{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T03:14:06Z","timestamp":1793157246455,"version":"4.5.0"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2013,8,10]],"date-time":"2013-08-10T00:00:00Z","timestamp":1376092800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Curr Microbiol"],"published-print":{"date-parts":[[2014,1]]},"DOI":"10.1007\/s00284-013-0436-0","type":"journal-article","created":{"date-parts":[[2013,8,9]],"date-time":"2013-08-09T11:37:51Z","timestamp":1376048271000},"page":"21-29","source":"Crossref","is-referenced-by-count":26,"title":["Identification of Intracellular Bacteria in Adenoid and Tonsil Tissue Specimens: The Efficiency of Culture Versus Fluorescent In Situ Hybridization (FISH)"],"prefix":"10.1007","volume":"68","author":[{"given":"M.","family":"St\u0119pi\u0144ska","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Olszewska-Sosi\u0144ska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Lau-Dworak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Zielnik-Jurkiewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. A.","family":"Trafny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,8,10]]},"reference":[{"key":"436_CR1","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1128\/jb.172.2.762-770.1990","volume":"172","author":"R Amann","year":"1990","unstructured":"Amann R, Krumholz L, Stahl DA (1990) Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J Bacteriol 172:762\u2013770","journal-title":"J Bacteriol"},{"key":"436_CR2","doi-asserted-by":"crossref","first-page":"150","DOI":"10.3109\/08820139.2010.517589","volume":"40","author":"A Ben-Yaakov","year":"2011","unstructured":"Ben-Yaakov A, Maly B, Abu-Ita R, Elidan J, Gross M (2011) Identification and immunolocalization of the innate immune receptor CD14 in hypertrophic adenoids and tonsils. Immunol Invest 40:150\u2013159","journal-title":"Immunol Invest"},{"key":"436_CR3","doi-asserted-by":"crossref","first-page":"e34083","DOI":"10.1371\/journal.pone.0034083","volume":"7","author":"MJ Binks","year":"2012","unstructured":"Binks MJ, Temple B, Kirkham LA et al (2012) Molecular surveillance of true nontypeable Haemophilus influenzae: an evaluation of PCR screening assays. PLoS ONE 7:e34083. doi: 10.1371\/journal.pone.0034083","journal-title":"PLoS ONE"},{"key":"436_CR4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fcimb.2011.00001","volume":"1","author":"CF Clementi","year":"2011","unstructured":"Clementi CF, Murphy TF (2011) Non-typeable Haemophilus influenzae invasion and persistence in the human respiratory tract. Front Cell Infect Microbiol 1:1. doi: 10.3389\/fcimb.2011.00001","journal-title":"Front Cell Infect Microbiol"},{"key":"436_CR5","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1111\/j.1574-6968.2001.tb10820.x","volume":"203","author":"JE Craig","year":"2001","unstructured":"Craig JE, Cliffe A, Garnett K, High NJ (2001) Survival of nontypeable Haemophilus influenzae in macrophages. FEMS Microbiol Lett 203:55\u201361. doi: 10.1016\/S0378-1097(01)00328-7","journal-title":"FEMS Microbiol Lett"},{"key":"436_CR6","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1111\/j.1600-0463.2011.02846.x","volume":"120","author":"L Drago","year":"2012","unstructured":"Drago L, De Vecchi E, Torretta S et al (2012) Biofilm formation by bacteria isolated from upper respiratory tract before and after adenotonsillectomy. APMIS 120:410\u2013416. doi: 10.1111\/j.1600-0463.2011.02846.x","journal-title":"APMIS"},{"key":"436_CR7","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1128\/IAI.62.2.673-679.1994","volume":"62","author":"J Forsgen","year":"1994","unstructured":"Forsgen J, Samuelson A, Ahlin A et al (1994) Haemophilus influenzae resides and multiplies intracellularly in human adenoid tissue as demonstrated by in situ hybridization and bacterial