{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T00:03:17Z","timestamp":1705017797962},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1996,3,1]],"date-time":"1996-03-01T00:00:00Z","timestamp":825638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["World j. surg."],"published-print":{"date-parts":[[1996,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The proliferative activity of epithelial cells in hyperfunctioning parathyroid glands was estimated by flow cytometric and immunohistochemical procedures. A total of 30 parathyroid glands, 29 hyperfunctioning glands, and 1\u00a0normal gland were studied. The pathology of the 29 glands was determined to be hyperplasia in 19 and adenoma in 10. The S\u2010phase cell population was expressed in terms of the S\u2010phase fraction (%SPF) calculated from the histogram by DNA flow cytometry and in terms of the BrdU immunostaining labeling index (BrdU LI). Cells in all stages of the cell cycle were studied by Ki\u201067 immunostaining and expressed in terms of the labeling index (Ki\u201067\u00a0LI). Both BrdU LI and Ki\u201067\u00a0LI values were low, ranging from 0% to 0.58% and from 0.21% to 2.62%, respectively. The BrdU LIs were lower than the Ki\u201067\u00a0LIs, ranging from one\u2010sixth to one\u2010twelfth of the values depending on the disease. There were significant correlations between the two indices (<jats:italic>p<\/jats:italic>&lt; 0.001). The %SPF determined by flow cytometry was consistently higher than both the BrdU LI and the Ki\u201067\u00a0LI. This discrepancy cannot be explained precisely, and further improvements are required for the flow cytometric analysis of %SPF. The cell cycle study by BrdU and Ki\u201067 immunohistochemistry suggested that the glandular cells of the hyperfunctioning parathyroid were characterized by low proliferative activity. No evidence of rapid cell turnover rate assumed from the flow cytometric study could be observed in the hyperfunctioning parathyroid gland.<\/jats:p>","DOI":"10.1007\/s002689900059","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T05:57:49Z","timestamp":1030255069000},"page":"361-367","source":"Crossref","is-referenced-by-count":3,"title":["Assessment of Proliferative Activity of Glandular Cells in Hyperfunctioning Parathyroid Gland Using Flow Cytometric and Immunohistochemical Methods"],"prefix":"10.1002","volume":"20","author":[{"given":"Pan","family":"Xiao\u2010Lin","sequence":"first","affiliation":[{"name":"Department of Surgery Shishu University School of Medicine Asahi 3\u20101\u20101 Matsumoto 390 Japan<!--<country1>JP<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naohiko","family":"Koide","sequence":"additional","affiliation":[{"name":"Department of Surgery Shishu University School of Medicine Asahi 3\u20101\u20101 Matsumoto 390 Japan<!--<country1>JP<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinya","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Surgery Shishu University School of Medicine Asahi 3\u20101\u20101 Matsumoto 390 Japan<!--<country1>JP<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaru","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Surgery Shishu University School of Medicine Asahi 3\u20101\u20101 Matsumoto 390 Japan<!--<country1>JP<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Sugenoya","sequence":"additional","affiliation":[{"name":"Department of Surgery Shishu University School of Medicine Asahi 3\u20101\u20101 Matsumoto 390 Japan<!--<country1>JP<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Futoshi","family":"Iida","sequence":"additional","affiliation":[{"name":"Department of Surgery Shishu University School of Medicine Asahi 3\u20101\u20101 Matsumoto 390 Japan<!--<country1>JP<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsutomu","family":"Katsuyama","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Shinshu University School of Medicine Asahi 3\u20101\u20101 Matsumoto 390 Japan<!--<country1>JP<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuteru","family":"Usuda","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology Shinshu University School of Medicine Asahi 3\u20101\u20101 Matsumoto 390 Japan<!--<country1>JP<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuji","family":"Nagata","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology Shinshu University School of Medicine Asahi 3\u20101\u20101 Matsumoto 390 Japan<!--<country1>JP<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1996,3]]},"container-title":["World Journal of Surgery"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s002689900059.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s002689900059\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1007\/s002689900059","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T19:47:14Z","timestamp":1705002434000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1007\/s002689900059"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1996,3]]}},"alternative-id":["10.1007\/s002689900059"],"URL":"https:\/\/doi.org\/10.1007\/s002689900059","archive":["Portico"],"relation":{},"ISSN":["0364-2313","1432-2323"],"issn-type":[{"value":"0364-2313","type":"print"},{"value":"1432-2323","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,3]]}}}