{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T08:42:23Z","timestamp":1770885743529,"version":"3.50.1"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2008,5,9]],"date-time":"2008-05-09T00:00:00Z","timestamp":1210291200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ann Hematol"],"published-print":{"date-parts":[[2008,8]]},"DOI":"10.1007\/s00277-008-0495-7","type":"journal-article","created":{"date-parts":[[2008,5,8]],"date-time":"2008-05-08T09:54:12Z","timestamp":1210240452000},"page":"639-645","source":"Crossref","is-referenced-by-count":8,"title":["Chromatographic analysis of Hb S for the diagnosis of various sickle cell disorders in Pakistan"],"prefix":"10.1007","volume":"87","author":[{"given":"Nazish Khalid","family":"Hashmi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bushra","family":"Moiz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maliha","family":"Nusrat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mashhooda Rasool","family":"Hashmi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2008,5,9]]},"reference":[{"key":"495_CR1","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1002\/ajmg.10542","volume":"115","author":"BJ Strasser","year":"2002","unstructured":"Strasser BJ (2002) Linus Pauling\u2019s \u201cmolecular diseases\u201d: between history and memory. Am J Med Genet 115:83\u201393","journal-title":"Am J Med Genet"},{"key":"495_CR2","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1038\/180326a0","volume":"180","author":"VM Ingram","year":"1957","unstructured":"Ingram VM (1957) Gene mutations in human haemoglobin: the chemical difference between normal and sickle cell haemoglobin. Nature 180:326\u2013328","journal-title":"Nature"},{"key":"495_CR3","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1056\/NEJMsa013234","volume":"347","author":"S Ahmed","year":"2002","unstructured":"Ahmed S, Saleem M, Modell B, Petrou M (2002) Screening extended families for genetic haemoglobin disorders in Pakistan. N Engl J Med 347:1162\u20131168","journal-title":"N Engl J Med"},{"key":"495_CR4","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1111\/j.1749-6632.1998.tb10482.x","volume":"850","author":"M Angastiniotis","year":"1998","unstructured":"Angastiniotis M, Modell B (1998) Global epidemiology of haemoglobin disorders. Ann N Y Acad Sci 850:251\u2013269","journal-title":"Ann N Y Acad Sci"},{"key":"495_CR5","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1002\/(SICI)1096-8652(200005)64:1<39::AID-AJH7>3.0.CO;2-#","volume":"64","author":"S Daar","year":"2000","unstructured":"Daar S, Hussain HM, Gravell D, Nagel RL, Krishnamoorthy R (2000) Genetic epidemiology of Hb S in Oman: multicentric origin for the betaS gene. Am J Hematol 64:39\u201346","journal-title":"Am J Hematol"},{"key":"495_CR6","first-page":"609","volume":"24","author":"CJ Miller","year":"2003","unstructured":"Miller CJ, Dunn EV, Berg B, Abdouni SF (2003) A hematological survey of preschool children of the United Arab Emirates. Saudi Med J 24:609\u2013613","journal-title":"Saudi Med J"},{"key":"495_CR7","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1159\/000083336","volume":"8","author":"E Baysal","year":"2005","unstructured":"Baysal E (2005) Molecular heterogeneity of beta-thalassemia in the United Arab Emirates. Community Genet 8:35\u201339","journal-title":"Community Genet"},{"key":"495_CR8","first-page":"1168","volume":"21","author":"AG Rajab","year":"2000","unstructured":"Rajab AG, Patton MA, Modell B (2000) Study of hemoglobinopathies in Oman through a national register. Saudi Med J 21:1168\u20131172","journal-title":"Saudi Med J"},{"key":"495_CR9","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1111\/j.1742-1241.1990.tb10073.x","volume":"44","author":"KA Kazmi","year":"1990","unstructured":"Kazmi KA, Rab SM (1990) Sickle cell anaemia in Pakistan. Br J Clin Pract 44:503\u2013505","journal-title":"Br J Clin Pract"},{"key":"495_CR10","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.1093\/clinchem\/46.8.1284","volume":"46","author":"GM Clarke","year":"2000","unstructured":"Clarke GM, Higgins TN (2000) Laboratory investigation of hemoglobinopathies and thalassemias: review and update. Clin Chem 46:1284\u20131290","journal-title":"Clin Chem"},{"key":"495_CR11","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1093\/clinchem\/39.5.820","volume":"39","author":"CN Ou","year":"1993","unstructured":"Ou CN, Rognerud CL (1993) Rapid analysis of haemoglobin variants by cation-exchange HPLC. Clin Chem 39:820\u2013824","journal-title":"Clin Chem"},{"key":"495_CR12","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/S0009-8981(01)00672-6","volume":"313","author":"CN Ou","year":"2001","unstructured":"Ou CN, Rognerud CL (2001) Diagnosis of hemoglobinopathies: electrophoresis