{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T07:49:38Z","timestamp":1793346578954,"version":"4.5.0"},"reference-count":10,"publisher":"Springer Science and Business Media LLC","issue":"10","license":[{"start":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T00:00:00Z","timestamp":1752537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T00:00:00Z","timestamp":1752537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Ann Hematol"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>To evaluate performance of AI-assisted blast detection via whole blood film scanning in cases of severe leukopenia. From August to October 2023, we collected 157 patient specimens with white blood cell (WBC) counts of \u22642.0 \u00d7 10\u2079\/L. We then evaluated and compared the blast detection capabilities of an artificial intelligence-based system (Cygnus instrument) against the CellaVision DI-60 for WBC analysis. Additionally, this study aimed to assess the comparative performance of these two automated blood cell morphology analyzers based on their underlying operational principles. Following manual microscopy confirmation, 17 cases showing\u2009\u2265\u20091 blast cell in peripheral blood smear were identified among 157 specimens with WBC\u2009\u2264\u20092.0\u2009\u00d7\u200910\u2079\/L. The Cygnus whole-slide scanning mode detected all 17 cases (sensitivity: 100%), whereas the Cygnus 200-cell mode detected only 9 cases (9\/17), and the CellaVision DI-60 200-cell mode detected 8 cases (8\/17).\u00a0In leukopenic patients (WBC \u22642.0\u00d710\u2079\/L), utilizing Cygnus' whole-slide scanning capability substantially improves blast identification rates, potentially enabling earlier detection of hematologic abnormalities.<\/jats:p>","DOI":"10.1007\/s00277-025-06473-0","type":"journal-article","created":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T06:51:21Z","timestamp":1752562281000},"page":"5415-5422","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Advantages of AI-based whole blood film scanning for blast detection in markedly leucopenic blood films"],"prefix":"10.1007","volume":"104","author":[{"given":"Geng","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhejun","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoju","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi Qi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo Shen","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongrong","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binyao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,15]]},"reference":[{"key":"6473_CR1","doi-asserted-by":"publisher","first-page":"101144","DOI":"10.1016\/j.blre.2023.101144","volume":"64","author":"BE Fan","year":"2024","unstructured":"Fan BE, Yong BSJ, Li R, Wang SSY, Aw MYN, Chia MF, Chen DTY, Neo YS, Occhipinti B, Ling RR, Ramanathan K, Ong YX, Lim KGE, Wong WYK, Lim SP, Latiff STBA, Shanmugam H, Wong MS, Ponnudurai K, Winkler S (2024) From microscope to micropixels: A rapid review of artificial intelligence for the peripheral blood film. Blood Rev 64:101144. https:\/\/doi.org\/10.1016\/j.blre.2023.101144","journal-title":"Blood Rev"},{"issue":"3","key":"6473_CR2","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1111\/ijlh.12327","volume":"37","author":"L Palmer","year":"2015","unstructured":"Palmer L, Briggs C, McFadden S, Zini G, Burthem J, Rozenberg G, Proytcheva M, Machin SJ (2015) ICSH recommendations for the standardization of nomenclature and grading of peripheral blood cell morphological features. Int J Lab Hematol 37(3):287\u2013303. https:\/\/doi.org\/10.1111\/ijlh.12327","journal-title":"Int J Lab Hematol"},{"issue":"23","key":"6473_CR3","doi-asserted-by":"publisher","first-page":"1744","DOI":"10.1055\/s-0043-113123","volume":"142","author":"D Christen","year":"2017","unstructured":"Christen D, Br\u00fcmmendorf TH, Panse J (2017) Leukopenie\u2013 ein diagnostischer Leitfaden f\u00fcr die praxis Leukopenia - A diagnostic guideline for the clinical routine. Dtsch Med Wochenschr 142(23):1744\u20131749 German. https:\/\/doi.org\/10.1055\/s-0043-113123","journal-title":"Dtsch Med Wochenschr"},{"issue":"1","key":"6473_CR4","doi-asserted-by":"publisher","first-page":"9","DOI":"10.4103\/2153-3539.129442","volume":"5","author":"EA Mohammed","year":"2014","unstructured":"Mohammed EA, Mohamed MM, Far BH, Naugler C (2014) Peripheral blood smear image analysis: A comprehensive review. J Pathol Inf 5(1):9. https:\/\/doi.org\/10.4103\/2153-3539.129442","journal-title":"J Pathol Inf"},{"issue":"1","key":"6473_CR5","doi-asserted-by":"publisher","first-page":"44","DOI":"10.3343\/alm.2021.41.1.44","volume":"41","author":"SJ Park","year":"2021","unstructured":"Park SJ, Yoon J, Kwon JA, Yoon SY (2021) Evaluation of the cellavision advanced RBC application for detecting red blood cell morphological abnormalities. 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Cancer Commun (Lond) 40(4):154\u2013166. https:\/\/doi.org\/10.1002\/cac2.12012","journal-title":"Cancer Commun (Lond)"},{"issue":"4","key":"6473_CR8","doi-asserted-by":"publisher","first-page":"398","DOI":"10.3343\/alm.2022.42.4.398","volume":"42","author":"M Nam","year":"2022","unstructured":"Nam M, Yoon S, Hur M, Lee GH, Kim H, Park M, Kim HN (2022) Digital morphology analyzer sysmex DI-60 vs. Manual counting for white blood cell differentials in leukopenic samples: A comparative assessment of risk and turnaround time. Ann Lab Med 42(4):398\u2013405","journal-title":"Ann Lab Med"},{"issue":"12","key":"6473_CR9","doi-asserted-by":"publisher","first-page":"2173","DOI":"10.3390\/diagnostics11122173","volume":"11","author":"S Yoon","year":"2021","unstructured":"Yoon S, Hur M, Lee GH, Nam M, Kim H (2021) How reproducible is the data from sysmex DI-60 in leukopenic samples? 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Sci Rep 14(1):14344. https:\/\/doi.org\/10.1038\/s41598-024-65427-0","journal-title":"Sci Rep"}],"container-title":["Annals of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00277-025-06473-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00277-025-06473-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00277-025-06473-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T08:18:51Z","timestamp":1763194731000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00277-025-06473-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,15]]},"references-count":10,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,10]]}},"alternative-id":["6473"],"URL":"https:\/\/doi.org\/10.1007\/s00277-025-06473-0","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-5813808\/v1","asserted-by":"object"}]},"ISSN":["1432-0584"],"issn-type":[{"value":"1432-0584","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,15]]},"assertion":[{"value":"12 January 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 June 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 July 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The study was reviewed and approved by the Ethics Review Committee of Peking Union Medical College Hospital, Chinese Academy of Medical Sciences.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"<!--Emphasis Type='Bold' removed-->Ethics review statement"}},{"value":"A total of 157 EDTA-K2 anticoagulated whole blood samples were collected from outpatients and inpatients at Peking Union Medical College Hospital between August and October 2023. This study was approved by the Institutional Review Board (IRB) of Peking Union Medical College Hospital (Approval No. S-T594) and conducted in compliance with the Declaration of Helsinki (1964). All samples were stored at room temperature (18\u201322 \u00b0C) and processed within 2\u00a0h of collection.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"<!--Emphasis Type='Bold' removed-->Human and animal protection"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"All authors agree to publication.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Author\u2019s statement"}}]}}