{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T15:44:02Z","timestamp":1769010242712,"version":"3.49.0"},"reference-count":24,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T00:00:00Z","timestamp":1696982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T00:00:00Z","timestamp":1696982400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T00:00:00Z","timestamp":1696982400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["World j. surg."],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Anal fistulae are common, predominantly cryptoglandular, and almost invariably require surgical treatment. Recurrences are common for procedures other than fistulotomy regardless of technique and adequacy of repair. Growing evidence supports the pivotal role of specific intestinal bacteria in anastomotic failures after bowel resection. Anal crypts harbor colonic microbiota suggesting that similar mechanisms to anastomotic healing might prevail after anal fistula repair and hence influence healing. This study aims at assessing the potential role of the intestinal microbiome in the clinical outcomes after surgical repair of cryptoglandular anal fistula.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This is a pilot prospective cohort study enrolling patients with anal fistula undergoing endoanal advancement flap. For microbiome analysis, stool samples are taken via rectal swab before the procedure; additionally, a portion of the fistula is collected intraoperatively after fistulectomy. Samples from groups with treatment failure are compared to samples from patients who healed after surgical repair. Alpha and beta diversities and differential abundance of microbial taxa are determined and compared between groups with DADA2 analytical pipeline.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Five patients have been enrolled to date (one female, four male). At median follow\u2010up of 6\u00a0months (2\u201311), one patient experienced disease recurrence at 3\u00a0months. DNA from the 5 rectal swab and tissue samples was extracted, showing increased relative abundance of <jats:italic>Enterococcus faecalis<\/jats:italic> in samples from the patient who developed a recurrent fistula but not in those without recurrence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These very preliminary data suggest that intestinal microbiome may represent a crucial determinant of the surgical outcomes after anal fistula surgery.<\/jats:p><\/jats:sec>","DOI":"10.1007\/s00268-023-07212-0","type":"journal-article","created":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T16:01:50Z","timestamp":1697040110000},"page":"3373-3379","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Role of Microbiome in the Outcomes Following Surgical Repair of Perianal Fistula: Prospective Cohort Study Design and Preliminary Results"],"prefix":"10.1002","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6074-5982","authenticated-orcid":false,"given":"Marco","family":"Bertucci Zoccali","sequence":"first","affiliation":[{"name":"Division of Colorectal Surgery Department of Surgery New York Presbyterian\/Columbia University Medical Center New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dalia H.","family":"Moallem","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Medicine New York Presbyterian\/Columbia University Medical Center New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heekuk","family":"Park","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Medicine New York Presbyterian\/Columbia University Medical Center New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne\u2010Catrin","family":"Uhlemann","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Medicine New York Presbyterian\/Columbia University Medical Center New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James M.","family":"Church","sequence":"additional","affiliation":[{"name":"Division of Colorectal Surgery Department of Surgery New York Presbyterian\/Columbia University Medical Center New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ravi P.","family":"Kiran","sequence":"additional","affiliation":[{"name":"Division of Colorectal Surgery Department of Surgery New York Presbyterian\/Columbia University Medical Center New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,10,11]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"219","article-title":"Fistula\u2010in\u2010ano in a defined population. 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