vs. HPLC. Clin Chim Acta 313:187\u2013194","journal-title":"HPLC. Clin Chim Acta"},{"key":"495_CR13","first-page":"855","volume":"33","author":"R Ghani","year":"2002","unstructured":"Ghani R, Manji MA, Ahmed N (2002) Haemoglobinopathies among five major ethnic groups in Karachi, Pakistan. Southeast Asian J Trop Med Public Health 33:855\u2013861","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"495_CR14","doi-asserted-by":"crossref","first-page":"1736","DOI":"10.1373\/clinchem.2004.034991","volume":"50","author":"A Joutovsky","year":"2004","unstructured":"Joutovsky A, Hadzi-Nesic J, Nardi MA (2004) HPLC retention time as a diagnostic tool for haemoglobin variants and hemoglobinopathies: a study of 60000 samples in a clinical diagnostic laboratory. Clin Chem 50:1736\u20131747","journal-title":"Clin Chem"},{"key":"495_CR15","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1093\/clinchem\/42.5.704","volume":"42","author":"JW Eastman","year":"1996","unstructured":"Eastman JW, Wong R, Liao CL, Morales DR (1996) Automated HPLC screening of newborns for sickle cell anaemia and other hemoglobinopathies. Clin Chem 42:704\u2013710","journal-title":"Clin Chem"},{"key":"495_CR16","first-page":"191","volume":"30","author":"M Shokrani","year":"2000","unstructured":"Shokrani M, Terrell F, Turner EA, Aguinaga MD (2000) Chromatographic measurements of haemoglobin A2 in blood samples that contain sickle haemoglobin. Ann Clin Lab Sci 30:191\u2013194","journal-title":"Ann Clin Lab Sci"},{"key":"495_CR17","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1093\/clinchem\/43.1.34","volume":"43","author":"J Riou","year":"1997","unstructured":"Riou J, Godart C, Hurtrel D, Mathis M, Bimet C, Bardakdjian-Michau J, Prehu C, Wajcman H, Galacteros F (1997) Cation-exchange HPLC evaluated for presumptive identification of haemoglobin variants. Clin Chem 43:34\u201339","journal-title":"Clin Chem"},{"key":"495_CR18","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1007\/s12098-007-0117-8","volume":"74","author":"RB Colah","year":"2007","unstructured":"Colah RB, Surve R, Sawant P, D\u2019Souza E, Italia K, Phanasgaonkar S, Nadkarni AH, Gorakshakar AC (2007) HPLC studies in hemoglobinopathies. Indian J Pediatr 74:657\u2013662","journal-title":"Indian J Pediatr"},{"key":"495_CR19","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1002\/9780470987056.ch7","volume-title":"Postgraduate hematology","author":"A Lal","year":"2005","unstructured":"Lal A, Vichinsky EP (2005) Sickle cell disease. In: Hoffbrand AV, Catovsky D, Tuddenham EGD (eds) Postgraduate hematology. Blackwell, Oxford, pp 104\u2013118"},{"key":"495_CR20","first-page":"113","volume-title":"Haemoglobinopathy diagnosis","author":"BJ Bain","year":"2001","unstructured":"Bain BJ (2001) Sickle cell haemoglobin and its interactions with other variant haemoglobins and with thalassemias. In: Bain BJ (ed) Haemoglobinopathy diagnosis. 1st edn. Blackwell Science, Oxford, pp 113\u2013153","edition":"1"},{"key":"495_CR21","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1093\/clinchem\/45.7.969","volume":"45","author":"M Campbell","year":"1999","unstructured":"Campbell M, Henthorn JS, Davies SC (1999) Evaluation of cation-exchange HPLC compared with isoelectric focusing for neonatal hemoglobinopathy screening. Clin Chem 45:969\u2013975","journal-title":"Clin Chem"},{"key":"495_CR22","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/s001010050695","volume":"48","author":"T Frietsch","year":"1999","unstructured":"Frietsch T, Segiet W, Schutz P, Haux P, Lorentz A (1999) Perioperative monitoring of haemoglobin fractions in homozygous sickle cell disease. Anaesthesist 48:231\u2013235","journal-title":"Anaesthesist"},{"key":"495_CR23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0950-3536(98)80069-3","volume":"11","author":"J Flint","year":"1998","unstructured":"Flint J, Harding RM, Boyce AJ, Clegg JB (1998) The population genetics of the haemoglobinopathies. Baillieres Clin Haematol 11:1\u201351","journal-title":"Baillieres Clin Haematol"},{"key":"495_CR24","first-page":"883","volume":"64","author":"NP Sharma","year":"1976","unstructured":"Sharma NP, Gupta SC, Atal PR, Mehrotra TN, Agarwal AK, Kapoor KK (1976) Abnormal haemoglobins in the Pakistani Armed Forces personnel. Indian J Med Res 64:883\u2013890","journal-title":"Indian J Med Res"},{"key":"495_CR25","first-page":"1536","volume":"79","author":"RS Balgir","year":"2000","unstructured":"Balgir RS (2000) The burden of haemoglobinopathies in India and the challenges ahead. Current Science 79:1536\u20131547","journal-title":"Current Science"},{"key":"495_CR26","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1002\/ajh.2830480307","volume":"48","author":"M Steinberg","year":"1995","unstructured":"Steinberg M, Hsu