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Multiple linear regression analyses were performed to identify independent predictors of rest MBF (rMBF), stress MBF (sMBF), and myocardial flow reserve (MFR), and predictor importance was calculated. Finally, median, LRL, and URL for rMBF, sMBF, and MFR were calculated based on the presence of predictors.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Among 784 patients with a median coronary artery calcium score (CACS) of 69, median rMBF was 0.75mL\u2219min<jats:sup>\u2212\u20091<\/jats:sup>\u2219g<jats:sup>\u2212\u20091<\/jats:sup> (LRL\u2009=\u20090.49 mL\u2219min<jats:sup>\u2212\u20091<\/jats:sup>\u2219g<jats:sup>\u2212\u20091<\/jats:sup>; URL\u2009=\u20091.33 mL\u2219min<jats:sup>\u2212\u20091<\/jats:sup>\u2219g<jats:sup>\u2212\u20091<\/jats:sup>), median sMBF was 2.41mL\u2219min<jats:sup>\u2212\u20091<\/jats:sup>\u2219g<jats:sup>\u2212\u20091<\/jats:sup> (LRL\u2009=\u20091.42 mL\u2219min<jats:sup>\u2212\u20091<\/jats:sup>\u2219g<jats:sup>\u2212\u20091<\/jats:sup>; URL\u2009=\u20093.73 mL\u2219min<jats:sup>\u2212\u20091<\/jats:sup>\u2219g<jats:sup>\u2212\u20091<\/jats:sup>), and median MFR was 3.09 (LRL\u2009=\u20092.11; URL\u2009=\u20094.65). The body mass index (BMI) was the single most important independent predictor of rMBF, sMBF, and MFR (predictor importance of 72%, 87%, and 41%, respectively; standardized \u03b2=-0.434, -0.566 and \u2212\u20090.174, respectively). Additional predictors were sex and hypertension for rMBF, sex for sMBF, and hypertension and CACS for MFR.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>In patients without flow-limiting CAD, MBF is strongly influenced by the patient\u2019s habitus, whereby median and reference limits for sMBF and rMBF decrease with increasing BMI. Consequently, MFR exhibits stable lower reference limits across a wide range of BMI and may be considered the most robust quantitative parameter derived from 13N-ammonia PET-MPI.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s00259-025-07196-0","type":"journal-article","created":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T00:53:31Z","timestamp":1741913611000},"page":"3353-3363","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Myocardial blood flow reference values for 13N-ammonia PET myocardial perfusion imaging in patients without flow-limiting coronary artery disease"],"prefix":"10.1007","volume":"52","author":[{"given":"Rita","family":"Pingree","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Markendorf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitrios","family":"Moysidis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Ryffel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Stuetz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raffael","family":"Ghenzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marko","family":"Gajic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominik C.","family":"Benz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aju P.","family":"Pazhenkottil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas A.","family":"Giannopoulos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp A.","family":"Kaufmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Winther","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8064-8904","authenticated-orcid":false,"given":"Ronny R.","family":"Buechel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,3,14]]},"reference":[{"issue":"35","key":"7196_CR1","doi-asserted-by":"publisher","first-page":"3322","DOI":"10.1093\/eurheartj\/ehy267","volume":"39","author":"J Knuuti","year":"2018","unstructured":"Knuuti J, Ballo H, Juarez-Orozco LE, Saraste A, Kolh P, Rutjes AWS, et al. 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