPuma JJ (2010)\u00a0The changing microbial epidemiology in cystic fibrosis Clin Microbiol Rev 23:299\u2013323.\u00a0https:\/\/doi.org\/10.1128\/CMR.00068-09","DOI":"10.1128\/CMR.00068-09"},{"key":"13962_CR20","doi-asserted-by":"publisher","unstructured":"Liu C, He Z, Kong M, Jin D (2024)\u00a0Development of a duplex droplet digital PCR assay for the detection of Burkholderia cepacia complex and Stenotrophomonas maltophilia in bloodstream infections. Microbiol Spectr 12. https:\/\/doi.org\/10.1128\/spectrum.03569-23","DOI":"10.1128\/spectrum.03569-23"},{"key":"13962_CR21","doi-asserted-by":"publisher","DOI":"10.3390\/molecules31040730","author":"MFA Magalh\u00e3es","year":"2026","unstructured":"Magalh\u00e3es MFA, Martins RO, Costa JO, Alves J, Lan\u00e7as FM (2026) In-tube solid phase microextraction: basic concepts and recent applications in food matrices. Molecules. https:\/\/doi.org\/10.3390\/molecules31040730","journal-title":"Molecules"},{"key":"13962_CR22","doi-asserted-by":"publisher","first-page":"32230","DOI":"10.1039\/D4RA05907G","volume":"14","author":"D Mandal","year":"2024","unstructured":"Mandal D, Dey I, Ghosh C (2024D) Development of a disposable paper-based thin film solid-phase microextraction sampling kit to quantify ketone body. RSC Adv 14:32230\u201332238. https:\/\/doi.org\/10.1039\/D4RA05907G","journal-title":"RSC Adv"},{"key":"13962_CR23","doi-asserted-by":"publisher","first-page":"7598","DOI":"10.1039\/D3GC02347H","volume":"25","author":"N Manousi","year":"2023","unstructured":"Manousi N, Wojnowski W, P\u0142otka-Wasylka J, Samanidou V (2023N) Blue applicability grade index (BAGI) and software: a new tool for the evaluation of method practicality. Green Chem 25:7598\u20137604. https:\/\/doi.org\/10.1039\/D3GC02347H","journal-title":"Green Chem"},{"key":"13962_CR24","doi-asserted-by":"publisher","unstructured":"Meena S, Bir R, Sood S, Das BK, Kapil A (2019)\u00a0Emergence of Burkholderia cepacia in ICU setting Indian J Crit Care Med 23:423\u2013426.\u00a0https:\/\/doi.org\/10.5005\/jp-journals-10071-23237","DOI":"10.5005\/jp-journals-10071-23237"},{"key":"13962_CR25","doi-asserted-by":"publisher","DOI":"10.1016\/j.greeac.2022.100016","author":"K Murtada","year":"2022","unstructured":"Murtada K, Pawliszyn J (2022K) Protocol for the development of TFME-GC methods for analyzing multiclass organic constituents in water samples. Green Anal Chem. https:\/\/doi.org\/10.1016\/j.greeac.2022.100016","journal-title":"Green Anal Chem"},{"key":"13962_CR26","doi-asserted-by":"publisher","unstructured":"Nie Y, Luo Y, Luo S, Cao X, Song G, Deng C (2022)\u00a0Amphiphilic copolymers grafted on monodisperse magnetic microspheres as an efficient adsorbent for the extraction of safrole in the plasma J Chromatogr A 1662:46273310.\u00a0https:\/\/doi.org\/10.1016\/j.chroma.2021.462733","DOI":"10.1016\/j.chroma.2021.462733"},{"key":"13962_CR27","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.trac.2019.01.022","volume":"113","author":"YA Olcer","year":"2019","unstructured":"Olcer YA, Tascon M, Eroglu AE, Boyac\u0131 E (2019) Thin film microextraction: towards faster and more sensitive microextraction. TrAC Trends Anal Chem 113:93\u2013101","journal-title":"TrAC Trends Anal Chem"},{"key":"13962_CR28","doi-asserted-by":"publisher","unstructured":"Patra S, Bhat RY, Lewis LE, Purakayastha J, Vamsi Sivaramaraju V, Kalwaje E V, Mishra S (2014)\u00a0Burkholderia cepacia sepsis among neonates Indian J Pediatr 81:1233\u20131236.\u00a0https:\/\/doi.org\/10.1007\/s12098-014-1473-9","DOI":"10.1007\/s12098-014-1473-9"},{"key":"13962_CR29","doi-asserted-by":"publisher","unstructured":"Priyanka B, Patil RK, Dwarakanath S (2016\u00a0A review on detection methods used for foodborne pathogens Indian J Med Res 144:327\u2013338.\u00a0https:\/\/doi.org\/10.4103\/0971-5916.198677","DOI":"10.4103\/0971-5916.198677"},{"key":"13962_CR30","doi-asserted-by":"publisher","unstructured":"Reyes-Garc\u00e9s N, Bojko B, Pawliszyn J (2014)\u00a0High throughput quantification of prohibited substances in plasma using thin film solid phase microextraction J Chromatogr A\u00a01374:40\u201349.