{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T02:59:42Z","timestamp":1777949982914,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,11,30]],"date-time":"2006-11-30T00:00:00Z","timestamp":1164844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["World j. surg."],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>The aim of this study was to determine the effect of immunoregulatory cytokine interleukin\u201010 (IL\u201010) gene therapy on multiple organ injury (MOI) induced by a cecal ligation and puncture (CLP) model of sepsis in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Male Balb\/c mice subjected to CLP were treated with either an hIL\u201010\u2010carrying vector or an empty control vector. We assessed the degree of lung, liver, and kidney tissue destruction biochemically by measuring myeloperoxidase (MPO) and malondialdehyde (MDA) activity. Histologic assessments were based on neutrophil infiltration in lung and liver tissue. IL\u201010 protein expression was examined immunohistochemically, and ultrastructural changes in the liver were studied by transmission electron microscopy. We analyzed the expression of tumor necrosis factor\u2010\u03b1 (TNF\u03b1) mRNA by reverse transcription polymerase chain reaction 3, 8, and 24 hours after CLP in all organs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Organ damage was significantly reduced by hIL\u201010 gene transfer, which was associated at the tissue level with reduced MPO activity in the liver, lung, and kidney and decreased leukocyte sequestration and MDA formation in the lung. The liver MDA was not significantly higher in the hIL\u201010 gene therapy group than in the controls and seemed not to be affected by hIL\u201010 gene transfer. The reduced portal tract neutrophilic infiltration and preserved ultrastructure of the hepatocytes also showed that tissue function was not impaired. The lung and kidney TNF\u03b1 mRNA expression was suppressed markedly in the hIL\u201010 gene therapy group, but liver TNF\u03b1 mRNA expression varied over time.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings showed that IL\u201010 gene therapy significantly attenuated sepsis\u2010induced MOI.<\/jats:p><\/jats:sec>","DOI":"10.1007\/s00268-006-0066-9","type":"journal-article","created":{"date-parts":[[2006,12,14]],"date-time":"2006-12-14T02:57:29Z","timestamp":1166065049000},"page":"105-115","source":"Crossref","is-referenced-by-count":15,"title":["Interleukin\u201010 Gene Transfer: Prevention of Multiple Organ Injury in a Murine Cecal Ligation and Puncture Model of Sepsis"],"prefix":"10.1002","volume":"31","author":[{"given":"Burhan","family":"Kabay","sequence":"first","affiliation":[{"name":"Department of General Surgery Pamukkale University, School of Medicine Kinikli; Kamp\u00fcs\u00fc A408 2000 Denizli Turkey"},{"name":"Tekinevler Sitesi A4 Blok 23. sk. K:6 d:19 Yenisehir Denizli Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cetin","family":"Kocaefe","sequence":"additional","affiliation":[{"name":"Department of Medical Biology Hacettepe University, School of Medicine 06100 Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atac","family":"Baykal","sequence":"additional","affiliation":[{"name":"Department of General Surgery Hacettepe University, School of Medicine 06100 Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hilmi","family":"\u00d6zden","sequence":"additional","affiliation":[{"name":"Department of Anatomy Osmangazi University, School of Medicine 26480 Eskisehir Meselik Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cengiz","family":"Baycu","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology Osmangazi University, School of Medicine 26480 Eskisehir Meselik Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zafer","family":"Oner","sequence":"additional","affiliation":[{"name":"Department of General Surgery Hacettepe University, School of Medicine 06100 Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meral","family":"\u00d6zg\u00fc\u00e7","sequence":"additional","affiliation":[{"name":"Department of Medical Biology Hacettepe University, School of Medicine 06100 Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iskender","family":"Sayek","sequence":"additional","affiliation":[{"name":"Department of General Surgery Hacettepe University, School of Medicine 06100 Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,11,30]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-120-9-199405010-00009"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1491"},{"key":"e_1_2_6_4_2","first-page":"413","article-title":"Cytokines and anticytokines in the pathogenesis of sepsis","volume":"13","author":"Poll T","year":"1999","journal-title":"Surg Clin North Am"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.177.2.547"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.6.2207"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830240524"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.174.5.1209"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.157.8.3672"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s005990050036"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000057843.47705.E8"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102168.2001.01737.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510310312"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003\u20102697(78)90342\u20101"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/00024382\u2010199409000\u201000011"},{"key":"e_1_2_6_17_2","first-page":"5367","article-title":"Endogenous IL\u201010 protects mice from death during septic peritonitis","volume":"155","author":"Poll T","year":"1995","journal-title":"J Immunol"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.8.4441\u20104446.2002"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003246\u2010199805000\u201000025"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.2.699.bloodjournal872699"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.154.10.5492"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.113.5.981"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.1999.1500"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7687073"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevTherDrugCarrierSyst.v21.i4.10"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.2174\/0929867033457430"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3300455"},{"key":"e_1_2_6_28_2","first-page":"2766","article-title":"Phase I study of direct intralesional gene transfer of HLA\u2010B7 into metastatic renal carcinoma lesions","volume":"5","author":"Rini BI","year":"1999","journal-title":"Clin Cancer Res"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349\u20107006.2004.tb02194.x"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1089\/10430340360535797"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1097\/00024382\u2010199801000\u201000001"},{"key":"e_1_2_6_32_2","first-page":"923","article-title":"Animal models for intra\u2010abdominal infection","volume":"44","author":"Sayek I","year":"1997","journal-title":"Hepatogastroenterology"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003246\u2010200008000\u201000001"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.181338198"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1007\/s005950050103"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.1980.01380020006003"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1136\/thx.52.5.442"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1996.270.5.R927"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000658\u2010199108000\u201000008"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1097\/00024382\u2010199605000\u201000003"},{"key":"e_1_2_6_41_2","first-page":"624","article-title":"Endothelin\u20101 induces direct constriction of hepatic sinusoids","volume":"266","author":"Zhang JX","year":"1994","journal-title":"Am J Physiol"}],"container-title":["World Journal of Surgery"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00268-006-0066-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00268-006-0066-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1007\/s00268-006-0066-9","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T11:10:30Z","timestamp":1703416230000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1007\/s00268-006-0066-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,11,30]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,1]]}},"alternative-id":["10.1007\/s00268-006-0066-9"],"URL":"https:\/\/doi.org\/10.1007\/s00268-006-0066-9","archive":["Portico"],"relation":{},"ISSN":["0364-2313","1432-2323"],"issn-type":[{"value":"0364-2313","type":"print"},{"value":"1432-2323","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,11,30]]}}}