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In the search of alternatives, we developed a high-yield biotechnological production process for hydroxycinnamic acid\u2013tyramine conjugates in\n                      <jats:italic>Escherichia\u00a0coli<\/jats:italic>\n                      by converting soluble substrates into insoluble products. A recombinant plant-enzyme cascade was established by co-expression of\n                      <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                      4-coumarate:CoA ligase and\n                      <jats:italic>Solanum tuberosum<\/jats:italic>\n                      hydroxycinnamoyl-CoA:tyramine\n                      <jats:italic>N<\/jats:italic>\n                      -(hydroxycinnamoyl)transferase. We overcame initial enzymatic limitations by developing a temperature adapted and sequentially phased bioprocess on a 1 L reactor scale. With 5\u00a0mM\/h substrate supply rate we achieved 31.9\u00a0g\/L (102\u00a0mM) of feruloyltyramine (FerT), 14.4\u00a0g\/L (50.7\u00a0mM) coumaroyltyramine, and 14.6\u00a0g\/L (46.5\u00a0mM) isoferuloyltyramine with a space time yield of 1.75\u00a0g\u00a0*L\n                      <jats:sup>\u22121<\/jats:sup>\n                      \u00a0*h\n                      <jats:sup>\u22121<\/jats:sup>\n                      FerT. The vast majority of the product was obtained as filterable precipitate, simplifying the downstream process compared to classical procedures. Washed filter cake from a FerT production after filtration via 16\u201340\u00a0\u00b5m glass filter, showed 99% mass content of FerT. Product identity was confirmed by NMR after recrystallization. The newly developed process is robust, cost-effective and scalable, paving the way for commercial applications of biotechnologically produced hydroxycinnamic acid\u2013tyramines.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      \u2022\n                      <jats:italic>Plant derived enzymes expressed at low temperature enable high productivity<\/jats:italic>\n                    <\/jats:p>\n                    <jats:p>\n                      \u2022\n                      <jats:italic>In situ precipitated product is readily filtered from fermentation broth<\/jats:italic>\n                    <\/jats:p>\n                    <jats:p>\n                      \u2022\n                      <jats:italic>Carbon transfer rate mirrors toxicity of phenolics and guide process optimization<\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00253-026-13997-6","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T13:05:28Z","timestamp":1787749528000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Efficient biotechnological production of N-(Hydroxycinnamoyl)tyramines by utilizing plant-derived enzymes in a bench-scale bioreactor with in situ product precipitation"],"prefix":"10.1007","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-1559-3252","authenticated-orcid":false,"given":"Israa Abuobida","family":"Mohamed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1452-7203","authenticated-orcid":false,"given":"Qais","family":"Al-Khawlani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0630-8105","authenticated-orcid":false,"given":"Raika","family":"Milde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6540-6357","authenticated-orcid":false,"given":"Niels Valentin","family":"Heise","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7123-7812","authenticated-orcid":false,"given":"Martin","family":"Dippe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7280-5153","authenticated-orcid":false,"given":"Mohamed","family":"Nagia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2060-8235","authenticated-orcid":false,"given":"Ludger A.","family":"Wessjohann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6266-9621","authenticated-orcid":false,"given":"Markus","family":"Pietzsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9339-3409","authenticated-orcid":false,"given":"Bodo","family":"Moritz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,26]]},"reference":[{"issue":"4","key":"13997_CR1","doi-asserted-by":"publisher","first-page":"385","DOI":"10.3109\/10242422.2012.701623","volume":"30","author":"MA Alrub","year":"2012","unstructured":"Alrub MA, Basri M, Malek EA, Alang Ahmad SA, Salleh AB, Abdul Rahman MB (2012) Lipase catalysed synthesis of N-trans-feruloyltyramine and a quantitative HPLC-UV method for analysis. 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