viability assay. Infect Immun 62:673\u2013679","journal-title":"Infect Immun"},{"key":"436_CR8","doi-asserted-by":"crossref","first-page":"766","DOI":"10.3109\/00016489609137922","volume":"116","author":"J Forsgren","year":"1996","unstructured":"Forsgren J, Samuelson A, Borrelli S et al (1996) Persistence of nontypeable Haemophilus influenzae in adenoid macrophages: a putative colonization mechanism. Acta Otolaryngol 116:766\u2013773","journal-title":"Acta Otolaryngol"},{"key":"436_CR9","first-page":"134","volume":"27","author":"J Galli","year":"2007","unstructured":"Galli J, Cal\u00f3 L, Ardito F et al (2007) Biofilm formation by Haemophilus influenzae isolated from adeno-tonsil tissue samples, and its role in recurrent adenotonsillitis. Acta Otorhinolaryngol Ital 27:134\u2013138","journal-title":"Acta Otorhinolaryngol Ital"},{"key":"436_CR10","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1086\/521194","volume":"196","author":"N Heiniger","year":"2007","unstructured":"Heiniger N, Spaniol V, Troller R, Vischer M, Aebil Ch (2007) Reservoir of Moraxella catarrhalis in human pharyngeal lymphoid tissue. J Infect Dis 196:1080\u20131087A. doi: 10.1086\/521194","journal-title":"J Infect Dis"},{"key":"436_CR11","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1159\/000317635","volume":"6","author":"E Hertz\u00e9n","year":"2010","unstructured":"Hertz\u00e9n E, Johansson L, Wallin R et al (2010) M1 protein-dependent intracellular trafficking promotes persistence and replication of Streptococcus pyogenes in macrophages. J Innate Immun 6:534\u2013545. doi: 10.1159\/000317635","journal-title":"J Innate Immun"},{"key":"436_CR12","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1128\/JCM.38.2.818-825.2000","volume":"38","author":"M Hogardt","year":"2000","unstructured":"Hogardt M, Trebesius K, Geiger AM et al (2000) Specific and rapid detection by in situ hybridization of bacteria in clinical samples obtained from cystic fibrosis patients. J Clin Microbiol 38:818\u2013825","journal-title":"J Clin Microbiol"},{"key":"436_CR13","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.anl.2009.03.012","volume":"37","author":"M Hotomi","year":"2010","unstructured":"Hotomi M, Arai J, Billal DS et al (2010) Nontypeable Haemophilus influenzae isolated from intractable acute otitis media internalized into cultured human epithelial cells. Auris Nasus Larynx 37:137\u2013144. doi: 10.1016\/j.anl.2009.03.012","journal-title":"Auris Nasus Larynx"},{"key":"436_CR14","volume-title":"Clinical microbiology procedures handbook","author":"HD Isenberg","year":"2004","unstructured":"Isenberg HD (2004) Clinical microbiology procedures handbook, 2nd edn. American Society for Microbiology (ASM), Washington DC","edition":"2"},{"key":"436_CR15","author":"BC Kahl","year":"2013","unstructured":"Kahl BC (2013) Small colony variants (SCVs) of Staphylococcus aureus\u2013a bacterial survival strategy. Infect Genet Evol. doi: 10.1016\/j.meegid.2013.05.016","journal-title":"Infect Genet Evol"},{"key":"436_CR16","author":"R Khasriya","year":"2013","unstructured":"Khasriya R, Sathiananthamoorthy S, Ismail S et al (2013) The spectrum of bacterial colonisation associated with urothelial cells from patients with chronic lower urinary tract symptoms. J Clin Microbiol. doi: 10.1128\/JCM.03314-12","journal-title":"J Clin Microbiol"},{"key":"436_CR17","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1002\/alr.21117","volume":"3","author":"R Kim","year":"2013","unstructured":"Kim R, Freeman J, Waldvogel-Thurlow S, Roberts S, Douglas R (2013) The characteristics of intramucosal bacteria in chronic rhinosinusitis: a prospective cross-sectional analysis. Int Forum Allergy Rhinol 3:349\u2013354. doi: 10.1002\/alr.21117","journal-title":"Int Forum Allergy