{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T02:51:15Z","timestamp":1704077475990},"reference-count":0,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[1996,10,1]],"date-time":"1996-10-01T00:00:00Z","timestamp":844128000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["World j. surg."],"published-print":{"date-parts":[[1996,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The occurrence of graft\u2010versus\u2010host disease (GvHD) following small bowel transplantation (SBTx) can be tuned by the recipient\u2019s initial natural killer (NK) cell activity, which modifies the immunogeneic balance between donor and host immunocompetent cells. This study was aimed to investigate the role of host NK cells on the incidence and severity of GvHD following SBTx. Intraperitoneal administration of 50 \u03bcl ascites fluid of the highly specific anti\u2010NKR\u2010P1\u00a0monoclonal antibody (mAb) 3.2.3\u00a0into F1 recipient animals on three consecutive days prior to SBTx was performed to suppress NK activity in F1 hybrids. In vivo treatment with 3.2.3\u00a0mAb effectively depleted recipient NK activitiy for at least 10\u00a0days in spleens and mesenteric lymph nodes of F1 hosts. In contrast to nontreated F1 recipients, all 3.2.3\u00a0mAb\u2010pretreated F1 animals suffered from severe signs of GvHD, and the mean survival time was decreased significantly from 16.0 \u00b1 0.9\u00a0days to 11.0 \u00b1 0.8\u00a0days (<jats:italic>p<\/jats:italic>&lt; 0.01) in nontreated and NKR\u2010P1\u2010depleted F1 animals, respectively. Other sequelae included earlier onset of GvH manifestations, pronounced damage of primary and secondary lymphatic organs, substantial increase in spleen index, and lower CD4<jats:sup>+<\/jats:sup>\/CD8<jats:sup>+<\/jats:sup>ratios over the course of progressing GvHD. Our results underline the important immunoregulatory role of NK cells as a first defensive line acting on the alloreactivity of donor\u2010derived immunocompetent cells in this model of solid organ transplantation.<\/jats:p>","DOI":"10.1007\/s002689900159","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T05:57:49Z","timestamp":1030255069000},"page":"1041-1051","source":"Crossref","is-referenced-by-count":6,"title":["Correlation between Graft\u2010Versus\u2010Host Induced Immunosuppression and Host Natural Killer Cell Activity in Small Bowel Transplantation"],"prefix":"10.1002","volume":"20","author":[{"given":"Fred","family":"F\u00e4ndrich","sequence":"first","affiliation":[{"name":"Department of General and Thoracic Surgery University of Kiel Arnold\u2010Heller\u2010Strasse 7 24105 Kiel Germany<!--<country1>DE<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f6rg","family":"Schr\u00f6der","sequence":"additional","affiliation":[{"name":"Department of General and Thoracic Surgery University of Kiel Arnold\u2010Heller\u2010Strasse 7 24105 Kiel Germany<!--<country1>DE<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beate","family":"Exner","sequence":"additional","affiliation":[{"name":"Department of General and Thoracic Surgery University of Kiel Arnold\u2010Heller\u2010Strasse 7 24105 Kiel Germany<!--<country1>DE<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Athanasios","family":"Papachrysanthou","sequence":"additional","affiliation":[{"name":"Department of General and Thoracic Surgery University of Kiel Arnold\u2010Heller\u2010Strasse 7 24105 Kiel Germany<!--<country1>DE<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jochen","family":"Peters","sequence":"additional","affiliation":[{"name":"Institute of Pathology University of Kiel Arnold\u2010Heller\u2010Strasse 7 24105 Kiel Germany<!--<country1>DE<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Chambers","sequence":"additional","affiliation":[{"name":"Pittsburgh Cancer Institute University of Pittsburgh Pittsburgh Pennsylvania 15260 U.S.A.<!--<country1>US<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas","family":"Zavazava","sequence":"additional","affiliation":[{"name":"Institute of Immunology University of Kiel Arnold\u2010Heller\u2010Strasse 7 24105 Kiel Germany<!--<country1>DE<\/country1>-->"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1996,10]]},"container-title":["World Journal of Surgery"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s002689900159.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s002689900159\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1007\/s002689900159","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T16:12:26Z","timestamp":1703693546000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1007\/s002689900159"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,10]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1996,10]]}},"alternative-id":["10.1007\/s002689900159"],"URL":"https:\/\/doi.org\/10.1007\/s002689900159","archive":["Portico"],"relation":{},"ISSN":["0364-2313","1432-2323"],"issn-type":[{"value":"0364-2313","type":"print"},{"value":"1432-2323","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,10]]}}}