{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:30:52Z","timestamp":1792621852616,"version":"4.5.0"},"reference-count":17,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2020,10,23]],"date-time":"2020-10-23T00:00:00Z","timestamp":1603411200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,10,23]],"date-time":"2020-10-23T00:00:00Z","timestamp":1603411200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/100006396","name":"Alexion Pharmaceuticals","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006396","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Ann Hematol"],"published-print":{"date-parts":[[2021,8]]},"DOI":"10.1007\/s00277-020-04314-w","type":"journal-article","created":{"date-parts":[[2020,10,23]],"date-time":"2020-10-23T03:02:47Z","timestamp":1603422167000},"page":"1975-1982","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The clinical significance of PNH-phenotype cells accounting for < 0.01% of total granulocytes detected by the Clinical and Laboratory Standards Institute methods in patients with bone marrow failure"],"prefix":"10.1007","volume":"100","author":[{"given":"Kohei","family":"Hosokawa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Ishiyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiyuki","family":"Ikemoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiharu","family":"Sugimori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideyoshi","family":"Noji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsutomu","family":"Shichishima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoshi","family":"Obara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeru","family":"Chiba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruhiko","family":"Ninomiya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai Anh Thi","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukari","family":"Shirasugi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiko","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyoshi","family":"Ando","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasutaka","family":"Ueda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Yonemura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuya","family":"Kawaguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-ichi","family":"Nishimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuzuru","family":"Kanakura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Nakao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,10,23]]},"reference":[{"issue":"6","key":"4314_CR1","doi-asserted-by":"publisher","first-page":"401","DOI":"10.7326\/0003-4819-131-6-199909210%-00002","volume":"131","author":"DE Dunn","year":"1999","unstructured":"Dunn DE, Tanawattanacharoen P, Boccuni P, Nagakura S, Green SW, Kirby MR, Kumar MS, Rosenfeld S, Young NS (1999) Paroxysmal nocturnal hemoglobinuria cells in patients with bone marrow failure syndromes. Ann Intern Med 131(6):401\u2013408. https:\/\/doi.org\/10.7326\/0003-4819-131-6-199909210%-00002","journal-title":"Ann Intern Med"},{"issue":"4","key":"4314_CR2","doi-asserted-by":"publisher","first-page":"1015","DOI":"10.1046\/j.1365-2141.2001.03191.x","volume":"115","author":"JP Maciejewski","year":"2001","unstructured":"Maciejewski JP, Rivera C, Kook H, Dunn D, Young NS (2001) Relationship between bone marrow failure syndromes and the presence of glycophosphatidyl inositol-anchored protein-deficient clones. Br J Haematol 115(4):1015\u20131022. https:\/\/doi.org\/10.1046\/j.1365-2141.2001.03191.x","journal-title":"Br J Haematol"},{"issue":"1","key":"4314_CR3","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1111\/j.1365-2141.2009.07822.x","volume":"147","author":"C Sugimori","year":"2009","unstructured":"Sugimori C, Mochizuki K, Qi Z, Sugimori N, Ishiyama K, Kondo Y, Yamazaki H, Takami A, Okumura H, Nakao S (2009) Origin and fate of blood cells deficient in glycosylphosphatidylinositol-anchored protein among patients with bone marrow failure. Br J Haematol 147(1):102\u2013112. https:\/\/doi.org\/10.1111\/j.1365-2141.2009.07822.x","journal-title":"Br J Haematol"},{"issue":"3","key":"4314_CR4","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1111\/ejh.12484","volume":"95","author":"K Hosokawa","year":"2015","unstructured":"Hosokawa K, Sugimori N, Katagiri T, Sasaki Y, Saito C, Seiki Y, Mochizuki K, Yamazaki H, Takami A, Nakao S (2015) Increased glycosylphosphatidylinositol-anchored protein-deficient granulocytes define a benign subset of bone