{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T05:06:29Z","timestamp":1790485589626,"version":"4.1.0"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,10,26]],"date-time":"2020-10-26T00:00:00Z","timestamp":1603670400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2020,10,26]],"date-time":"2020-10-26T00:00:00Z","timestamp":1603670400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB\/TRR57, SFB\/TRR219, BO3755\/3-1, BO3755\/6-1"],"award-info":[{"award-number":["SFB\/TRR57, SFB\/TRR219, BO3755\/3-1, BO3755\/6-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["KO2155\/7-1"],"award-info":[{"award-number":["KO2155\/7-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SCHN1188\/6-1"],"award-info":[{"award-number":["SCHN1188\/6-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["STOP-FSGS-01GM1901A"],"award-info":[{"award-number":["STOP-FSGS-01GM1901A"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009379","name":"Interdisziplin\u00e4res Zentrum f\u00fcr Klinische Forschung, Universit\u00e4tsklinikum W\u00fcrzburg","doi-asserted-by":"crossref","award":["O3-2"],"award-info":[{"award-number":["O3-2"]}],"id":[{"id":"10.13039\/501100009379","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Ann Hematol"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Bone marrow (BM) fibrosis in myeloproliferative neoplasms (MPNs) is associated with a poor prognosis. The development of myelofibrosis and differentiation of mesenchymal stromal cells to profibrotic myofibroblasts depends on macrophages. Here, we compared macrophage frequencies in BM biopsies of MPN patients and controls (patients with non-neoplastic processes), including primary myelofibrosis (PMF,\n                    <jats:italic>n<\/jats:italic>\n                    = 18), essential thrombocythemia (ET,\n                    <jats:italic>n<\/jats:italic>\n                    = 14), polycythemia vera (PV,\n                    <jats:italic>n<\/jats:italic>\n                    = 12), and Philadelphia chromosome\u2013positive chronic myeloid leukemia (CML,\n                    <jats:italic>n<\/jats:italic>\n                    = 9). In PMF, CD68-positive macrophages were greatly increased compared to CML (\n                    <jats:italic>p<\/jats:italic>\n                    = 0.017) and control BM (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001). Similar findings were observed by CD163 staining (PMF vs. CML:\n                    <jats:italic>p<\/jats:italic>\n                    = 0.017; PMF vs. control:\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001). Moreover, CD68-positive macrophages were increased in PV compared with ET (\n                    <jats:italic>p<\/jats:italic>\n                    = 0.009) and reactive cases (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001). PMF had higher frequencies of macrophages than PV (CD68:\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001; CD163:\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001) and ET (CD68:\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001; CD163:\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001). CD163 and CD68 were often co-expressed in macrophages with stellate morphology in Philadelphia chromosome\u2013negative MPN, resulting in a sponge-like reticular network that may be a key regulator of unbalanced hematopoiesis in the BM space and may explain differences in cellularity and clinical course.\n                  <\/jats:p>","DOI":"10.1007\/s00277-020-04304-y","type":"journal-article","created":{"date-parts":[[2020,10,26]],"date-time":"2020-10-26T04:03:05Z","timestamp":1603684985000},"page":"97-104","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Macrophage frequency in the bone marrow correlates with morphologic subtype of myeloproliferative neoplasm"],"prefix":"10.1007","volume":"100","author":[{"given":"David C. A.","family":"Molitor","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9921-4284","authenticated-orcid":false,"given":"Peter","family":"Boor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Buness","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebekka K.","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lino L.","family":"Teichmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth-Miriam","family":"K\u00f6rber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0309-595X","authenticated-orcid":false,"given":"Gabor L.","family":"Horvath","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1011-8171","authenticated-orcid":false,"given":"Steffen","family":"Koschmieder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2609-3095","authenticated-orcid":false,"given":"Ines","family":"G\u00fctgemann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,10,26]]},"reference":[{"key":"4304_CR1","doi-asserted-by":"publisher","first-page":"e1345402","DOI":"10.1080\/2162402X.2017.1345402","volume":"6","author":"M Romano","year":"2017","unstructured":"Romano M, Sollazzo D, Trabanelli S, Barone M, Polverelli N, Perricone M, Forte D, Luatti S, Cavo M, Vianelli N, Jandus C, Palandri F, Catani L (2017) Mutations in JAK2 and Calreticulin genes are associated with specific alterations of the immune system in myelofibrosis. Oncoimmunology 6:e1345402. https:\/\/doi.org\/10.1080\/2162402X.2017.1345402","journal-title":"Oncoimmunology"},{"key":"4304_CR2","doi-asserted-by":"publisher","first-page":"1062","DOI":"10.1182\/blood-2014-05-578435","volume":"124","author":"E Rumi","year":"2014","unstructured":"Rumi E, Pietra D, Pascutto C, Guglielmelli P, Mart\u00ednez-Trillos A, Casetti I, Colomer D, Pieri L, Pratcorona M, Rotunno G, Sant'Antonio E, Bellini M, Cavalloni C, Mannarelli C, Milanesi C, Boveri E, Ferretti V, Astori C, Rosti V, Cervantes F, Barosi G, Vannucchi AM, Cazzola M, Associazione Italiana per la Ricerca sul Cancro Gruppo Italiano Malattie Mieloproliferative Investigators (2014) Clinical effect of driver mutations of JAK2, CALR, or MPL in primary myelofibrosis. 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The procedures used in this study adhere to the tenets of the Helsinki Declaration of 1975 and as revised in 2008.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"Statement of informed consent from individual patients was not applicable for this type of retrospective analysis according to the regulation of the ethical board.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"It was obtained prior to submission from all co-authors.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}}]}}