{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:45:22Z","timestamp":1792791922665,"version":"4.5.0"},"reference-count":10,"publisher":"Springer Science and Business Media LLC","issue":"9","license":[{"start":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:00:00Z","timestamp":1467331200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Ann Hematol"],"published-print":{"date-parts":[[2016,9]]},"DOI":"10.1007\/s00277-016-2739-2","type":"journal-article","created":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:22:52Z","timestamp":1467332572000},"page":"1559-1560","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Droplet digital PCR assay for quantifying of CALR mutant allelic burden in myeloproliferative neoplasms"],"prefix":"10.1007","volume":"95","author":[{"given":"Luisa","family":"Anelli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Zagaria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicoletta","family":"Coccaro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppina","family":"Tota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Minervini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Casieri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana","family":"Impera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Crescenzio Francesco","family":"Minervini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Brunetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Ricco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Orsini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cosimo","family":"Cumbo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgina","family":"Specchia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Albano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,7,1]]},"reference":[{"key":"2739_CR1","doi-asserted-by":"crossref","first-page":"2391","DOI":"10.1056\/NEJMoa1312542","volume":"369","author":"J Nangalia","year":"2013","unstructured":"Nangalia J, Massie CE, Baxter EJ et al (2013) Somatic CALR mutations in myeloproliferative neoplasms with nonmutated JAK2. N Engl J Med 369:2391\u20132405","journal-title":"N Engl J Med"},{"key":"2739_CR2","doi-asserted-by":"crossref","first-page":"2379","DOI":"10.1056\/NEJMoa1311347","volume":"369","author":"T Klampfl","year":"2013","unstructured":"Klampfl T, Gisslinger H, Harutyunyan AS et al (2013) Somatic mutations of calreticulin in myeloproliferative neoplasms. N Engl J Med 369:2379\u20132390","journal-title":"N Engl J Med"},{"key":"2739_CR3","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.leukres.2014.11.019","volume":"39","author":"AV Jones","year":"2015","unstructured":"Jones AV, Ward D, Lyon M et al (2015) Evaluation of methods to detect CALR mutations in myeloproliferative neoplasms. Leuk Res 39:82\u201387","journal-title":"Leuk Res"},{"key":"2739_CR4","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1007\/s00277-014-2232-8","volume":"94","author":"J Chi","year":"2015","unstructured":"Chi J, Manoloukos M, Pierides C et al (2015) Calreticulin mutations in myeloproliferative neoplasms and new methodology for their detection and monitoring. Ann Hematol 94:399\u2013408","journal-title":"Ann Hematol"},{"key":"2739_CR5","doi-asserted-by":"crossref","first-page":"13269","DOI":"10.18632\/oncotarget.3773","volume":"6","author":"F Albano","year":"2015","unstructured":"Albano F, Zagaria A, Anelli L et al (2015) Absolute quantification of the pretreatment PML-RARA transcript defines the relapse risk in acute promyelocytic leukemia. Oncotarget 6:13269\u201313277","journal-title":"Oncotarget"},{"key":"2739_CR6","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1016\/j.jmoldx.2015.05.007","volume":"17","author":"D Drandi","year":"2015","unstructured":"Drandi D, Kubiczkova-Besse L, Ferrero S et al (2015) Minimal residual disease detection by droplet digital PCR in multiple myeloma, mantle cell lymphoma, and follicular lymphoma: a comparison with real-time PCR. J Mol Diagn 17:652\u2013660","journal-title":"J Mol Diagn"},{"key":"2739_CR7","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1007\/s00428-015-1802-z","volume":"467","author":"A Zagaria","year":"2015","unstructured":"Zagaria A, Anelli L, Coccaro N et al (2015) BCR-ABL1 e6a2 transcript in chronic myeloid leukemia: biological features and molecular monitoring by droplet digital PCR. Virchows Arch 467:357\u2013363","journal-title":"Virchows Arch"},{"key":"2739_CR8","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.jmoldx.2015.07.007","volume":"18","author":"O Mansier","year":"2016","unstructured":"Mansier O, Migeon M, Saint-L\u00e9zer A et al (2016) Quantification of the mutant CALR allelic burden by digital PCR: application to minimal residual disease evaluation after bone marrow transplantation. J Mol Diagn 18:68\u201374","journal-title":"J Mol Diagn"},{"issue":"6","key":"2739_CR9","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1038\/bmt.2016.14","volume":"51","author":"A Badbaran","year":"2016","unstructured":"Badbaran A, Fehse B, Christopeit M et al (2016) Digital-PCR assay for screening and quantitative monitoring of calreticulin (CALR) type-2 positive patients with myelofibrosis following allogeneic stem cell transplantation. Bone Marrow Transplant 51(6):872\u2013873","journal-title":"Bone Marrow Transplant"},{"key":"2739_CR10","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1056\/NEJMc1401255","volume":"371","author":"B Cassinat","year":"2014","unstructured":"Cassinat B, Verger E, Kiladjian JJ (2014) Interferon alfa therapy in CALR-mutated essential thrombocythemia. N Engl J Med 371:188\u2013189","journal-title":"N Engl J Med"}],"container-title":["Annals of Hematology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00277-016-2739-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00277-016-2739-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00277-016-2739-2","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,6,24]],"date-time":"2017-06-24T17:33:58Z","timestamp":1498325638000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00277-016-2739-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7,1]]},"references-count":10,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2016,9]]}},"alternative-id":["2739"],"URL":"https:\/\/doi.org\/10.1007\/s00277-016-2739-2","relation":{},"ISSN":["0939-5555","1432-0584"],"issn-type":[{"value":"0939-5555","type":"print"},{"value":"1432-0584","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7,1]]}}}