\u00a0https:\/\/doi.org\/10.1016\/j.chroma.2014.11.047","DOI":"10.1016\/j.chroma.2014.11.047"},{"key":"13962_CR31","doi-asserted-by":"crossref","unstructured":"Reyes-Garc\u00e9s N, Gionfriddo E, G\u00f3mez-R\u00edos GA, Alam MN, Boyacl E, Bojko B, Singh V, Grandy J, Pawliszyn J (2018)\u00a0Advances in solid phase microextraction and perspective on future directions. Anal Chem 90:302\u2013360","DOI":"10.1021\/acs.analchem.7b04502"},{"key":"13962_CR32","doi-asserted-by":"publisher","unstructured":"Selvamuthukumar K, Mandal D, Poojary H, Illanad G, Prasad A. S. B, Koo S, Ghosh C (2025)\u00a0Fabrication of disposable microextraction analytical tool for in vitro detection of Staphylococcus bacterial pathogen using volatile metabolites emission RSC Adv 15:11391\u201311400.\u00a0https:\/\/doi.org\/10.1039\/D4RA09099C","DOI":"10.1039\/D4RA09099C"},{"key":"13962_CR33","doi-asserted-by":"publisher","unstructured":"Shaban RZ, Sotomayor-Castillo C, Nahidi S, Li C, MacBeth D, Mitchell BG, Russo PL (2020)\u00a0Global burden, point sources, and outbreak management of healthcare-associated Burkholderia cepacia infections: an integrative review Infect Control Hosp Epidemiol 41:777\u2013783.\u00a0https:\/\/doi.org\/10.1017\/ice.2020.184","DOI":"10.1017\/ice.2020.184"},{"key":"13962_CR34","doi-asserted-by":"publisher","unstructured":"Shi H, Chen X, Chen L, Zhu B, Yan W, Ma X (2023) Burkholderia cepacia infection in children without cystic fibrosis: a clinical analysis of 50 cases. Front Pediatr 11. https:\/\/doi.org\/10.3389\/fped.2023.1115877","DOI":"10.3389\/fped.2023.1115877"},{"key":"13962_CR35","doi-asserted-by":"publisher","unstructured":"Shmarina G, Pukhalskaya D, Shmarin V, Semykin S, Avakyan L, Krasovsky S, Goryainova A, Kostyuk S, Zinchenko R, Kashirskaya N (2024) Burkholderia cepacia in cystic fibrosis children and adolescents: overall survival and immune alterations. Front Cell Infect Microbiol 14. https:\/\/doi.org\/10.3389\/fcimb.2024.1374318","DOI":"10.3389\/fcimb.2024.1374318"},{"key":"13962_CR36","doi-asserted-by":"publisher","unstructured":"Sumner LW, Amberg A, Barrett D, Beale MH, Beger R, Daykin CA, Fan TW-M, Fiehn O, Goodacre R, Griffin JL, Hankemeier T, Hardy N, Harnly J, Higashi R, Kopka J, Lane AN, Lindon JC, Marriott P, Nicholls AW, Reily MD, Thaden JJ, Viant MR (2007)\u00a0Proposed minimum reporting standards for chemical analysis Metabolomics 3:211\u2013221.\u00a0https:\/\/doi.org\/10.1007\/s11306-007-0082-2","DOI":"10.1007\/s11306-007-0082-2"},{"key":"13962_CR37","doi-asserted-by":"publisher","unstructured":"Tavares M, Kozak M, Balola A, S\u00e1-Correia I (2020) Burkholderia cepacia complex bacteria: a feared contamination risk in water-based pharmaceutical products. Clin Microbiol Rev 33. https:\/\/doi.org\/10.1128\/CMR.00139-19","DOI":"10.1128\/CMR.00139-19"},{"key":"13962_CR39","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00491","author":"CM Timm","year":"2018","unstructured":"Timm CM, Lloyd EP, Egan A, Mariner R, Karig D (2018) Direct growth of bacteria in headspace vials allows for screening of volatiles by gas chromatography mass spectrometry. Front Microbiol. https:\/\/doi.org\/10.3389\/fmicb.2018.00491","journal-title":"Front Microbiol"},{"key":"13962_CR40","doi-asserted-by":"publisher","unstructured":"van der Merwe P, Forbes P (2024)\u00a0Comparison of three sorbents for thin film solid phase microextraction of haloacetic acids from water Anal Methods 16:5154\u20135165.\u00a0https:\/\/doi.org\/10.1039\/D4AY00634H","DOI":"10.1039\/D4AY00634H"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00253-026-13962-3","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-026-13962-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-026-13962-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T01:57:51Z","timestamp":1791511071000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00253-026-13962-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,15]]},"references-count":39,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["13962"],"URL":"https:\/\/doi.org\/10.1007\/s00253-026-13962-3","relation":{},"ISSN":["1432-0614"],"issn-type":[{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,15]]},"assertion":[{"value":"30 January 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 April 2026","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 July 2026","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 July 2026","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Ethical approval was obtained from the Kasturba Medical College and Kasturba Hospital Ethics Committee. The reference number is IEC1:508\/2024.","order":1,"name":"Ethics","label":"Ethics approval","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"303"}}