H, Nagel R (1995b) Gender and haplotype effects upon hematological manifestation of adult sickle cell anaemia. Am J Hematol 48:175\u2013181","journal-title":"Am J Hematol"},{"key":"495_CR27","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1002\/pd.1523","volume":"26","author":"SM Baig","year":"2006","unstructured":"Baig SM, Azhar A, Hassan H, Baig JM, Aslam M, Ud Din MA, Qureshi JA, Zaman T (2006) Prenatal diagnosis of beta-thalassemia in Southern Punjab, Pakistan. Prenat Diagn 26:903\u2013905","journal-title":"Prenat Diagn"},{"key":"495_CR28","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1136\/jcp.36.9.1012","volume":"36","author":"S Davies","year":"1983","unstructured":"Davies S, Henthorn J, Brozovic M (1983) Iron deficiency in sickle cell anaemia. J Clin Pathol 36:1012\u20131015","journal-title":"J Clin Pathol"},{"key":"495_CR29","first-page":"246","volume":"41","author":"Z Rehman","year":"1991","unstructured":"Rehman Z, Saleem M, Alvi AA, Anwar M, Ahmed PA, Ahmad M (1991) Alpha-thalassaemia: prevalence and pattern in northern Pakistan. J Pak Med Assoc 41:246\u2013247","journal-title":"J Pak Med Assoc"},{"key":"495_CR30","first-page":"118","volume":"42","author":"A Molla","year":"1992","unstructured":"Molla A, Khurshid M, Molla AM (1992) Prevalence of iron deficiency anaemia in children of the urban slums of Karachi. J Pak Med Assoc 42:118\u2013121","journal-title":"J Pak Med Assoc"},{"key":"495_CR31","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1159\/000040853","volume":"100","author":"N Smetanina","year":"1998","unstructured":"Smetanina N, Gu L-H, Huisman T (1998) Comparison of relative quantities of g-messenger RNA and fetal haemoglobin in SS patients with different haplotypes. Acta Hematol 100:4\u20138","journal-title":"Acta Hematol"},{"key":"495_CR32","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1056\/NEJM199709113371107","volume":"337","author":"HF Bunn","year":"1997","unstructured":"Bunn HF (1997) Pathogenesis and treatment of sickle cell disease. N Engl J Med 337:762\u2013769","journal-title":"N Engl J Med"},{"key":"495_CR33","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1373\/clinchem.2005.047985","volume":"51","author":"K Zurbriggen","year":"2005","unstructured":"Zurbriggen K, Schmugge M, Schmid M, Durka S, Kleinert P, Kuster T, Heizmann CW, Troxler H (2005) Analysis of minor hemoglobins by matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry. Clin Chem 51:989\u2013996","journal-title":"Clin Chem"},{"key":"495_CR34","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1136\/jcp.2003.008037","volume":"57","author":"CE Head","year":"2004","unstructured":"Head CE, Conroy M, Jarvis M, Phelan L, Bain BJ (2004) Some observations on the measurement of haemoglobin A2 and S percentages by high performance liquid chromatography in the presence and absence of alpha thalassaemia. J Clin Pathol 57:276\u2013280","journal-title":"J Clin Pathol"},{"key":"495_CR35","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1136\/jcp.2002.007088","volume":"57","author":"JL Camargo","year":"2004","unstructured":"Camargo JL, Gross JL (2004) Conditions associated with very low values of glycohaemoglobin measured by an HPLC method. J Clin Pathol 57:346\u2013349","journal-title":"J Clin Pathol"},{"key":"495_CR36","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1093\/clinchem\/42.1.57","volume":"42","author":"C Papadea","year":"1996","unstructured":"Papadea C, Cate JC (1996) Identification and quantification of hemoglobins A, F, S, and C by automated chromatography. Clin Chem 42:57\u201363","journal-title":"Clin Chem"},{"key":"495_CR37","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1093\/clinchem\/43.9.1667","volume":"43","author":"SI Fisher","year":"1997","unstructured":"Fisher SI, Haga JA, Castleberry SM, Hall RB, Thompson WC (1997) Validation of an automated HPLC method for quantification of haemoglobin S. Clin Chem 43:1667\u20131669","journal-title":"Clin Chem"}],"container-title":["Annals of Hematology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00277-008-0495-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00277-008-0495-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00277-008-0495-7","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T06:13:44Z","timestamp":1684390424000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00277-008-0495-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,5,9]]},"references-count":37,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2008,8]]}},"alternative-id":["495"],"URL":"https:\/\/doi.org\/10.1007\/s00277-008-0495-7","relation":{},"ISSN":["0939-5555","1432-0584"],"issn-type":[{"value":"0939-5555","type":"print"},{"value":"1432-0584","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,5,9]]}}}