marrow failures in patients with trisomy 8. Eur J Haematol 95(3):230\u2013238. https:\/\/doi.org\/10.1111\/ejh.12484","journal-title":"Eur J Haematol"},{"issue":"4","key":"4314_CR5","doi-asserted-by":"publisher","first-page":"546","DOI":"10.1111\/bjh.12661","volume":"164","author":"A Kulagin","year":"2014","unstructured":"Kulagin A, Lisukov I, Ivanova M, Golubovskaya I, Kruchkova I, Bondarenko S, Vavilov V, Stancheva N, Babenko E, Sipol A, Pronkina N, Kozlov V, Afanasyev B (2014) Prognostic value of paroxysmal nocturnal haemoglobinuria clone presence in aplastic anaemia patients treated with combined immunosuppression: results of two-centre prospective study. Br J Haematol 164(4):546\u2013554. https:\/\/doi.org\/10.1111\/bjh.12661","journal-title":"Br J Haematol"},{"issue":"12","key":"4314_CR6","doi-asserted-by":"publisher","first-page":"1546","DOI":"10.3324\/haematol.2015.132530","volume":"100","author":"A Narita","year":"2015","unstructured":"Narita A, Muramatsu H, Sekiya Y, Okuno Y, Sakaguchi H, Nishio N, Yoshida N, Wang X, Xu Y, Kawashima N, Doisaki S, Hama A, Takahashi Y, Kudo K, Moritake H, Kobayashi M, Kobayashi R, Ito E, Yabe H, Ohga S, Ohara A, Kojima S, Japan Childhood Aplastic Anemia Study G (2015) Paroxysmal nocturnal hemoglobinuria and telomere length predicts response to immunosuppressive therapy in pediatric aplastic anemia. Haematologica 100(12):1546\u20131552. https:\/\/doi.org\/10.3324\/haematol.2015.132530","journal-title":"Haematologica"},{"issue":"4","key":"4314_CR7","doi-asserted-by":"publisher","first-page":"1308","DOI":"10.1182\/blood-2005%-06-2485","volume":"107","author":"C Sugimori","year":"2006","unstructured":"Sugimori C, Chuhjo T, Feng X, Yamazaki H, Takami A, Teramura M, Mizoguchi H, Omine M, Nakao S (2006) Minor population of CD55-CD59- blood cells predicts response to immunosuppressive therapy and prognosis in patients with aplastic anemia. Blood 107(4):1308\u20131314. https:\/\/doi.org\/10.1182\/blood-2005%-06-2485","journal-title":"Blood"},{"issue":"12","key":"4314_CR8","doi-asserted-by":"publisher","first-page":"1845","DOI":"10.3324\/haematol.2011.061127","volume":"97","author":"K Hosokawa","year":"2012","unstructured":"Hosokawa K, Katagiri T, Sugimori N, Ishiyama K, Sasaki Y, Seiki Y, Sato-Otsubo A, Sanada M, Ogawa S, Nakao S (2012) Favorable outcome of patients who have 13q deletion: a suggestion for revision of the WHO 'MDS-U' designation. Haematologica 97(12):1845\u20131849. https:\/\/doi.org\/10.3324\/haematol.2011.061127","journal-title":"Haematologica"},{"issue":"4","key":"4314_CR9","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1002\/cyto.b.20525","volume":"78","author":"MJ Borowitz","year":"2010","unstructured":"Borowitz MJ, Craig FE, Digiuseppe JA, Illingworth AJ, Rosse W, Sutherland DR, Wittwer CT, Richards SJ, Clinical Cytometry S (2010) Guidelines for the diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria and related disorders by flow cytometry. Cytometry B Clin Cytom 78(4):211\u2013230. https:\/\/doi.org\/10.1002\/cyto.b.20525","journal-title":"Cytometry B Clin Cytom"},{"issue":"4","key":"4314_CR10","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1002\/cyto.b.21075","volume":"84","author":"I Marinov","year":"2013","unstructured":"Marinov I, Kohoutova M, Tkacova V, Lysak D, Holubova M, Stehlikova O, Zeleznikova T, Zontar D, Illingworth A (2013) Intra- and interlaboratory variability of paroxysmal nocturnal hemoglobinuria testing by flow cytometry following the 2012 Practical Guidelines for high-sensitivity paroxysmal nocturnal hemoglobinuria testing. Cytometry B Clin Cytom 84(4):229\u2013236. https:\/\/doi.org\/10.1002\/cyto.b.21075","journal-title":"Cytometry B Clin Cytom"},{"issue":"4","key":"4314_CR11","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1002\/cyto.b.21023","volume":"82","author":"DR Sutherland","year":"2012","unstructured":"Sutherland DR, Keeney M, Illingworth A (2012) Practical guidelines for the high-sensitivity detection and monitoring of paroxysmal nocturnal hemoglobinuria clones by flow cytometry. Cytometry B Clin Cytom 82(4):195\u2013208. https:\/\/doi.org\/10.1002\/cyto.b.21023","journal-title":"Cytometry B Clin Cytom"},{"issue":"3","key":"4314_CR12","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1002\/cyto.b.21139","volume":"86","author":"A Raza","year":"2014","unstructured":"Raza A, Ravandi F, Rastogi A, Bubis J, Lim SH, Weitz I, Castro-Malaspina H, Galili N, Jawde RA, Illingworth A (2014) A prospective multicenter study