{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,23]],"date-time":"2024-06-23T21:52:30Z","timestamp":1719179550396},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,11,29]],"date-time":"2009-11-29T00:00:00Z","timestamp":1259452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["World j. surg."],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Primary hyperparathyroidism (pHPT) is caused by a single monoclonal adenoma in more than 80% of patients. Biomolecular mechanisms causing pHPT are still not completely known, even if a great amount of studies have been developed recently, mainly regarding angiogenesis and growth factors. Among the latter, insulin\u2010like growth factor 1 (IGF\u20101), basic fibroblastic growth factor (bFGF), vascular endothelial growth factor (VEGF), and transforming growth factor beta 1 (TGF\u2010\u03b21) and their effects have been extensively evaluated in different kinds of endocrine disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Parathyroid cell cultures were prepared from six human adenomatous parathyroid glands that were surgically removed. After 7\u00a0days of culture, the cells were refed with DMEM supplemented with 2% FCS alone (control group), or containing hrTGF\u03b21, or hrIGF\u2010I, or hrbFGF, or hrVEGF. Then, after 48\u2010hour incubation, cell count was performed by a particle count and size analyzer, and prevalence of cell cycle was analyzed by using a flow cytometer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cell count (\u00d710000) in the control group was 3.73 \u00b1 0.32. Low\u2010dose TGF\u2010\u03b21 stimulation resulted in 5.25 \u00b1 0.38 cells, and high\u2010dose TGF\u2010\u03b21 stimulation resulted in 2.35 \u00b1 0.37 cells. IGF\u20101 stimulation resulted in 5.4 \u00b1 0.65 cells, bFGF stimulation in 5.68 \u00b1 0.86 cells, and VEGF stimulation resulted in 6.03 \u00b1 1.03 cells. Statistical analysis revealed significant differences in the control group compared with the growth factor\u2010stimulated groups. Cytometry showed different results in the percentage of cells in S\u2010phase, in particular 22.65 \u00b1 4.98% of IGF\u20101\u2010stimulated cells were found in S\u2010phase compared with 7.55 \u00b1 3.2% of control group cells (<jats:italic>p<\/jats:italic> &lt; 0.0001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Growth factors seem to play an important role in parathyroid adenoma cell proliferation; IGF\u20101, bFGF, VEGF, and low\u2010dose TGF\u2010\u03b21 promote cell proliferation, whereas high\u2010dose TGF\u2010\u03b21\u00a0inhibits these phenomena.<\/jats:p><\/jats:sec>","DOI":"10.1007\/s00268-009-0294-x","type":"journal-article","created":{"date-parts":[[2009,11,28]],"date-time":"2009-11-28T16:21:41Z","timestamp":1259425301000},"page":"48-54","source":"Crossref","is-referenced-by-count":5,"title":["Role of Growth Factors on Human Parathyroid Adenoma Cell Proliferation"],"prefix":"10.1002","volume":"34","author":[{"given":"Giuseppe","family":"Cavallaro","sequence":"first","affiliation":[{"name":"Department of Surgery \u201cP. Valdoni,\u201d Policlinico Umberto I Sapienza University Viale del Policlinico 00161 Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Cucina","sequence":"additional","affiliation":[{"name":"Department of Surgery \u201cP. Valdoni,\u201d Policlinico Umberto I Sapienza University Viale del Policlinico 00161 Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierpaolo","family":"Coluccia","sequence":"additional","affiliation":[{"name":"Department of Surgery \u201cP. 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