{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T22:24:52Z","timestamp":1787610292228,"version":"build-2736575974"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T00:00:00Z","timestamp":1779062400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"vor","delay-in-days":52,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100014718","name":"Innovative Research Group Project of the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82270142"],"award-info":[{"award-number":["82270142"]}],"id":[{"id":"10.13039\/100014718","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Tianjin Municipal Natural Science Foundation","award":["24ZGSSSS00050"],"award-info":[{"award-number":["24ZGSSSS00050"]}]},{"name":"the Tianjin Science and Technology Planning Project","award":["24ZXGZSY00090"],"award-info":[{"award-number":["24ZXGZSY00090"]}]},{"name":"Tianjin Municipal Health Commission Project","award":["TJWJ2023XK003"],"award-info":[{"award-number":["TJWJ2023XK003"]}]},{"name":"Tianjin Key Medical Discipline Construction Project","award":["TJYXZDXK-3-001A"],"award-info":[{"award-number":["TJYXZDXK-3-001A"]}]},{"name":"Tianjin Municipal Health Commission Integrated Traditional Chinese and Western Medicine Research Project","award":["2023036"],"award-info":[{"award-number":["2023036"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Ann Hematol"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Aplasticanemia (AA) is an autoimmune-mediated bone marrow failure syndrome characterized by excessive T-cell activation and destruction of hematopoietic stem and progenitor cells. Eltrombopag (ELT), a thrombopoietin receptor agonist, has been shown to promote hematopoiesis and exert immunomodulatory effects. However, its role in regulating T cells in AA remains unclear. This study aimed to investigate whether ELT modulates the T-cell functional phenotype via the oxidative metabolism pathway. Using single-cell RNA sequencing of AA mouse models under different treatment regimens, we identified differentially expressed genes in T-cell subsets associated with oxidative stress. ELT treatment effectively reduced reactive oxygen species (ROS) levels in both CD4\u207a and CD8\u207a T cells, reshaped T-cell subset composition, and attenuated cytotoxic activity. Mechanistically, Western blot analysis confirmed that ELT significantly upregulated ENPP1 and ENTPD5, which are involved in oxidative metabolism. Collectively, these findings reveal a previously unrecognized mechanism through which ELT restores T-cell homeostasis by fine-tuning oxidative metabolism, highlighting its therapeutic potential beyond hematopoietic stimulation in AA.<\/jats:p>","DOI":"10.1007\/s00277-026-07060-7","type":"journal-article","created":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T00:14:51Z","timestamp":1779063291000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Eltrombopag restores T-cell homeostasis in aplastic anemia by regulating oxidative metabolism and reactive oxygen species levels"],"prefix":"10.1007","volume":"105","author":[{"given":"Ting","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiulin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boyi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nianbin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,18]]},"reference":[{"key":"7060_CR1","doi-asserted-by":"crossref","unstructured":"Young NS, Aplastic Anemia (2018) N Engl J Med 379(17):1643\u20131656 doi:10\/gjxrsn","DOI":"10.1056\/NEJMra1413485"},{"key":"7060_CR2","doi-asserted-by":"crossref","unstructured":"Giudice V, Selleri C (2022) Aplastic anemia: Pathophysiology. 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Written informed consent was obtained from all AA patients and healthy donors in accordance with the Declaration of Helsinki.","order":3,"name":"Ethics","label":"Disclosure","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":4,"name":"Ethics","label":"Clinical trial number","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"323"}}