of paroxysmal nocturnal hemoglobinuria cells in patients with bone marrow failure. Cytometry B Clin Cytom 86(3):175\u2013182. https:\/\/doi.org\/10.1002\/cyto.b.21139","journal-title":"Cytometry B Clin Cytom"},{"issue":"5","key":"4314_CR13","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1111\/bjh.12656","volume":"164","author":"PR Tutelman","year":"2014","unstructured":"Tutelman PR, Aubert G, Milner RA, Dalal BI, Schultz KR, Deyell RJ (2014) Paroxysmal nocturnal haemoglobinuria phenotype cells and leucocyte subset telomere length in childhood acquired aplastic anaemia. Br J Haematol 164(5):717\u2013721. https:\/\/doi.org\/10.1111\/bjh.12656","journal-title":"Br J Haematol"},{"issue":"12","key":"4314_CR14","doi-asserted-by":"publisher","first-page":"2289","DOI":"10.1007\/s00277%-018-3443-1","volume":"97","author":"K Hosokawa","year":"2018","unstructured":"Hosokawa K, Sugimori C, Ishiyama K, Takamatsu H, Noji H, Shichishima T, Obara N, Chiba S, Ninomiya H, Shirasugi Y, Nakamura Y, Ando K, Ueda Y, Yonemura Y, Kawaguchi T, Nishimura JI, Kanakura Y, Nakao S (2018) Establishment of a flow cytometry assay for detecting paroxysmal nocturnal hemoglobinuria-type cells specific to patients with bone marrow failure. Ann Hematol 97(12):2289\u20132297. https:\/\/doi.org\/10.1007\/s00277%-018-3443-1","journal-title":"Ann Hematol"},{"issue":"22","key":"4314_CR15","doi-asserted-by":"publisher","first-page":"3896","DOI":"10.1182\/blood.V128.22.3896.3896","volume":"128","author":"Y Ueda","year":"2016","unstructured":"Ueda Y, Nishimura J-I, Sugimori C, Hosokawa K, Yonemura Y, Obara N, Noji H, Nakamura Y, Shirasugi Y, Ando K, Shichishima T, Ninomiya H, Chiba S, Kawaguchi T, Kanakura Y, Nakao S (2016) The first follow-up data analysis of patients with acquired bone marrow failure harboring a small population of PNH-type cells in the Japanese, Multicenter, Prospective Study Optima. Blood 128(22):3896\u20133896. https:\/\/doi.org\/10.1182\/blood.V128.22.3896.3896","journal-title":"Blood"},{"key":"4314_CR16","unstructured":"Sakamoto J, Noma Y (2017) Performance evaluation of the high sensitive PNH clone measurement in conformity with ICCS guidelines. Cytometry Research 27 (1):1-6. doi:10.18947\/cytometryresearch.27.1_1"},{"issue":"Supplement_1","key":"4314_CR17","doi-asserted-by":"publisher","first-page":"1218","DOI":"10.1182\/blood-2019-123415","volume":"134","author":"M Le Garff-Tavernier","year":"2019","unstructured":"Le Garff-Tavernier M, Pruvot Debliquis A, Boyer T, Vial J-P, Harrivel V, Lhoumeau A-C, Jacob M-C, Socie G, Peffault de Latour R, Drenou B, Wagner Ballon O (2019) Persistence of PNH clones over time: insights from the mid-term analysis of the French Nation-Wide Multicenter Prospective Observational Study. Blood 134(Supplement_1):1218\u20131218. https:\/\/doi.org\/10.1182\/blood-2019-123415","journal-title":"Blood"}],"container-title":["Annals of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00277-020-04314-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00277-020-04314-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00277-020-04314-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,22]],"date-time":"2021-10-22T20:08:26Z","timestamp":1634933306000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00277-020-04314-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,23]]},"references-count":17,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["4314"],"URL":"https:\/\/doi.org\/10.1007\/s00277-020-04314-w","relation":{},"ISSN":["0939-5555","1432-0584"],"issn-type":[{"value":"0939-5555","type":"print"},{"value":"1432-0584","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,23]]},"assertion":[{"value":"4 April 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 October 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 October 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"Dr. Ninomiya, Dr. Shichishima, Dr. Yonemura, Dr. Kawaguchi, and Dr. Nakao report personal fees from Alexion Pharma G.K. outside the submitted work; Dr. Ninomiya, Dr. Chiba, Dr. Ueda, Dr. Yonemura, and Dr. Nakao report grants from Alexion Pharma G.K. outside the submitted work; Dr. Kanakura and Dr. Nishimura report grants and personal fees from Alexion Pharma G.K. during the conduct of the study; Dr. Shirasugi reports honoraria from Novartis; Dr. Hosokawa, Dr. Ishiyama, Dr. Sugimori, Dr. Noji, Dr. Obara, Dr. Thi Nguyen, Dr. Nakamura, and Dr. Ando have nothing to declare.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}