Rhinol"},{"key":"436_CR18","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1007\/s12223-010-0089-3","volume":"55","author":"O Melter","year":"2010","unstructured":"Melter O, Radojevi\u010d B (2010) Small colony variants of Staphylococcus aureus\u2013review. Folia Microbiol (Praha) 55:548\u2013558. doi: 10.1007\/s12223-010-0089-3","journal-title":"Folia Microbiol (Praha)"},{"key":"436_CR19","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1099\/mic.0.040451-0","volume":"157","author":"P Morey","year":"2011","unstructured":"Morey P, Cano V, Marti-Lliteras P et al (2011) Evidence for a non-replicative intracellular stage of nontypable Haemophilus influenzae in epithelial cells. Microbiology 157:234\u2013250. doi: 10.1099\/mic.0.040451-0","journal-title":"Microbiology"},{"key":"436_CR20","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/978-1-59745-523-7_12","volume":"493","author":"L Nistico","year":"2009","unstructured":"Nistico L, Gieseke A, Stoodley P, Hall-Stoodley L, Kerschner JE, Ehrlich GD (2009) Fluorescence \u201cin situ\u201d hybridization for the detection of biofilm in the middle ear and upper respiratory tract mucosa. Methods Mol Biol 493:191\u2013213. doi: 10.1007\/978-1-59745-523-7-12","journal-title":"Methods Mol Biol"},{"key":"436_CR21","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1128\/JCM.00756-10","volume":"49","author":"L Nistico","year":"2011","unstructured":"Nistico L, Kreft R, Gieseke A et al (2011) Adenoid reservoir for pathogenic biofilm bacteria. J Clinic Microbiol 49:1411\u20131420. doi: 10.1128\/JCM.00756-10","journal-title":"J Clinic Microbiol"},{"key":"436_CR22","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1078\/1438-4221-00253","volume":"293","author":"A Podbielski","year":"2003","unstructured":"Podbielski A, Beckert S, Schattke R et al (2003) Epidemiology and virulence gene expression of intracellular group A streptococci in tonsils of recurrently infected adults. Int J Med Microbiol 293:179\u2013190. doi: 10.1078\/1438-4221-00253","journal-title":"Int J Med Microbiol"},{"key":"436_CR23","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1038\/nrmicro1384","volume":"4","author":"RA Proctor","year":"2006","unstructured":"Proctor RA, von Eiff C, Kahl BC et al (2006) Small colony variants: a pathogenic form of bacteria that facilitates persistent and recurrent infections. Nat Rev Microbiol 4:295\u2013305. doi: 10.1038\/nrmicro1384","journal-title":"Nat Rev Microbiol"},{"key":"436_CR24","doi-asserted-by":"crossref","first-page":"6767","DOI":"10.1016\/j.atmosenv.2008.05.018","volume":"42","author":"T Rinsoz","year":"2008","unstructured":"Rinsoz T, Duquenne P, Greff-Mirguet G, Oppliger A (2008) Application of real-time PCR for total airborne bacterial assessment: comparison with epifluorescence microscopy and culture-dependent methods. Atmos Environ 42:6767\u20136774. doi: 10.1016\/j.atmosenv.2008.05.018","journal-title":"Atmos Environ"},{"key":"436_CR25","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.1111\/j.1398-9995.2010.02381.x","volume":"65","author":"F Sachse","year":"2010","unstructured":"Sachse F, Becker K, von Eiff C, Metze D, Rudack C (2010) Staphylococcus aureus invades the epithelium in nasal polyposis and induces IL-6 in nasal epithelial cells in vitro. Allergy 65:1430\u20131437. doi: 10.1111\/j.1398-9995.2010.02381.x","journal-title":"Allergy"},{"key":"436_CR26","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.ijmm.2009.08.019","volume":"300","author":"B Sinha","year":"2010","unstructured":"Sinha B, Fraunholz M (2010) Staphylococcus aureus host cell invasion and post-invasion events. Int J Med Microbiol 300:170\u2013175. doi: 10.1016\/j.ijmm.2009.08.019","journal-title":"Int J Med Microbiol"},{"key":"436_CR27","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1016\/j.micpath.2007.11.008","volume":"44","author":"M