{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T23:55:26Z","timestamp":1790898926347,"version":"4.1.0"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"DOI":"10.1007\/s00253-026-14035-1","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T10:04:35Z","timestamp":1790849075000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Alluvial filtration as a scalable and safe single-use technology for the harvest of viral vectors"],"prefix":"10.1007","volume":"110","author":[{"given":"Johanna","family":"Wiesner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amelie","family":"Bauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Detlef","family":"Eisenkraetzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,1]]},"reference":[{"key":"14035_CR1","doi-asserted-by":"publisher","first-page":"3199","DOI":"10.1002\/bit.27472","volume":"117","author":"B Adams","year":"2020","unstructured":"Adams B, Bak H, Tustian AD (2020) Moving from the bench towards a large scale, industrial platform process for adeno-associated viral vector purification. Biotechnol Bioeng 117:3199\u20133211. https:\/\/doi.org\/10.1002\/bit.27472","journal-title":"Biotechnol Bioeng"},{"issue":"5","key":"14035_CR2","doi-asserted-by":"publisher","DOI":"10.3390\/v14051038","volume":"14","author":"MI Beloukhova","year":"2022","unstructured":"Beloukhova MI, Lukashev AN, Volchkov PY, Zamyatnin AA Jr, Deviatkin AA (2022) Robust AAV genotyping based on genetic distances in rep gene that are maintained by ubiquitous recombination. Viruses 14(5). https:\/\/doi.org\/10.3390\/v14051038","journal-title":"Viruses"},{"key":"14035_CR3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.biotechadv.2015.11.005","volume":"34","author":"L Besnard","year":"2016","unstructured":"Besnard L, Fabre V, Fettig M, Gousseinov E, Kawakami Y, Laroudie N, Scanlan C, Pattnaik P (2016) Clarification of vaccines: an overview of filter based technology trends and best practices. Biotechnol Adv 34:1\u201313. https:\/\/doi.org\/10.1016\/j.biotechadv.2015.11.005","journal-title":"Biotechnol Adv"},{"key":"14035_CR4","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1007\/978-1-4939-8730-6_17","volume":"1850","author":"D Blessing","year":"2018","unstructured":"Blessing D, D\u00e9glon N, Schneider BL (2018) Scalable production and purification of adeno-associated viral vectors (AAV). Methods Mol Bio 1850:259\u2013274. https:\/\/doi.org\/10.1007\/978-1-4939-8730-6_17","journal-title":"Methods Mol Bio"},{"key":"14035_CR5","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1016\/j.memsci.2005.12.057","volume":"279","author":"GR Bolton","year":"2006","unstructured":"Bolton GR, Boesch AW, Lazzara MJ (2006) The effects of flow rate on membrane capacity: development and application of adsorptive membrane fouling models. J Membr Sci 279:625\u2013634. https:\/\/doi.org\/10.1016\/j.memsci.2005.12.057","journal-title":"J Membr Sci"},{"key":"14035_CR6","doi-asserted-by":"publisher","first-page":"1552","DOI":"10.1021\/bp0500821","volume":"21","author":"MA Chandler","year":"2005","unstructured":"Chandler MA, Zydney AL (2005) Clarification of yeast cell suspensions by depth filtration. Biotechnol Prog 21:1552\u20131557. https:\/\/doi.org\/10.1021\/bp0500821","journal-title":"Biotechnol Prog"},{"key":"14035_CR7","unstructured":"Central Committee on Biological Safety (n.d.)\u00a0ZKBS according to\u00a0https:\/\/zkbs-online.de\/en\/. Accessed 24 November 2025"},{"key":"14035_CR8","doi-asserted-by":"publisher","unstructured":"Daumke R, Medvedev V, Albano T, Rousset F (2019) Alluvial filtration. In: Eibl R and Eibl D (eds) Single-use technology in biopharmaceutical manufacture. https:\/\/doi.org\/10.1002\/9781119477891.ch23","DOI":"10.1002\/9781119477891.ch23"},{"key":"14035_CR9","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1016\/j.molmed.2020.09.010","volume":"27","author":"BP Dhungel","year":"2021","unstructured":"Dhungel BP, Bailey CG, Rasko JEJ (2021) Journey to the center of the cell: tracing the path of AAV transduction. Trends Mol Med 27:172\u2013184. https:\/\/doi.org\/10.1016\/j.molmed.2020.09.010","journal-title":"Trends Mol Med"},{"key":"14035_CR10","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2020.107892","volume":"167","author":"WA Dryden","year":"2021","unstructured":"Dryden WA, Larsen LM, Britt DW, Smith MT (2021) Technical and economic considerations of cell culture harvest and clarification technologies. Biochem Eng J 167. https:\/\/doi.org\/10.1016\/j.bej.2020.107892","journal-title":"Biochem Eng J"},{"key":"14035_CR11","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.3721","volume":"26","author":"SL Ginn","year":"2024","unstructured":"Ginn SL, Mandwie M, Alexander IE, Edelstein M, Abedi MR (2024) Gene therapy clinical trials worldwide to 2023-an