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To facilitate this, we developed a highly efficient no-wash red optical molecular imaging agent that enables the rapid, selective, and specific visualization of Gram-positive bacteria through a bespoke optical fiber\u2013based delivery\/imaging endoscopic device.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We rationally designed a no-wash, red, Gram-positive-specific molecular imaging agent (Merocy-Van) based on vancomycin and an environmental merocyanine dye. We demonstrated the specificity and utility of the imaging agent in escalating in vitro and ex vivo whole human lung models (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20093), utilizing a bespoke fiber\u2013based delivery and imaging device, coupled to a wide-field, two-color endomicroscopy system.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The imaging agent (Merocy-Van) was specific to Gram-positive bacteria and enabled no-wash imaging of\n                      <jats:italic>S. aureus<\/jats:italic>\n                      within the alveolar space of whole ex vivo human lungs within 60\u00a0s of delivery into the field-of-view, using the novel imaging\/delivery endomicroscopy device.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This platform enables the rapid and specific detection of Gram-positive bacteria in the human lung.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00259-020-05021-4","type":"journal-article","created":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T10:03:22Z","timestamp":1599818602000},"page":"800-807","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Molecular detection of Gram-positive bacteria in the human lung through an optical fiber\u2013based endoscope"],"prefix":"10.1007","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3209-9490","authenticated-orcid":false,"given":"Bethany","family":"Mills","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1373-8675","authenticated-orcid":false,"given":"Alicia","family":"Megia-Fernandez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominic","family":"Norberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheelagh","family":"Duncan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Marshall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4605-1682","authenticated-orcid":false,"given":"Ahsan R.","family":"Akram","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Quinn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Young","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5790-5802","authenticated-orcid":false,"given":"Annya M.","family":"Bruce","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Scholefield","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gareth O. 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