{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T08:32:05Z","timestamp":1792225925672,"version":"4.4.0"},"reference-count":36,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2010,7,13]],"date-time":"2010-07-13T00:00:00Z","timestamp":1278979200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005071","name":"Shiraz University","doi-asserted-by":"publisher","award":["87\u2010GR\u2010VT\u201024"],"award-info":[{"award-number":["87\u2010GR\u2010VT\u201024"]}],"id":[{"id":"10.13039\/501100005071","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["World j. surg."],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Surgery is still the main treatment for hydatid disease. Recurrence of the infection is one of the end points of surgery in treating the hydatid cyst which results from the dissemination of protoscolices\u2010rich fluid. Installation of a scolicidal agent into the cyst is the most commonly employed measure to prevent recurrence. Many scolicidal agents have been used for inactivation of the cyst\u2019s content, but most of them are not safe due to their undesired side effects. In the present study, the scolicidal effect of methanolic extract of <jats:italic>Allium sativum<\/jats:italic> is investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Protoscolices were aseptically collected from sheep livers containing hydatid cysts. Two concentrations (25 and 50\u00a0mg ml<jats:sup>\u22121<\/jats:sup>) of garlic extract were used for 10, 20, 30, 40, 50, and 60\u00a0min. Viability of protoscolices was confirmed by 0.1% eosin staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>Allium sativum<\/jats:italic> extract at the concentration of 25\u00a0mg ml<jats:sup>\u22121<\/jats:sup> killed 87.9, 95.6, 96.8, 98.7, 99.6, and 100% of protoscolices following 10, 20, 30, 40, 50, and 60\u00a0min of application, respectively. Moreover, the scolicidal activity of <jats:italic>Allium sativum<\/jats:italic> extract at the concentration of 50\u00a0mg ml<jats:sup>\u22121<\/jats:sup> was 100% after 10\u00a0min of application.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Methanolic extract of <jats:italic>Allium sativum<\/jats:italic> had a high scolicidal activity in vitro and thus might be used as a scolicidal agent in the surgical treatment of the hydatid cyst. However, further investigation on the in vivo efficacy of <jats:italic>Allium sativum<\/jats:italic> extract and its possible side effects is proposed.<\/jats:p><\/jats:sec>","DOI":"10.1007\/s00268-010-0718-7","type":"journal-article","created":{"date-parts":[[2010,7,12]],"date-time":"2010-07-12T16:45:53Z","timestamp":1278953153000},"page":"2677-2681","source":"Crossref","is-referenced-by-count":72,"title":["In vitro Effectiveness of Garlic (<i>Allium sativum<\/i>) Extract on Scolices of Hydatid Cyst"],"prefix":"10.1002","volume":"34","author":[{"given":"Mohammad","family":"Moazeni","sequence":"first","affiliation":[{"name":"Department of Pathobiology Shiraz University Shiraz Fars Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Nazer","sequence":"additional","affiliation":[{"name":"Department of Pathobiology Shiraz University Shiraz Fars 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