update. J Gene Med 26. https:\/\/doi.org\/10.1002\/jgm.3721","journal-title":"J Gene Med"},{"key":"14035_CR12","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1089\/gen.38.05.07","volume":"38","author":"M Grauf","year":"2018","unstructured":"Grauf M, Lagrange B, Sch\u00f6ne K (2018) Simplified small-scale harvest of CHO cells for mAb analytics. Genet Eng Biotechnol News 38:22\u201323. https:\/\/doi.org\/10.1089\/gen.38.05.07","journal-title":"Genet Eng Biotechnol News"},{"key":"14035_CR13","doi-asserted-by":"publisher","unstructured":"Gupta A, Amara JP, Gousseinov E, Cacace B (2020) Chapter 6 - Recent advances in harvest clarification for antibodies and related products. In: Matte A (ed) Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics. Elsevier, Amsterdam, pp 117\u2013136. https:\/\/doi.org\/10.1016\/B978-0-08-103019-6.00006-0","DOI":"10.1016\/B978-0-08-103019-6.00006-0"},{"key":"14035_CR14","doi-asserted-by":"publisher","first-page":"131","DOI":"10.18609\/cgti.2018.016","volume":"4","author":"M Hebben","year":"2018","unstructured":"Hebben M (2018) Downstream bioprocessing of AAV vectors: industrial challenges & regulatory requirements. Cell Gene Ther Insights 4:131\u2013146. https:\/\/doi.org\/10.18609\/cgti.2018.016","journal-title":"Cell Gene Ther Insights"},{"key":"14035_CR15","doi-asserted-by":"publisher","DOI":"10.3390\/cells12050785","volume":"12","author":"SS Issa","year":"2023","unstructured":"Issa SS, Shaimardanova AA, Solovyeva VV, Rizvanov AA (2023) Various AAV serotypes and their applications in gene therapy: an overview. Cells 12. https:\/\/doi.org\/10.3390\/cells12050785","journal-title":"Cells"},{"key":"14035_CR16","doi-asserted-by":"publisher","first-page":"1938","DOI":"10.1002\/bit.26707","volume":"115","author":"O Khanal","year":"2018","unstructured":"Khanal O, Singh N, Traylor SJ, Xu X, Ghose S, Li ZJ, Lenhoff AM (2018) Contributions of depth filter components to protein adsorption in bioprocessing. Biotechnol Bioeng 115:1938\u20131948. https:\/\/doi.org\/10.1002\/bit.26707","journal-title":"Biotechnol Bioeng"},{"key":"14035_CR17","doi-asserted-by":"publisher","first-page":"464","DOI":"10.1016\/j.memsci.2018.10.058","volume":"570","author":"O Khanal","year":"2019","unstructured":"Khanal O, Xu X, Singh N, Traylor SJ, Huang C, Ghose S, Li ZJ, Lenhoff AM (2019) DNA retention on depth filters. J Memb Sci 570:464\u2013471. https:\/\/doi.org\/10.1016\/j.memsci.2018.10.058","journal-title":"J Memb Sci"},{"key":"14035_CR18","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.jbiotec.2020.12.004","volume":"326","author":"JJ Labisch","year":"2021","unstructured":"Labisch JJ, Bollmann F, Wolff MW, Pflanz K (2021) A new simplified clarification approach for lentiviral vectors using diatomaceous earth improves throughput and safe handling. J Biotechnol 326:11\u201320. https:\/\/doi.org\/10.1016\/j.jbiotec.2020.12.004","journal-title":"J Biotechnol"},{"key":"14035_CR19","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24097736","volume":"24","author":"X Li","year":"2023","unstructured":"Li X, Le Y, Zhang Z, Nian X, Liu B, Yang X (2023) Viral vector-based gene therapy. Int J Mol Sci 24. https:\/\/doi.org\/10.3390\/ijms24097736","journal-title":"Int J Mol Sci"},{"key":"14035_CR20","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/j.jbiotec.2023.03.010","volume":"367","author":"F Meierrieks","year":"2023","unstructured":"Meierrieks F, Pickl A, Wolff MW (2023) A robust and efficient alluvial filtration method for the clarification of adeno-associated viruses from crude cell lysates. J Biotechnol 367:31\u201341. https:\/\/doi.org\/10.1016\/j.jbiotec.2023.03.010","journal-title":"J Biotechnol"},{"key":"14035_CR21","doi-asserted-by":"publisher","first-page":"464","DOI":"10.1016\/j.ymthe.2020.12.007","volume":"29","author":"JR Mendell","year":"2021","unstructured":"Mendell JR, Al-Zaidy SA, Rodino-Klapac LR, Goodspeed K, Gray SJ, Kay CN, Boye SL, Boye SE, George LA, Salabarria S, Corti M, Byrne BJ, Tremblay JP (2021) Current clinical applications of in vivo gene therapy with AAVs. Mol Ther 29:464\u2013488. https:\/\/doi.org\/10.1016\/j.ymthe.2020.12.007","journal-title":"Mol Ther"},{"key":"14035_CR22","doi-asserted-by":"publisher","DOI":"10.1016\/j.coche.2021.100746","volume":"34","author":"N Nejatishahidein","year":"2021","unstructured":"Nejatishahidein N, Zydney AL (2021) Depth filtration in bioprocessing \u2014 new opportunities for an old technology. Curr Opin Chem Eng 34. https:\/\/doi.org\/10.1016\/j.coche.2021.100746","journal-title":"Curr Opin Chem Eng"},{"key":"14035_CR23","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.3028","volume":"36","author":"N Nejatishahidein","year":"2020","unstructured":"Nejatishahidein N, Borujeni EE, Roush DJ, Zydney AL (2020) Effectiveness of host cell