{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T20:26:33Z","timestamp":1758399993885},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,2,24]],"date-time":"2014-02-24T00:00:00Z","timestamp":1393200000000},"content-version":"vor","delay-in-days":4771,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["World j. surg."],"published-print":{"date-parts":[[2001,2]]},"abstract":"<jats:title>Abstract.<\/jats:title><jats:p>Sucrose has been used to treat wounds with excellent results and with minimal abnormal scarring. In this study the effects of sucrose on collagen metabolism in fibroblast culture was evaluated. Sucrose (5.5, 15, or 25\u00a0mM) was added to granulation tissue, hypertrophic scar, and keloid fibroblast cultures. mRNA levels and procollagen aminopropeptides for type I and III collagens in cell culture medium were studied. Sucrose decreased mRNA levels for pro\u03b11(I) and pro\u03b11(III) collagens in fibroblast cultures derived from hypertrophic scar and keloid. In normal granulation tissue fibroblast cultures, 5.5\u00a0mM sucrose increased mRNA levels for pro\u03b11(I) and pro\u03b11(III) collagen, and higher concentrations decreased them. The synthesis of type I collagen decreased dose\u2010dependently in all cell strains, whereas the synthesis of type III collagen decreased only in granulation tissue fibroblasts. To conclude, in vitro high concentrations of sucrose down\u2010regulate both collagen gene expression and synthesis in normal granulation tissue fibroblasts, whereas in fibroblasts derived from abnormal scar sucrose down\u2010regulates only type I collagen gene expression and synthesis, changing the pattern of collagen metabolism toward normal.<\/jats:p>","DOI":"10.1007\/s002680020038","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T05:57:49Z","timestamp":1030255069000},"page":"142-146","source":"Crossref","is-referenced-by-count":6,"title":["Effect of Sucrose on Collagen Metabolism in Keloid, Hypertrophic Scar, and Granulation Tissue Fibroblast Cultures"],"prefix":"10.1002","volume":"25","author":[{"given":"Jyrki","family":"K\u00f6ssi","sequence":"first","affiliation":[{"name":"Department of Surgery University of Turku Kiinamyllynkatu 4\u20108, FIN\u201020520 Turku Finland<!--<country1>FI<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marko","family":"V\u00e4h\u00e4\u2010Kreula","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry University of Turku Kiinamyllynkatu 4\u20108 FIN\u201020520 Turku Finland<!--<country1>FI<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juha","family":"Peltonen","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry University of Turku Kiinamyllynkatu 4\u20108 FIN\u201020520 Turku Finland<!--<country1>FI<\/country1>-->"},{"name":"Department of Anatomy University of Oulu Kajaanintie 50\u201052 FIN\u201090220 Oulu Finland<!--<country1>FI<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juha","family":"Risteli","sequence":"additional","affiliation":[{"name":"Collagen Research Unit Oulu Technology Center Kajaanintie 50\u201052 FIN\u201090220 Oulu Finland<!--<country1>FI<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matti","family":"Laato","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Turku Kiinamyllynkatu 4\u20108, FIN\u201020520 Turku Finland<!--<country1>FI<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,2,24]]},"container-title":["World Journal of Surgery"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s002680020038.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s002680020038\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1007\/s002680020038","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T19:37:50Z","timestamp":1703705870000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1007\/s002680020038"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2001,2]]}},"alternative-id":["10.1007\/s002680020038"],"URL":"https:\/\/doi.org\/10.1007\/s002680020038","archive":["Portico"],"relation":{},"ISSN":["0364-2313","1432-2323"],"issn-type":[{"value":"0364-2313","type":"print"},{"value":"1432-2323","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,2]]}}}