St\u0119pi\u0144ska","year":"2008","unstructured":"St\u0119pi\u0144ska M, Trafny EA (2008) Diverse type III secretion phenotypes among Pseudomonas aeruginosa strains upon infection of murine macrophage-like and endothelial cell lines. Microb Pathog 44:448\u2013458. doi: 10.1016\/j.micpath.2007.11.008","journal-title":"Microb Pathog"},{"key":"436_CR28","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1136\/jcp.2006.037309","volume":"60","author":"A Swidsinski","year":"2008","unstructured":"Swidsinski A, G\u00f6ktas O, Bessler C et al (2008) Spatial organisation of microbiota in quiescent adenoiditis and tonsillitis. J Clin Pathol 60:253\u2013260. doi: 10.1136\/jcp.2006.037309","journal-title":"J Clin Pathol"},{"key":"436_CR29","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1186\/1471-2431-11-94","volume":"11","author":"RB Thorton","year":"2011","unstructured":"Thorton RB, Rigby PJ, Wiertsema SP et al (2011) Multi-species bacterial biofilm and intracellular infection in otitis media. BMC Pediatr 11:94. doi: 10.1186\/1471-2431-11-94","journal-title":"BMC Pediatr"},{"key":"436_CR30","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.1128\/JCM.33.7.1835-1838.1995","volume":"33","author":"T Ueyama","year":"1995","unstructured":"Ueyama T, Kurono Y, Shirabe K, Takeshita M, Mogi G (1995) High incidence of Haemophilus influenzae in nasopharyngeal secretions and middle ear effusions as detected by PCR. J Clin Microbiol 33:1835\u20131838","journal-title":"J Clin Microbiol"},{"key":"436_CR31","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1001\/jama.287.13.1699","volume":"287","author":"S Vitovski","year":"2002","unstructured":"Vitovski S, Dunkin KT, Howard AJ et al (2002) Nontypeable Haemophilus influenzae in carriage and disease: a difference in IgA1 protease activity levels. JAMA 287:1699\u20131705. doi: 10.1001\/jama.287.13.1699","journal-title":"JAMA"},{"key":"436_CR32","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/S1369-5274(03)00054-7","volume":"6","author":"M Wagner","year":"2003","unstructured":"Wagner M, Horn M, Daims H (2003) Fluorescence in situ hybridization for the identification and characterization of prokaryotes. Curr Opin Microbiol 6:302\u2013309. doi: 10.1016\/S1369-5274(03)00054-7","journal-title":"Curr Opin Microbiol"},{"key":"436_CR33","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1001\/archoto.2009.186","volume":"135","author":"B Winther","year":"2009","unstructured":"Winther B, Gross BC, Henley JO, Early SV (2009) Location of bacterial biofilm in the mucus overlying the adenoid by light microscopy. Arch Otolaryngol Head Neck Surg 135:1239\u20131245. doi: 10.1001\/archoto.2009.186","journal-title":"Arch Otolaryngol Head Neck Surg"},{"key":"436_CR34","doi-asserted-by":"crossref","first-page":"e9452","DOI":"10.1371\/journal.pone.0009452","volume":"5","author":"A Zautner","year":"2010","unstructured":"Zautner A, Krause M, Stropahl G et al (2010) Intracellular persisting Staphylococcus aureus is the major pathogen in recurrent tonsillitis. PLoS ONE 5:e9452. doi: 10.1371\/journal.pone.0009452","journal-title":"PLoS ONE"}],"container-title":["Current Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00284-013-0436-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00284-013-0436-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00284-013-0436-0","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T18:54:48Z","timestamp":1596308088000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00284-013-0436-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8,10]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["436"],"URL":"https:\/\/doi.org\/10.1007\/s00284-013-0436-0","relation":{},"ISSN":["0343-8651","1432-0991"],"issn-type":[{"value":"0343-8651","type":"print"},{"value":"1432-0991","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8,10]]}}}