protein removal using depth filtration with a filter containing diatomaceous earth. Biotechnol Prog 36. https:\/\/doi.org\/10.1002\/btpr.3028","journal-title":"Biotechnol Prog"},{"key":"14035_CR24","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201700771","volume":"14","author":"HC Nguyen","year":"2019","unstructured":"Nguyen HC, Langland AL, Amara JP, Dullen M, Kahn DS, Costanzo JA (2019) Improved HCP reduction using a new, all-synthetic depth filtration media within an antibody purification process. Biotechnol J 14. https:\/\/doi.org\/10.1002\/biot.201700771","journal-title":"Biotechnol J"},{"key":"14035_CR25","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1146\/annurev-chembioeng-092220-030223","volume":"13","author":"JJ Samaras","year":"2022","unstructured":"Samaras JJ, Micheletti M, Ding W (2022) Transformation of biopharmaceutical manufacturing through single-use technologies: current state, remaining challenges, and future development. Annu Rev Chem Biomol Eng 13:73\u201397. https:\/\/doi.org\/10.1146\/annurev-chembioeng-092220-030223","journal-title":"Annu Rev Chem Biomol Eng"},{"key":"14035_CR26","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2022.120375","volume":"294","author":"N Sayed","year":"2022","unstructured":"Sayed N, Allawadhi P, Khurana A, Singh V, Navik U, Pasumarthi SK, Khurana I, Banothu AK, Weiskirchen R, Bharani KK (2022) Gene therapy: comprehensive overview and therapeutic applications. Life Sci 294. https:\/\/doi.org\/10.1016\/j.lfs.2022.120375","journal-title":"Life Sci"},{"key":"14035_CR27","doi-asserted-by":"publisher","first-page":"312","DOI":"10.2533\/chimia.2025.312","volume":"79","author":"DR Schmidhalter","year":"2025","unstructured":"Schmidhalter DR, Eibl D, Eibl R (2025) Single-use technology today - a cornucopia of applications. Chimia (Aarau) 79:312\u2013323. https:\/\/doi.org\/10.2533\/chimia.2025.312","journal-title":"Chimia (Aarau)"},{"key":"14035_CR28","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1007\/978-1-61779-095-9_4","volume":"737","author":"MM Segura","year":"2011","unstructured":"Segura MM, Kamen AA, Garnier A (2011) Overview of current scalable methods for purification of viral vectors. Methods Mol Biol 737:89\u2013116. https:\/\/doi.org\/10.1007\/978-1-61779-095-9_4","journal-title":"Methods Mol Biol"},{"key":"14035_CR29","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1016\/j.tibtech.2012.10.004","volume":"31","author":"AA Shukla","year":"2013","unstructured":"Shukla AA, Gottschalk U (2013) Single-use disposable technologies for biopharmaceutical manufacturing. Trends Biotechnol 31:147\u2013154. https:\/\/doi.org\/10.1016\/j.tibtech.2012.10.004","journal-title":"Trends Biotechnol"},{"key":"14035_CR30","doi-asserted-by":"publisher","first-page":"2609","DOI":"10.1016\/j.xphs.2021.03.024","volume":"110","author":"A Srivastava","year":"2021","unstructured":"Srivastava A, Mallela KMG, Deorkar N, Brophy G (2021) Manufacturing challenges and rational formulation development for AAV viral vectors. J Pharm Sci 110:2609\u20132624. https:\/\/doi.org\/10.1016\/j.xphs.2021.03.024","journal-title":"J Pharm Sci"},{"key":"14035_CR31","first-page":"25","volume":"12","author":"T van der Meer","year":"2014","unstructured":"van der Meer T, Minow B, Lagrange B, Krumbein F, Rolin F (2014) Diatomaceous Earth filtration: innovative single-use concepts for clarification of high-density mammalian cell cultures. Bioprocess Int 12:25\u201328","journal-title":"Bioprocess Int"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00253-026-14035-1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-026-14035-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-026-14035-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T23:23:18Z","timestamp":1790896998000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00253-026-14035-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,1]]},"references-count":31,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["14035"],"URL":"https:\/\/doi.org\/10.1007\/s00253-026-14035-1","relation":{},"ISSN":["1432-0614"],"issn-type":[{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,1]]},"assertion":[{"value":"22 June 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 August 2026","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 September 2026","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 October 2026","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Not applicable.","order":1,"name":"Ethics","label":"Ethical approval","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"J.W. (Principal Associate), A.B. (Senior Scientist), and D.E. (Senior Principal Scientist) are employed at Roche Diagnostics GmbH in the PTCG department.","order":2,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"293"}}